Transcriptome profiling revealed multiple genes and ECM-receptor interaction pathways that may be associated with breast cancer

Cellular & Molecular Biology Letters, Journal Year: 2019, Volume and Issue: 24(1)

Published: June 6, 2019

Exploration of the genes with abnormal expression during development breast cancer is essential to provide a deeper understanding mechanisms involved. Transcriptome sequencing and bioinformatics analysis invasive ductal carcinoma paracancerous tissues from same patient were performed identify key signaling pathways related development.Samples tumor tissue obtained 6 patients. Sequencing used Illumina HiSeq platform. All. Only perfectly matched clean reads mapped reference genome database, further analyzed annotated based on information. Differentially expressed (DEGs) identified using DESeq R package (1.10.1) DEGSeq (1.12.0). Using KOBAS software execute KEGG analyses, enriched DEGs involved in occurrence determined. Subsequently, quantitative real time PCR was verify accuracy profile RNA-seq result explore patterns novel cancer-related 8 different clinical individuals.The transcriptomic results showed 937 DEGs, including 487 upregulated 450 downregulated specimens. Further gene captured 252 (201 51 upregulated) that differential pattern all libraries. Finally, (CST2, DRP2, CLEC5A, SCD, KIAA1211, DTL) (STAC2, BTNL9, CA4, CD300LG, GPIHBP1 PIGR), confirmed comparison revealed various pathway changes, 20 21 enrichment pathways. The extracellular matrix-receptor (ECM-receptor) interaction most pathway: this THBS family, collagen fibronectin. These ECM-receptor may perform important roles cancer.Several potential captured, 7 76 not found other studies. are cell proliferation, movement adhesion. They be for research into mechanisms, particularly CST2 CA4. A pathway, signal also as possibly cancer.

Language: Английский

---

MYBL2 amplification in breast cancer: Molecular mechanisms and therapeutic potential

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Journal Year: 2020, Volume and Issue: 1874(2), P. 188407 - 188407

Published: Aug. 25, 2020

Language: Английский

---

Cathepsin V: Molecular characteristics and significance in health and disease

Molecular Aspects of Medicine, Journal Year: 2022, Volume and Issue: 88, P. 101086 - 101086

Published: March 16, 2022

Language: Английский

---

The biology of SCUBE

Journal of Biomedical Science, Journal Year: 2023, Volume and Issue: 30(1)

Published: May 26, 2023

Abstract The SCUBE [Signal peptide-Complement C1r/C1s, Uegf, Bmp1 (CUB)-Epithelial growth factor domain-containing protein] family consists of three proteins in vertebrates, SCUBE1, 2 and 3, which are highly conserved zebrafish, mice humans. Each gene encodes a polypeptide approximately 1000 amino acids that is organized into five modular domains: (1) an N-terminal signal peptide sequence, (2) nine tandem epidermal (EGF)-like repeats, (3) large spacer region, (4) cysteine-rich (CR) motifs, (5) CUB domain at the C-terminus. Murine Scube genes expressed individually or combination during development various tissues, including those central nervous system axial skeleton. cDNAs human orthologs were originally cloned from vascular endothelial cells, but expression has also been found platelets, mammary ductal epithelium osteoblasts. Both soluble membrane-associated SCUBEs have shown to play important roles physiology pathology. For instance, upregulation reported acute myeloid leukemia, breast cancer lung cancer. In addition, SCUBE1 released activated platelets can be used as clinical biomarker for coronary syndrome ischemic stroke. Soluble SCUBE2 enhances distal signaling by facilitating secretion dual-lipidated hedgehog nearby ligand-producing cells paracrine manner. Interestingly, regions CR motifs increase enable binding cell surfaces via electrostatic glycan-lectin interactions. As such, function coreceptors enhance activity serine/threonine kinase tyrosine receptors. example, SCUBE3 functions coreceptor promotes bone morphogenesis. humans, mutations linked abnormalities differentiation both bones teeth. addition studies on function, experimental results genetically modified mouse models yielded insights field systems biology. this review, we highlight novel molecular discoveries critical directions future research context cancer, skeletal disease cardiovascular disease.

Language: Английский

---

Genetic risk factors for cancer-related cognitive impairment: a systematic review

Acta Oncologica, Journal Year: 2019, Volume and Issue: 58(5), P. 537 - 547

Published: March 1, 2019

Background: Cancer-related cognitive impairment (CRCI) is a commonly reported complaint among non-CNS cancer patients. Even subtle CRCI may have detrimental effects on quality of life and identifying patients at increased risk for to improve survivorship care important. In the present paper, we systematically reviewed available studies possible genetic factors developing CRCI.Methods: Keyword-based systematic searches were undertaken 24 July 2018 in PubMed, Web Science, The Cochrane Library, CINAHL. Three authors independently evaluated full-texts identified papers excluded with registration reasons. Seventeen reporting results from 14 independent samples included review. Two assessed studies. review was preregistered PROSPERO (CRD42018107689).Results: Ten investigated apolipoprotein E (APOE), four that carrying least one allele (APOE4 (ε4)) associated CRCI, while six found no association. remaining variants located in: COMT, DNA repair genes, five oxidative stress 22 genes related breast phenotype, GNB3. No associations between coding interleukin-6 (IL6), tumor necrosis factor alpha (TNF), interleukin 1 beta (IL1B), brain-derived neurotropic (BDNF). With exception APOE, had only been or two each.Conclusions: Overall, evidence limited. While some research suggests role ε4 allele, literature generally inconsistent, currently does not allow clear-cut conclusions regarding development CRCI. Larger investigating additional are needed uncover

Language: Английский

---

Embracing the complexity: Older adults with cancer-related cognitive decline—A Young International Society of Geriatric Oncology position paper

Journal of Geriatric Oncology, Journal Year: 2019, Volume and Issue: 11(2), P. 237 - 243

Published: Oct. 14, 2019

Language: Английский

---

Variants of the Progesterone Receptor Gene as Modulators of Risk for Idiopathic Spontaneous Premature Birth

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(4), P. 1606 - 1606

Published: Feb. 13, 2025

Premature birth (PTB) is the most common cause of perinatal mortality and morbidity. We performed a case-control study to determine whether two selected single-nucleotide polymorphisms (SNPs) progesterone receptor gene (PGR) (rs4754732 rs653752) play role in modulation risk for spontaneous PTB. This included 400 mothers (199 with premature delivery 201 term delivery) newborns (201 term-born 199 premature-born) European descent. Genotyping was an ABI PRISM 7500 SDS using TaqMan SNP genotyping assays. found no statistically significant difference distribution genotypes allele frequencies between prematurely born at either investigated SNP. There groups extremely early PTB compared group births. Potential association mothers' C rs653752 lower odds (p = 0.03; ratio 1.36; 95% confidence interval 1.02-1.81; Chi-square test), CC genotype recessive inheritance model general 0.02; 0.54; 0.32-0.91; test) late 0.005, 0.45, 0.23-0.79; were found. It also that who carriers haplotype T-G combination rs4754732 1.5 times more likely have PTB, even after correcting p-value multiple comparisons 0.008; 1.59; 1.13-2.24, test). Further research on larger number subjects these other PGR SNPs will be needed order confirm presented results.

Language: Английский

---

Novel candidate biomarkers of origin recognition complex 1, 5 and 6 for survival surveillance in patients with hepatocellular carcinoma

Journal of Cancer, Journal Year: 2020, Volume and Issue: 11(7), P. 1869 - 1882

Published: Jan. 1, 2020

Background: Hepatocellular carcinoma (HCC) has high morbidity and mortality lacks effective biomarkers for early diagnosis survival surveillance.Origin recognition complex (ORC), consisting of ORC1-6 isoforms, was examined to assess the potential significance ORC isoforms HCC prognosis.Methods: Oncomine Gene Expression Profiling Interactive Analysis (GEPIA) databases were used examine differential isoform expression, stage-specific calculate Pearson correlations perform analysis.A human protein atlas database utilized evaluate expression ORCs in liver tissue.The cBioPortal mutations HCC.Cytoscape software employed construct gene ontologies, metabolic pathways gene-gene interaction networks.Results: Differential analysis indicated that ORC1 ORC3-6 highly expressed tumor tissues GEPIA databases, while ORC2 not.All showed positive statistically significant with each other (all P<0.001).ORC1-2 ORC4-6 expressions associated disease stages I-IV P<0.05), but ORC3 not.Survival found overall (OS), ORC1-3 ORC5-6 recurrence-free (RFS; all P<0.05).In addition, low these genes consistently better prognosis compared expression.Protein revealed normal tissues, whereas not.Enrichment DNA process, sequence-specific binding involved replication, cell cycle, E2F-enabled inhibition pre-replication formation G1/S transition.Conclusions: Differentially ORC1, 5 6 are candidate prediction recurrence surveillance HCC.

Language: Английский

---

Impact of matrix metalloproteinase-11 gene polymorphisms upon the development and progression of hepatocellular carcinoma

International Journal of Medical Sciences, Journal Year: 2018, Volume and Issue: 15(6), P. 653 - 658

Published: Jan. 1, 2018

Hepatocellular carcinoma (HCC) is a liver malignancy and major cause of cancer mortality worldwide.Matrix metalloproteinase-11 (MMP-11), also known as stromelysin-3, plays critical role during tumor migration, invasion metastasis.Here, we report on the association between five single nucleotide polymorphisms (SNPs) -rs738791, rs2267029, rs738792, rs28382575, rs131451 -of MMP-11 gene HCC susceptibility, well clinical outcomes, in 293 patients with 586 cancer-free controls.We found that carriers CT+TT allele rs738791 variant were at greater risk compared wild-type (CC) carriers.Moreover, least one C (C/T+C/C genotype) SNP rs738792 likely to progress Child-Pugh B or grade, while individuals rs28382575 higher developing stage III/IV disease, large tumors lymph node metastasis.We believe genetic variations may help predict early-stage act reliable biomarkers for progression.

Language: Английский

---

Diaphanous-related formin-3 overexpression inhibits the migration and invasion of triple-negative breast cancer by inhibiting RhoA-GTP expression

Biomedicine & Pharmacotherapy, Journal Year: 2017, Volume and Issue: 94, P. 439 - 445

Published: Aug. 3, 2017

Language: Английский

---