Knockout of cryptochrome 1 disrupts circadian rhythm and photoperiodic diapause induction in the silkworm, Bombyx mori

Insect Biochemistry and Molecular Biology, Journal Year: 2024, Volume and Issue: 172, P. 104153 - 104153

Published: July 2, 2024

Most insects enter diapause, a state of physiological dormancy crucial for enduring harsh seasons, with photoperiod serving as the primary cue its induction, ensuring proper seasonal timing process. Although involvement circadian clock in photoperiodic time measurement has been demonstrated through knockdown or knockout genes, gene cryptochrome 1 (cry1), which functions photoreceptor implicated photoentrainment across various insect species, remains unclear. In bivoltine strains silkworm, Bombyx mori, embryonic diapause is maternally controlled and affected by environmental conditions experienced mother moths during larval stages. Previous research highlighted role core including period (per), timeless (tim), Clock (Clk) cycle (cyc), induction B. mori. this study, we focused on cry1 mori photoperiodism. Phylogenetic analysis conserved domain identification confirmed presence both Drosophila-type cry (cry1) mammalian-type (cry2) genes genome, akin to other lepidopterans. Temporal expression revealed higher photophase lower scotophase, knockouts (per, tim, Clk cyc) disrupting temporal pattern. Using CRISPR/Cas9-mediated genome editing, established strain p50T, exhibiting clear photoperiodism wild-type displayed rhythm eclosion under continuous darkness, exhibited arrhythmic eclosion, implicating feedback loop governing behavior rhythms. Females failed control stages, mirroring phenotype individuals reared constant indicating that CRY1 contributes photoreceptor. Furthermore, stage was abolished cry1/tim double-knockout strain, suggesting photic information received relayed clock. Overall, study represents first evidence photoperiodism, specifically induction.

Language: Английский

Photoperiodism, Insects

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский