Gene, Journal Year: 2022, Volume and Issue: 856, P. 147129 - 147129
Published: Dec. 20, 2022
Language: Английский
Pharmaceuticals, Journal Year: 2023, Volume and Issue: 16(4), P. 586 - 586
Published: April 13, 2023
Chronic myeloid leukemia (CML) is a myeloproliferative disease characterized by the formation of BCR-ABL (breakpoint cluster region-Abelson) oncoprotein. As many patients display therapeutic resistance, development new drugs based on semisynthetic products represents potential approach for treating disease. In this study, we investigated cytotoxic activity, possible mechanism action hybrid compound betulinic acid (BA) and brosimine B in CML cell lines that are sensitive (K-562) resistant (K-562R) to imatinib, addition evaluating lower doses imatinib combination with compound. The effects compound, its apoptosis, cycle, autophagy oxidative stress were determined. was K-562 (23.57 ± 2.87 μM) K-562R (25.80 3.21 cells, synergistic effect observed when it associated imatinib. Apoptosis mediated caspase 3 9 intrinsic pathway, cycle evaluation showed arrest at G0/G1. addition, increased production reactive oxygen species induced increasing LC3II Beclin-1 mRNA levels. Results suggest causes death both imatinib-sensitive -resistant may hold as anticancer treatment against CML.
Language: Английский
Citations
8
Current Protein and Peptide Science, Journal Year: 2023, Volume and Issue: 24(10), P. 820 - 842
Published: April 19, 2023
Abstract: Nowadays, discovering an effective and safe anticancer medication is one of the major challenges. Premature death due to unidirectional toxicity conventional therapy common in cancer patients with poor health status. Plants have been used as medicine since prehistoric times, extensive research on properties various bioactive phytomolecules ongoing. Pentacyclic triterpenoids are secondary metabolites plants well-known cytotoxic chemopreventive established numerous studies. The lupane, oleanane, ursane groups these well-studied recent decades for their potential antitumor activity. This review delves into molecular machinery governing plant-derived triterpenes' efficacy. highlighted mechanisms antiproliferative activity, induction apoptosis through regulation BCL-2 BH3 family proteins, modulation inflammatory pathway, interference cell invagination inhibition metastasis. Lack solubility mostly biological solvents barrier therapeutic progress triterpenoids. also highlights some probable ways mitigate this issue help nanotechnology modification physical forms.
Language: Английский
Citations
5
Bratislavské lekárske listy/Bratislava medical journal, Journal Year: 2023, Volume and Issue: 124(02), P. 104 - 108
Published: Jan. 1, 2023
OBJECTIVES: Betulinic acid is pentacyclic triterpenoid known to exert antitumor effects by modulating many cellular pathways in various human malignancies.However, its modulatory role autophagy renal cell carcinoma remains unclear.Here, we observed how betulinic affects cells.METHODS: After treating cells with acid, determined the gene expression and protein levels of Beclin-1 ATG-5 qPCR ELISA assay observe on autophagy.RESULTS: The results demonstrated that level was low untreated metastatic adenocarcinoma ACHN increased response 25 μM 50 treatment.ATG-5 decreased primary clear CAKI-2 treated acid.In results, caused a decrease at concentration cells.CONCLUSION: In current study, it concluded may differ depending their origin these vary accordingly (Fig. 4, Ref. 40).
Language: Английский
Citations
5
Neuroscience, Journal Year: 2022, Volume and Issue: 511, P. 13 - 28
Published: Nov. 11, 2022
Language: Английский
Citations
7
Gene, Journal Year: 2022, Volume and Issue: 856, P. 147129 - 147129
Published: Dec. 20, 2022
Language: Английский
Citations
7