Three Small-Receptive-Field Ganglion Cells in the Mouse Retina Are Distinctly Tuned to Size, Speed, and Object Motion DOI Creative Commons
Jason Jacoby, Gregory W. Schwartz

Journal of Neuroscience, Journal Year: 2016, Volume and Issue: 37(3), P. 610 - 625

Published: Dec. 5, 2016

Retinal ganglion cells (RGCs) are frequently divided into functional types by their ability to extract and relay specific features from a visual scene, such as the capacity discern local or global motion, direction of stimulus orientation, contrast uniformity, presence large small objects. Here we introduce three previously uncharacterized, nondirection-selective ON–OFF RGC that represent distinct set feature detectors in mouse retina. The high-definition (HD) RGCs possess receptive-field centers strong surround suppression. They respond selectively objects sizes, speeds, motion. We present comprehensive morphological characterization HD physiological recordings light responses, size structure, synaptic mechanisms also explore similarities differences between well characterized with comparably receptive field, edge detector, response moving textures. model populations each type study how they differ performance tracking object. These results, besides introducing new together constitute substantial fraction RGCs, provide insights role different circuits shaping fields establish foundation for continued suppression neural basis motion detection. SIGNIFICANCE STATEMENT output retina, retinal (RGCs), diverse group ∼40 neuron often assigned “feature detection” profiles based on aspects scene which respond. describe, first time, substantially augmenting our understanding selectivity. Experiments modeling show while these “high-definition” share certain properties, have tuning size, speed, enabling them occupy niches space.

Language: Английский

Unified classification of mouse retinal ganglion cells using function, morphology, and gene expression DOI Creative Commons

Jillian J. Goetz,

Zachary F. Jessen,

Anne Jacobi

et al.

Cell Reports, Journal Year: 2022, Volume and Issue: 40(2), P. 111040 - 111040

Published: July 1, 2022

Classification and characterization of neuronal types are critical for understanding their function dysfunction. Neuronal classification schemes typically rely on measurements electrophysiological, morphological, molecular features, but aligning such datasets has been challenging. Here, we present a unified mouse retinal ganglion cells (RGCs), the sole output neurons. We use visually evoked responses to classify 1,859 RGCs into 42 types. also obtain morphological or transcriptomic data from subsets these align functional publicly available datasets. create an online database that allows users browse download light using machine learning algorithm. This work provides resource studies RGCs, upstream circuits in retina, projections brain, establishes framework future efforts open distribution.

Language: Английский

Citations

168
Neuron-glia signaling in developing retina mediated by neurotransmitter spillover DOI Creative Commons
Juliana M. Rosa, Rémi Bos, Georgeann S. Sack

et al.

eLife, Journal Year: 2015, Volume and Issue: 4

Published: Aug. 14, 2015

Neuron-glia interactions play a critical role in the maturation of neural circuits; however, little is known about pathways that mediate their communication developing CNS. We investigated neuron-glia signaling retina, where we demonstrate retinal waves reliably induce calcium transients Müller glial cells (MCs). During cholinergic waves, MC were blocked by muscarinic acetylcholine receptor antagonists, whereas during glutamatergic inhibited ionotropic glutamate indicating responsiveness MCs changes to match neurotransmitter used support waves. Using an optical sensor show decline caused reduction amount reaching MCs. Together, these studies indicate neurons and exhibit correlated activity period enabled spillover from synapses.

Language: Английский

Citations

167
Different Modes of Visual Integration in the Lateral Geniculate Nucleus Revealed by Single-Cell-Initiated Transsynaptic Tracing DOI Creative Commons
Santiago B. Rompani, Fiona E. Müllner, Adrian Wanner

et al.

Neuron, Journal Year: 2017, Volume and Issue: 93(4), P. 767 - 776.e6

Published: Feb. 1, 2017

The thalamus receives sensory input from different circuits in the periphery. How these channels are integrated at level of single thalamic cells is not well understood. We performed targeted single-cell-initiated transsynaptic tracing to label retinal ganglion that provide individual principal mouse lateral geniculate nucleus (LGN). identified three modes integration by LGN cells. In first, 1-5 mostly same type converged one eye, indicating a relay mode. second, 6-36 types revealing combination third, up 91 both eyes, binocular mode which functionally specialized ipsilateral inputs joined broadly distributed contralateral inputs. Thus, employs least visual integration, each exhibiting degrees specialization.

Language: Английский

Citations

154
The First Stage of Cardinal Direction Selectivity Is Localized to the Dendrites of Retinal Ganglion Cells DOI Creative Commons
Keisuke Yonehara, Karl Farrow, Alexander Ghanem

et al.

Neuron, Journal Year: 2013, Volume and Issue: 79(6), P. 1078 - 1085

Published: Aug. 22, 2013

Language: Английский

Citations

143
Analogous Convergence of Sustained and Transient Inputs in Parallel On and Off Pathways for Retinal Motion Computation DOI Creative Commons

Matthew J. Greene,

Jinseop S. Kim, H. Sebastian Seung

et al.

Cell Reports, Journal Year: 2016, Volume and Issue: 14(8), P. 1892 - 1900

Published: Feb. 20, 2016

Language: Английский

Citations

131
Glutamate Clearance Is Locally Modulated by Presynaptic Neuronal Activity in the Cerebral Cortex DOI Creative Commons
Moritz Armbruster,

Elizabeth Hanson,

Chris G. Dulla

et al.

Journal of Neuroscience, Journal Year: 2016, Volume and Issue: 36(40), P. 10404 - 10415

Published: Oct. 5, 2016

Excitatory amino acid transporters (EAATs) are abundantly expressed by astrocytes, rapidly remove glutamate from the extracellular environment, and restrict temporal spatial extent of signaling. Studies probing EAAT function suggest that their capacity to is large does not saturate, even with substantial challenges. In contrast, we report neuronal activity reversibly modulates EAAT-dependent transport. To date, no physiological manipulation has shown changes in functional uptake a nonpathological state. Using iGluSnFr-based imaging electrophysiology adult mouse cortex, show slowed up threefold following bursts activity. The slowing depends on frequency duration presynaptic but independent amount released. modulation brief, returning normal within 50 ms after stimulation ceases. Interestingly, specific activated synapses, domain an individual astrocyte. Activity-induced uptake, increased persistence space, reflected decay times NR2A-mediated NMDA currents. These results astrocytic clearance temporally spatially manner ≥30 Hz these affect response released glutamate. This suggests previously unreported form neuron–astrocyte interaction. SIGNIFICANCE STATEMENT We first fast, astrocyte kinetics. cerebral cortex increases space its astrocytes. Because abundant expression, been thought have invariant While multiple studies experimental manipulations resulting altered our findings dynamic fast time-scale. shows rapid plasticity clearance, which locally synaptic signaling cortex. As fundamental essential mechanism for neurotransmission, this work implications extrasynaptic receptor activation, plasticity.

Language: Английский

Citations

123
Fast calcium sensor proteins for monitoring neural activity DOI Creative Commons
Aleksandra Badura, Xiaonan Richard Sun, Andrea Giovannucci

et al.

Neurophotonics, Journal Year: 2014, Volume and Issue: 1(2), P. 025008 - 025008

Published: Oct. 17, 2014

A major goal of the BRAIN Initiative is development technologies to monitor neuronal network activity during active information processing. Toward this goal, genetically encoded calcium indicator proteins have become widely used for reporting in preparations ranging from invertebrates awake mammals. However, slow response times, narrow sensitivity range Ca2+ and some cases, poor signal-to-noise ratio still limit their usefulness. Here, we review recent improvements field neural activity-sensitive probe design with a focus on GCaMP family proteins. In context, present our newly developed Fast-GCaMPs, which up 4-fold accelerated off-responses compared next-fastest GCaMP, GCaMP6f. Fast-GCaMPs were designed by destabilizing association hydrophobic pocket calcium-bound calmodulin RS20 binding domain, an intramolecular interaction that protects green fluorescent protein chromophore. Fast-GCaMP6f-RS06 Fast-GCaMP6f-RS09 rapid stopped-flow fluorimetry, neocortical brain slices, intact cerebellum vivo. Fast-GCaMP6f variants should be useful tracking action potentials closely spaced time, following fast-changing compartments, such as axons dendrites. Finally, discuss strategies may allow wider firing rates improve spike detection.

Language: Английский

Citations

111
Comprehensive Characterization of the Major Presynaptic Elements to the Drosophila OFF Motion Detector DOI Creative Commons
Étienne Serbe, Matthias Meier, Aljoscha Leonhardt

et al.

Neuron, Journal Year: 2016, Volume and Issue: 89(4), P. 829 - 841

Published: Feb. 1, 2016

Language: Английский

Citations

110
Orientation Selectivity Sharpens Motion Detection in Drosophila DOI Creative Commons
Yvette E. Fisher, Marion Silies, Thomas R. Clandinin

et al.

Neuron, Journal Year: 2015, Volume and Issue: 88(2), P. 390 - 402

Published: Oct. 1, 2015

Language: Английский

Citations

108
Inference of neuronal functional circuitry with spike-triggered non-negative matrix factorization DOI Creative Commons
Jian K. Liu,

Helene M. Schreyer,

Arno Onken

et al.

Nature Communications, Journal Year: 2017, Volume and Issue: 8(1)

Published: July 20, 2017

Neurons in sensory systems often pool inputs over arrays of presynaptic cells, giving rise to functional subunits inside a neuron's receptive field. The organization these provides signature the connectivity and determines how neuron integrates stimuli. Here we introduce method spike-triggered non-negative matrix factorization for detecting layout within only requires spiking responses under finely structured stimulation is therefore applicable large populations simultaneously recorded neurons. Applied recordings from ganglion cells salamander retina, retrieves fields bipolar as verified by simultaneous cell recordings. identified subunit layouts allow improved predictions natural stimuli reveal shared input into distinct types cells.How information knowledge about its connections. authors report new using identify onto retinal predict their

Language: Английский

Citations

107