Research Square (Research Square),
Journal Year:
2022,
Volume and Issue:
unknown
Published: Dec. 16, 2022
Abstract
The
causative
agent
of
white
mold,
Sclerotinia
sclerotiorum
,
is
capable
infecting
over
600
plant
species
and
responsible
for
significant
crop
losses
across
the
globe.
Control
currently
dependent
on
broad-spectrum
chemical
agents
that
can
negatively
impact
agroecological
environment,
presenting
a
need
to
develop
alternative
control
measures.
In
this
study,
we
developed
transgenic
Arabidopsis
thaliana
(AT1703)
expressing
double-stranded
(ds)RNA
silence
S.
ABHYDROLASE-3
slow
infection
through
host
induced
gene
silencing
(HIGS).
Leaf
assays
show
reduced
lesion
size,
fungal
load,
transcript
abundance
in
AT1703
compared
wild-type
Col-0.
To
better
understand
how
HIGS
influences
host-pathogen
interactions,
performed
global
RNA
sequencing
Col-0
directly
at
site
infection.
data
reveals
enrichment
salicylic
acid
(SA)-mediated
systemic
acquired
resistance
(SAR)
pathway,
as
well
transcription
factors
predicted
regulate
immunity.
Using
qPCR,
identified
interacting
partners
polyamine
synthesis
pathway
demonstrate
co-reduction
with
levels
during
Together,
these
results
utility
technology
slowing
provide
insight
into
role
A.
–
pathosystem.
Scientific Reports,
Journal Year:
2023,
Volume and Issue:
13(1)
Published: April 20, 2023
Abstract
The
causative
agent
of
white
mold,
Sclerotinia
sclerotiorum
,
is
capable
infecting
over
600
plant
species
and
responsible
for
significant
crop
losses
across
the
globe.
Control
currently
dependent
on
broad-spectrum
chemical
agents
that
can
negatively
impact
agroecological
environment,
presenting
a
need
to
develop
alternative
control
measures.
In
this
study,
we
developed
transgenic
Arabidopsis
thaliana
(AT1703)
expressing
hairpin
(hp)RNA
silence
S.
ABHYDROLASE-3
slow
infection
through
host
induced
gene
silencing
(HIGS).
Leaf
assays
show
reduced
lesion
size,
fungal
load,
transcript
abundance
in
AT1703
compared
wild-type
Col-0.
To
better
understand
how
HIGS
influences
host–pathogen
interactions,
performed
global
RNA
sequencing
Col-0
directly
at
site
infection.
data
reveals
enrichment
salicylic
acid
(SA)-mediated
systemic
acquired
resistance
(SAR)
pathway,
as
well
transcription
factors
predicted
regulate
immunity.
Using
RT-qPCR,
identified
interacting
partners
polyamine
synthesis
pathway
demonstrate
co-reduction
with
levels
during
Together,
these
results
utility
technology
slowing
provide
insight
into
role
A.
–
pathosystem.
PLoS Pathogens,
Journal Year:
2025,
Volume and Issue:
21(5), P. e1013129 - e1013129
Published: May 2, 2025
Sclerotinia
stem
rot
is
a
devastating
disease
affecting
vegetables
and
oil
crops
worldwide.
It
caused
by
the
necrotrophic
ascomycete
(
S.
)
sclerotiorum.
Host-induced
gene
silencing
(HIGS)
has
shown
promise
in
control
against
insects
fungal
pathogens,
but
effective
HIGS
target
genes
sclerotiorum
remain
limited.
In
this
study,
we
identified
GDP-mannose
pyrophosphorylase
(GMPP)
SsMPG2
through
forward
genetic
analysis.
Ssmpg2
mutants
exhibit
abnormal
sclerotia
compound
appressoria,
along
with
defective
cell
wall
integrity
attenuated
virulence.
Meanwhile,
knocking
out
reduced
GMPP
activity
glycosylation
of
proteins.
addition,
interacts
SsMPG1,
which
essential
.
Downstream
SsMPG1-SsMPG2
complex,
SsPMT4
,
encodes
an
O-mannosyltransferase,
also
critical
for
appressoria
formation
Notably,
MPG2
virulence
several
other
pathogens
such
as
Botrytis
cinerea
Magnaporthe
oryzae
Fusarium
graminearum
Furthermore,
expressing
hairpin
RNAs
SsMPG1
Nicotiana
benthamiana
Arabidopsis
thaliana
significantly
symptoms
Collectively,
our
findings
demonstrate
roles
phytopathogenic
fungi
suggest
that
MPGs
are
promising
HlGS
targets
controlling
The Plant Journal,
Journal Year:
2023,
Volume and Issue:
118(2), P. 324 - 344
Published: Dec. 27, 2023
Sclerotinia
sclerotiorum
causes
white
mold
or
stem
rot
in
a
wide
range
of
economically
important
plants,
bringing
significant
yield
losses
worldwide.
Control
this
pathogen
is
difficult
as
its
resting
structure
sclerotia
can
survive
soil
for
years,
and
no
Resistance
genes
have
been
identified
S.
hosts.
Host-induced
gene
silencing
(HIGS)
has
shown
promising
effects
controlling
many
fungal
pathogens,
including
sclerotiorum.
However,
better
molecular
genetic
understanding
signaling
pathways
involved
development
pathogenicity
needed
to
provide
effective
HIGS
targets.
Here,
by
employing
forward
screen,
we
characterized
an
evolutionarily
conserved
mitogen-activated
protein
kinase
(MAPK)
cascade
sclerotiorum,
consisting
SsSte50-SsSte11-SsSte7-Smk1,
which
controls
mycelial
growth,
development,
compound
appressoria
formation,
virulence,
hyphal
fusion.
Moreover,
disruption
the
putative
downstream
transcription
factor
SsSte12
led
normal
but
deformed
attenuated
host
penetration,
well
impaired
apothecia
suggestive
diverged
regulation
MAPK
cascade.
Most
importantly,
targeting
SsSte50
using
host-expressed
double-stranded
RNA
resulted
largely
reduced
virulence
on
both
Nicotiana
benthamiana
leaves
transgenic
Arabidopsis
thaliana
plants.
Therefore,
generally
pathogenesis
serve
ideal
targets
mitigating
economic
damages
caused
infection.
Pathogens,
Journal Year:
2025,
Volume and Issue:
14(4), P. 398 - 398
Published: April 21, 2025
Sclerotinia
sclerotiorum
is
a
globally
widespread
and
vast
destructive
plant
pathogenic
fungus
that
causes
significant
yield
losses
in
crops.
Due
to
the
lack
of
effective
resistant
germplasm
resources,
control
diseases
caused
by
S.
largely
relies
on
chemical
fungicides.
However,
excessive
use
these
chemicals
not
only
environmental
concerns
but
also
leads
increased
development
resistance
sclerotiorum.
In
contrast,
trans-kingdom
sRNA
silencing-based
technologies,
such
as
host-induced
gene
silencing
(HIGS)
spray-induced
(SIGS),
offer
novel,
effective,
environmentally
friendly
methods
for
management
infection.
This
review
summarizes
recent
advances
identification
genes,
target
selection,
categories,
application
RNA
interference
(RNAi)
technologies
targeting
this
pathogen.
Although
some
challenges,
including
off-target
effects
efficiency
external
uptake,
exist,
findings
have
proposed
solutions
further
improvement.
Combined
with
latest
developments
CRISPR/Cas
editing
other
RNAi
has
potential
become
crucial
tool
sclerotinia
stem
rot
(SSR),
mitigating
impact
crop
production.
