Short-term exposure to interleukin-6 does not affect angiotensin-converting enzyme 2 expression in human pancreatic islets DOI Creative Commons
Anila Khalique, Mawieh Hamad, Jalal Taneera

et al.

Advances in Biomedical and Health Sciences, Journal Year: 2023, Volume and Issue: 2(4), P. 190 - 191

Published: Oct. 1, 2023

To the Editor, Previous studies have shown that individuals with diabetes exhibit compromised immunity, which contributes to their heightened susceptibility COVID-19 infection [1]. In Wuhan, approximately 50% of patients exhibited transient hyperglycemia, is partially attributed binding SARS-CoV-2 receptors on pancreatic islet cells [2,3]. The main cellular entry mechanism involves an interaction between viral spike S1 protein and angiotensin-converting enzyme 2 (ACE2) surface alveolar epithelial [4]. studies, including our own, reported cells, β-cells, express ACE2 [5,6], making them susceptible infection. This, in turn, results reduced insulin secretion capacity worsening symptoms. Patients severe experience, a cytokine storm syndrome, characterized by elevated interleukin (IL)-2, IL-7, granulocyte colony-stimulating factor, CXCL10, monocyte chemotactic 1, macrophage inflammatory protein-1α, tumor necrosis factor (TNF)-α, IL-6 levels [7,8]. This suggests disease severity can be infection-induced hyperinflammation, aggravates lung damage. pleiotropic exerts pro-inflammatory anti-inflammatory effects activating its different target [9]. Exposure human islets has been impair glucose-stimulated increase cell death [10,11]. implies (as observed COVID-19) may contribute impaired glucose metabolism development. contrast, other mediates α-cell mass proliferation protects β-cells from apoptosis [12,13]. However, impact expression remains unknown. study, were obtained three donors who nondiabetic tested negative for (purchased Prodo Lab. Inc., USA). These treated (200 ng/mL) 48 h, was subsequently assessed at both mRNA levels. As Figure 1a b, data indicated there no significant change IL-6-treated compared control islets.Figure 1: Impact treatment upon 200 ng/ml h. (a) Gene analysis as examined quantitative polymerase chain reaction (n = 3). (b) determined western blotting 2). ns: not significant. Bars represent mean ± standard deviation. IL: Interleukin, ACE: Angiotensin-converting enzyme.A previous report suggested exposure β-cell line "EndoC-βH1" palmitate does lead any alteration [6]. these mixture cytokines (IL-1 β, interferon-γ, TNFα) 24 h leads upregulation addition, identified interferon-stimulated gene various types [14,15]. best knowledge, this first study evaluate cells. Further investigation into tissues warranted. conclusion, provides additional insights relationship Authors' contributions JT proposed idea. JT, MH AK contributed discussion, reviewed edited letter. performed experiment analysed data. Data availability statement collected during work are available. Financial support sponsorship Not applicable. Conflict interests No conflict declared.

Language: Английский

Targeting extranuclear histones to alleviate acute and chronic inflammation DOI Creative Commons
Gerry A. F. Nicolaes, Oliver Soehnlein

Trends in Pharmacological Sciences, Journal Year: 2024, Volume and Issue: 45(7), P. 651 - 662

Published: June 12, 2024

Extracellular histones instigate an inflammatory triad - centered on cytotoxicity, immune cell stimulation, and coagulation ultimately shaping the dynamics outcome of various pathologies. Given virtual absence beneficial functions in extracellular space, recent years a number interference strategies have emerged. In this review we summarize pathogenic highlight current developments therapeutic interference. Finally, elaborate status preclinical attempts to interfere with context focus sepsis cardiovascular diseases, both which are leading causes mortality worldwide.

Language: Английский

Citations

7
Self-DNA driven inflammation in COVID-19 and after mRNA-based vaccination: lessons for non-COVID-19 pathologies DOI Creative Commons
Martin Heil

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 14

Published: Feb. 19, 2024

The coronavirus disease 2019 (COVID-19) pandemic triggered an unprecedented concentration of economic and research efforts to generate knowledge at unequalled speed on deregulated interferon type I signalling nuclear factor kappa light chain enhancer in B-cells (NF-κB)-driven interleukin (IL)-1β, IL-6, IL-18 secretion causing cytokine storms. translation the how resulting systemic inflammation can lead life-threatening complications into novel treatments vaccine technologies is underway. Nevertheless, previously existing role cytoplasmatic or circulating self-DNA as a pro-inflammatory damage-associated molecular pattern (DAMP) was largely ignored. Pathologies reported ‘ de novo ’ for patients infected with Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV)-2 be outcomes self-DNA-driven fact had been linked earlier different contexts, e.g., infection Human Immunodeficiency Virus (HIV)-1, sterile inflammation, autoimmune diseases. highlight particularly synergies other DAMPs render immunogenic properties normally non-immunogenic extracellular self-DNA, discuss shared features gp41 unit HIV-1 envelope protein SARS-CoV 2 Spike that enable SARS-CoV-2 interact cell membranes, trigger syncytia formation, inflict damage their host’s DNA, – likely own benefit. These similarities motivate speculations similar mechanisms those driven by explain inflammatory contributes some most frequent adverse events after vaccination BNT162b2 mRNA (Pfizer/BioNTech) mRNA-1273 (Moderna) vaccine, i.e., myocarditis, herpes zoster, rheumatoid arthritis, nephritis hepatitis, new-onset lupus erythematosus, flare-ups psoriasis lupus. hope wider application lessons learned from experiences COVID-19 new vaccines combat future non-COVID-19

Language: Английский

Citations

4
Monocyte distribution width (MDW) as a reliable diagnostic biomarker for sepsis in patients with HIV DOI Creative Commons
Jinfeng Sun, Yueming Shao, Rui Jiang

et al.

Emerging Microbes & Infections, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

Introduction: Sepsis is a leading cause of death among patients with HIV, but early diagnosis remains challenge. This study evaluates the diagnostic performance monocyte distribution width (MDW) in detecting sepsis HIV.

Language: Английский

Citations

0
Monocyte distribution width as an early predictor of short-term outcome in adult patients with sepsis DOI
Yin Liu, Ruizhi Wang,

Shihua Zhong

et al.

Clinical Chemistry and Laboratory Medicine (CCLM), Journal Year: 2023, Volume and Issue: 62(3), P. 562 - 571

Published: Oct. 10, 2023

Monocyte distribution width (MDW) is a quantitative measurement of monocyte anisocytosis and has been proposed as an efficient marker for early sepsis detection. This study aimed to assess the prognostic potential MDW in septic patients.

Language: Английский

Citations

8
Thrombocytopenia and hyperinflammation are induced by extracellular histones circulating in blood DOI
Rosaria Vincenza Giglio, Daniela Ligi,

Chiara Della Franca

et al.

Clinical Chemistry and Laboratory Medicine (CCLM), Journal Year: 2023, Volume and Issue: 61(12), P. e239 - e243

Published: June 20, 2023

Language: Английский

Citations

4
Novel inflammatory biomarkers in the prognosis of COVID-19 DOI Creative Commons

Kegang Zhan,

Luhan Wang, Hao Lin

et al.

Therapeutic Advances in Respiratory Disease, Journal Year: 2023, Volume and Issue: 17

Published: Jan. 1, 2023

The central role of inflammatory progression in the development Coronavirus disease 2019 (COVID-19), especially severe cases, is indisputable. However, some novel biomarkers prognosis COVID-19 remains controversial.

Language: Английский

Citations

4
Current issues of post-covid syndrome consequences DOI Creative Commons
M.V. Krasnoselskyi,

V.P. Starenkyі,

N.O. Artamonova

et al.

Український радіологічний та онкологічний журнал, Journal Year: 2023, Volume and Issue: 31(3), P. 325 - 352

Published: Sept. 30, 2023

Background. The World Health Organization (WHO) has confirmed the outbreak of covid infection and defined it as a global pandemic. Recently, complications have been increasingly reported in patients who had COVID-19. At same time, clinical symptoms long-term course this disease overlap, differing from acute manifestations infection, may persist recovered for several weeks, months or years, adding number other problems. Therefore, importance thorough study post-COVID-19 syndrome modern phenomenon increased. And requires proper research to improve efficiency its prevention treatment. Purpose. To evaluate current state problem syndrome, particular cancer patients. Materials methods. A literature review was performed based on array digital publications found world databases PubMed, Scopus Web Science Core Collection 2020–2023. sources were systematized separate groups, i.e., issues related post-Covid, particular, mechanisms pathogenesis development; treatment features, including patients; consequences patients, etc. Results discussion. terminology subject analyzed: post-COVID, post-COVID PASC, long-COVID, COVID, chronic etc. views regarding main systematized. It is determined that field oncology, conducted certain fields risk progression occurrence period evaluated; process care organization level mortality suffered pandemic also led multiple changes radiological diagnosis with COVID. Evaluating impact outcomes adaptation radiotherapy chemotherapy, delays provision timely regimens will affect survival Conclusion. Assessing most researchers believe during delay negatively coincidence cancer-related COVID another major challenge oncologists future.

Language: Английский

Citations

2
The Need for Standardized Guidelines for the Use of Monocyte Distribution Width (MDW) in the Early Diagnosis of Sepsis DOI Open Access
Andrea Piccioni,

Fabio Spagnuolo,

Silvia Baroni

et al.

Journal of Personalized Medicine, Journal Year: 2024, Volume and Issue: 15(1), P. 5 - 5

Published: Dec. 27, 2024

Sepsis is a complex and potentially life-threatening syndrome characterized by an abnormal immune response to infection, which can lead organ dysfunction, septic shock, death. Early diagnosis crucial improving prognosis reducing hospital management costs. This narrative review aims summarize evaluate the current literature on role of monocyte distribution width (MDW) as diagnostic biomarker for sepsis, highlighting its advantages, limitations, potential clinical applications. MDW measures volumetric monocytes, reflecting monocytic anisocytosis, detected using advanced hematological analyzers. In 2019, it was approved FDA sepsis due ability identify systemic inflammatory at early stage. Thirty-one studies analyzed us have shown that increased value associated with higher risk combination parameters (such qSOFA) other biomarkers (CRP, PCT) enhance sensitivity stratification capacity. Despite high sensitivity, has lower specificity compared more established such procalcitonin, thus requiring multimodal integration accurate diagnosis. The use in emergency intensive care settings represents opportunity improve critical patient management, particularly when combined markers tools. However, further are needed define universal cut-off confirm validity different contexts pathological scenarios.

Language: Английский

Citations

0
Novel mechanisms of thrombo-inflammation during infection: the harmful impact of circulating histones DOI Creative Commons
Daniela Ligi,

Chiara Della Franca,

Ferdinando Mannello

et al.

Research and Practice in Thrombosis and Haemostasis, Journal Year: 2023, Volume and Issue: 7(3), P. 100141 - 100141

Published: March 1, 2023

We read with great interest the exhaustive review/state of art by Colicchia et al. [[1]Colicchia M. Perrella G. Gant P. Rayes J. Novel mechanisms thrombo-inflammation during infection: spotlight on neutrophil extracellular trap-mediated platelet activation.Res Pract Thromb Haemost. 2023; 7100116Abstract Full Text PDF PubMed Scopus (3) Google Scholar], in which new data were shown through coordinate activation platelets and neutrophils bacterial viral sepsis. appreciate their careful review, we wish to comment some associated issues for integrating updating readers about biological biochemical knowledge related harmful effects circulating histones. Ever increasingly important evidence highlights that histones (intranuclear positively charged proteins, chromatin) show extranuclear/extracellular functions when extruded mainly activated white blood cells infections [[2]Li X. Ye Y. Peng K. Zeng Z. Chen L. Histones: critical players innate immunity.Front Immunol. 2022; 131030610Google Scholar,[3]De Vries F. Huckriede Wichapong Reutelingsperger C. Nicolaes G.A.F. The role COVID-19.J Intern Med. 293: 275-292Crossref (4) Scholar]. In this respect, have been demonstrated as inducers cellular damage cytotoxic proinflammatory effects, promoting monocyte aggregation, well finally acting damage-associated molecular pattern molecules A growing number recent studies underlined involvement inflammatory processes (eg, COVID-19-associated sepsis), shedding light roles both triggers potential therapeutic targets [[3]De Recently, increased levels also correlated worsening several diseases, raising usefulness stratify patients at higher risk morbidity/mortality Other than monocytes are well-recognized key immunity against pathogens Scholar]; is characterized peculiar morphologic changes, mirroring cell heterogeneity anisocytosis, clinically quantified distribution width (MDW) test, a mathematical parameter based measurement volume (by direct current impedance), conductivity radio frequency opacity), scatter laser beam intracellular/intranuclear scatter) population [[4]Ligi D. Lo Sasso B. Giglio R.V. Maniscalco R. DellaFranca Agnello al.Circulating contribute MDW alterations common mediators classical COVID-19 sepsis.Crit Care. 26: 260Crossref (9) When overreact (as infection or injury sepsis) they kick up cytokine storm [[5]Ligi Della Franca Ciaccio al.Monocyte modulated histones.Clin Chem Lab https://doi.org/10.1515/cclm-2023-0093Crossref (2) hyperinflammatory reaction inducing vicious cycle leading severe/critical conditions up-to death recently trigger changes responses anisocytosis those observed sepsis Moreover, determined alteration indices count, mean volume; [PDW], cytokines [eg, Platelet-derived growth factor-BB, Regulated Activation, Normal T Expressed Secreted, transforming factor beta-1, beta-2 beta-3]), revealing may significantly thrombocytopenia According outstanding focus neutrophil-mediated crucial interpretation novel considering emerging hyperinflammation (determined PDW), would reinforce integrate conclusions suggesting evaluation an index heterogenous populations), PDW activated, aggregated and/or ballooning/giant platelets), histone concentrations provide possible future tool timely predict worst outcome classic None declared.

Language: Английский

Citations

0
Short-term exposure to interleukin-6 does not affect angiotensin-converting enzyme 2 expression in human pancreatic islets DOI Creative Commons
Anila Khalique, Mawieh Hamad, Jalal Taneera

et al.

Advances in Biomedical and Health Sciences, Journal Year: 2023, Volume and Issue: 2(4), P. 190 - 191

Published: Oct. 1, 2023

To the Editor, Previous studies have shown that individuals with diabetes exhibit compromised immunity, which contributes to their heightened susceptibility COVID-19 infection [1]. In Wuhan, approximately 50% of patients exhibited transient hyperglycemia, is partially attributed binding SARS-CoV-2 receptors on pancreatic islet cells [2,3]. The main cellular entry mechanism involves an interaction between viral spike S1 protein and angiotensin-converting enzyme 2 (ACE2) surface alveolar epithelial [4]. studies, including our own, reported cells, β-cells, express ACE2 [5,6], making them susceptible infection. This, in turn, results reduced insulin secretion capacity worsening symptoms. Patients severe experience, a cytokine storm syndrome, characterized by elevated interleukin (IL)-2, IL-7, granulocyte colony-stimulating factor, CXCL10, monocyte chemotactic 1, macrophage inflammatory protein-1α, tumor necrosis factor (TNF)-α, IL-6 levels [7,8]. This suggests disease severity can be infection-induced hyperinflammation, aggravates lung damage. pleiotropic exerts pro-inflammatory anti-inflammatory effects activating its different target [9]. Exposure human islets has been impair glucose-stimulated increase cell death [10,11]. implies (as observed COVID-19) may contribute impaired glucose metabolism development. contrast, other mediates α-cell mass proliferation protects β-cells from apoptosis [12,13]. However, impact expression remains unknown. study, were obtained three donors who nondiabetic tested negative for (purchased Prodo Lab. Inc., USA). These treated (200 ng/mL) 48 h, was subsequently assessed at both mRNA levels. As Figure 1a b, data indicated there no significant change IL-6-treated compared control islets.Figure 1: Impact treatment upon 200 ng/ml h. (a) Gene analysis as examined quantitative polymerase chain reaction (n = 3). (b) determined western blotting 2). ns: not significant. Bars represent mean ± standard deviation. IL: Interleukin, ACE: Angiotensin-converting enzyme.A previous report suggested exposure β-cell line "EndoC-βH1" palmitate does lead any alteration [6]. these mixture cytokines (IL-1 β, interferon-γ, TNFα) 24 h leads upregulation addition, identified interferon-stimulated gene various types [14,15]. best knowledge, this first study evaluate cells. Further investigation into tissues warranted. conclusion, provides additional insights relationship Authors' contributions JT proposed idea. JT, MH AK contributed discussion, reviewed edited letter. performed experiment analysed data. Data availability statement collected during work are available. Financial support sponsorship Not applicable. Conflict interests No conflict declared.

Language: Английский

Citations

0