Antimicrobial potential of strontium against bacteria associated with peri-implantitis DOI Open Access
Hatem Alshammari

Published: Jan. 1, 2023

Dental implants are nowadays a standard treatment to replace missing teeth and restore function aesthetics. However, biological complications associated with implants, e.g., peri-implant infections, common jeopardize the success of treatment. The main aim this thesis was explore strontium (Sr) as possible prevention strategy against since Sr has been shown have antibacterial action also promote titanium (Ti) implant osseointegration. In thesis, systematic appraisal literature about antimicrobial potential Sr-functionalized Ti surfaces for oral applications performed, followed by series in vitro studies assessing micro-organisms peri-implantitis. Study I, resulted an initial list 1081 potentially relevant publications, where from nine publications met inclusion criteria. Most included showed that exerted limited immediate (i.e., 24 h) effect, likely due low ion release; effect biofilm inhibition Streptococcus aureus observed at both early late timepoints, adequate release. II assessed, vitro, five different concentrations soluble Sr(OH)2 (100, 10, 1, 0.1, 0.01 mM) 6 mono-species bacteria (Streptococcusmitis, Staphylococcus epidermidis, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Escherichia coli, Fusobacterium nucleatum) terms cell growth, minimal inhibitory concentration (MIC), viability. agar diffusion test, zones were only 0.01, 1 mM P. gingivalis. Growth planktonic cultures achieved 10 all species tested. viability assay, 100 potent bactericidal S. mitis, A. E. It thus concluded properties III assessed early- bacteriostatic wafers on peri-implantitis (E. coli,S. aureus, oralis, Actinomyces naeslundii, Parvimonas micra, P.gingivalis F. after 2 hours, multispecies day 3, 6. wafers, compared controls, statistically significant less viable cells mono- tests. Number colony forming units (CFUs) within significantly higher time-points evaluation coli 1. Gingipain activity ones, qPCR gingivalis comprised 15% total 6, while it remained below detection levels Sr-coated wafers. IV, impact surface roughness (turned vs moderately rough) bacterial (S. nucleatum, coli), grown consortia, vitro. Bacterial formation, well as, proteolytic reduction number nonfunctionalized times investigation Higher found non-functionalized disks ones. Sr-functionalised notable growth remined undetected time points disks. turned had slightly release rough first sustained up 15 days. Overall, data generated projects indicate exerts is worthwhile further infections.

Language: Английский

Efficacy of Linezolid-Based Hydrogel as Local Drug in Stage II Grade A Periodontitis-A Clinical Study DOI Creative Commons
Poulami Chakraborty,

Arvina Rajasekar

Annals of dental specialty, Journal Year: 2024, Volume and Issue: 12(1), P. 1 - 6

Published: Jan. 1, 2024

To prepare and characterize linezolid-based hydrogel evaluate its efficacy as local drug delivery in stage II grade A periodontitis. Linezolid-based was prepared. Hemolysis assay done at various concentrations (2%, 4%, 6%, 8%, 10%) a biocompatibility test performed by MTT Assay. Forty patients with periodontitis were involved this clinical trial. Participants split into two groups. Group (control) received only scaling root planing (SRP) an

Language: Английский

Citations

2
Antibacterial Electrodeposited Copper-Doped Calcium Phosphate Coatings for Dental Implants DOI Creative Commons

Camille Pierre,

G. Bertrand,

Iltaf Pavy

et al.

Journal of Functional Biomaterials, Journal Year: 2022, Volume and Issue: 14(1), P. 20 - 20

Published: Dec. 29, 2022

Dental implants provide a good solution for the replacement of tooth roots. However, full restoration functions relies on bone-healing period before positioning abutment and crown implant, with associated risk post-operative infection. This study aimed at developing homogeneous adherent thin calcium phosphate antibacterial coating titanium dental by electrodeposition to favor both implant osseointegration limit peri-implantitis. By combining global (XRD, FTIR-ATR, elemental titration) local (SEM, Raman spectroscopy surface thickness) characterization techniques, we determined effect time characteristics phases content mechanism its formation. The 1-min-electrodeposited CaP (thickness: 2 ± 1 μm) was mainly composed nano-needles octacalcium phosphate. We demonstrated mechanical stability after screwing unscrewing in an artificial jawbone. Then, showed that can reach high copper incorporation rate (up 27% Cu/(Cu+Ca) molar ratio) this using ionic exchange post-treatment nitrate different concentrations. biological properties (antibiofilm activity cytotoxicity) were tested vitro model mixed bacteria biofilm mimicking peri-implantitis EN 10993-5 standard (direct contact), respectively. An efficient copper-doping dose determined, providing antibiofilm property without cytotoxic side effects. processes, develop tunable thickness content.

Language: Английский

Citations

8
Inflammatory Status of Patients with Dental Implants of Different Microgeometry using ELISA: A Prospective Clinical Study DOI

Janani Gnanajothi,

Arvina Rajasekar

Journal of international oral health, Journal Year: 2024, Volume and Issue: 16(6), P. 472 - 478

Published: Nov. 1, 2024

Abstract Aims: Interleukin-1 beta (IL-1β), one of the most potent proinflammatory cytokines, plays a crucial role in bone resorption and inflammation. Its concentrations are pivotal assessing inflammatory responses around dental implants. This study aimed to conduct comparative analysis IL-1β levels peri-implant crevicular fluid (PICF) among patients with implants different microgeometries. Materials Methods: A total 78 who underwent implant placement mandibular first molar region between September 2020 January 2021 were enrolled this prospective study, following rigorous inclusion exclusion criteria. Participants categorized into three groups based on surface treatment: sandblasted acid-etched (SLA, n = 27; Group 1), SLActive ( 26; 2), TiUnite 25; 3). PICF measured by enzyme-linked immunosorbent assay (ELISA) at third month year post-implantation. Statistical analyses, including variance (ANOVA) paired t-tests, performed, P-value less than 0.05 indicating statistical significance. Results: significantly higher 3 (TiUnite implants) compared Groups 1 (SLA) 2 (SLActive) (P < 0.05). Pairwise comparisons 0.05) showed significant differences groups. Additionally, intragroup revealed increase from within each group P Conclusion: notably anodized (TiUnite), those (SLA SLActive). These findings highlight potential influence treatment inflammation resorption. Clinicians should consider as factor managing ensuring long-term success. Future studies could explore underlying mechanisms contributing elevated implants, investigate effects these treatments healing stability.

Language: Английский

Citations

1
Development, Characterization, and Antibacterial Analysis of the Selenium-Doped Bio-Glass-Collagen-Gelatin Composite Scaffold for Guided Bone Regeneration DOI Open Access

Sushma B Reddy,

P Arumugam,

Obuli G Kishore

et al.

Cureus, Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 15, 2023

Background Guided bone regeneration (GBR) is an often-used technique to aid the successful placement of dental implants in sites with deficient bone. The search for ideal GBR membrane bioactive components improving regenerative outcomes still on. In this study, a novel composite was developed using selenium-doped bio-glass, collagen, and gelatin. It further characterized surface, chemical, biocompatibility, antibacterial properties. Methodology Selenium-doped bio-glass prepared sol-gel method. fabricated equal ratio collagen gelatin mixed 1% bio-glass. solution poured obtain thin layer material which lyophilized final membrane. analyzed scanning electron microscopy, energy dispersive X-ray (EDX) analysis, attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), zebrafish cytotoxicity test, assay. Results revealed good surface roughness lamellar fibrillar arrangement minute granular cell attachment proliferation. EDX analysis presence carbon, oxygen, nitrogen as predominant trace amounts calcium, phosphorus, silica, selenium. Fourier also proved gelatin, excellent biocompatibility growth at normal rate 90% viability maintained 48, 72, 96 hours 95% 120 hours. exhibited activity against Staphylococcus aureus Escherichia coli minimal bacterial colonies. Conclusion selenium scaffold has properties along biocompatibility. Further line vivo studies should be conducted explore its role regeneration.

Language: Английский

Citations

3
Comparison of Peri-Implant Health Parameters among Obese and Non-Obese South Indian Population DOI

V. M. Kayal,

Arvina Rajasekar

Journal of Long-Term Effects of Medical Implants, Journal Year: 2023, Volume and Issue: 34(2), P. 85 - 88

Published: July 28, 2023

Implant therapy is gaining immense importance in people presenting with missing teeth due to its increased life expectancy. There a high concern regarding the link between obesity and peri-implant diseases. The aim of this study was assess health parameters among obese non-obese South Indian population. cross-sectional conducted Department Implantology, Saveetha Dental College Hospitals Chennai, India 859 patients who reported January 2022-November 2022 had minimum single implant function for at least 1 year after crown cementation. Among patients, 428 were (Group A) 431 B). Patient's data including menopausal status recorded. Clinical probing depth (PPD), clinical attachment level (CAL) radiographic parameter alveolar bone (ABL) recorded compared both groups using independent t-test. mean PPD population 2.58 &#177; 0.03 4.09 0.04, respectively. CAL 2.87 0.02 4.19 0.01, ABL 2.06 0.01 3.17 0.03, Independent <i>t</i>-test revealed there statistically significant difference (<i>P</i> &#61; 0.04), 0.05) 0.04). present suggests that individuals demonstrated depth, loss as individuals.

Language: Английский

Citations

2
Comparative Evaluation of C-Reactive Protein Levels among Peri-Implant Health and Disease Conditions DOI

K. Sreekala Priyadharsini,

Arvina Rajasekar

Journal of Long-Term Effects of Medical Implants, Journal Year: 2023, Volume and Issue: 34(3), P. 19 - 22

Published: Sept. 22, 2023

Peri-implant disease pathogenesis results in production of pro-inflammatory mediators, among which C-reactive protein (CRP) is one the acute phase reactants. The aim study was to comparative CRP levels peri-implant health and conditions. present carried out Department Implantology, Saveetha Dental College Hospitals, Chennai, India. A total 40 patients with (<i>n</i> &#61; 10), peri-mucositis early peri-implantitis 10) advanced were enrolled. Unstimulated salivary samples collected subjected latex agglutination assay for analysis. then correlated diseases. level health, mucositis, 0.18 &#177; 0.04 mg/dL, 2.05 0.61 4.14 1.82 mg/dL 6.21 1.35 respectively. There a statistically significant difference between all tested groups (ANOVA, <i>P</i> 0.03). Pearson correlation coefficient analysis revealed strong positive status. high followed by mucositis health. Also, increases severity diseases there exists

Language: Английский

Citations

2
Molecular Characterization of the glyA Gene From the Clinical Isolates of Tannerella forsythia DOI Open Access

A. Sujitha,

A. S. Smiline Girija,

Vijayashree J Priyadharsini

et al.

Cureus, Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 26, 2024

Background: The glyA gene in Tannerella forsythia is attributed for its virulence by producing the enzyme serine hydroxymethyltransferase (SHMT), which plays a vital role bacterial cell metabolism. Objectives: study thus aimed to determine frequency of from clinical strains T. isolated periodontitis patients. Materials and methods: Forty-five patients with varying degrees were included study, plaque samples collected them anaerobically processed inoculating onto sterile anaerobic blood agar plates using gaspak system, incubation at 37°C 5-7 days. DNA was extracted obtained colony, PCR performed confirm presence gene. Results: In total, 46.6% (n = 7) cases group III aggressive 15) 6.66% 1) II stage showed forsythia, among them, 57.14% 4) Conclusion: findings that may be associated pathogenesis could novel candidate future theragnostic approach combat periodontitis.

Language: Английский

Citations

0
Multiplex Real-Time Polymerase Chain Reaction Analysis of Pathogens in Peri-Implantitis and Periodontitis: A Randomized Trial DOI Creative Commons
Eun-Deok Jo

Journal of dental hygiene science, Journal Year: 2024, Volume and Issue: 24(2), P. 97 - 106

Published: June 28, 2024

Other SectionsAbstractIntroductionMaterials and MethodsResultsDiscussionAcknowledgementsConflict of interestEthical approvalFundingData availabilityReference

Language: Английский

Citations

0
Anti-biofilm activity of 445 nm and 970 nm diode laser on mixed species colonies of- aggregatibacter actinomycetemcomitans and porphyromonas gingivalis cultured on titanium discs -an in vitro study DOI

S. Suma Mohan,

Vamsi Lavu,

Supraja Ajitkumar

et al.

Lasers in Medical Science, Journal Year: 2024, Volume and Issue: 39(1)

Published: Aug. 2, 2024

Language: Английский

Citations

0
Frequency of Mutacin Gene Types in Streptococcus mutans Isolated From Oral Potentially Malignant Disorder (OPMD) Patients DOI Open Access

Srudhika Sakthivel,

A. S. Smiline Girija,

Vijayashree J Priyadharsini

et al.

Cureus, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 6, 2024

Objectives Mutacins are potent virulent factors attributing to the virulence in

Language: Английский

Citations

0