LUCAT1-Mediated Competing Endogenous RNA (ceRNA) Network in Triple-Negative Breast Cancer DOI Creative Commons
Deepak Verma, Sumit Siddharth, Ashutosh S. Yende

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(22), P. 1918 - 1918

Published: Nov. 19, 2024

Breast cancer is a heterogeneous disease comprising multiple molecularly distinct subtypes with varied prevalence, prognostics, and treatment strategies. Among them, triple-negative breast cancer, though the least prevalent, most aggressive subtype, limited therapeutic options. Recent emergence of competing endogenous RNA (ceRNA) networks has highlighted how long noncoding RNAs (lncRNAs), microRNAs (miRs), mRNA orchestrate complex interplay meticulously modulating functionality. Focusing on TNBC, this study aimed to construct ceRNA network using differentially expressed lncRNAs, miRs, mRNAs. We queried lncRNAs (DElncRNAs) between TNBC luminal samples found 389 upregulated 386 downregulated including novel transcripts in TNBC. DElncRNAs were further evaluated for their clinical, functional, mechanistic relevance TNBCs lnc2cancer 3.0 database, which presented LUCAT1 (lung cancer-associated transcript 1) as putative node. Next, (lncRNA-miRNA-mRNA) was established. Several miRNA-mRNA connections implicated regulating stemness (LUCAT1-miR-375-Yap1, LUCAT1-miR181-5p-Wnt, LUCAT1-miR-199a-5p-ZEB1), apoptosis (LUCAT1-miR-181c-5p-Bcl2), drug efflux (LUCAT1-miR-200c-ABCB1, LRP1, MRP5, MDR1), sheddase activities (LUCAT1-miR-493-5p-ADAM10) identified, indicating an intricate regulatory mechanism Indeed, silencing led mitigated cell growth, migration, stem-like features This work sheds light implies its involvement growth progression.

Language: Английский

Dysregulation of transposable elements and PIWI-interacting RNAs in myelodysplastic neoplasms DOI Creative Commons
Zdeněk Krejčík, Dávid Kundrát, Jǐŕı Kléma

et al.

Biomarker Research, Journal Year: 2025, Volume and Issue: 13(1)

Published: Jan. 23, 2025

Abstract Background Myelodysplastic neoplasms (MDS) are heterogeneous hematopoietic disorders characterized by ineffective hematopoiesis and genome instability. Mobilization of transposable elements (TEs) is an important source instability leading to oncogenesis, whereas small PIWI-interacting RNAs (piRNAs) act as cellular suppressors TEs. However, the roles TEs piRNAs in MDS remain unclear. Methods In this study, we examined TE piRNA expression through parallel RNA sequencing CD34+ stem cells from patients. Results Comparative analysis between control samples revealed several significantly dysregulated molecules. significant differences were observed lower-risk (LR-MDS) higher-risk (HR-MDS) samples. HR-MDS, found inverse correlation decreased levels increased these associated with patient outcomes. Importantly, upregulation PIWIL2 , which encodes a key factor pathway, independently predicted poor prognosis patients, underscoring its potential valuable disease marker. Furthermore, pathway data that dysregulation TE‒piRNA axis linked suppression processes related energy metabolism, cell cycle, immune response, suggesting disruptions affect activity. Conclusions Our findings demonstrate HR-MDS highlighting their role progression indicating level promising molecular marker for prognosis. Graphical

Language: Английский

Citations

1
Prognostic and diagnostic value of circRNA expression in cervical cancer: a meta analysis DOI Creative Commons
Yi Xu, Changxia Li,

Li-fang Cheng

et al.

Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 14

Published: Jan. 13, 2025

Introduction Cervical cancer (CC) is a highly prevalent malignancy of the reproductive system. This study aimed to methodically assess function circular RNAs (circRNAs) as possible indicators CC, with specific emphasis on their usefulness in identification, prediction, and correlation clinicopathological elements. Methods A comprehensive literature search was conducted using databases such PubMed, Cochrane Library, Web Science, Embase, China National Knowledge Infrastructure (CNKI). The latest data were extracted May 3 rd , 2024. diagnostic potential circRNA expression evaluated range metrics including sensitivity, specificity, area under receiver operating characteristic curve (AUC). importance circRNAs further terms clinical relevance, pathological features, prognostic value pooled odds ratios (ORs) hazard (HRs). Results meta-analysis included 27 studies, which categorised based applications (n=3), correlations (n=15), evaluations (n=23). Elevated levels oncogenic significantly associated poor indicators, tumour size (odds ratio [OR] = 0.425, 95% confidence interval [CI]: 0.267–0.676), International Federation Gynaecology Obstetrics (FIGO) stage (OR 0.315, CI: 0.224–0.443), lymph node metastasis 2.975, 1.816–4.872). upregulation also identified predictor worse survival outcomes, (HR) 2.13 (95% 1.73–2.62, P < 0.001). downregulation tumour-suppressor properties similarly parameters, 0.310, 0.102–0.941), FIGO 0.231, 0.101–0.527), 2.430, 1.156–5.110), indicative worsened prognosis (HR 2.20, 1.03–4.70, 0.042). In value, (AUC) calculated be 0.85, 0.83, 0.91, respectively. Conclusion results our indicate that have serve promising biomarkers for CC diagnosis, prognosis, clinicopathology. Systematic review registration https://www.crd.york.ac.uk/PROSPERO/ identifier CRD42024544997.

Language: Английский

Citations

0
Regulation of immune-mediated chemoresistance in cancer by lncRNAs: an in-depth review of signaling pathways DOI
Saade Abdalkareem Jasim, Farag M. A. Altalbawy, Subasini Uthirapathy

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: April 9, 2025

Language: Английский

Citations

0
A novel role of miR-223-3p in reducing NLRP3-mediated inflammation and deep vein thrombosis in a mouse model DOI Creative Commons
Ji Luo, Lei Zheng, Hongyu Zheng

et al.

Science Progress, Journal Year: 2025, Volume and Issue: 108(2)

Published: April 1, 2025

Objective Deep vein thrombosis (DVT) is a global health issue caused by abnormal clotting in deep veins, which can lead to serious complications such as pulmonary embolism. This study the first validate regulatory effect of miR-223-3p on NLRP3 inflammasome mouse model DVT, expanding its potential therapeutic value venous thrombosis-associated inflammation. Methods MicroRNA sequencing and quantitative real-time polymerase chain reaction (qRT–PCR) were conducted assess miRNA expression DVT model. The downstream target miR-223-3p, NLRP3, was identified using prediction databases validated qRT–PCR. Human umbilical endothelial cells (HUVECs) used explore functional relationship between Nlrp3. Results Nlrp3 significantly increased walls mice with DVT. tail injection agomiR-223-3p reduced thrombus formation downregulated Nlrp3, interleukin 6 (Il-6), 1 beta (IL-1beta) Icam-1. In vitro, overexpression Il-6, IL-1beta ICAM-1, whereas antagonized these effects. Additionally, enhanced viability migration LPS-stimulated HUVECs reducing expression. Conclusions Our findings suggest that may play role alleviating inflammation burden downregulating expression, supporting for

Language: Английский

Citations

0
LUCAT1-Mediated Competing Endogenous RNA (ceRNA) Network in Triple-Negative Breast Cancer DOI Creative Commons
Deepak Verma, Sumit Siddharth, Ashutosh S. Yende

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(22), P. 1918 - 1918

Published: Nov. 19, 2024

Breast cancer is a heterogeneous disease comprising multiple molecularly distinct subtypes with varied prevalence, prognostics, and treatment strategies. Among them, triple-negative breast cancer, though the least prevalent, most aggressive subtype, limited therapeutic options. Recent emergence of competing endogenous RNA (ceRNA) networks has highlighted how long noncoding RNAs (lncRNAs), microRNAs (miRs), mRNA orchestrate complex interplay meticulously modulating functionality. Focusing on TNBC, this study aimed to construct ceRNA network using differentially expressed lncRNAs, miRs, mRNAs. We queried lncRNAs (DElncRNAs) between TNBC luminal samples found 389 upregulated 386 downregulated including novel transcripts in TNBC. DElncRNAs were further evaluated for their clinical, functional, mechanistic relevance TNBCs lnc2cancer 3.0 database, which presented LUCAT1 (lung cancer-associated transcript 1) as putative node. Next, (lncRNA-miRNA-mRNA) was established. Several miRNA-mRNA connections implicated regulating stemness (LUCAT1-miR-375-Yap1, LUCAT1-miR181-5p-Wnt, LUCAT1-miR-199a-5p-ZEB1), apoptosis (LUCAT1-miR-181c-5p-Bcl2), drug efflux (LUCAT1-miR-200c-ABCB1, LRP1, MRP5, MDR1), sheddase activities (LUCAT1-miR-493-5p-ADAM10) identified, indicating an intricate regulatory mechanism Indeed, silencing led mitigated cell growth, migration, stem-like features This work sheds light implies its involvement growth progression.

Language: Английский

Citations

0