FTO-mediated m6A modification of QPCT promotes tumorigenesis in lung adenocarcinoma by inducing macrophage chemotaxis and M2 polarization
American Journal of Cancer Research,
Journal Year:
2025,
Volume and Issue:
15(3), P. 1036 - 1050
Published: Jan. 1, 2025
Lung
adenocarcinoma
(LUAD),
the
most
common
histologic
subtype
of
lung
cancer,
is
characterized
by
malignant
and
high
infiltrating.
Glutaminyl-peptide
cyclotransferase
(QPCT)
promotes
cancer
progression
modifying
N-terminus
chemokine
C-C
motif
ligand
2
(CCL2)
to
a
pyroglutamate
residue
stabilizing
protein.
The
role
QPCT
in
LUAD
still
unknown.
mRNA
protein
expression
were
up-regulated
clinical
specimens.
By
generating
stable
HCC44
cells
with
overexpression
A549
knockdown,
we
found
that
knockdown
notably
inhibited
cell
proliferation.
Additionally,
deletion
reduced
CCL2
contents
supernatants
phorbol
12-myristate
13-acetate-induced
THP-1
macrophage
chemotaxis
toward
tumor
or
conditioned
medium.
CD68+/CD206+
ratio
was
vitro.
Nude
mice
inoculated
parental
used
explore
functions.
results
consistent
vitro
experiments.
predicted
be
modified
N6-methyladenosine
(m6A),
performed
methylated
RNA
immunoprecipitation
PCR
confirm
this
result
cells.
m6A
demethylase
fat
mass
obesity-associated
(FTO)
positively
correlated
samples.
FTO
bound
affected
stability.
abrogated
recruitment
M2
polarization
induced
overexpression.
In
conclusion,
tumorigenesis
increasing
proportion.
This
may
due
FTO-mediated
demethylation
Language: Английский
GPR176 Is a Biomarker for Predicting Prognosis and Immune Infiltration in Stomach Adenocarcinoma
Mediators of Inflammation,
Journal Year:
2023,
Volume and Issue:
2023, P. 1 - 21
Published: April 19, 2023
Immunotherapy
based
on
immune
checkpoint
inhibitors
(ICIs)
is
considered
to
be
a
promising
treatment
for
stomach
adenocarcinoma
(STAD),
but
only
minority
of
patients
benefit
from
it.
It
believed
that
the
poor
therapeutic
efficacy
attributed
complex
tumor
microenvironment
(TIM)
STAD.
Therefore,
elucidating
specific
regulatory
mechanism
TIM
in
STAD
critical.
Previous
study
suggests
GRP176
may
involved
regulating
pace
circadian
behavior,
and
its
role
tumors
has
not
been
reported.
In
this
study,
we
first
found
GPR176
was
highly
expressed
negatively
correlated
with
patient
prognosis.
Next,
investigated
relationship
between
clinical
characteristics,
results
showed
stage
closely
related
level
GPR176.
addition,
our
further
analysis
expression
significantly
chemotherapeutic
drug
sensitivity
ICI
response.
KEGG
GO
analyses
might
stromal
remodeling
Furthermore,
analyzed
association
implication,
revealed
infiltration
various
cells.
Interestingly,
induced
conversion
while
reducing
burden
(TMB).
The
immunomodulators.
Moreover,
performed
univariate
multivariate
regression
immunomodulators
finally
obtained
4
genes
(CRCR4,
TNSF18,
PDCD1,
TGFB1).
Then,
constructed
GRP176-related
immunomodulator
prognostic
model
(GRIM)
above
genes,
which
validated
have
good
predictive
power.
Finally,
developed
nomogram
risk
score
GRIM
verified
accuracy.
These
suggested
prognosis
new
potential
target
immunotherapy
Language: Английский
Identification of metabolic biomarkers for diagnosis of epithelial ovarian cancer using internal extraction electrospray ionization mass spectrometry (iEESI-MS)
Cancer Biomarkers,
Journal Year:
2023,
Volume and Issue:
37(2), P. 67 - 84
Published: May 23, 2023
Epithelial
ovarian
cancer
(EOC)
is
the
leading
cause
of
death
from
gynecologic
malignancies.
The
poor
prognosis
EOC
mainly
due
to
its
asymptomatic
early
stage,
lack
effective
screening
methods,
and
a
late
diagnosis
in
advanced
stages
disease.This
study
investigated
metabolomic
abnormalities
epithelial
cancers.Our
developed
novel
strategy
rapidly
identify
metabolic
biomarkers
plasma
patients
using
Internal
Extraction
Electrospray
Ionization
Mass
Spectrometry
(IEESI-MS)
Liquid
Chromatography-mass
(HPLC-MS),
which
could
distinguish
differential
metabolites
between
samples
collected
98
with
cancer,
including
78
cases
original
(P),
20
self-configuration
(ZP),
as
well
60
healthy
subjects,
30
sample
(H),
(ZH),
6
blind
(B).Our
detected
880
based
on
criteria
variable
importance
projection
(VIP)
>
1,
among
26
were
selected
for
further
identification.
They
are
metabolism-related
lipids,
amino
acids,
nucleic
others.
pathways
associated
explored
by
KEGG
analysis,
comprehensive
database
that
integrates
genome,
chemistry,
system
function
information.
abnormal
identified
IEESI-MS
HPLC-MS
included
Lysophosphatidylcholine
(16:0)
[Lyso
PC
(16:0)],
L-Phenylalanine,
L-Leucine,
Phenylpyruvic
acid,
L-Tryptophan,
L-Histidine.Identifying
through
metabolomics
analyses
provide
new
valuable
potential
EOC.
Language: Английский