Variations in extracellular vesicle shedding of Cystoisospora suis stages (Apicomplexa: Coccidia)
International Journal for Parasitology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
Cystoisospora
suis,
a
porcine
enteral
parasite
of
the
order
Coccidia,
is
characterized
by
complex
life
cycle,
with
asexual
and
sexual
development
in
epithelium
host
gut
an
environmental
phase
as
oocyst.
All
developmental
stages
vary
greatly
their
morphology
function,
therefore
excrete
different
bioactive
molecules
for
intercellular
communication.
Due
to
development,
we
hypothesized
that
extracellular
vesicles
(EVs)
cargo
highly
dependent
on
cycle
from
which
they
are
released.
This
study
aimed
characterize
compare
EVs
all
C.
suis.
Nanoparticle
tracking
analysis
microscopy
were
used
determine
particle
numbers
size
distributions
stage-specific
EVs.
Furthermore,
Fourier-transform
infrared
spectral
was
employed
metabolic
fingerprinting
EVs,
lipid
protein
profiles
determined.
Overall,
revealed
asexual,
transmissible
suis
release
during
parasite's
cycle.
endogenous
found
be
more
similar
each
other
than
those
stage,
ratio
fatty
acids
polysaccharides
proteins
changed
development.
In
particular,
associated
Apicomplexa
involved
vesicle
shedding
showed
changes
expression
stages.
Lipid
same
concentration
through
stages,
whereas
amount
stereolipids,
sphingolipids
glycerolipids
between
conclusion,
this
study,
presents
first
known
characterization
demonstrates
link
respective
parasite,
putative
functions
parasite-parasite
host-parasite
interplays.
Language: Английский
An in-depth exploration of the multifaceted roles of EVs in the context of pathogenic single-cell microorganisms
Microbiology and Molecular Biology Reviews,
Journal Year:
2024,
Volume and Issue:
unknown
Published: June 13, 2024
SUMMARY
Extracellular
vesicles
(EVs)
have
been
recognized
throughout
scientific
communities
as
potential
vehicles
of
intercellular
communication
in
both
eukaryotes
and
prokaryotes,
thereby
influencing
various
physiological
pathological
functions
parent
recipient
cells.
This
review
provides
an
in-depth
exploration
the
multifaceted
roles
EVs
context
bacteria
protozoan
parasite
EVs,
shedding
light
on
their
contributions
to
processes
disease
pathogenesis.
These
studies
highlight
a
conserved
mechanism
cellular
communication,
which
may
lead
us
important
breakthroughs
our
understanding
infection,
mechanisms
pathogenesis,
indicators
disease.
Furthermore,
are
involved
host–microbe
interactions,
offering
insights
into
strategies
employed
by
parasites
modulate
host
responses,
evade
immune
system,
establish
infections.
Language: Английский
Faecal Short-Chain, Long-Chain, and Branched-Chain Fatty Acids as Markers of Different Chronic Inflammatory Enteropathies in Dogs
Animals,
Journal Year:
2024,
Volume and Issue:
14(12), P. 1825 - 1825
Published: June 19, 2024
Chronic
inflammatory
enteropathies
(CIEs)
are
classified
based
on
treatment
trials,
and
new
methods
being
sought
for
earlier
differentiation
characterization.
Giardia
infection
(GIA)
is
one
of
the
first
differential
diagnoses
may
be
present
in
CIE-affected
dogs.
The
aim
this
study
was
to
evaluate
faecal
characteristics
fatty
acid
profile
(short,
medium,
long,
branched-chain
acids)
dogs
with
food-responsive
enteropathy
(FRE),
immunosuppressant-responsive
(IRE),
infected
compared
healthy
control
(HC)
animals
as
a
potential
non-invasive
indicator
intestinal
health
that
helps
CIEs.
C16:1n-7
percentage
(p
=
0.0001)
C16:1n-7/C16:0
ratio
served
differentiate
between
HC,
FRE,
IRE.
IRE
presented
lower
levels
short-chain
acids
(∑SCFAs)
0.0008)
acetic
(C2)
0.0007)
other
three
groups
propionic
(C3)
0.0022)
HCs.
GIA
higher
fat
content
0.0080)
iso/anteiso
(BCFAs)
HC
FRE.
Correlations
some
desaturation
indices
canine
bowel
disease
activity
index
were
observed,
suggesting
these
compounds
could
play
an
important
role
pathogenesis
diseases.
Language: Английский
Overview of extracellular vesicles in pathogens with special focus on human extracellular protozoan parasites
Memórias do Instituto Oswaldo Cruz,
Journal Year:
2024,
Volume and Issue:
119
Published: Jan. 1, 2024
Extracellular
vesicles
(EVs)
are
lipid-bilayered
membrane-delimited
particles
secreted
by
almost
any
cell
type,
involved
in
different
functions
according
to
the
of
origin
and
its
state.
From
these,
communication,
pathogen-host
interactions
modulation
immune
response
have
been
widely
studied.
Moreover,
these
could
be
employed
for
diagnostic
therapeutic
purposes,
including
infections
produced
pathogens
diverse
types;
regarding
parasites,
secretion,
characterisation,
roles
EVs
studied
particular
cases.
heterogeneity
presents
challenges
at
every
stage
studies,
which
motivates
research
this
area.
In
review,
we
summarise
some
aspects
related
secretion
from
several
groups
pathogens,
with
special
focus
on
most
recent
extracellular
protozoan
parasites.
Language: Английский