Biomolecules,
Journal Year:
2025,
Volume and Issue:
15(2), P. 227 - 227
Published: Feb. 4, 2025
Hydrocarbon
fuel
biofouling
and
biocorrosion
require
expensive
cleanup
of
aviation
infrastructures
unless
appropriate
sustainment
measures
are
applied.
The
identification
novel
biological
control
agents
offers
promising
alternatives
to
the
current
chemical
biocides
used
in
sustainment.
In
this
study,
496
microbial
isolates
from
our
in-house
repository
were
screened
identify
new
endogenously
produced
antimicrobial
compounds.
Using
agar
plug
screening,
liquid
culture
growth
testing,
Jet
A
assays,
two
fuel-isolate
strains
Pseudomonas
protegens
#133,
Bacillus
subtilis
#232
demonstrated
biocontrol
activity
against
bacteria,
yeast,
filamentous
fungi.
Liquid
chromatography-quadrupole
time
flight
tandem
mass
spectrometry
(LC-QTOF-MS/MS)
filtrate
identified
several
common
lipopeptide
antimicrobials
including
gageostatin
C,
gageopeptin
B,
miscellaneous
macrolactins.
contrast,
LC-QTOF-MS/MS
siderophore
pyochelin
as
one
predominant
compounds
#133
with
previously
effect.
consortium
testing
flow-cytometry
live/dead
cell
mechanism
determination
action
Gram-positive
bacteria.
study
concludes
that
secreted
by
have
bactericidal
effects
Gordonia
sp.
cause
death
through
bacterial
lysis
membrane
damage
potential
applications
biocidal
treatment
hydrocarbon-based
fuels.
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(2), P. 860 - 860
Published: Jan. 20, 2025
Soil
salinization
poses
a
significant
challenge
to
global
agriculture,
particularly
in
arid
and
semi-arid
regions
like
Xinjiang.
Kalidium
schrenkianum,
halophytic
plant
adapted
saline-alkaline
conditions,
harbors
endophytic
microorganisms
with
potential
growth-promoting
properties.
In
this
study,
177
bacterial
strains
were
isolated
from
K.
11
key
identified
through
functional
screening
based
on
salt
tolerance,
nutrient
solubilization,
traits.
Synthetic
microbial
communities
(SMCs)
then
constructed
using
these
optimized
enhance
wheat
growth
under
stress.
The
SMCs
significantly
improved
seed
germination,
root
length,
seedling
vigor
both
spring
winter
hydroponic
pot
experiments.
Furthermore,
the
enhanced
activities
of
antioxidant
enzymes,
including
superoxide
dismutase
(SOD),
peroxidase
(POD),
catalase
(CAT),
levels
malondialdehyde
(MDA)
proline
(PRO).
They
also
reduced
oxidative
stress
chlorophyll
content
seedlings.
These
results
demonstrate
consortia
derived
extreme
environments
as
eco-friendly
biofertilizers
for
improving
crop
performance
saline
soils,
offering
sustainable
alternative
chemical
fertilizers
contributing
agricultural
resilience
productivity.
AMB Express,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: Jan. 27, 2025
Abstract
The
increasing
demand
for
natural
alternatives
to
synthetic
fungicides
has
prompted
research
into
products
like
essential
oils
postharvest
disease
management.
This
study
investigated
the
antifungal,
antioxidant,
cytotoxic,
and
genotoxic
potential
of
oil
mixtures
derived
from
oregano,
rosemary,
mint
against
Penicillium
digitatum
,
predominant
fungal
pathogen
causing
green
mold
in
orange
fruits.
P.
NPAGRASU
2024
was
isolated
identified
as
most
abundant
species
(60.2%)
infected
oranges.
It
deposited
GenBank
with
gene
accession
number
PP930644
MIRCEN
culture
collection
EMCC
358874.
antimicrobial
activity
individual
evaluated,
oregano
exhibiting
highest
antifungal
(inhibition
zone
diameter
4.2
cm)
.
L-optimal
mixture
design
response
surface
methodology
(RSM)
optimization
revealed
a
highly
effective
(Run
8)
comprising
46.26%
53.74%
99.65%
actual
growth
reduction.
demonstrated
potent
antioxidant
activity,
reaching
approximately
75%
DPPH
radical
scavenging
at
3.125
mg/mL.
Cytotoxicity
assessment
using
MTT
assay
showed
morphological
changes
reduced
cell
viability
liver
cells
treated
PEOs
300
µg/mL.
However,
optimized
did
not
induce
significant
chromosomal
aberrations
compared
control,
suggesting
minimal
effects.
In
vivo,
evaluation
on
oranges
60%
inhibition
by
1%
(v/v)
7
days.
Histological
analysis
indicated
low
toxicity
tested
concentration
(1%
mixture).
GC-MS
major
compounds
cavarcol,
caryophyllene,
eucalyptol,
phenols
levomenthol
oil,
contributing
its
bioactivities.
demonstrates
eco-friendly
control.
Pakistan Journal of Biological Sciences,
Journal Year:
2025,
Volume and Issue:
28(2), P. 95 - 101
Published: Jan. 15, 2025
<b>Background
and
Objective:</b>
Peatlands
are
unique
ecosystems
rich
in
microbial
diversity,
including
bacteria
with
potential
antibiotic
activity.
This
study
focuses
on
the
isolation
characterization
of
from
Indonesian
peat
soil,
particularly
their
to
produce
antibiotics
against
multidrug-resistant
(MDR)
pathogens,
Methicillin-Resistant
<i>Staphylococcus
aureus</i>
(MRSA).
<b>Materials
Methods:</b>
Bacterial
isolates
were
rejuvenated
nutrient
agar
subjected
antimicrobial
activity
testing
using
Bauer
&
Kirby
diffusion
method
MRSA.
The
bacterial
strain
exhibiting
strongest
was
further
analyzed
16S
rRNA
sequencing
for
genetic
identification.
Phylogenetic
analysis
performed
NCBI
BLAST,
followed
by
a
statistical
comparison
inhibition
zones
assess
efficacy.
<b>Results:</b>
Antimicrobial
revealed
that
isolate
10
PS
exhibited
larger
zone
MRSA
than
positive
control,
Ampicillin,
indicating
its
strong
potential.
confirmed
belonged
<i>Lysinibacillus</i>
genus,
though
significant
branch
divergence
suggested
it
may
represent
novel
species.
isolate's
lack
endospore
production,
typically
characteristic
alongside
peatland
ecosystem,
suggests
adaptations
environmental
pressures.
<b>Conclusion:</b>
These
findings
highlight
soil
as
valuable
source
antibiotics,
MDR
pathogens
like
proposed
new
species,
(cataloged
SUB14736623),
expands
taxonomic
knowledge
holds
promise
developing
natural
treatments.
