miR‐185 affected the EMT, cell viability, and proliferation via DNMT1/MEG3 pathway in TGF‐β1‐induced renal fibrosis DOI
Rong Xue, Yingping Li, Xiaoli Li

et al.

Cell Biology International, Journal Year: 2018, Volume and Issue: 43(10), P. 1152 - 1162

Published: Aug. 10, 2018

Abstract Kidney fibrosis is usually the final manifestation of a wide variety renal diseases. Recent years, research reported that long non‐coding RNAs (lncRNAs) played important roles in human However, role and underlying mechanisms lncRNAs kidney were complicated largely unclear. In our study, we constructed cell model HK2 cells using transforming growth factor β1 (TGF‐β1) found lncRNA maternally expressed gene 3 (MEG3) was downregulated TGF‐β1‐induced fibrosis. We then overexpressed MEG3 inhibited promotion epithelial–mesenchymal transition, viability, proliferation. Furthermore, demonstrated DNA methyltransferases 1 (DNMT1) regulated expression by altering CpGs methylation level promoter addition, further revealed miR‐185 could regulate DNMT1 thus, modulate Ultimately, study illustrated modulation miR‐185/ DNMT1/ pathway exerted summary, finding displayed novel regulatory mechanism for fibrosis, which provided new potential therapeutic target

Language: Английский

MEG3: an Oncogenic Long Non-coding RNA in Different Cancers DOI

Arwa Al-Rugeebah,

Mohammed Alanazi,

Narasimha Reddy Parine

et al.

Pathology & Oncology Research, Journal Year: 2019, Volume and Issue: 25(3), P. 859 - 874

Published: Feb. 21, 2019

Language: Английский

Citations

177
Long non‐coding RNAs in cancer: Another layer of complexity DOI
Jaqueline Carvalho de Oliveira, Luana Caroline Oliveira, Carolina Mathias

et al.

The Journal of Gene Medicine, Journal Year: 2018, Volume and Issue: 21(1)

Published: Dec. 14, 2018

We review the most well characterized long non-coding RNAs (lncRNAs) with important roles in hallmarks of cancer, additionally including lncRNAs a higher potential for clinical application. LncRNAs are transcripts larger than 200 nucleotides length that do not appear to have protein-coding potential, although some those may produce small functional peptides. These attracted significant attention from researchers as result their role genetic regulation, epigenetic, transcriptional and post-transcriptional being involved numerous biological processes, associated multifactorial diseases, tumorigenesis. The cancer include sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis activating invasion/metastasis. Additionally, genome instability, inflammation, reprogramming energy metabolism immune destruction implicated all cancer. Based on great number studies describing diverse aspects tumor types, essential potentially features cells show utility diagnostic prognostic markers, exemplified by PCA3 lncRNA detection prostate diagnosis.

Language: Английский

Citations

108
MEG8 long noncoding RNA contributes to epigenetic progression of the epithelial-mesenchymal transition of lung and pancreatic cancer cells DOI Creative Commons
Minoru Terashima, Akihiko Ishimura, Sasithorn Wanna-udom

et al.

Journal of Biological Chemistry, Journal Year: 2018, Volume and Issue: 293(47), P. 18016 - 18030

Published: Sept. 27, 2018

Language: Английский

Citations

98
LncRNA-MEG3 inhibits activation of hepatic stellate cells through SMO protein and miR-212 DOI Creative Commons
Fujun Yu, Wujun Geng, Peihong Dong

et al.

Cell Death and Disease, Journal Year: 2018, Volume and Issue: 9(10)

Published: Oct. 3, 2018

Abstract Activation of hepatic stellate cells (HSCs), a pivotal event in liver fibrosis, is considered as an epithelial–mesenchymal transition (EMT) process. Deregulation long noncoding RNAs (lncRNAs) has been reported to be involved series human diseases. LncRNA-maternally expressed gene 3 (MEG3) functions tumor suppressor cancers and shown play vital role EMT However, the biological MEG3 fibrosis largely unknown. In this study, was reduced vivo vitro during fibrosis. Restoring expression led suppression with reduction α-SMA type I collagen. Notably, overexpression inhibited HSC activation through EMT, associated increase epithelial markers mesenchymal markers. Further studies showed that Hedgehog (Hh) pathway-mediated process effects on activation. Smoothened (SMO) member Hh pathway. Using bioinformatic analysis, interaction between SMO protein predicted. This confirmed by results RNA immunoprecipitation deletion-mapping analysis. Furthermore, target microRNA-212 (miR-212). miR-212 partly responsible for Interestingly, also chronic hepatitis B (CHB) patients when compared healthy controls. negatively correlated stage CHB patients. conclusion, we demonstrate inhibits Hh-mediated via miR-212.

Language: Английский

Citations

86
LncRNA MEG3 targeting miR-424-5p via MAPK signaling pathway mediates neuronal apoptosis in ischemic stroke DOI Creative Commons

Yanxiao Xiang,

Yayun Zhang,

Yanni Xia

et al.

Aging, Journal Year: 2020, Volume and Issue: 12(4), P. 3156 - 3174

Published: Feb. 16, 2020

Emerging evidence suggests that long non-coding RNAs (lncRNAs) are significant regulators in the pathological process of ischemic stroke (IS).However, little is known about lncRNAs and their roles IS.In this study, we aimed to screen out differentially expressed revealed underlying mechanisms IS.The results bioinformatic analysis showed lncRNA MEG3 Sema3A were overexpressed IS samples, while miR-424-5p was lower-expressed.Correlation between MEG3/miR-424-5p, miR-424-5p/Sema3A predicted with miRanda TargetScan, verified by dual luciferase assay.Inhibition remarkably increased expression decreased Sema3A, which also led an cell viability cellular apoptosis oxygenglucose deprivation reoxygenation (OGD/R) model, as well activated MAPK signaling pathways.Consistently, upregulated MCAO mice, knockdown reduced infarct volume improved neurobehavioral outcomes rats following MCAO.In conclusion, it demonstrated accelerated suppressing miR-424-5p, targeted pathway.These might provide useful information for exploring potential therapeutic targets IS.

Language: Английский

Citations

82
Long non-coding RNAs in gastric cancer: New emerging biological functions and therapeutic implications DOI Creative Commons

Huidan Tan,

Shouyue Zhang, Jin Zhang

et al.

Theranostics, Journal Year: 2020, Volume and Issue: 10(19), P. 8880 - 8902

Published: Jan. 1, 2020

Gastric cancer (GC) is currently the fourth most common malignancy and third leading cause of cancer-related deaths worldwide. Long non-coding RNAs (lncRNAs), transcriptional products with more than 200 nucleotides, are not as well-characterized protein-coding RNAs. Accumulating evidence has recently revealed that maladjustments diverse lncRNAs may play key roles in multiple genetic epigenetic phenomena GC, affecting all aspects cellular homeostasis, such proliferation, migration, stemness. However, full extent their functionality remains to be clarified. Considering lack viable biomarkers therapeutic targets, future research should focused on unravelling intricate relationships between GC can translated from bench clinic. Here, we summarized state-of-the-art advances biological functions further discuss potential diagnostic roles. We aim shed light interrelationships respect applications. With better understanding these relationships, development will exploitable, promising new strategies developed for prevention treatment GC.

Language: Английский

Citations

81
The Role of Long Non-Coding RNAs in Hepatocarcinogenesis DOI Open Access
Manuela Lanzafame,

Gaia Bianco,

Luigi Terracciano

et al.

International Journal of Molecular Sciences, Journal Year: 2018, Volume and Issue: 19(3), P. 682 - 682

Published: Feb. 28, 2018

Whole-transcriptome analyses have revealed that a large proportion of the human genome is transcribed in non-protein-coding transcripts, designated as long non-coding RNAs (lncRNAs). Rather than being “transcriptional noise”, increasing evidence indicates lncRNAs are key players regulation many biological processes, including transcription, post-translational modification and inhibition chromatin remodeling. Indeed, widely dysregulated cancers, hepatocellular carcinoma (HCC). Functional studies beginning to provide insights into role oncogenic tumor suppressive cell proliferation motility, well metastatic potential HCC. A better understanding molecular mechanisms complex network interactions which involved could reveal novel diagnostic prognostic biomarkers. Crucially, it may therapeutic opportunities add currently limited number options for HCC patients. In this review, we summarize current status field, with focus on best characterized

Language: Английский

Citations

81
Role of lncRNAs in aging and age‐related diseases DOI Creative Commons
Jieyu He, Chao Tu, You‐Shuo Liu

et al.

Aging Medicine, Journal Year: 2018, Volume and Issue: 1(2), P. 158 - 175

Published: July 30, 2018

Abstract Aging is progressive physiological degeneration and consequently declined function, which linked to senescence on both cellular organ levels. Accumulating studies indicate that long noncoding RNA s (lnc s) play important roles in at all levels—transcriptional, post‐transcriptional, translational, post‐translational. Understanding the molecular mechanism of lnc underlying could facilitate interpretation intervention aging age‐related diseases. In this review, we describe categories known novel have been involved progression senescence. We also identify implicated diseases arising from age‐driven or dysfunction some representative organs systems (brains, liver, muscle, cardiovascular system, bone pancreatic islets, immune system). Improved comprehension process levels, cell organismal, may provide new insights into amelioration pathologies prolonged healthspan.

Language: Английский

Citations

76
LncRNA MEG3 inhibits rheumatoid arthritis through miR‐141 and inactivation of AKT/mTOR signalling pathway DOI Creative Commons
Guoqing Li, Ying Liu,

Fanru Meng

et al.

Journal of Cellular and Molecular Medicine, Journal Year: 2019, Volume and Issue: 23(10), P. 7116 - 7120

Published: Aug. 14, 2019

Rheumatoid arthritis (RA) is a chronic inflammation mediated by autoimmune responses. MEG3, kind of long noncoding RNA (lncRNA), participates in cell proliferation cancer tissues. However, the correlation between MEG3 and RA yet unclear. Therefore, to clarify how works RA, we performed series experiments using samples. We found that was downregulated fibroblast-like synoviocytes patients (RA-FLS), comparison with healthy subjects. also down-regulated evidently lipopolysaccharide (LPS)-treated chondrocyte. As part our experiments, overexpressed chondrocyte transfection lentivirus containing sequences encoding MEG3. In addition, presence LPS, reductions were identified not only proliferation, but generation interleukin-23 (IL-23), which, however reversed (containing MEG3-encoding sequences)-transfected chondrocytes. Up-regulated resulted an increase level Ki67. Moreover, negatively correlated miR-141, miR-141 up-regulated LPS-treated Inhibitory effects overexpression, mentioned above, partially abolished miR-141. Further, animal experiment showed inhibitory effect overexpression on AKT/mTOR signaling pathway. In-vivoexperiments facilitated inhibited inflammation. summary, protective role proved be exerted rate which might correlate regulatory signal pathway, suggesting holds great promise as therapeutic strategy for RA.

Language: Английский

Citations

75
Noncoding RNAs in Nonalcoholic Fatty Liver Disease: Potential Diagnosis and Prognosis Biomarkers DOI Open Access
Olfa Khalifa, Khaoula Errafii‬, Nayla S. Al-Akl

et al.

Disease Markers, Journal Year: 2020, Volume and Issue: 2020, P. 1 - 16

Published: Aug. 27, 2020

Nonalcoholic fatty liver disease (NAFLD) is currently the most common chronic worldwide in part due to concomitant obesity pandemic and insulin resistance (IR). It increasingly becoming evident that NAFLD a affecting numerous extrahepatic vital organs regulatory pathways. The molecular mechanisms underlying nonalcoholic steatosis formation are poorly understood, little information available on pathways responsible for progressive hepatocellular damage follows lipid accumulation. Recently, much research has focused identification of epigenetic modifications contribute pathogenesis. Noncoding RNAs (ncRNAs) one such factors could be implicated development progression. In this review, we summarize current knowledge genetic potentially disease. Particular emphasis will put contribution microRNAs (miRNAs), long noncoding (lncRNAs), circular (circRNAs) pathophysiology as well their potential use therapeutic targets or markers prediction progression

Language: Английский

Citations

55