Research Square (Research Square),
Journal Year:
2022,
Volume and Issue:
unknown
Published: Oct. 27, 2022
Abstract
Chilling
requirement
(CR)
is
an
important
agronomic
trait
controlling
the
floral
bud
break
for
proper
flowering
in
peach.
Even
though
it
has
been
widely
researched
and
several
peach
CR
quantitative
loci
(QTLs)
have
identified,
no
diagnostic
DNA
tests
validated
U.S.
breeding
germplasm
are
available
this
trait.
Breeders
growers
need
a
simple
test
to
predict
of
cultivars
their
particular
environment.
Therefore,
we
developed
quick
reliable
Kompetitive
Allele
Specific
PCR
(KASP)
using
haplotype
information
from
9K
IPSC
genotype
data
integrating
four
CR-associated
SNP
markers
previously
reported
QTL
region
on
linkage
group
1.
Four
KASP
assays
(Ppe.CR.1–1
-4)
were
77
cultivars,
nine
accessions
two
F
2
populations,
with
96
74%
accuracy
predicting
correct
phenotype,
respectively.
Furthermore,
Ppe.CR.1
showed
80%
precise
phenotype
Clemson
University
material.
Only
one
assay
(Ppe.CR.1–1)
needed
distinguish
between
haplotypes
lower
higher
than
800
chilling
hours,
(Pp.CR.1–1
−
4),
capable
distinguishing
low,
moderate,
high
alleles.
Coupled
crude
extraction,
provides
low-cost
option
breeders
material
more
70%
accuracy.
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Jan. 17, 2024
Abstract
Genomic
regions
associated
with
ripening
time
(RPT)
and
soluble
solids
concentration
(SSC)
were
mapped
using
a
pedigreed
population
including
multiple
F
1
2
families
from
the
Clemson
University
peach
breeding
program
(CUPBP).
RPT
SSC
QTLs
consistently
identified
in
two
seasons
(2011
2012)
average
datasets
(average
of
seasons).
A
target
region
spanning
10,981,971–11,298,736
bp
on
chromosome
4
reference
genome
used
for
haplotype
analysis
revealed
four
haplotypes
significant
differences
trait
values
among
different
diplotype
combinations.
Favorable
alleles
at
both
determined
DNA
test
predicting
was
developed.
Two
Kompetitive
Allele
Specific
PCR
(KASP)
assays
validated
84
cultivars
163
seedlings
CUPBP,
only
one
assay
(
Ppe.RPT/SSC-1
)
needed
to
predict
between
early
late-season
low
high
SSC.
These
results
advance
our
understanding
genetic
basis
facilitate
selection
new
desired
Frontiers in Plant Science,
Journal Year:
2025,
Volume and Issue:
15
Published: Jan. 8, 2025
Cymbidium
ensifolium
(L.)
Sw.
is
a
valuable
ornamental
plant
in
the
genus
Cymbidium,
family
Orchidaceae,
with
high
economic
and
ecological
significance.
However,
lack
of
population
genetic
information
molecular
markers
has
hindered
development
sales
market
breeding
C.
despite
abundance
commercial
cultivars
available.
In
this
study,
we
aimed
to
develop
set
single
nucleotide
polymorphism
(SNP)
distinguish
main
cultivated
China
provide
technical
support
for
domestic
cultivar
protection,
registration,
rights
protection.
A
total
1,280,516
high-quality
loci
were
identified
from
10,021,591
SNPs
obtained
by
sequencing
50
using
double
digest
restriction
site-assisted
DNA
technology.
7,599
selected
kompetitive
allele-specific
PCR
(KASP)
primer
design,
4,360
successfully
designed
as
KASP
markers.
Population
structure
analysis
revealed
that
best
divided
into
four
populations,
some
correlation
between
group
distribution
morphological
geographical
characteristics
germplasm.
Using
genotyping
results
28
screened
cultivars,
minimum
11
was
could
83
completely,
remaining
17
serving
extended
The
average
PIC
value
0.345,
which
considered
medium
polymorphism.
fingerprints
constructed
on
basis
markers,
providing
new
approach
mapping
efficiency,
accuracy,
low
cost
compared
traditional
methods.
Tree Genetics & Genomes,
Journal Year:
2023,
Volume and Issue:
19(2)
Published: Feb. 7, 2023
Abstract
Almond
shell
hardness
is
an
economically
important
quality
trait
that
protects
the
integrity
of
kernel.
It
directly
related
with
shelling
or
crack-out
percent.
In
U.S.
and
Australian
markets,
soft
to
semi-hard
shells
varieties
have
been
used
in
industry,
while
European
market,
hard
are
dominantly
sold
distributed.
The
origin
a
from
wild
almond
species.
Soft
USA
Australia
selected
for
higher
percent
larger
kernel
ease
processing.
Here,
we
examined
breeding
populations
totaling
264
trees
were
genotyped
genotyping-by-sequencing
(GBS)
where
was
measured
texture
analyzer,
weight,
in-shell
weights
thickness
calculated.
genome-wide
association,
seven
genetic
loci
found
be
associated
hardness,
percent,
weight.
Of
these
seven,
one
shared
between
multiple
traits.
Kompetitive
allele-specific
PCR
(KASP)
assays
developed
tested
unrelated
lines
Australia,
Spain,
France
Italy.
One
KASP
assay
highly
predictive
this
diverse
panel
can
applied
use
marker-assisted
breeding.
Modern
crop
improvement
programs
are
based
on
molecular
breeding,
and
the
main
element
of
breeding
is
markers.
Molecular
marker-assisted
techniques
enable
plant
breeders
to
quickly
accurately
identify
enhance
traits
interest.
KASP,
or
competitive
allele-specific
PCR,
most
recent
development
in
field
markers-aided
technology.
KASP
a
high-throughput,
flexible,
cost-effective,
intelligent
genotyping
method.
has
vivid
use
agriculture,
animal
sciences,
also
human
disease
diagnosis.
Compared
other
techniques,
provides
accurate
precise
outcomes
within
short
possible
time
cost-effective
manner.
The
technology
fluorescence
resonance
energy
transfer
oligo
extension
system;
thus,
does
not
require
laborious
gel
electrophoresis
system.
Due
these
advantages,
drawn
much
attention
from
researchers
already
established
itself
as
global
benchmark
This
chapter
describes
concepts,
fundamentals,
working
principles,
applications,
prospects
technology,
well
comparisons
with
platforms
currently
used
programs.
Scientific Reports,
Journal Year:
2023,
Volume and Issue:
13(1)
Published: Jan. 18, 2023
Abstract
Chilling
requirement
(CR)
is
an
important
agronomic
trait
controlling
the
floral
bud
break
for
proper
flowering
in
peach.
Even
though
it
has
been
widely
researched
and
several
peach
CR
quantitative
loci
(QTLs)
have
identified,
no
diagnostic
DNA
tests
validated
U.S.
breeding
germplasm
are
available
this
trait.
Breeders
growers
need
a
simple
test
to
predict
of
cultivars
their
particular
environment.
Therefore,
we
developed
quick
reliable
Kompetitive
Allele
Specific
PCR
(KASP)
using
haplotype
information
from
9K
IPSC
genotype
data
integrating
four
CR-associated
SNP
markers
previously
reported
QTL
region
on
linkage
group
1.
Four
KASP
assays
(Ppe.CR.1-1
-4)
were
77
cultivars,
nine
accessions
two
F
2
populations,
with
96
74%
accuracy
determining
expected
(compared
array)
predicting
phenotype,
respectively.
Furthermore,
Ppe.CR.1
showed
80%
precise
phenotype
Clemson
University
material.
Only
one
assay
(Ppe.CR.1-1)
needed
distinguish
between
haplotypes
lower
higher
than
800
chilling
hours,
(Pp.CR.1-1
-4),
capable
distinguishing
low,
moderate,
high
alleles.
Coupled
crude
extraction,
provides
low-cost
option
breeders
material
more
70%
accuracy.
