Haplotype-resolved genome assembly provides insights into evolutionary history of the Actinidia arguta tetraploid

Molecular Horticulture, Journal Year: 2024, Volume and Issue: 4(1)

Published: Feb. 6, 2024

Actinidia arguta, known as hardy kiwifruit, is a widely cultivated species with distinct botanical characteristics such small and smooth-fruited, rich in beneficial nutrients, rapid softening tolerant to extremely low temperatures. It contains the most diverse ploidy types, including diploid, tetraploid, hexaploid, octoploid, decaploid. Here we report haplotype-resolved tetraploid genome (A. arguta cv. 'Longcheng No.2') containing four haplotypes, each 40,859, 41,377, 39,833 39,222 protein-coding genes. We described phased structure, synteny, evolutionary analyses identify date possible WGD events. K

Language: Английский

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Scientific and technological advances in the development of sustainable disease management tools: a case study on kiwifruit bacterial canker

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 11, 2024

Plant disease outbreaks are increasing in a world facing climate change and globalized markets, representing serious threat to food security. Kiwifruit Bacterial Canker (KBC), caused by the bacterium Pseudomonas syringae pv. actinidiae (Psa), was selected as case study for being an example of pandemic that severely impacted crop production, leading huge economic losses, effort has been made control this disease. This review provides in-depth critical analysis on scientific progress developing alternative tools sustainable KBC management. Their status terms technological maturity is discussed set opportunities threats also presented. The gradual replacement susceptible kiwifruit cultivars, with more tolerant ones, significantly reduced incidence major milestone Psa containment – which highlights importance plant breeding. Nonetheless, very laborious process. Moreover, potential evolving virulent biovars, or resistant lineages existing methods, strengthens need keep exploring effective environmentally friendly Currently, elicitors beneficial fungi bacteria already used field some degree success. Precision agriculture technologies, improving early detection preventing pathogen dispersal, developed optimized. These include hyperspectral technologies forecast models risk assessment, latter slightly advanced maturity. Additionally, protection products based innovative formulations molecules antibacterial activity against ( e.g ., essential oils, phages antimicrobial peptides) have validated primarily laboratory trials few compounds reaching application. lessons learned disease, acquired knowledge, can be sustainably managing other diseases handling future outbreaks.

Language: Английский

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Contrasting effector profiles between bacterial colonisers of kiwifruit reveal redundant roles converging on PTI‐suppression and RIN4

New Phytologist, Journal Year: 2023, Volume and Issue: 238(4), P. 1605 - 1619

Published: March 1, 2023

Testing effector knockout strains of the Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) for reduced in planta growth their native kiwifruit host revealed a number nonredundant effectors that contribute to Psa3 virulence. Conversely, complementation weak pathogen P. actinidifoliorum (Pfm) increased identified redundant effectors. hopAZ1a and HopS2b entire exchangeable locus (ΔEEL; 10 effectors) were significant contributors bacterial colonisation additive effects on Four EEL (HopD1a, AvrB2b, HopAW1a HopD2a) redundantly virulence through suppression pattern-triggered immunity (PTI). Important include several required early infection process (HopZ5a, HopH1a, AvrPto1b, AvrRpm1a HopF1e). These largely target plant hub, RIN4. This comprehensive profiling carries robust redundancy large portion its effectors, covering few functions critical disease.

Language: Английский

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Structural characterization, derivatization and antibacterial activity of secondary metabolites produced by termite‐associated Streptomyces showdoensisBYF17

Pest Management Science, Journal Year: 2023, Volume and Issue: 79(5), P. 1800 - 1808

Published: Jan. 14, 2023

Insect-associated Streptomyces is a valuable resource for development of compounds with antibacterial potential. However, relatively little known the secondary metabolites produced by termite-associated Streptomyces.Here, seven including o-acetaminophenol (1), phenazine-1,6-dicarboxylic acid (2), phenylacetic (3), phenazinolin D (4), izumiphenazine A (5), B (6) and E (7) were obtained from fermentation broth showdoensis BYF17, which was isolated body surfaces Odontotermes formosanus. Two additional novel derivative (6a 6b) synthesized via acetylation methylation, respectively. The structures these elucidated spectroscopic analyses. bioassay showed that compound 6a displayed strong inhibitory effects against Pseudomonas syringae pv. actinidiae (Psa), zone inhibition (ZOI) diameter 20.6 mm, comparable to positive gentamicin sulfate ZOI value 25.6 mm. Furthermore, Day 5 curative activities both 6 kiwifruit bacterial canker 71.5%, higher than those referred oxine-copper (55.0%) ethylicin (46.8%) at concentration 200 μg mL-1 . In addition, mechanism analysis based on scanning electron microscopic observation revealed destroyed integrity Psa cell membrane.The results biological tests bioactive exhibit potent antimicrobial activities, have potential be developed into new agents. © 2023 Society Chemical Industry.

Language: Английский

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The biological control agent for bacterial canker of kiwifruit, in Aureo® Gold, is a strain of Aureobasidium pullulans identifiable by novel SCAR marker primers

Biological Control, Journal Year: 2025, Volume and Issue: unknown, P. 105709 - 105709

Published: Jan. 1, 2025

Language: Английский

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Genomic Biosurveillance of the Kiwifruit Pathogen Pseudomonas syringae pv. actinidiae Biovar 3 Reveals Adaptation to Selective Pressures in New Zealand Orchards

Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: 26(2)

Published: Feb. 1, 2025

In the late 2000s, a pandemic of Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) devastated kiwifruit orchards growing susceptible, yellow-fleshed cultivars. New Zealand's industry has since recovered, following deployment tolerant cultivar 'Zesy002'. However, little is known about extent to which Psa population evolving its arrival. Over 500 Psa3 isolates from Zealand were sequenced between 2010 and 2022, commercial monocultures diverse germplasm collections. While effector loss was previously observed on Psa-resistant vines, appears be rare in orchards, where dominant cultivars lack resistance. new variant, lost hopF1c, arisen. The hopF1c have been mediated by movement integrative conjugative elements introducing copper resistance into this population. Following variant's identification, in-planta pathogenicity competitive fitness assays performed better understand risk likelihood spread. variants had similar growth wild-type Psa3, lab-generated ∆hopF1c strain could outcompete wild type select hosts. Further surveillance conducted these originally isolated, with 6.6% surveyed identified as variants. These findings suggest that spread currently limited, they are unlikely cause more severe symptoms than current Ongoing genome biosurveillance recommended enable early detection management interest.

Language: Английский

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Effector Repertoire of the Sweetpotato Black Rot Fungal Pathogen Ceratocystis fimbriata

Molecular Plant-Microbe Interactions, Journal Year: 2024, Volume and Issue: 37(3), P. 315 - 326

Published: Feb. 14, 2024

In 2015, sweetpotato producers in the United States experienced one of worst outbreaks black rot recorded history, with up to 60% losses reported field and packing houses at shipping ports. Host resistance remains ideal management tool decrease crop losses. Lack knowledge Ceratocystis fimbriata biology represents a critical barrier for deployment sweetpotato. this study, we scanned recent near chromosomal-level assembly putative secreted effectors C. isolate AS236 using custom fungal effector annotation pipeline. We identified set 188 on basis secretion signal silico prediction EffectorP. conducted deep RNA time-course sequencing experiment determine whether modulates planta define candidate list expressed during infection. examined expression profile two isolates, pre-epidemic (1990s) post-epidemic (2015) isolate. Our profiling revealed clusters co-expressed candidates. Based fold-change differences both isolates over course infection, suggested prioritization 31 functional characterization. Among set, several that provide evidence marked biotrophic phase infection storage roots. study catalog proteins insight into mechanisms represent core implement effector-assisted breeding [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under CC BY-NC-ND 4.0 International license .

Language: Английский

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Competition for nutrient niches within the apple blossom microbiota antagonizes the initiation of fire blight infection

New Phytologist, Journal Year: 2024, Volume and Issue: 243(6), P. 2385 - 2400

Published: July 19, 2024

Summary Changes in the plant microbiota composition are intimately associated with health of plant, but factors controlling microbial community flowers poorly understood. In this study, we used apple and fire blight as a model system to investigate effects floral competition on disease development suppression. To compare changes flora RNA expression patterns plants, flower samples were collected three different flowering stages (Bud, Popcorn, Full‐bloom). Using advanced sequencing technology, analyzed data conducted both vitro vivo experiments validate our findings. Our results show that Erwinia amylovora use arabinogalactan, which is secreted flowers, for early colonization flowers. Pantoea agglomerans was more competitive arabinogalactan than E. . Additionally, P suppressed virulence by using arabinose, major component induces gene expression. The present provide new insights into developing control strategies diverse diseases, including blight, highlighting importance nutrients or

Language: Английский

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Evolving Archetypes: Learning from Pathogen Emergence on a Nonmodel Host

Annual Review of Phytopathology, Journal Year: 2024, Volume and Issue: 62(1), P. 49 - 68

Published: June 17, 2024

Research initiatives undertaken in response to disease outbreaks accelerate our understanding of microbial evolution, mechanisms virulence and resistance, plant-pathogen coevolutionary interactions. The emergence global spread

Language: Английский

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Kiwifruit Resistance to Sclerotinia sclerotiorum and Pseudomonas syringae pv. actinidiae and Defence Induction by Acibenzolar-S-methyl and Methyl Jasmonate Are Cultivar Dependent

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(21), P. 15952 - 15952

Published: Nov. 3, 2023

Pathogen susceptibility and defence gene inducibility were compared between the Actinidia arguta cultivar ‘Hortgem Tahi’ two cultivars of A. chinensis ‘Hayward’ ‘Zesy002′. Plants treated with acibenzolar-s-methyl (ASM) or methyl jasmonate (MeJA) one week before inoculation Pseudomonas syringae pv. actinidiae (Psa biovar3) Sclerotinia sclerotiorum, secondary induction chitosan+glucan (Ch-Glu) as a potential pathogen proxy. Defence expression was evaluated by measuring 18 putative genes. highly susceptible to sclerotinia very resistant Psa, whereas ‘Zesy002′ both, moderately both. Gene in alike but differed significantly from which had higher basal levels PR1-i, PR5-i, JIH1, NPR3 WRKY70 lower RD22 PR2-i. Treatment ASM caused upregulation NIMIN2, WRKY70, DMR6 PR5-i all induced resistance Psa decreased MeJA application LOX2 downregulation PR2-i did not affect disease susceptibility. The Ch-Glu inducer PR-gene families each cultivar, highlighting its possible effectiveness an alternative actual inoculation. significance variations fundamental inducible among is explored.

Language: Английский

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