Review: Recent advances in bovine in vitro embryo production: reproductive biotechnology history and methods

animal, Journal Year: 2019, Volume and Issue: 14(5), P. 991 - 1004

Published: Nov. 25, 2019

In vitro production (IVP) of embryos and associated technologies in cattle have shown significant progress recent years, part driven by a better understanding the full potential these tools end users. The combination IVP with sexed semen (SS) genomic selection (GS) is being successfully widely used North America, South America Europe. main advantages offered include higher number pregnancies per unit time, wider range female donors from which to retrieve oocytes (including open cyclic females ones up 3 months pregnant), including high index calves, reduced sperm required produce increased chances obtaining desired sex offspring. However, there are still unresolved aspects that limit implementation technology, potentially fertility use SS, oocyte quality after maturation lower embryo cryotolerance, resulting pregnancy rates compared vivo–produced embryos. Nevertheless, promising research results been reported, work address current deficiencies. GS, SS has proven successful commercial field several countries assisting practitioners producers improve reproductive performance, efficiency genetic gain.

Language: Английский

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Tetraspanins, More than Markers of Extracellular Vesicles in Reproduction

International Journal of Molecular Sciences, Journal Year: 2020, Volume and Issue: 21(20), P. 7568 - 7568

Published: Oct. 14, 2020

The participation of extracellular vesicles in many cellular processes, including reproduction, is unquestionable. Although currently, the tetraspanin proteins found are mostly applied as markers, increasing evidence points to their role vesicle biogenesis, cargo selection, cell targeting, and uptake under both physiological pathological conditions. In this review, we bring other insight into involvement physiology mammalian reproduction. We provide knowledge regarding tetraspanins these processes somatic cells. Furthermore, discuss future direction towards an understanding functions tissues fluids reproductive system gamete maturation, fertilization, embryo development; mutual contact communication complexity.

Language: Английский

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Extracellular vesicle mediated embryo-endometrial cross talk during implantation and in pregnancy

Journal of Assisted Reproduction and Genetics, Journal Year: 2018, Volume and Issue: 36(2), P. 189 - 198

Published: Oct. 25, 2018

Language: Английский

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Deciphering the oviductal extracellular vesicles content across the estrous cycle: implications for the gametes-oviduct interactions and the environment of the potential embryo

BMC Genomics, Journal Year: 2018, Volume and Issue: 19(1)

Published: Aug. 22, 2018

The success of early reproductive events depends on an appropriate communication between gametes/embryos and the oviduct. Extracellular vesicles (EVs) contained in oviductal secretions have been suggested as new players mediating this crucial cross-talk by transferring their cargo (proteins, mRNA small ncRNA) from cell to cell. However, little is known about EVs (oEVS) composition implications success. aim study was determine oEVs content at protein, RNA level examine whether under hormonal influence estrous cycle.We identified presence oEVs, exosomes microvesicles, bovine fluid different stages cycle (postovulatory-stage, luteal phase, late phase pre-ovulatory stage) demonstrated that regulation. RNA-sequencing 903 differentially expressed transcripts (FDR < 0.001) across cycle. Moreover, RNA-Seq types ncRNAs (miRNAs, rRNA fragments, tRNA snRNA, snoRNA, other ncRNAs), which were partially also influence. Major differences found post-ovulatory rest analyzed for mRNAs. Interesting miRNAs showing differential abundance among stages, miR-34c miR-449a, associated with defective cilia oviduct infertility. Furthermore, functional annotation abundant mRNAs functions related exosome/vesicles, expression, embryo development many encoding ribosomal proteins. analysis protein revealed changes Mass spectrometry 336 clusters proteins 170 (p-value< 0.05, ratio 0.5 or > 2). Our data gamete-oviduct interactions well numerous proteins.Our provides first molecular signature cycle, revealing marked post- stages. findings contribute a better understanding potential role modulators gamete/embryo-maternal

Language: Английский

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An oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming

Nature Communications, Journal Year: 2018, Volume and Issue: 9(1)

Published: Nov. 16, 2018

Abstract Worldwide over 5 million children have been conceived using assisted reproductive technology, and research has concentrated on increasing the likelihood of ongoing pregnancy. However, studies animal models indicated undesirable effects in vitro embryo culture offspring development health. In vivo, oviduct hosts a period which early undergoes complete reprogramming its (epi)genome preparation for reacquisition (epi)genetic marks. We designed an oviduct-on-a-chip platform to better investigate mechanisms related degree they differ between vivo embryos. The device supports more physiological (in vivo-like) zygote genetic than conventional IVF. This approach will be instrumental identifying investigating factors critical fertilization pre-implantation development, could improve quality integrity IVF zygotes with likely relevance embryonic later fetal development.

Language: Английский

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Preparation and characterization of extracellular vesicles

American Journal of Reproductive Immunology, Journal Year: 2020, Volume and Issue: 85(2)

Published: Oct. 29, 2020

Abstract Extracellular vesicles (EVs) are heterogeneous membranous secreted by every cell type and offer significant potential in therapy diagnostics. Differential ultracentrifugation is the gold standard for EV isolation, although other techniques including, polyethylene glycol (PEG) precipitation, immunoprecipitation, size exclusion chromatography, immuno‐isolation approaches common. Purified EVs can be characterized based on their physical characteristics, biochemical composition, or of origin. For concentration measurement, nanoparticle tracking analysis (NTA), dynamic light scattering (DLS), electron microscopy commonly employed methods. Biochemical analyses typically performed using flow cytometry, immunoblotting, proteomic investigation. Based tissue origin, have specific markers that used to isolate purify cell‐associated an affinity selection approach. Despite existence several methods isolation characterization, major limitations associated with each method hinder progress field. Evolving concepts biology possess great promise better characterization leading a insight biological function immense clinical implications. In this review, we discuss recent advancements approaches.

Language: Английский

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Oviductal extracellular vesicles interact with the spermatozoon’s head and mid-piece and improves its motility and fertilizing ability in the domestic cat

Scientific Reports, Journal Year: 2019, Volume and Issue: 9(1)

Published: July 1, 2019

Fertilization and early embryo development are regulated by a unique maternal-gamete/embryo cross-talk within the oviduct. Recent studies have shown that extracellular vesicles (EVs) oviduct play important roles in mediating this developmental process. Here, we examined influence of oviductal EVs on sperm function domestic cat. We demonstrated (1) enriched proteins related to energy metabolism, membrane modification, reproductive function; (2) bound fused with membranes acrosome mid piece; (3) incubating improved motility, fertilizing capacity cat spermatozoa prevented acrosomal exocytosis vitro. These findings indicated mediate fertilization provides new insights improve cryopreservation vitro wild felids human.

Language: Английский

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Long term effects of ART: What do animals tell us?

Molecular Reproduction and Development, Journal Year: 2018, Volume and Issue: 85(4), P. 348 - 368

Published: Feb. 21, 2018

Early stages of mammalian embryonic development are now known to be very sensitive their microenvironment, with long term effects on fetal, postnatal, and adult health, thus extending these early the concept Developmental Origin Health Disease (DoHaD). In this scientific context, 3% births in developed countries, safety Assisted Reproductive Techniques procedures becomes a matter concern. Besides, embryo technologies domestic mammals, using huge number embryos, do not seem evidence heavy impacts phenotypes. This paper first discusses what can or cannot concluded from farm animal data, then develops ART (ovarian stimulation, vitro fertilization culture) evidenced model species (mainly mouse model). Recent literature demonstrates both individual cumulative each procedure fetal postnatal second part, because they sources for further perturbations, immediate phenotypes at cellular molecular levels described animals species. Mechanistic hypotheses supporting induced phenotypic alterations subsequently considered. Finally, taking into account interspecies differences mechanisms likely involved, relevance results obtained models human discussed.

Language: Английский

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Composing the Early Embryonic Microenvironment: Physiology and Regulation of Oviductal Secretions

International Journal of Molecular Sciences, Journal Year: 2019, Volume and Issue: 21(1), P. 223 - 223

Published: Dec. 28, 2019

The oviductal fluid is the first environment experienced by mammalian embryos at very beginning of life. However, it has long been believed that was not essential for proper embryonic development. Successful establishment in vitro embryo production techniques (which completely bypass oviduct) have reinforced this idea. Yet, became evident produced differ markedly from their vivo counterparts, and these differences are associated with lower pregnancy outcomes more health issues after birth. Nowadays, researchers consider oviduct as most suitable microenvironment early development a substantial effort made to understand its dynamic, species-specific functions. In review, we touch on origin molecular components mammals, where recent progress thanks wider use mass spectrometry techniques. Some factors processes known regulate secretions, including itself, well ovulation, insemination, endogenous exogenous hormones, metabolic heat stress, summarized. Special emphasis laid farm animals because, owing availability sample material economic importance fertility livestock husbandry, large part work topic carried out domestic used dairy and/or meat production.

Language: Английский

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Roles of steroid hormones in oviductal function

Reproduction, Journal Year: 2019, Volume and Issue: 159(3), P. R125 - R137

Published: Oct. 18, 2019

The oviduct (known as the fallopian tube in humans) is site for fertilization and pre-implantation embryo development. Female steroid hormones, estrogen progesterone, are known to modulate morphology function of cells oviduct. In this review, we focus on actions progesterone secretory, ciliated, muscle cell functions morphologies during fertilization, development, transport humans, laboratory rodents farm animals. We review some aspects oviductal anatomy histology discuss current assisted reproductive technologies (ARTs) that bypass their effects quality. Lastly, causes alterations could result defects.

Language: Английский

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