Osteogenesis capability of three-dimensionally printed poly(lactic acid)-halloysite nanotube scaffolds containing strontium ranelate

Nanotechnology Reviews, Journal Year: 2022, Volume and Issue: 11(1), P. 1901 - 1910

Published: Jan. 1, 2022

Abstract In this study, three-dimensional (3D) printing of 3D scaffolds containing halloysite nanotubes (HNTs) and strontium ranelate (SrR) as a carrier for the promotion bone regeneration is investigated. SrR acts an anabolic bone-forming anti-catabolic agent, while HNTs act SrR. Poly(lactic acid) (PLA) used biodegradable matrix The effects addition on morphological, biological, in vitro release properties are evaluated. morphological results show homogeneous structure with proper pore size (approximately 400 µm) suitable osteogenesis. contact angle decreased after to scaffold 67.99°, cell attachment. X-ray diffraction shows that homogenously molecularly distributed PLA reduces crystallinity prepared scaffolds. demonstrate profile stable, relatively linear, continuous within 21 days (504 h). A cumulative approximately 49% obtained controlled 504 h (21 days) low primary burst (12%). Human adipose stem cells cultured 3D-printed can efficiently promote biocompatibility, alkaline phosphatase activity, alizarin red staining.

Language: Английский

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Novel Approaches and Biomaterials for Bone Tissue Engineering: A Focus on Silk Fibroin

Materials, Journal Year: 2022, Volume and Issue: 15(19), P. 6952 - 6952

Published: Oct. 7, 2022

Bone tissue engineering (BTE) represents a multidisciplinary research field involving many aspects of biology, engineering, material science, clinical medicine and genetics to create biological substitutes promote bone regeneration. The definition the most appropriate biomaterials structures for BTE is still challenge researchers, aiming at simultaneously combining different features such as generation properties, biocompatibility, porosity mechanical strength. In this scenario, among BTE, silk fibroin valuable option development functional devices because its unique properties multiple chances processing. This review article aims providing reader with general overview recent progresses in terms approaches materials special focus on related mechanisms involved regeneration, presenting interesting results obtained by groups, which assessed great potential protein engineering.

Language: Английский

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Technological advances in three-dimensional skin tissue engineering

REVIEWS ON ADVANCED MATERIALS SCIENCE, Journal Year: 2023, Volume and Issue: 62(1)

Published: Jan. 1, 2023

Abstract Tissue engineering is an enabling technology that can be used to repair, replace, and regenerate different types of biological tissues holds great potential in various biomedical applications. As the first line defense for human body, skin has a complex structure. When injured by trauma or disease, may under natural conditions, though often resulting irreversible aesthetically unpleasant scarring. The development tissue strategies was reviewed. Although traditional approaches have made good progress, they are still unable effectively deal with large-area injuries produce full-thickness grafts. In vitro three-dimensional (3D) constructs equivalent substitutes promoted many major innovative discoveries biology medicine. 3D manufacturing divided into two categories: scaffold-free scaffold-based. representatives transwell/Boyden chamber approach organotypic culture. Because its low cost high repeatability, model currently commonly cytotoxicity analysis, cell biochemical high-throughput function. At present, drug experiments use artificial developed replace animal models. bioprinting scaffold-based approach. novel technology, it quickly design build multi-functional model. This offers new opportunities organs layer layer, now regenerative medicine meet increasing need suitable transplantation. generate improved quality complexity wound healing disease modeling. this review, we analyze conventional techniques engineer compare them bioprinting. We also summarized equipment, bioinks, scaffolds engineering. these culture techniques, focus on technology. While maturing improvements protocols required, promise skin-related

Language: Английский

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A review of glycosaminoglycan-modified electrically conductive polymers for biomedical applications

Acta Biomaterialia, Journal Year: 2023, Volume and Issue: 169, P. 45 - 65

Published: July 31, 2023

The application areas of electrically conductive polymers have been steadily growing since their discovery in the late 1970s. Recently, found way into biomedicine, allowing realization many relevant applications ranging from bioelectronics to scaffolds for tissue engineering. Extracellular matrix components, such as glycosaminoglycans, build an important class biomaterials that are heavily researched biomedical due favorable properties. Due highly anionic character and presence sulfate groups these biomolecules can be employed functionalize polymers, which enables tailorability improvement cell-material interactions polymers. This review paper gives overview recent research on glycosaminoglycan-modified intended discusses effect different biological dopants material characteristics, surface roughness, stiffness, electrochemical Moreover, key findings characterization vitro vivo summarized, remaining challenges field, particularly related modification with glycosaminoglycans achieve improved functional outcomes, discussed. development based (CPs) various applications, neural regeneration, drug delivery, or bioelectronics, has increasingly investigated over last decades. Recent literature shown changes synthesis procedure chosen dopant could adjust resulting characteristics. Hence, interesting approach lies using natural CPs tailor outcome. comprehensively summarizes state art field first time, highlighting morphology properties, consequently, interactions.

Language: Английский

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Recent advances in biofabrication strategies based on bioprinting for vascularized tissue repair and regeneration

Materials & Design, Journal Year: 2023, Volume and Issue: 229, P. 111885 - 111885

Published: March 31, 2023

Vascularization plays a crucial role in transporting and exchanging nutrients oxygen between implanted grafts with the host tissue. In biofabrication of grafts, remodeling vascular networks can accelerate vascularized tissue repair regeneration. Given heterogeneity tissues, traditional scaffold manufacturing techniques cannot effectively achieve various scales vitro vivo biomimetic. recent years, 3D bioprinting technologies have been widely used fabricating for regeneration due to their shape customizability, simple procedure, reproducibility, precise multi-dimensional control. With rapid development technologies, bioprinting-based strategies gradually applied construction tissues. Based on this background, our study aimed review advances, challenges, future perspectives based The techniques, bioinks, seed cells, growth factors were also enrolled review. addition, history, vessel formation mechanism, histology discussed.

Language: Английский

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