Decoding the Tumor Microenvironment of Myoepithelial Cells in Triple‐Negative Breast Cancer Through Single‐Cell and Transcriptomic Sequencing and Establishing a Prognostic Model Based on Key Myoepithelial Cell Genes

International Journal of Genomics, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

Background: Triple‐negative breast cancer (TNBC) is an aggressive subtype with high malignancy, rapid progression, and a poor 5‐year survival rate of ~77%. Due to the lack targeted therapies, treatment options are limited, highlighting urgent need for novel therapeutic strategies. Myoepithelial cells (MECs) in tumor microenvironment may significantly influence TNBC development progression. Methods: This study used single‐cell RNA sequencing compare MEC gene expression normal versus tissues. TNBC‐associated MECs showed increased proliferation‐ immune‐related genes (e.g., FDCSP, KRT14, KRT17) decreased inflammatory extracellular matrix‐related CXCL8, SRGN, DCN). Copy number variation pseudotime analyses revealed genomic alterations phenotypic dynamics MECs. A CoxBoost‐based prognostic model was developed validated across 20 cohorts, integrating immune profiling, pathway enrichment, drug sensitivity analyses. Mendelian randomization identified TPD52 as risk–associated gene. siRNA knockdown performed cell lines evaluate its effects on proliferation migration. Results: displayed significant changes integrity, impacting responses invasion. The effectively predicted 1‐, 3‐, outcomes, stratifying high‐risk patients enriched cycle DNA replication pathways, reduced checkpoint expression, chemotherapy resistance. tumor‐promoting gene, suppressed Conclusion: highlights MECs’ role provides CoxBoost personalized treatment, identifies potential target intervention.

Language: Английский

Analyzing the potential targets and mechanisms of chronic kidney disease induced by common synthetic Endocrine Disrupting Compounds (EDCs) in Chinese surface water environment using network toxicology and molecular docking techniques

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 958, P. 177980 - 177980

Published: Dec. 9, 2024

Language: Английский