Genome-wide association study, population structure, and genetic diversity of the tea plant in Guizhou Plateau

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Jan. 30, 2024

Abstract Background Guizhou Plateau, as one of the original centers tea plant, has a profound multi-ethnic cultural heritage and abundant germplasm resources. However, impact indigenous community factors on genetic diversity, population structure geographical distribution plant is still unclear. Results Using genotyping-by-sequencing (GBS) approach, we collected 415 accessions from study sites, estimated developed core collection, conducted genome-wide association (GWAS) based 99,363 high-quality single-nucleotide polymorphisms (SNPs). A total were clustered into six populations (GP01, GP02, GP03, GP04, GP05 GP06), results showed that GP04 had highest lowest diversity ( Pi = 0.214 0.145, respectively). Moreover, 136 (33%) selected to construct set can represent whole collection. By analyzing seven significant SNP markers associated with traits such germination period bud two leaves (OTL) three (OtL), four candidate genes possibly related OTL OtL identified. Conclusions This revealed communities accessions, indicating importance practices for protection utilization Four potential also identified, which will facilitate research, conservation, breeding.

Language: Английский

---

Temperature Dependence of Membrane Permeability Parameters for Five Cell Types Using Nonideal Thermodynamic Assumptions to Mathematically Model Cryopreservation Protocols

The Journal of Physical Chemistry B, Journal Year: 2024, Volume and Issue: 128(5), P. 1139 - 1160

Published: Jan. 31, 2024

Cryopreservation is the process of preserving biological matter at subzero temperatures for long-term storage. During cryopreservation, cells are susceptible to various injuries that can be mitigated by controlling cooling and warming profiles cryoprotective agent (CPA) addition removal procedures. Mathematical modeling changing cell volume different greatly reduce experiments needed optimize cryopreservation protocols. Such mathematical requires as inputs membrane permeabilities water CPA osmotically inactive fraction cell. Since intra- extracellular solutions generally thermodynamically nonideal, our group has been incorporating osmotic virial equation model solution thermodynamics underlie change equations, adding second third coefficients grouped intracellular solute parameters must measured. In previous work, we reported methods obtain room temperature fitting experimental kinetic data with equations incorporated nonideal assumptions. relevant excursions occur temperatures, dependence plays an important role. this present a new two-part method five cell-type-specific (water permeability, dimethyl sulfoxide fraction, solution) from measurements equilibrium function time 0 °C types, namely, human umbilical vein endothelial (HUVECs), H9c2 rat myoblasts, porcine corneal (PCECs), Jurkat T-lymphocyte line, cerebral microvascular (hCMECs/D3 line). The in work based on both data, enabling us solve some technical challenges expand previously measurement technique °C. Finally, use measured changes HUVEC protocol demonstrate impact thermodynamic assumptions predicting during freezing thawing.

Language: Английский

---

Conservation Mitonuclear Replacement: Facilitated mitochondrial adaptation for a changing world

Evolutionary Applications, Journal Year: 2024, Volume and Issue: 17(3)

Published: March 1, 2024

Abstract Most species will not be able to migrate fast enough cope with climate change, nor evolve quickly current levels of genetic variation. Exacerbating the problem are anthropogenic influences on adaptive potential, including prevention gene flow through habitat fragmentation and erosion diversity in small, bottlenecked populations. Facilitated adaptation, or assisted evolution, offers a way augment variation via artificial selection, induced hybridization, engineering. One key source variation, particularly for climatic core metabolic genes encoded by mitochondrial genome. These influence environmental tolerance heat, drought, hypoxia, but must interact intimately co‐evolve suite important nuclear genes. coadapted mitonuclear form some reproductive barriers between species. Mitochondrial genomes can do introgress an manner, they may co‐introgress maintaining compatibility. Managers should consider relevance variability conservation decision‐making, as tool facilitating adaptation. I propose novel technique dubbed Conservation Mitonuclear Replacement (CmNR), which entails replacing machinery threatened species—the genome loci—with those from closely related divergent population, better‐adapted changes carry lower load. The most feasible route CmNR is combine CRISPR‐based editing replacement technologies. This method preserves much organism's phenotype could allow populations persist wild when no other suitable options exist. mountaintops, where rising temperatures threaten alarming number almost certain extinction next century.

Language: Английский

---

The wildlife biobanking of germ cells for in situ and ex situ conservation in Japan

Theriogenology Wild, Journal Year: 2024, Volume and Issue: 4, P. 100086 - 100086

Published: Jan. 1, 2024

Japan arcs from north to south and is abundant in biodiversity with different climates each region. has many endemic species/populations, such as the Tsushima leopard cat (Prionailurus bengalensis euptilurus) Okinawa rail (Hypotaenidia okinawae), which threaten become endangered. Furthermore, one of countries highest number zoos aquariums world keeps wildlife species, are endangered, captive environments. Effective management genetic diversity acceleration reproduction essential for conservation endangered both inside outside their natural habitats. Germ cell preservation assisted reproductive technologies have great potential rescue resources contribute species. The long-term germ cells reviewed here context maintenance animals that died environments or habitats Japan. Here, we present a review our cross-disciplinary strategy conserve species using resource genomic endocrine research.

Language: Английский

---

FOGS: A SNPSTR Marker Database to Combat Wildlife Trafficking and a Cell Culture Bank for Ex‐Situ Conservation

Molecular Ecology Resources, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 10, 2025

ABSTRACT Illegal wildlife trade is a growing problem internationally. Poaching of animals not only leads to the extinction populations and species but also has serious consequences for ecosystems economies. This study introduces molecular marker system that authorities can use detect substantiate trafficking. SNPSTR markers combine short tandem repeats with single nucleotide polymorphisms within an amplicon increase discriminatory power. Within FOGS (Forensic Genetics Species Protection) project, we have established sets 74 vertebrate species. On average, each set consists 19 82 SNPs per set. More than 1300 over 300 STR were identified. Also, through its biobanking pipeline, project enabled cryopreservation somatic cells from 91 as well viable tissues later cell initiation further 109 species, providing future strategies ex situ conservation. In addition, many more fixed DNA samples endangered biobanked. Therefore, was interdisciplinary study, combining forensics conservation tools. The culture information are accessible database ( https://fogs‐portal.de/data ) open scientists, researchers, breeders worldwide protect illegal trade.

Language: Английский

---

When to cryopreserve and when to let it go? A systematic review of priorities in wild animal cryobanking

Theriogenology Wild, Journal Year: 2025, Volume and Issue: unknown, P. 100119 - 100119

Published: Jan. 1, 2025

Language: Английский

---

In vitro micropropagation protocols for two endangered Dianthus species - via in vitro culture for conservation and recultivation purposes

Plant Methods, Journal Year: 2025, Volume and Issue: 21(1)

Published: Feb. 5, 2025

Abstract Background D. giganteiformis subsp. pontederae and superbus are protected or critically endangered species in several European regions; therefore, developing an efficient vitro micropropagation protocol is essential for germplasm conservation recultivation purposes. Results After germination, one-nodal segments of both were transferred onto MS media supplemented with 3% sucrose different types cytokinins (at a concentration 4.5 µM) alongside 0.54 µM 1-naphthaleneacetic acid (NAA) the multiplication phase 3 weeks. The shoot clusters subsequently elongation medium (plant growth regulator-free medium) Individual shoots separated from cultured on NAA 2% weeks rooting. Taking into account effects after-effects cytokinins, we found that most suitable cytokinin was N-(2-isopentenyl)-adenine (2-iP), while it meta-topolin (mT). Conclusions In methods developed two Dianthus ( ) by determining optimal type to be used during phase. protocols designed produce large quantities propagation material recultivation, educational, research purposes within three months.

Language: Английский

---

Successful Cryoprotectant‐Free Vitrification of Honey Bee (Apis mellifera) Drone Sperm With Royal Jelly Supplemented Extender

Veterinary Medicine and Science, Journal Year: 2025, Volume and Issue: 11(2)

Published: Feb. 6, 2025

ABSTRACT In trying to protect honey bee species and maintain genetic diversity, employing effective approaches for drone sperm conservation is crucial. Based on literature, cryopreservation extenders methods have not been fully optimized. Our research aim was enhance preservation by incorporating royal jelly (RJ) in the extender of cryoprotectant‐free vitrification method. Drone collected from adult bees (36 days old) using manual inversion Different concentrations RJ (0%, 0.5%, 1%, 2.5% 5%) were added extender. Diluted cryopreserved a technique where 10 µL aliquots diluted directly dropped into liquid nitrogen then stored. Data analysed based completely randomized design with ten replications. Sperm quality parameters, including motility, viability DNA damage, evaluated vitro. Queens artificially inseminated measure ability motile reach spermathecae. The results showed that 1% supplementation significantly enhanced motility reduced fragmentation compared control higher concentrations. Specifically, group resulted highest viability, while both groups maintained lower rates. treated notably number their conclusion, media may improve parameters post‐warming. findings provide valuable insights optimizing preservation, contributing these vital pollinators.

Language: Английский

---

Comparison of vitrification techniques for the formation of the first skin biobank of lesser grison (Galictis cuja Molina, 1782): Insights into species genetic conservation

Gene Reports, Journal Year: 2025, Volume and Issue: unknown, P. 102166 - 102166

Published: Feb. 1, 2025

Language: Английский

---

The Evolution of Conservation Biobanking: A Literature Review and Analysis of Terminology, Taxa, Location, and Strategy of Wildlife Biobanks Over Time

Biopreservation and Biobanking, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

Nearly one-third of flora, fauna, and funga species on Earth are threatened with extinction. In response, the prevalence repositories-often called "biobanks" or "genome resource banks"-for storing biological materials from has become more widespread. This research examined trends for (1) terminology, (2) taxa representation, (3) global distribution, (4) operational approach biobanks versus genome banks relating to zoos wildlife. Our literature search results indicate that although banking began earlier in 1990s, biobanking seen a surge publications over 3.5× since 2020. Genome bank articles were highly focused mammals (68%), while multi-taxonomic overviews less-studied taxa. parameters found largest number wildlife Europe (18) lowest South America (2), though likely impacted by being completed English. Additionally, only 28% (7/25) biodiversity hotspots contain biobank based our methodology. While not all efforts published reported, these findings suggest "biobank" will be widely used term future, is needed non-mammalian taxa, there geographical gaps biobanks, conservation programs should focus biospecimens wide set individuals develop assisted reproductive technologies concomitantly goal maintaining healthy, sustainable populations long term.

Language: Английский

---