A viral movement protein targets host catalases for 26S proteasome-mediated degradation to facilitate viral infection and aphid transmission in wheat

Molecular Plant, Journal Year: 2024, Volume and Issue: 17(4), P. 614 - 630

Published: March 7, 2024

Language: Английский

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WRKY Transcription Factors (TFs) as Key Regulators of Plant Resilience to Environmental Stresses: Current Perspective

Agronomy, Journal Year: 2024, Volume and Issue: 14(10), P. 2421 - 2421

Published: Oct. 19, 2024

Plants encounter various stresses in their natural environments and can effectively respond to only one stress at a time. Through complex gene network, transcription factors (TFs) such as WRKY TFs regulate diverse array of responses. The clarification the structural characteristics proteins, along with recent advancements molecular dynamics simulations, has shed light on formation, stability, interactions DNA–protein complexes. This provided novel viewpoint regarding control TFs. investigation superfamilies, encompassing historical development, diversity, evolutionary patterns, become feasible due transcriptome approach’s capacity provide extensive comprehensive transcripts. significance lies pivotal role within several signaling cascades regulatory networks that influence plant defense present review summarizes functional aspects high-volume sequence data from different species studied date. Moreover, comparative analysis approach was utilized determine functions identified response both abiotic biotic stresses, revealed through numerous studies species. results this will be understanding events context climate change, incorporating new scientific evidence propose an innovative viewpoint.

Language: Английский

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Defense signaling pathways in resistance to plant viruses: Crosstalk and finger pointing

Advances in virus research, Journal Year: 2024, Volume and Issue: unknown, P. 77 - 212

Published: Jan. 1, 2024

Language: Английский

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Deciphering the role of WRKY transcription factors in plant resilience to alkaline salt stress

Plant Stress, Journal Year: 2024, Volume and Issue: 13, P. 100526 - 100526

Published: June 28, 2024

The WRKY gene family consists of unique transcription factors (TFs) found exclusively in plants. These TFs play a crucial role regulating how plants respond to various abiotic stresses, such as saline-alkaline conditions, temperature fluctuations, drought, UV radiation and others. Scientists have been progressively studying the roles mechanisms several plant species, including both model essential agricultural crops. This study focus has emerged due understanding that alkaline saline soil stressors considerably impede global productivity. Multiple research efforts underscored significant biological functions assisting coping with challenges, particularly enhancing their ability withstand alkaline-salt stress. review aims investigate structural capabilities impact on responses salt stresses. Additionally, it seeks elucidate these alleviating diverse biotic stressors. objective this is provide comprehensive insights into current state field importance

Language: Английский

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Evolution of Whirly1 in the angiosperms: sequence, splicing, and expression in a clade of early transitional mycoheterotrophic orchids

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: June 28, 2024

The plastid-targeted transcription factor Whirly1 ( WHY1 ) has been implicated in chloroplast biogenesis, plastid genome stability, and fungal defense response, which together represent characteristics of interest for the study autotrophic losses across angiosperms. While gene loss nuclear genomes well studied mycoheterotrophic plants, evolution molecular mechanisms impacting stability is completely unknown. Here, we characterize four early transitional orchid species genus Corallorhiza by synthesizing results phylogenetic, transcriptomic, comparative genomic analyses with sequences sampled from 21 orders We found an increased number non-canonical isoforms assembled all but greenest species, including intron retention some isoforms. Within , phylotranscriptomic revealed presence tissue-specific differential expression only most photosynthetically capable a coincident increase fully species. Gene- codon-level tests selective regimes did not infer significant signal either relaxed selection or episodic diversifying so late-stage full orchids Epipogium aphyllum Gastrodia elata . Additionally, nucleotide substitutions that likely impact function such as nonsense mutations, were observed mycoheterotrophs. propose our findings suggest splicing changes may precede shifts inferred therefore does support primary role transition to mycoheterotrophy Orchidaceae. Taken together, this provides comprehensive view angiosperms date.

Language: Английский

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Transcription Factors in Plant Biotic and Abiotic Stress Responses: Potentials and Prospects in Sugarcane

Tropical Plant Biology, Journal Year: 2025, Volume and Issue: 18(1)

Published: Jan. 31, 2025

Language: Английский

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Insights into geminiviral pathogenesis: interaction between βC1 protein and GLABROUS1 enhancer binding protein (GeBP) in Solanaceae

Phytopathology Research, Journal Year: 2025, Volume and Issue: 7(1)

Published: Feb. 10, 2025

Abstract Transcription factors (TFs) play crucial roles in plant development and pathogen defense. However, viruses can exploit TFs to facilitate their infection or transmission. In this study, we confirmed the βC1 proteins, encoded by tobacco curly shoot virus (TbCSV)- tomato yellow leaf curl China (TYLCCNV)-associated betasatellites, interacted with GLABROUS1 enhancer binding protein (GeBP) from solanaceous plants including Nicotiana benthamiana , Solanum lycopersicum S. tuberosum Capsicum annuum . Further analysis verified nuclear localization, homodimerization, DNA-binding ability of GeBP TFs, along its interaction nucleus. PVX-mediated overexpression NbGeBP showed no effect on accumulation viral betasatellite DNAs N. after TbCSV heterologous betasatellite, malvastrum vein associated (MaYVB), homologous TbCSB. both MaYVV caused a decrease expression during early stages infection, regardless presence implying that might role infection. TbCSV/TbCSB TYLCCNV/TYLCCNB infect many plants, proteins interact TbCSB TYLCCNB. The yeast two-hybrid bimoleccular fluorescence complementation assays AtGeBP Arabidopsis thaliana could not βC1, revealing GeBP-βC1 interactions only exist plants. Importantly, MaYVB, which was almost reported natural GeBP, suggesting potential monopartite begomovirus

Language: Английский

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Genome-wide identification and expression profiling of WRKY gene family in grain Amaranth (Amaranthus hypochondriacus L.) under salinity and drought stresses

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 28, 2025

The WRKY gene family plays a significant role in plant growth, development, and responses to biotic abiotic stresses. However, the of has not been reported Amaranthus hypochondriacus. This study presents comprehensive genome-wide analysis grain amaranth (A. hypochondriacus L.), resilient crop known for its high nutritional value adaptability challenging environments. In this study, 55 genes (AhyWRKY1-55) were identified A. distributed unevenly across 16 scaffolds. Of these, 50 contained conserved domains classified into three main groups. Group II was further divided five subgroups (IIa-IIe) based on phylogenetic analysis, with each clade being well supported by motifs. Additionally, structure revealed variations exon-intron organization. contrast, motif showed presence that similar within group but differed between groups, suggesting their functional diversity. Cis-acting elements related growth development light, hormones, stress identified. Synteny 34 (61.8%) originated from tandem duplication, indicating duplication expansion family. Protein-protein interaction suggested AhyWRKY3, AhyWRKY27, AhyWRKY28, AhyWRKY36, AhyWRKY52 hub involved complex protein network. Using silico real-time quantitative PCR, expression tissue- condition-specific patterns AhyWRKY genes. Notably, under drought stress, AhyWRKY39, AhyWRKY40, AhyWRKY54, AhyWRKY01 increased expression, while salt AhyWRKY49, AhyWRKY8 upregulated at 30 days, these may play key response salinity stress. present provides valuable insights organization evolutionary amaranth. It also identifies putative candidate conferring tolerance. Overall, lays foundation validation genes, facilitating exploitation genetic improvement programs develop stress-resilient varieties.

Language: Английский

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Identification of Interaction Between Geminivirus and Plant Proteins by Bimolecular Fluorescence Complementation (BiFC)

Methods in molecular biology, Journal Year: 2025, Volume and Issue: unknown, P. 227 - 234

Published: Jan. 1, 2025

Language: Английский

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Establishment of efficient Trichosanthes mottle mosaic virus-derived gene silencing in cucurbit plants

Stress Biology, Journal Year: 2025, Volume and Issue: 5(1)

Published: May 20, 2025

Abstract The Cucurbitaceae family includes a wide range of economically important fruits and vegetables; however, the laborious highly inefficient genetic transformation efficacy cucurbits has hindered exploration their gene functions. Virus-induced silencing (VIGS) technology, employed from antiviral RNA defense, emerged as viable alternative for high-throughput study plant function. In this study, we successfully established VIGS system utilizing Trichosanthes mottle mosaic virus (TrMMV), new member genus Tobamovirus . We demonstrated high durability mediated by TrMMV-VIGS vector in Nicotiana benthamiana , well several cucurbit species, including Cucurbita pepo Cucumis sativus C. lanatus melo insertion 90–400 bp fragments into led to effective target both with notably higher efficiency observed Furthermore, induced pronounced photobleaching phenotype flowers underscoring its potential application functional genomic research concerning floral traits particular species. Taken together, developed herein will facilitate rapid identification functions crops.

Language: Английский

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