Nanoemulsion is an effective antimicrobial for methicillin-resistant Staphylococcus aureus in infected swine skin burn wounds DOI Creative Commons

Zhengyi Cao,

Jesse Chen,

Jayme Cannon

et al.

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: 12(11)

Published: Oct. 14, 2024

ABSTRACT Burns are one of the most common injuries in both civilian and combat settings difficult to treat. This is particularly true when wounds infected with antibiotic-resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA). A new generation safe, broadly effective, easily applied anti-infection agents needed successfully prevent treat infections. Nanoemulsions (NEs) nanometer-sized particles a positively charged surfactant at their oil-water interface. In current study, we further investigated antimicrobial NEs treatment address burn by MRSA. Specifically, using porcine skin model, partial thickness thermal MRSA then treated it nanoemulsion formulation (NB-201) or placebo controls. Bacterial viability after was determined, inflammation indexes were scored histopathology. Topical NB-201 resulted reduced colony-forming units (CFUs) compared treatment. addition, effective significantly alleviating promoting wound healing. These results indicate that promising agent IMPORTANCE The findings this study focused on therapeutic applications nanotechnology. demonstrated could effectively kill (MRSA) infection wounds. Infection threat public health usually due limited therapies available. Therefore, finding has great potential make novel against

Language: Английский

Antibiotic Resistance of Staphylococcus aureus Strains—Searching for New Antimicrobial Agents—Review DOI Creative Commons
Michał Michalik,

Adrianna Podbielska‐Kubera,

Agnieszka Dmowska-Koroblewska

et al.

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(1), P. 81 - 81

Published: Jan. 11, 2025

Inappropriate and excessive use of antibiotics is responsible for the rapid development antimicrobial resistance, which associated with increased patient morbidity mortality. There an urgent need to explore new or alternative agents. S. aureus a commensal microorganism but also numerous infections. In addition innate resistance β-lactam antibiotics, strains resistant methicillin (MRSA) often show other classes (multidrug resistance). The advancement phage therapy against MRSA infections offers promising in context increasing antibiotic resistance. Therapeutic phages are easier obtain cheaper produce than antibiotics. However, there still lack standards ensure safe phages, including purification, dosage, means administration, quantity used. Some bacteria have developed defense mechanisms phages. cocktails combination preferred. For personalized therapy, it essential set up large collections enable selection. future, fight using should be based on multidisciplinary approach, molecular biology medicine. Other therapies include endolysin peptides (including defensins cathelicidins). Researchers’ activities focus potential plant extracts, honey, propolis, alkaloids, oils. To date, no vaccine has been approved strains.

Language: Английский

Citations

0
Draft genomes of six methicillin-resistant Staphylococcus aureus in a Philippine tertiary hospital with insights on putative antimicrobial and phage resistance mechanisms DOI Open Access
Mark B. Carascal,

JM Gonzales,

Ma. Mona Joy T. Tañedo

et al.

Microbiology Resource Announcements, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

The genomes of six methicillin-resistant Staphylococcus aureus (MRSA) revealed CC5(ST5)-SCCmec-IVc-spa-t105-PVL+ (n = 3), CC5(ST6)-SCCmec-IVc-spa-t10002 1), CC5(ST5)-SCCmec-IVa-spa-t002 and CC8(ST8)-SCCmec-IVa-spa-t008-PVL+ 1) genotypes. All possessed multiple antibiotic resistance genes (putative mecA blaZ, dfrG for cotrimoxazole-resistant strains), intact staphylococcal prophages, putative antiphage systems. results could aid in MRSA infection management control.

Language: Английский

Citations

0
Management of methicillin-resistant staphylococcus aureus bloodstream infections: a comprehensive narrative review of available evidence focusing on current controversies and the challenges ahead DOI
Alberto Enrico Maraolo, Milo Gatti, Luigi Principe

et al.

Expert Review of Anti-infective Therapy, Journal Year: 2025, Volume and Issue: unknown

Published: March 31, 2025

Bloodstream infections (BSIs) caused by Staphylococcus aureus are common worldwide, representing one of the most relevant issues in clinical infectious diseases practice. In particular, BSIs methicillin-resistant S. (MRSA-BSI) still today a challenge since mortality burden remains elevated although decades research. The following topics regarding MRSA-BSI were reviewed and discussed resorting to best available evidence retrieved from PubMed/MEDLINE up October 2024: i) epidemiology; ii) microbiology; iii) classification, with focus on complicated not forms; iv) structured approach patient; v) pharmacokinetics pharmacodynamics main antimicrobial options; vi) controversies therapeutic approach. Despite ongoing efforts better stratify manage MRSA-BSI, there is no universally accepted classification system accurately distinguishing between uncomplicated/low risk complicated/high forms. Biomarkers such as interleukin(IL)-10 hold promise order enable more precise stratification, premise for an appropriate treatment plan. There theoretical rationale implementing combination therapy including beta-lactam agent upfront, especially patients considered at higher unfavorable outcomes, but further data necessary, same applies newer adjuvants. Novel microbiological techniques may help guiding duration.

Language: Английский

Citations

0
Bacteriophage Therapy as a Promising Alternative for Antibiotic-Resistant Enterococcus faecium: Advances and Challenges DOI Creative Commons
Laura Ribes-Martínez, María-Carmen Muñoz-Egea, José Yuste

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 13(12), P. 1120 - 1120

Published: Nov. 23, 2024

Enterococcus faecium is a Gram-positive bacterium increasingly identified as critical nosocomial pathogen that poses significant treatment challenges due to its resistance multiple antibiotics, particularly vancomycin-resistant E. (VRE) strains. The urgent need for alternative therapeutic strategies has renewed interest in bacteriophage (phage) therapy, given phages specificity and bactericidal potential. This review explores the advancements phage therapy against antibiotic-resistant faecium, including morphological diversity, genomic characteristics, infection mechanisms. efficacy of vitro, ex vivo, vivo models compassionate use clinical settings are evaluated, highlighting promising outcomes phage–antibiotic synergies biofilm disruption. Key future research directions discussed, with focus on improving overcoming bacterial resistance. emphasizes potential viable solution managing multidrug-resistant infections underscores importance investigations enhance applications.

Language: Английский

Citations

2
Nanoemulsion is an effective antimicrobial for methicillin-resistant Staphylococcus aureus in infected swine skin burn wounds DOI Creative Commons

Zhengyi Cao,

Jesse Chen,

Jayme Cannon

et al.

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: 12(11)

Published: Oct. 14, 2024

ABSTRACT Burns are one of the most common injuries in both civilian and combat settings difficult to treat. This is particularly true when wounds infected with antibiotic-resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA). A new generation safe, broadly effective, easily applied anti-infection agents needed successfully prevent treat infections. Nanoemulsions (NEs) nanometer-sized particles a positively charged surfactant at their oil-water interface. In current study, we further investigated antimicrobial NEs treatment address burn by MRSA. Specifically, using porcine skin model, partial thickness thermal MRSA then treated it nanoemulsion formulation (NB-201) or placebo controls. Bacterial viability after was determined, inflammation indexes were scored histopathology. Topical NB-201 resulted reduced colony-forming units (CFUs) compared treatment. addition, effective significantly alleviating promoting wound healing. These results indicate that promising agent IMPORTANCE The findings this study focused on therapeutic applications nanotechnology. demonstrated could effectively kill (MRSA) infection wounds. Infection threat public health usually due limited therapies available. Therefore, finding has great potential make novel against

Language: Английский

Citations

0