Cited by Advancements, challenges, and future perspectives in developing feline herpesvirus 1 as a vaccine vector

Advances in Stem Cell Therapy for Huntington’s Disease: A Comprehensive Literature Review DOI

Siddharth Shah, Hadeel M. Mansour, Brandon Lucke‐Wold

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(1), P. 42 - 42

Published: Jan. 3, 2025

Huntington’s disease (HD) is an inherited neurodegenerative characterized by uncontrolled movements, emotional disturbances, and progressive cognitive impairment. It estimated to affect 4.3 10.6 per 100,000 people worldwide, the mean prevalence rate among all published studies, reviews, genetic HD registries 5.7 100,000. A key feature of loss striatal neurons cortical atrophy. Although there no cure at present, discovery gene causing has brought us into a new DNA era therapeutic advances for several neurological disorders. PubMed was systematically searched using three search strings: ‘“Huntington disease” + “stem cell”’, ‘”Huntington Mesenchymal stromal cell’, “induced pluripotent stem cell”’. For each string, results were categorized based on cell type, papers that included clinical analysis as well. The data extracted up 2024. We did not include other databases in our have comparable systematic review literature topic. collected analyzed used critical interpretation present review. Data are presented chronologically studies published. Therapeutic strategies cells drawn lot interest possible therapies. Recent research indicates NSCs been most often utilized type treating HD. generated from variety sources, including patients’ somatic brain itself. There strong evidence supporting transplantation or their derivatives animal models, even if stem-cell-based preclinical trials still early stages. Current treatment only aims relieving symptoms rather than pathogenesis disease. models shown promise improving motor functions, therapy faces many challenges disadvantages immunosuppression immunorejection well ethical, technical, safety concerns. Further required definitive conclusion.

Language: Английский

Citations

CRISPR-Cas9 Targeting PCSK9: A Promising Therapeutic Approach for Atherosclerosis DOI

Bin Gu, Man Li, Dan Li

et al.

Journal of Cardiovascular Translational Research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

Language: Английский

Citations

Emerging applications of gene editing technologies for the development of climate-resilient crops DOI

R. L. Chavhan,

Siddhant Gahininath Jaybhaye,

V. R. Hinge

et al.

Frontiers in Genome Editing, Journal Year: 2025, Volume and Issue: 7

Published: March 10, 2025

Climate change threatens global crop yield and food security due to rising temperatures, erratic rainfall, increased abiotic stresses like drought, heat, salinity. Gene editing technologies, including CRISPR/Cas9, base editors, prime offer precise tools for enhancing resilience. This review explores the mechanisms of these technologies their applications in developing climate-resilient crops address future challenges. While CRISPR/enables targeted modifications plant DNA, editors allow direct conversion without inducing double-stranded breaks, enable insertions, deletions, substitutions. By understanding manipulating key regulator genes involved stress responses, such as DREB, HSP, SOS, ERECTA, HsfA1, NHX; tolerance can be enhanced against salt stress. improve traits related root development, water use efficiency, response pathways, heat shock response, photosynthesis, membrane stability, ion homeostasis, osmotic adjustment, oxidative response. Advancements gene integration with genomics, phenomics, artificial intelligence (AI)/machine learning (ML) hold great promise. However, challenges off-target effects, delivery methods, regulatory barriers must addressed. highlights potential develop crops, contributing sustainable agriculture.

Language: Английский

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Genome Editing VEGFA Prevents Corneal Neovascularization In Vivo DOI

Zhenhai Zeng,

Siheng Li,

Xiuhong Ye

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(25)

Published: April 6, 2024

Abstract Corneal neovascularization (CNV) is a common clinical finding seen in range of eye diseases. Current therapeutic approaches to treat corneal angiogenesis, which vascular endothelial growth factor (VEGF) A plays central role, can cause variety adverse side effects. The technology Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 edit VEGFA gene suppress its expression. CRISPR offers novel opportunity CNV. This study shows that depletion with CRISPR/Cas9 system inhibits proliferation, migration, and tube formation human umbilical vein cells (HUVECs) vitro. Importantly, subconjunctival injection this dual AAV‐SpCas9/sgRNA‐ demonstrated blocks suture‐induced expression VEGFA, CD31, α‐smooth muscle actin as well mice. has established strong foundation for the treatment via editing approach first time.

Language: Английский

Citations

CRISPR/Cas9 Technology for Enhancing Desirable Traits of Fish Species in Aquaculture DOI

Minli Zhu,

Sahr Lamin Sumana, Abdullateef Mukhtar Muhammad

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9299 - 9299

Published: Aug. 27, 2024

Aquaculture, the world's fastest-growing food production sector, is critical for addressing security concerns because of its potential to deliver high-quality, nutrient-rich supplies by 2050. This review assesses effectiveness CRISPR/Cas9 genome editing technology in enhancing desirable traits fish species, including growth rates, muscle quality, disease resistance, pigmentation, and more. It also focuses on allowing precise targeted modifications DNA improve characteristics. Many studies have reported successful applications CRISPR/Cas9, such as knocking out reproductive genes control reproduction sex determination, feed conversion efficiency, reducing off-target effects. Additionally, this has contributed environmental sustainability nitrogen-rich waste improving nutritional composition fish. However, acceptance modified public consumers hindered regarding perception, ecological impacts, regulatory frameworks. To gain approval consumer confidence, clear communication about process, well data safety considerations genetically fish, are essential. paper discusses these challenges, provides possible solutions, recommends future research integration into sustainable aquaculture practices, focusing responsible management enable creation disease-resistant strains. In conclusion, highlights transformative traits, while considering challenges ethical associated with practices aquaculture.

Language: Английский

Citations

Recent advances in engineering fast-growing cyanobacterial species for enhanced CO2 fixation DOI

David Kim, José Ángel Moreno-Cabezuelo,

Eduardo Nicolas Schulz

et al.

Frontiers in Climate, Journal Year: 2024, Volume and Issue: 6

Published: June 17, 2024

Atmospheric CO 2 removal (CDR) is a fundamentally endergonic process. Performing CDR or Bioenergy with Carbon Capture and Storage (BECCS) at the gigatonne scale will produce significant additional burden on planet’s limited renewable energy resources irrespective of technology employed. Harnessing photosynthesis to drive industrial-scale fixation has been interest because its minimal requirements potential low costs. In this review, we evaluated thermodynamic considerations performing atmospheric carbon using microalgae cyanobacteria versus physicochemical processes explore implications these energetic costs scalability each respective solution. We review biomass productivities recently discovered fast-growing cyanobacterial strains discuss prospects genetically engineering certain metabolic pathways for channeling fixed into ‘carbon sinks’ further enhance their capture while concurrently extracting value. share our perspectives how new highly productive chassis combined advanced flux balance models, essentially coupling synthetic biology industrial biotechnology, may unlock more favorable methods CDR, both from an economic perspective.

Language: Английский

Citations

Peptide nucleic acids: Recent advancements and future opportunities in biomedical applications DOI

Achinta Sannigrahi, Nayan De, Debmalya Bhunia

et al.

Bioorganic Chemistry, Journal Year: 2025, Volume and Issue: 155, P. 108146 - 108146

Published: Jan. 10, 2025

Language: Английский

Citations

CRISPR/Cas technology in vegetable crops for improving biotic, abiotic stress and quality traits: Challenges and opportunities DOI

S. P. S. Kushwaha,

C. R. Nagesh,

S. S. Lele

et al.

Scientia Horticulturae, Journal Year: 2025, Volume and Issue: 341, P. 113957 - 113957

Published: Jan. 16, 2025

Language: Английский

Citations

Single nucleotide genome recognition and selective bacterial lysis using synthetic phages loaded with CRISPR-Cas12f1-truncated sgRNA DOI

Ho Joung Lee, Song Hee Jeong, Sang Jun Lee

et al.

The Journal of Microbiology, Journal Year: 2025, Volume and Issue: 63(2), P. e2501012 - e2501012

Published: Feb. 27, 2025

Language: Английский

Citations

From in vivo models to in vitro bioengineered neuromuscular junctions for the study of Charcot-Marie-Tooth disease DOI

Camille Scherrer,

Camille Loret, Nicolas Védrenne

et al.

Journal of Tissue Engineering, Journal Year: 2025, Volume and Issue: 16

Published: March 1, 2025

Peripheral neuropathies are disorders affecting the peripheral nervous system. Among them, Charcot-Marie-Tooth disease is an inherited sensorimotor neuropathy for which no effective treatment exists yet. Research on has been hampered by difficulties in accessing relevant cells, such as sensory and motor neurons, Schwann myocytes, interact at neuromuscular junction, specialized synapses formed between nerves skeletal muscles. This review first outlines various vivo models methods used to study junction deficiencies disease. We then explore novel vitro techniques models, including complex hiPSC-derived cultures, offer promising isogenic reproducible models. The adaptability of culture methods, cell origin, cell-type combinations, choice format, adds complexity excitement this rapidly evolving field. aims recapitulate available tools studying understand its pathophysiological mechanisms test potential therapies.

Language: Английский

Citations