Fast and easy bioassay for the necrotizing fungus Botrytis cinerea on poplar leaves DOI Creative Commons
Steven Dreischhoff, Ishani Shankar Das,

Felix Häffner

et al.

Plant Methods, Journal Year: 2023, Volume and Issue: 19(1)

Published: March 29, 2023

Necrotizing pathogens pose an immense economic and ecological threat to trees forests, but the molecular analysis of these is still in its infancy because lacking model systems. To close this gap, we developed a reliable bioassay for widespread necrotic pathogen Botrytis cinerea on poplars (Populus sp.), which are established organisms study tree biology.Botrytis was isolated from Populus x canescens leaves. We infection system using fungal agar plugs, easy handle. The method does not require costly machinery results very high success significant proliferation within four days. successfully tested plug 18 poplar species five different sections. Emerging necroses were phenotypically anatomically examined adapted methods image analyses areas. calibrated B. DNA against Ct-values obtained by quantitative real-time polymerase chain reaction measured amounts infected Increases area strictly correlated first days after inoculation. Methyl jasmonate pretreatment leaves decreased spreading infection.We provide simple rapid protocol effects necrotizing quantification set stage in-depth studies immunity resistance generalist trees.

Language: Английский

Killing softly: a roadmap of Botrytis cinerea pathogenicity DOI Creative Commons
Kai Bi, Yong Liang, Tesfaye Mengiste

et al.

Trends in Plant Science, Journal Year: 2022, Volume and Issue: 28(2), P. 211 - 222

Published: Sept. 30, 2022

Botrytis cinerea, a widespread plant pathogen with necrotrophic lifestyle, causes gray mold disease in many crops. Massive secretion of enzymes and toxins was long considered to be the main driver infection, but recent studies have uncovered rich toolbox for B. cinerea pathogenicity. The emerging picture is multilayered infection process governed by exchange factors that collectively contribute development. No shows complete resistance against pattern-triggered immune responses potential significantly reduce progression, opening new possibilities producing cinerea-tolerant plants. We examine current models, highlight knowledge gaps, suggest directions future studies.

Language: Английский

Citations

163
Fungal biotechnology: From yesterday to tomorrow DOI Creative Commons
Mitchell G. Roth, Nathaniel Westrick, Thomas Baldwin

et al.

Frontiers in Fungal Biology, Journal Year: 2023, Volume and Issue: 4

Published: March 27, 2023

Fungi have been used to better the lives of everyday people and unravel mysteries higher eukaryotic organisms for decades. However, comparing progress development stemming from fungal research that human, plant, bacterial research, fungi remain largely understudied underutilized. Recent commercial ventures begun gain popularity in society, providing a new surge interest fungi, mycelia, potential applications these various aspects research. Biotechnological advancements cannot occur without intensive amounts time, investments, tool development. In this review, we highlight past breakthroughs biotechnology, discuss requirements advance biotechnology even further, touch on horizon with highest positively impact both society.

Language: Английский

Citations

36
Surface frustration re-patterning underlies the structural landscape and evolvability of fungal orphan candidate effectors DOI Creative Commons
Mark C. Derbyshire, Sylvain Raffaele

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Aug. 28, 2023

Pathogens secrete effector proteins to subvert host physiology and cause disease. Effectors are engaged in a molecular arms race with the resulting conflicting evolutionary constraints manipulate cells without triggering immune responses. The mechanisms allowing effectors be at same time robust evolvable remain largely enigmatic. Here, we show that 62 conserved structure-related families encompass majority of fungal orphan candidates Pezizomycotina subphylum. These diversified through changes patterns thermodynamic frustration surface residues. underlying mutations tended increase robustness overall protein structure while switching potential binding interfaces. This mechanism could explain how maintained biological activity over long timespans different environments provides model for emergence sequence-unrelated structures.

Language: Английский

Citations

31
Advances and Challenges in CRISPR/Cas-Based Fungal Genome Engineering for Secondary Metabolite Production: A Review DOI Creative Commons
Duoduo Wang,

Shunda Jin,

Qianhui Lu

et al.

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(3), P. 362 - 362

Published: March 15, 2023

Fungi represent an important source of bioactive secondary metabolites (SMs), which have wide applications in many fields, including medicine, agriculture, human health, and other industries. The genes involved SM biosynthesis are usually clustered adjacent to each into a region known as biosynthetic gene cluster (BGC). recent advent diversity genetic genomic technologies has facilitated the identification cryptic or uncharacterized BGCs their associated SMs. However, there still challenges that hamper broader exploration industrially metabolites. advanced CRISPR/Cas system revolutionized fungal engineering enabled discovery novel compounds. In this review, we firstly introduce relationships with SMs, followed by brief summary conventional strategies for engineering. Next, range state-of-the-art CRISPR/Cas-based tools been developed review these methods fungi research on Finally, limitations systems discussed directions future proposed order expand improve efficiency

Language: Английский

Citations

27
Botrytis cinerea combines four molecular strategies to tolerate membrane-permeating plant compounds and to increase virulence DOI Creative Commons

Yaohua You,

H. M. Suraj,

Linda Matz

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 31, 2024

Saponins are plant secondary metabolites comprising glycosylated triterpenoids, steroids or steroidal alkaloids with a broad spectrum of toxicity to microbial pathogens and pest organisms that contribute basal defense biotic attack. Secretion glycosyl hydrolases enzymatically convert saponins into less toxic products was thus far the only mechanism reported enable fungal colonize their saponin-containing host plant(s). We studied mechanisms fungus Botrytis cinerea utilizes be tolerant well-characterized, structurally related from tomato Digitalis purpurea. By gene expression studies, comparative genomics, enzyme assays testing large panel (knockout complemented) mutants, we unraveled four distinct cellular participate in mitigation activity these virulence on saponin-producing plants. The enzymatic deglycosylation identified is novel unique this fungus-saponin combination. other three tolerance operate membrane mediated by protein families widely distributed kingdom. present spatial temporal model how jointly confer discuss repercussions findings for pathogenic fungi, as well human pathogens. Plant antimicrobial toxicity. Here, You et al. characterize which establishes saponins.

Language: Английский

Citations

11
Global Transcriptome Analysis of the Peach (Prunus persica) in the Interaction System of Fruit–Chitosan–Monilinia fructicola DOI Creative Commons
Polina C. Tsalgatidou, Anastasia Boutsika, A. Papageorgiou

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(5), P. 567 - 567

Published: Feb. 20, 2024

The peach (Prunus persica L.) is one of the most important stone-fruit crops worldwide. Nevertheless, successful fruit production seriously reduced by losses due to Monilinia fructicola causal agent brown rot. Chitosan has a broad spectrum antimicrobial properties and may also act as an elicitor that activate defense responses in plants. As little known about elicitation potential chitosan fruits its impact at their transcriptional-level profiles, aim this study was uncover using RNA-seq induced regulated action fruit–chitosan–M. interaction. Samples were obtained from treated with or inoculated M. fructicola, well pre-treated thereafter fungus. found delay postharvest decay fruits, expression profiles showed defense-priming effects mainly evident after pathogen challenge, driven particularly modulations differentially expressed genes (DEGs) related cell-wall modifications, perception, signal transduction, preventing spread In contrast, compatible interaction challenged, shift towards triggered delay, which insufficient limit fungal expansion, whereas DEGs involved particular processes have facilitated early colonization. Physiological indicators measured. Additionally, highlight direct activity against Overall, results clarify possible mechanisms chitosan-mediated tolerance set new foundations for employment control rot peaches.

Language: Английский

Citations

10
Recent advances in the application of CRISPR/Cas-based gene editing technology in Filamentous Fungi DOI
Xuhong Li, Huizhi Lu,

Jingwu Yao

et al.

Biotechnology Advances, Journal Year: 2025, Volume and Issue: 81, P. 108561 - 108561

Published: March 12, 2025

Language: Английский

Citations

1
From Genes to Molecules, Secondary Metabolism in Botrytis cinerea: New Insights into Anamorphic and Teleomorphic Stages DOI Creative Commons
Haroldo da Silva Ripardo-Filho, Víctor Coca-Ruíz, Ivonne Suárez

et al.

Plants, Journal Year: 2023, Volume and Issue: 12(3), P. 553 - 553

Published: Jan. 26, 2023

The ascomycete Botrytis cinerea Pers. Fr., classified within the family Sclerotiniaceae, is agent that causes grey mould disease which infects at least 1400 plant species, including crops of economic importance such as grapes and strawberries. life cycle B. consists two phases: asexual (anamorph, Fr.) sexual (teleomorph, Botryotinia fuckeliana (de Bary) Wetzel). During XVI International Symposium dedicated to fungus, was held in Bari June 2013, scientific community unanimously decided assign most widely used name form, Botrytis, this genus fungi. However, literature, we continue find articles referring both morphic stages. In review, take stock genes metabolites reported for forms between January 2015 October 2022.

Language: Английский

Citations

18
Genetic and molecular landscapes of the generalist phytopathogen Botrytis cinerea DOI Creative Commons
Ritu Singh, Céline Caseys, Daniel J. Kliebenstein

et al.

Molecular Plant Pathology, Journal Year: 2023, Volume and Issue: 25(1)

Published: Dec. 1, 2023

Abstract Botrytis cinerea Pers. Fr. (teleomorph: Botryotinia fuckeliana ) is a necrotrophic fungal pathogen that attacks wide range of plants. This updated profile explores the extensive genetic diversity B. , highlights progress in genome sequencing, and provides current knowledge molecular mechanisms employed by fungus to attack its hosts. In addition, we also discuss recent innovative strategies combat . Taxonomy Kingdom: Fungi, phylum: Ascomycota, subphylum: Pezizomycotina, class: Leotiomycetes, order: Helotiales, family: Sclerotiniaceae, genus: species: Host infects almost all plant groups (angiosperms, gymnosperms, pteridophytes, bryophytes). To date, 1606 species have been identified as hosts Genetic polyphagous necrotroph has at population levels shaped climate, geography, host variation. Pathogenicity architecture virulence specificity polygenic using multiple weapons target hosts, including secretory proteins, complex signal transduction pathways, metabolites, mobile small RNA. Disease control Efforts being high‐diversity generalist pathogen, are complicated. However, integrated disease management combine cultural practices, chemical biological controls, use appropriate crop varieties will lessen yield losses. Recently, studies conducted worldwide explored potential RNA an efficient environmentally friendly approach for combating grey mould. additional research necessary, especially on risk assessment regulatory frameworks, fully harness this technology.

Language: Английский

Citations

18
Integration analysis of transcriptomics and proteomics reveals the inhibitory effect of carvacrol on Alternaria alternata by interfering with carbohydrate metabolism DOI
Lunaike Zhao, Junjie Wang, Haiyu Zhang

et al.

Postharvest Biology and Technology, Journal Year: 2024, Volume and Issue: 213, P. 112928 - 112928

Published: April 9, 2024

Language: Английский

Citations

7