Cellular Immune Responses in Lymphoid Tissues of Broiler Chickens Experimentally Infected with Necrotic Enteritis–Producing Clostridium perfringens Strains

Avian Diseases, Journal Year: 2023, Volume and Issue: 67(2)

Published: June 29, 2023

Respuestas inmunes celulares en tejidos linfoides de pollos engorde infectados experimentalmente con cepas Clostridium perfringens productoras enteritis necrótica. Las respuestas del huésped contra (CP), el agente causante la necrótica (NE) pollos, son poco conocidas. En presente estudio, primero se analizó capacidad producción siete C. netB+ (CP5, CP18, CP26, CP64, CP67, CP68 y NCNE-1), utilizando un modelo infección experimental engorde. La evaluación las lesiones macroscópicas intestinales mostró que todas cepas, excepto CP5, podían producir necrótica, mientras CP26 CP64 produjeron relativamente más severas comparación los otros grupos. Posteriormente, evaluaron tonsilas cecales (CT), bolsa Fabricio bazo representan variaciones nivel virulencia, por ejemplo CP5 avirulenta, CP18 virulenta cepa virulenta. El análisis inmunofenotipado frecuencias macrófagos esplénicos o fueron significativamente altas controles no infectados, células T γd B grupos CP18. tenían número mayor esplénicas CD4+ CD8+ expresaban moléculas activación CD44 CD28, también una frecuencia estas activadas CP5. conjunto, estos hallazgos sugirieron celulares, incluida T, inducen selectivamente virulentas características este patógeno pueden influir resultado inmunidad

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Immunization of turkeys with Clostridium septicum alpha toxin-based recombinant subunit proteins can confer protection against experimental Clostridial dermatitis

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(4), P. e0302555 - e0302555

Published: April 29, 2024

Clostridial dermatitis (CD), caused by Clostridium septicum , is an emerging disease of increasing economic importance in turkeys. Currently, there are no effective vaccines for CD control. Here, two non-toxic domains C . alpha toxin, namely ntATX-D1 and ntATX-D2, were identified, cloned, expressed Escherichia coli as recombinant subunit proteins to investigate their use potential vaccine candidates. Experimental groups consisted a Negative control (NCx) that did not receive challenge, while the adjuvant-only Positive (PCx), immunization (D1) ntATX-D2 (D2) received challenge. Turkeys immunized subcutaneously with 100 μg protein at 7, 8 9 weeks age along oil-in-water nano-emulsion adjuvant, followed challenge 11 age. Results showed 46.2% birds PCx group died post-challenge, rate mortality D1- or D2-immunization was 13.3%. The gross histopathological lesions skin, muscle spleen severity highest group, D2-immunized had significantly lower lesion scores when compared PCx. Gene expression analysis revealed higher pro-inflammatory cytokine genes than NCx D2 these Peripheral blood cellular increased frequencies activated CD4+ and/or CD8+ cells D1 groups. Additionally, turkeys developed antigen-specific serum IgY antibodies. Collectively, findings indicate ntATX proteins, specifically can be promising candidate protecting against protection mechanisms may include downregulation -induced inflammation activation.

Language: Английский

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Characterization of immune responses and immunopathology in turkeys experimentally infected with clostridial dermatitis-producing strains of Clostridium septicum

Veterinary Immunology and Immunopathology, Journal Year: 2024, Volume and Issue: 269, P. 110717 - 110717

Published: Jan. 24, 2024

Language: Английский

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Unraveling frontiers in poultry health (part 1) – Mitigating economically important viral and bacterial diseases in commercial Chicken and Turkey production

Poultry Science, Journal Year: 2024, Volume and Issue: 103(4), P. 103500 - 103500

Published: Feb. 7, 2024

This symposium offered up-to-date perspectives on field experiences and the latest research significant viral bacterial diseases affecting poultry. A highlight was discussion use of enteroids as advanced in vitro models for exploring disease pathogenesis. Outcomes this included identifying urgent need to improve prevention control avian influenza by focusing vaccine effectiveness. In regard, efforts should focus enhancing relatedness antigen (challenge) virus strain improving immunogenicity. It also revealed that gangrenous dermatitis could be controlled through withholding or restricting administration ionophores during broiler life cycle, microscopic polymer beads (gel) based-live coccidia vaccines chicks used reduce necrotic enteritis-induced mortality. emphasized effective diagnosis re-emerging turkey (such blackhead, fowl cholera, coccidiosis) emerging such reoviral hepatitis, arthritis, Ornithobacterium rhinotracheale infection, strepticemia require complementarity between investigative approaches production Veterinarian approaches. Lastly, it determined development a variety functionally-specific would expedite delineation enteric pathogen mechanisms identification novel adjuvants.

Language: Английский

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Clostridial Dermatitis in Turkeys

Springer eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 131 - 141

Published: Jan. 1, 2024

Language: Английский

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