Cilostazol is a promising anti-pseudomonal virulence drug by disruption of quorum sensing

AMB Express, Journal Year: 2024, Volume and Issue: 14(1)

Published: Aug. 1, 2024

Abstract Resistance to antibiotics is a critical growing public health problem that desires urgent action combat. To avoid the stress on bacterial growth evokes resistance development, anti-virulence agents can be an attractive strategy as they do not target growth. Quorum sensing (QS) systems play main roles in controlling production of diverse virulence factors and biofilm formation bacteria. Thus, interfering with QS could result mitigation virulence. Cilostazol antiplatelet vasodilator FDA approved drug. This study aimed evaluate activities cilostazol light its possible interference Pseudomonas aeruginosa . Additionally, examines cilostazol’s impact bacterium’s ability induce infection vivo, using sub-inhibitory concentrations minimize risk development. In this context, formation, influence vivo were assessed presence at concentration. Furthermore, outcome combination was evaluated. interfered P. Moreover, swarming motility, significantly diminished. Histopathological investigation revealed liver, spleen kidney tissues damage abolished mice injected cilostazol-treated exhibited synergistic when used antibiotics. At molecular level, downregulated genes showed considerable affinity receptors. conclusion, adjunct therapy for treating Pseudomonal infections. research highlights potential combat infections by targeting mechanisms, reducing antibiotic resistance, enhancing treatment efficacy against These findings open avenues repurposing existing drugs, offering new, safer, more effective control strategies.

Language: Английский

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Bacteria-Inspired Synthesis of Silver-Doped Zinc Oxide Nanocomposites: A Novel Synergistic Approach in Controlling Biofilm and Quorum-Sensing-Regulated Virulence Factors in Pseudomonas aeruginosa

Antibiotics, Journal Year: 2025, Volume and Issue: 14(1), P. 59 - 59

Published: Jan. 9, 2025

Multidrug-resistant Pseudomonas aeruginosa infections pose a critical challenge to healthcare systems, particularly in nosocomial settings. This drug-resistant bacterium forms biofilms and produces an array of virulent factors regulated by quorum sensing. In this study, metal-tolerant bacteria were isolated from metal-contaminated site screened for their ability synthesize multifunctional nanocomposites (NCs). Rapid color changes the reaction solution evidenced biotransformation process. The potent Bacillus cereus SASAK, identified via 16S rRNA sequencing deposited GenBank under accession number MH885570, facilitated microbial-mediated synthesis ZnO nanoparticles silver-doped NCs. These biogenic characterized using UV-VIS-NIR spectroscopy, FTIR, XRD, zeta potential, HRTEM, FESEM, EDX analyses. At sub-MIC concentration 100 µg/mL, 2% Ag-ZnO NCs effectively inhibited factor production biofilm formation P. without affecting bacterial growth. Notably, there was significant reduction violacein pigment (96.25%), swarming motility, pyocyanin (1.87 µg/mL). Additionally, (81.1%) EPS (83.9%) substantially hindered, along with reduced extracellular protease activity, as indicated zone (from 2.3 1.8 cm). study underscores potential promising agents combating sensing-mediated virulence chronic caused multidrug-resistant aeruginosa.

Language: Английский

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Sulforaphane Is Not Only a Food Supplement: It Diminishes the Intracellular Survival and Colonization of Salmonella enterica

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 16, 2025

Sulforaphane is a main bioactive component in several edible cruciferous vegetables. It acquires benefits to health addition its considered antibacterial and antivirulence activities. Herein, we aimed at evaluating the activity of sulforaphane against worldwide clinically important enteric pathogen Salmonella enterica serovar Typhimurium. The influence on bacterial adhesion, invasion, biofilm formation, intracellular replication was assayed. Additionally, effect type III secretion system (TTSS) S. quantified. outcome combination with different antibiotics assessed, an vivo protection assay conducted assess pathogenesis. results showed significant antibiofilm subinhibitory reduction invasion inside host cells. findings emphasized decreased capacity induce pathogenesis presence sulforaphane. Our finding attributed these activities interference TTSS-type II downregulation encoding genes. In nutshell, vegetable safe adjunct therapy that can be administrated alongside traditional for treating pathogens as enterica.

Language: Английский

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Bacterial Metabolites in Attack

Published: Jan. 1, 2025

Language: Английский

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Eco-friendly nano colloids for enhanced black gram (Vigna mungo) seed viability: experimental and computational analysis

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 15, 2025

An experiment was designed to fabricate Polyvinylpyrrolidone-coated zein-zipped herbal molecules infused nano colloids (PZCA-NCs) for extending Vigna mungo seeds storability. PZCA-NCs synthesized and characterized in Fourier Transform Infrared Spectroscopy (FTIR), X-Ray diffraction (XRD), Particle size analyser, Zeta Potential, Scanning Electron Microscopy (SEM), Transmission (TEM), Energy-dispersive X-ray spectroscopy (EDAX). The bio-efficacy of on seed storability tested under accelerated ageing. sphere-shaped possess a 151 nm with 44.5mV zeta potential at an encapsulation 73.44% curcumin 69.0% azadirachtin. spectra FTIR, UV -Vis, XRD, TGA confirmed the functionality, composition, stability PZCA-NCs. dialysis diffusion method utilised study maximum cumulative release biomolecules 6.1ppm (88.4%) azadirachtin 64.57ppm (88.2%) pH 7.4. Density functional theory (DFT) used determine binding mode examine ligand interactions treated 25 mL/kg enumerated higher germination, vigour index, α-amylase, dehydrogenase, catalase peroxidase activity Seeds storage pathogen infection reduced increase concentration coating. bioassay results insect evidenced that 15.76 killed 50% 40 100% Callosobruchus maculatus. Toxicity Macrophomina phaseolina showed 35 mL resulted 0.8 cm mycelia growth 91.11% inhibition zone, while 45 had zero fungal inhibition. concludes act as efficient invigoration material extend vitality during

Language: Английский

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Reprofiling lamivudine as an antibiofilm and anti-pathogenic agent against Pseudomonas aeruginosa

AMB Express, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 22, 2025

Resistance to antibiotics is a critical growing public health problem that needs urgent action combat. To avoid the stress on bacterial growth evokes development of resistance, anti-virulence agents can be an attractive strategy as they do not target growth. There are FDA approved drugs have been screened for their activities. Lamivudine (LAM) synthetic nucleoside analogue used antiretroviral in treatment HIV and HBV. The present study aimed assess activities LAM against clinically important pathogen Pseudomonas aeruginosa. LAM's antibiofilm were evaluated. impact quorum sensing (QS) systems which control production these virulence factors was assessed virtually by quantification expression QS-encoding genes. Furthermore, vivo mice protection assay conducted attest anti-pathogenic activity. current findings elaborated promising anti-QS LAM. interfered with biofilm formation P. aeruginosa PAO1 strain. Moreover, swarming motility pyocyanin protease significantly diminished. At molecular level, downregulated genes LasI, LasR, RhlR, PqsA PqsR. Additionally, detailed silico docking simulation studies showed considered high ability bind hinder QS receptors In agreement vitro silico, results full conclusion, could repurposed employed adjunct therapy traditional treating serious pseudomonal infections.

Language: Английский

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Enoxacin adversely affects Salmonella enterica virulence and host pathogenesis through interference with type III secretion system type II (T3SS-II) and disruption of translocation of Salmonella Pathogenicity Island-2 (SPI2) effectors

The Journal of Microbiology, Journal Year: 2025, Volume and Issue: 63(2), P. e2410015 - e2410015

Published: Feb. 27, 2025

Language: Английский

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Honokiol from Magnolia Tree Exhibits Antibacterial and Anti-virulence Potential against Pseudomonas aeruginosa

Arabian Journal for Science and Engineering, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 8, 2024

Language: Английский

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Eco-friendly Nano Colloids for Enhanced Black gram (Vigna mungo) Seed Viability: Experimental and Computational Analysis

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 17, 2024

An experiment was designed to fabricate Polyvinylpyrrolidone-coated zein-zipped herbal molecules infused nano colloids (PZCA-NCs) for extending Vigna mungo seeds storability. PZCA-NCs synthesized and characterized in Fourier Transform Infrared Spectroscopy (FTIR), X-Ray diffraction (XRD), Particle size analyser, Zeta Potential, Scanning Electron Microscopy (SEM), Transmission (TEM), Energy-dispersive X-ray spectroscopy (EDAX). The bio-efficacy of on seed storability tested under accelerated ageing. sphere-shaped possess a 151nm with 44.5mV zeta potential at an encapsulation 73.44 % curcumin 69.0 azadirachtin. spectra FTIR, UV –Vis, XRD, TGA confirmed the functionality, composition, stability PZCA-NCs. dialysis diffusion method utilised study maximum cumulative release biomolecules 6.1ppm (88.4%) azadirachtin 64.57ppm (88.2%) pH 7.4. Density functional theory (DFT) used determine binding mode examine ligand interactions treated seeds 25mL/kg enumerated higher germination, vigour index, α-amylase, dehydrogenase, catalase peroxidase activity Seeds storage pathogen infection reduced increase concentration coating. bioassay results insect evidenced that 15.76mL/kg killed 50% 40mL/kg 100% Callosobruchus maculatus. Toxicity Macrophomina phaseolina showed 35mL resulted 0.8cm mycelia growth 91.11% inhibition zone, while 45mL had zero fugal 100 inhibition. concludes act as efficient invigoration material extend vitality mungo during

Language: Английский

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Antimicrobial Nanoparticles Composed of Zein and Arginine-Phenylalanine-Based Surfactants for Wound Related Infections: Antioxidant and Skin-Related Anti-Enzymatic Activities and Toxicity

Antibiotics, Journal Year: 2024, Volume and Issue: 13(12), P. 1149 - 1149

Published: Dec. 1, 2024

Background/Objectives: Cationic surfactants are potential antimicrobial candidates. Even so, they the foremost irritative and incompatible group, which limits their usage. The incorporation of in biopolymer-based nanoparticles is a feasible strategy to improve efficacy reduce those drawbacks. Methods: Surfactants with one amino acid on polar head (lauroyl arginine methyl ester—LAM phenylalanine dodecyl amide—PNHC12) two acids heads, arginine-phenylalanine (Lauroyl esther—C12PAM phenylalanine-arginine amide—PANHC12) were loaded zein nanoparticles. Their antibiofilm activities evaluated. Also, inhibitory over skin-related enzymes accessed silico vitro, while cytotoxicity was determined comparatively immortal human keratinocytes (HaCaT) fibroblasts (3T3). Finally, Vibrio fisheri luminescence reduction test used detect its ecotoxicity. Results: obtained successfully exhibited good biocide activity against wide range pathogenic bacteria yeasts. found active assayed: elastase > tyrosinase collagenase lipoxygenase, superior when nanoencapsulated lipoxygenase. corresponding presented acceptable cytotoxic levels, except for PNHC12 both forms, ecotoxicity limited acceptable. Conclusions: Accordingly, nanoencapsulation be smart enhance selectivity representative skin connective tissues cell lines. These biological properties render surfactant as promising candidates tissue repairing applications wound treatments.

Language: Английский

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