Laboratory Investigation, Journal Year: 2024, Volume and Issue: 104(12), P. 102150 - 102150
Published: Oct. 10, 2024
Language: Английский
Journal of Hematology & Oncology, Journal Year: 2024, Volume and Issue: 17(1)
Published: Aug. 12, 2024
Long non-coding RNAs (lncRNAs), once considered transcriptional noise, have emerged as critical regulators of gene expression and key players in cancer biology. Recent breakthroughs revealed that certain lncRNAs can encode small open reading frame (sORF)-derived peptides, which are now understood to contribute the pathogenesis various cancers. This review synthesizes current knowledge on detection, functional roles, clinical implications lncRNA-encoded peptides cancer. We discuss technological advancements detection validation sORFs, including ribosome profiling mass spectrometry, facilitated discovery these peptides. The roles processes such transcription, translation regulation, signal transduction, metabolic reprogramming explored types potential is highlighted, with a focus their utility diagnostic biomarkers, prognostic indicators, therapeutic targets. challenges future directions translating findings into practice also discussed, need for large-scale validation, development sensitive methods, optimization peptide stability delivery.
Language: Английский
Citations
15
Molecular Cancer, Journal Year: 2024, Volume and Issue: 23(1)
Published: Sept. 30, 2024
Language: Английский
Citations
15
Amino Acids, Journal Year: 2024, Volume and Issue: 56(1)
Published: Feb. 13, 2024
Abstract The advance of high-throughput sequencing enhances the discovery short ORFs embedded in long non-coding RNAs (lncRNAs). Here, we uncovered production and biological activity lncRNA-hidden polypeptides lung adenocarcinoma (LUAD). In present study, bioinformatics was used to screen LUAD. Analysis protein expression done by western blot or immunofluorescence assay. functions polypeptide were determined detecting its effects on cell viability, proliferation, migration, invasion, pemetrexed (PEM) sensitivity. interactors analyzed mass spectrometry after Co-immunoprecipitation (Co-IP) results showed that lncRNA LINC00954 confirmed encode a novel LINC00954-ORF. had tumor-suppressor features A549 cells repressing growth, motility invasion. Moreover, enhanced PEM sensitivity suppressed growth A549/PEM cells. this close correlations with RNA processing, amide metabolic process, translation, binding, transport, DNA replication. As conclusion, LINC00954-ORF possesses PEM-resistant sensitizes PEM, providing evidence is potential anti-cancer agent
Language: Английский
Citations
4
Non-coding RNA Research, Journal Year: 2024, Volume and Issue: 10, P. 231 - 241
Published: Oct. 30, 2024
Language: Английский
Citations
4
Journal of Hematology & Oncology, Journal Year: 2025, Volume and Issue: 18(1)
Published: Feb. 19, 2025
In the present era, noncoding RNAs (ncRNAs) have become a subject of considerable scientific interest, with peptides encoded by ncRNAs representing particularly promising avenue investigation. The identification ncRNA-encoded in human cancers is increasing. These regulate cancer progression through multiple molecular mechanisms. Here, we delineate patterns diverse and provide synopsis methodologies employed for that possess capacity to encode these peptides. Furthermore, discuss impacts on biological behavior cells underlying conclusion, describe prospects challenges need be overcome.
Language: Английский
Citations
0
Molecular Cancer, Journal Year: 2025, Volume and Issue: 24(1)
Published: April 2, 2025
The human genome harbors approximately twenty thousand protein-coding genes, and a significant portion of life science research focuses on elucidating their functions the underlying mechanisms. Recent studies have revealed that small open reading frame (sORF), originating from non-coding RNAs or 5' leader sequences messenger RNAs, can be translated into peptides called microproteins through cap-dependent cap-independent These interact with diverse molecular partners to modulate gene expression at multiple regulatory levels, thereby playing critical roles in various biological processes. Notably, sORF-encoded exhibit aberrant patterns cancer are implicated tumor initiation progression, expanding our understanding biology. In this review, we introduce translational mechanisms identification methods microproteins, summarize dysregulation regulating expression, emphasize driving hallmark events cancer. Furthermore, discuss clinical significance as diagnostic prognostic biomarkers, well therapeutic targets.
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3651 - 3651
Published: April 12, 2025
Micropeptides (miPEPs), encoded by short open reading frames (sORFs) within various genomic regions, have recently emerged as critical regulators of multiple biological processes. In particular, these small molecules are now increasingly being recognized for their role in modulating viral replication, pathogenesis, and host immune responses. Both miPEPs virus-derived been noted ability to regulate virus–host interactions through diversified mechanisms such altering protein stability protein–protein interactions. Although thousands sORFs annotated having the potential encode miPEPs, only a number experimentally validated so far, with some directly linked subset associated modulation, indicating that investigation is still its infancy. The systematic identification, translational status assessment, in-depth characterization, functional analysis substantial fraction encoding remain largely underexplored. Further studies anticipated uncover intricate underlying interactions, broader functions miPEPs. This article will review emerging roles immunity, discuss challenges future perspectives miPEP studies.
Language: Английский
Citations
0
Food Research International, Journal Year: 2025, Volume and Issue: 211, P. 116496 - 116496
Published: April 18, 2025
Language: Английский
Citations
0
Cell Death Discovery, Journal Year: 2025, Volume and Issue: 11(1)
Published: April 21, 2025
Abstract Cancer, uncontrolled cell growth due to the loss of cycle regulation, is often found be associated with viral infections and, as recent studies show, bacterial well. Emerging reports also suggest a strong link between fungi and cancer. The crucial virulence trait fungi, switch from yeast (Y) hyphal (H) form, carcinogenesis. physicochemical properties signal transduction pathways involved in form overlap those tumor formation. Inhibiting differentiation causes apoptosis whereas preventing leads cancer multicellular organisms. Literature on fungi-cancer linkage, though limited, increasing rapidly. This review examines cancer-specific fungal communities, impact microbiome progression, similarities cells turning cancerous at biochemical molecular levels, including overlaps Based available evidence, we that molecules inhibiting yeast-hyphal transition can combined targeting for effective treatment. points out fertile research areas where mycologists researchers collaborate unravel common mechanisms. Moreover, antibodies fungal-specific chitin glucan used selective neutralization cells. These new combinations potential therapies are expected facilitate development target-specific, less harmful commercially feasible anticancer therapies.
Language: Английский
Citations
0
International Journal of Cancer, Journal Year: 2025, Volume and Issue: unknown
Published: April 25, 2025
Abstract Micropeptides are commonly identified as peptides encoded by non‐coding RNAs (ncRNAs). In the short open reading frame (sORF) of ncRNAs, there is a base sequence encoding functional micropeptides, which great significance in biological field. Recently, micropeptides regulate diverse processes, including mitochondrial metabolism, calcium transport, mRNA splicing, signal transduction, myocyte fusion, and cellular senescence, regulating homeostasis internal environment cancer's incidence progression. Especially, study cancer about potential regulatory mechanism will be conducive to further understanding process initiation development. More more research shows have been confirmed play an essential role emergence multiple kinds cancers, Breast cancer, Colon Colorectal Glioma, Glioblastoma, Liver cancer. This review presents comprehensive synthesis latest advancements our roles cells, with particular focus on networks involving oncogenesis. The new mode action provides innovative ideas for diagnosis treatment. Moreover, we explored significant capacity diagnostic biomarkers targets anti‐cancer therapies clinical settings, highlighting their development micropeptide‐based tools therapeutics.
Language: Английский
Citations
0