The effect of the use of vacuum therapy and instillation of L-arginine in the treatment of purulent wounds on the activity of nitric oxide cycle enzymes

EMERGENCY MEDICINE, Journal Year: 2025, Volume and Issue: 21(1), P. 71 - 76

Published: March 15, 2025

Background. The problem of treating purulent wounds remains one the most urgent throughout history surgery. Nowadays, despite many years experience and constant scientific research, diagnosis treatment does not lose its relevance. purpose is to evaluate effect vacuum therapy instillation L-arginine in a wound on production nitric oxide by different isoforms NO-synthase activity arginase pathway metabolism. ­Materials methods. experiment was conducted 20 patients who were randomly divided into 2 groups: (GR-1, n = 10) with instillations solution (GR-2, 10). In blood plasma, following parameters investigated: total NO-synthase, inducible constitutive arginases nitrite concentration. Results. When comparing results obtained after from GR-1 GR-2, statistically significant difference found concentration nitrites arginases. Thus, venous GR-2 45.59 % lower than GR-1. plasma taken bed closest 25.23 higher compared indicators Conclusions. Vacuum instillation, addition effects itself, limits accumulation enhances metabolism pathway.

Language: Английский

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INFLUENCE OF CHROMIUM PICOLINATE ON THE FUNCTIONING OF THE NITRIC OXIDE CYCLE IN THE BICEPS FEMORIS MUSCLE OF RATS UNDER CONDITIONS OF METABOLIC SYNDROME

The Medical and Ecological Problems, Journal Year: 2025, Volume and Issue: 29(1), P. 13 - 18

Published: April 30, 2025

Obesity can trigger the onset of a key pathogenetic feature metabolic syndrome, known as inflammation (metainflammation). During metainflammation residual macrophages undergo changes in their polarization state, leading to either increase arginase or inducible NO-synthase activities, enzymes nitric oxide cycle. Thus, syndrome development may influence state cycle various organs and tissues. The aim this study is evaluate effect chromium picolinate on activities NO-cycle (NO-synthases, nitrate nitrite reductases, arginases) content metabolites rat biceps femoris muscle during modelling. Materials methods. was conducted 24 mature male Wistar rats, weighing between 200 260 g. animals were divided into 4 groups 6 each: I – Control group; II Metabolic group. reproduced by using 20% fructose solution only source water for 60 days. III Chromium administration administered daily orally dose 80 µg/kg IV Administration object 10% homogenate rats. We studied following biochemical parameters: total, constitutive activity, reductase concentration nitrites, peroxynitrites, nitrosothiols. Results. with experimentally induced resulted 32.30% decrease total activity compared untreated Activity decreased 34.26%. NO-synthases increased 45.16%. Arginase reduced 41.15%. Nitrate 25.57% 31.45%, respectively. Nitrite 117.97%. Peroxynitrite 21.79%. Nitrosothiols 34.15%. Conclusions. modeling attenuates excessive production influencing both L-arginine-dependent L-arginine-independent pathways its production.

Language: Английский

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CHROMIUM PICOLINATE PREVENTS OXIDATIVE DAMAGE TO CARDIOMYOCYTES UNDER CONDITIONS OF METABOLIC SYNDROME MODELING

Актуальні проблеми сучасної медицини Вісник Української медичної стоматологічної академії, Journal Year: 2025, Volume and Issue: 25(2), P. 136 - 140

Published: May 29, 2025

Introduction. Metabolic syndrome is a systemic pathology commonly associated with eating disorders that lead to excess calorie consumption. In addition the development of obesity, metabolic often myocardial damage. One universal mechanisms cell damage oxidative stress. The search for proper medication will prevent myocardium and influence pathogenetic links in relevant area research. Chromium picolinate promising agent preventing syndrome, as it may enhance lipolysis adipocytes. Objectives. aim this work determine effect chromium on production superoxide anion radical, activity antioxidant enzymes, content malondialdehyde heart rats under conditions experimental syndrome. Materials methods. study was performed 24 Wistar weighing 200–260 g. animals were assigned four groups (n = 6 per group): control group; model group, which syndrome(MS) induced by administering 20% fructose solution sole source drinking water standard vivarium diet 60 days; received orally at dose 80 μg/kg daily combined receiving both MS-inducing regimen. Cardiac stress parameters assessed 10% homogenates. following indicators measured: basal dismutase (SOD) catalase (CAT), (MDA) content. Results. Administration modeled reduces radical 52.33% compared group. Under these conditions, NADPH-induced NADHinduced are reduced 20.13% 20.69%, respectively, increases 78.88% 137.93%, decreases 25.59% OMP does not change statistically significantly. use effectively reactive oxygen species, enhances defense, intensity lipid peroxidation This part initiative research project No. 0124U000092 “Highand low-intensity phenotypes inflammatory response: molecular new medical technologies their prevention correction”.

Language: Английский

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IMPACT OF QUERCETIN ON THE ORGANIC MATRIX AND BIOMECHANICAL PROPERTIES OF RAT MANDIBLE FOLLOWING ITS INCOMPLETE FRACTURE DURING CHRONIC ALCOHOL INTOXICATION

Fìzìologìčnij žurnal, Journal Year: 2024, Volume and Issue: 70(3), P. 51 - 58

Published: April 22, 2024

This article investigates the impact of water-soluble form quercetin (corvitin) on depolymerization organic matrix biopolymers and biomechanical properties rat mandible following its incomplete fracture during chronic alcohol intoxication. The study was conducted 14th day after inducing intoxication by daily intragastric administration a 40% ethanol solution in dose 12 ml/kg body weight; that modelled drilling bone to depth 1 mm using carbide drill with diameter 1.5 at speed up 1000 rpm. for 14 days posttraumatic period 10 mg/kg (in recalculation quercetin), an mandibular intoxication, significantly reduced activity acid phosphatase tartrate-resistant isoform 35.3% 23.1%, respectively, as well led decrease concentration free hydroxyproline, hexuronic N-acetylneuraminic acids 17.0%, 30.6%, 43.3%, compared findings revealed animals, which did not receive drug. Young’s modulus exceeded values this group 72.3%, elastic limit 53.2% tensile strength 59.6%, relative elongation failure 30.0%. We concluded improves reparative processes injured area; it also restricts resorption connective (bone) tissue such collagen, proteoglycans, sialoglycoproteins. Under these conditions, area, particularly elasticity strength, are enhanced.

Language: Английский

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Nitric oxide cycle activity in rat biceps femoris muscle under conditions of bacterial lipopolysaccharide influence, experimental metabolic syndrome and their combination

The Ukrainian Biochemical Journal, Journal Year: 2023, Volume and Issue: 95(4), P. 24 - 34

Published: Sept. 11, 2023

There is evidence that long-term organism stimulation with bacterial lipopolysaccharides (LPS), which promotes the secretion of pro-inflammatory cytokines and nitric oxide, may play an important role in metabolic syndrome (MetS) development. Changes NO production under conditions MetS have different directions depend on a specific organ. The purpose this work was to study oxide its metabolites biceps femoris muscle rats lipopolysaccharide organism, their combination. conducted for 60 days 24 male Wistar divided into control, MetS, LPS LPS+MetS groups. reproduced by adding 20% fructose solution food, carried out intraperitoneal injection S. typhi LPS. It demonstrated rat experimental increased L-arginine-dependent pathway, but limited syndrome-induced increase L-arginine-independent reduced concentration S-nitrosothiols, while increasing peroxynitrites nitrites rats. Keywords: lipopolysaccharide, femoris, syndrome, reactive nitrogen species

Language: Английский

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Influence of doxorubicin on the extracellular matrix of the liver of rats under conditions of chronic alcoholic hepatitis

Regulatory Mechanisms in Biosystems, Journal Year: 2023, Volume and Issue: 14(2), P. 278 - 283

Published: April 14, 2023

The liver has a high regenerative potential that is dependent on many factors, in particular adenosine monophosphate kinase signaling, however, long-term alteration, such as daily alcohol consumption, turns regeneration into chronic disease fibrosis, the end stage of which cirrhosis. Hepatic extracellular matrix proteins are important triggers enhanced stellate cell function during progression fibrosis. experiments were performed Wistar rats divided four groups: control group; doxorubicin injection group (1.25 mg/kg); hepatitis alcoholic group, where we studied total concentration glycosaminoglycans, heparin-heparan, keratan-dermatan and chondroitin fractions free oxyproline sialic acids tissue homogenate. Cells with Mallory-Denk bodies present from consisted tangled balls intermediate filaments showed eosinophilia cytoplasm degenerative hepatocytes. There also necrotic changes cells. Sinusoidal capillaries locally dilated. In central sections lobules injected against background hepatitis, veins expanded, their endothelium was thinned. there no blood cells lumens. nuclei vast majority hepatocytes state karyopyknosis, karyorrhexis, karyolysis. Administration to animals leads an increase sulfates, decrease heparin-heparan fraction glycosaminoglycans compared group. concentrations fraction, significantly increased, content decreased hepatitis. by 1.25 times, – 1.36 ethanol or combination causes morphological characteristic lobules. administration prevents alcohol-induced collagenolysis breakdown glycoproteins, but increases proteoglycans due fractions.

Language: Английский

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Role of sulfide anion in the development of chronic alcoholic hepatitis under the conditions of modulation of adenosine monophosphate kinase – a correlational study

European Journal of Clinical and Experimental Medicine, Journal Year: 2023, Volume and Issue: 21(3), P. 567 - 575

Published: Sept. 1, 2023

Introduction and aim. Hydrogen sulfide (H 2 S) has attracted the attention of researchers as a novel signaling molecule that affects vascular metabolism, immune function, stress inflammation. It plays an important role in pathophysiological disorders under conditions development obesity, diabetes, non-alcoholic fatty liver disease cardiovascular diseases. The purpose this work is to establish correlation ratios H S concentration with markers oxidative-nitrosative extracellular matrix metabolism during chronic alcoholic hepatitis modeling AMPK modulation by phenformin doxorubicin. Material methods. experiments were performed on 36 white, sexually mature male Wistar rats, weighing 180-220 g. Alcoholic was modelled alcohol administration, background animals received orally at dose 10 mg/kg or doxorubicin 1.25 intraperitoneally. Statistical processing results biochemical studies carried out using non-parametric method Spearman determine correlations. Results. inversely proportionally strongly correlates nitrites, oxyproline arginase activity. Phenformin administration leads formation production superoxide anion radical, malondialdehyde, activities constitutive NO-synthases, nitrite reductases, nitrate arginase. Doxorubicin directly proportional activity reductases. Conclusion. Administration expands correlations between indicators stress.

Language: Английский

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IMPACT OF NF-κB AND NRF2 TRANSCRIPTION FACTOR MODULATORS ON METABOLIC CHARACTERISTICS IN MANDIBLE BONES OF RATS DURING RECOVERY FROM INCOMPLETE FRACTURE UNDER CHRONIC ALCOHOL INTOXICATION

Актуальні проблеми сучасної медицини Вісник Української медичної стоматологічної академії, Journal Year: 2024, Volume and Issue: 24(1), P. 114 - 118

Published: March 20, 2024

This study aims at investigating the influence of specific modulators NF-κB and Nrf2 transcription factors on oxidative-nitrosative stress indicators bone biopolymer depolymerization in mandibular following incomplete fracture under chronic alcohol intoxication (CAI). Twenty-eight white male Wistar rats were divided into five groups: Group 1 comprised "falsely injured" rats, while 2 underwent (IMF) CAI exposure. Rats groups 3 4 received intraperitoneal injections ammonium pyrrolidinium dithiocarbamate, an inhibitor activation, a dose 76 mg/kg, dimethyl fumarate, inducer, 15 mg/kg three times week for 14 days modeling IMF The activity total NO synthase, including its constitutive inducible isoforms, along with ornithine decarboxylase, concentrations peroxynitrites alkaline alkaline-earth metals, free hydroxyproline, N-acetylneuraminic, hexuronic acids assessed homogenate standard mandible area using spectrophotometric method. findings have demonstrated that administration pyrrolidine dithiocarbamate fumarate notably decreased synthase (primarily isoform) concentration peroxynitrite homogenate, increasing key enzyme polyamine biosynthesis. Furthermore, experimental conditions, use limited biopolymers (collagen, glycoproteins, proteoglycans), thereby facilitating effective reparative osteogenesis.

Language: Английский

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ROLE OF NF-κB ACTIVATION IN CHANGES OF NITRIC OXIDE PRODUCTION IN SKELETAL MUSCLES DURING METABOLIC SYNDROME

Актуальні проблеми сучасної медицини Вісник Української медичної стоматологічної академії, Journal Year: 2024, Volume and Issue: 24(3), P. 94 - 98

Published: Nov. 11, 2024

Nitric oxide, as a signaling molecule, plays an ambiguous role in many pathological processes. On the one hand, nitric oxide is necessary for maintaining tone of vascular wall arteries and prevents development ischemia various organs skeletal muscles particular. other excessive production tissue can contribute to formation toxic metabolites, such peroxynitrite that leads nitrosative damage tissues. Metabolic syndrome also diseases accompanied by disturbances system. Currently, sources production, predominant ways its metabolism, influence transcription factor NF-κB on these processes under conditions metabolic are insufficiently studied. The purpose this study effect inhibitor activation concentration metabolites biceps femoris muscle rats modeling syndrome. experimental was carried out male Wistar weighing 200-260 g. animals were randomly divided into 4 groups 6 each. Animals group 1 (control group) received standard vivarium diet. In 2 (metabolic syndrome), addition diet, 20% fructose solution their sole source drinking water 60 days. 3, given diet injected intraperitoneally with ammonium pyrrolidine dithiocarbamate at dose 76 mg/kg, three times week Group same along injections administered 3. 10% homogenate muscle, following determined: total NO-synthase activity, activity constitutive inducible isoforms NO-synthase, arginase aitrate- nitrite reductase concentrations nitrite, peroxynitrite, nitrosothiols, sulfides. administration pyrrolidinedithiocarbamate during simulation resulted reduction 33.85% 34.66%, respectively, compared group; decreased 37.56%, while nitrate activities reduced 19.29% 47.71%, respectively; increased 21.77%, 32.05%, nitrosothiol 29.27%, sulfide 17.39% Activation through both L-arginine-dependent L-arginine-independent pathways enhances results elevated formation.

Language: Английский

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Influence of organism stimulation with bacterial lipopolysaccharide on nitric oxide production and metabolism in rat heart on the background of metabolic syndrome

Modern medical technology, Journal Year: 2023, Volume and Issue: 4, P. 45 - 50

Published: Dec. 7, 2023

Aim. The aim of the study was to establish changes in nitric oxide production and metabolism rat heart during combined influence organism stimulation with bacterial lipopolysaccharide (LPS) modeling metabolic syndrome (MetS). Materials methods. conducted on 24 mature male Wistar rats weighing 200–260 g. Experiment lasted 60 days. animals were divided into 4 groups 6 each: control group, MetS LPS + group. reproduced by using a 20 % fructose solution as only source drinking water. Salmonella typhi administered at dose 0.4 μg/kg intraperitoneally. Animals from group received water LPS. In 10 tissue homogenate we studied: total activity NO-synthases (NOS), constitutive (cNOS) inducible (iNOS) isoforms, nitrate (NaR) nitrite (NiR) reductases, concentration peroxynitrites (ONOO-), nitrites, nitrosothiols hydrogen sulfide. Results. Combination led increase NOS 32.72 compared Activity cNOS did not change iNOS increased 33.76 %. Arginase decreased 23.53 NaR NiR 86.67 149.29 %, respectively. decrease 38.73 54.79 Under these conditions ONOOelevated 398.0 Concentration H S 27.56 %. Conclusions. leads prevalence peroxynitrite formation NO-synthase-dependent nitrate-nitrite-NO pathways heart.

Language: Английский

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