A Comprehensive Review of Recent Research into the Effects of Antimicrobial Peptides on Biofilms—January 2020 to September 2023

Antibiotics, Journal Year: 2024, Volume and Issue: 13(4), P. 343 - 343

Published: April 9, 2024

Microbial biofilm formation creates a persistent and resistant environment in which microorganisms can survive, contributing to antibiotic resistance chronic inflammatory diseases. Increasingly, biofilms are caused by multi-drug microorganisms, which, coupled with diminishing supply of effective antibiotics, is driving the search for new therapies. In this respect, antimicrobial peptides (AMPs) short, hydrophobic, amphipathic that show activity against multidrug-resistant bacteria formation. They also possess broad-spectrum diverse mechanisms action. comprehensive review, 150 publications (from January 2020 September 2023) were collected categorized using terms ‘polypeptide agent’, ‘antimicrobial peptide’, ‘biofilm’. During period, wide range natural synthetic AMPs studied, LL-37, polymyxin B, GH12, Nisin most frequently cited. Furthermore, although many microbes Staphylococcus aureus Pseudomonas aeruginosa popular. Publications considered AMP combinations potential role delivery systems increasing efficacy AMPs, including nanoparticle delivery. Relatively few focused on resistance. This review informs guides researchers about latest developments research, presenting promising evidence as agents.

Language: Английский

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Advancing Nanotechnology: Targeting Biofilm-Forming Bacteria with Antimicrobial Peptides

BME Frontiers, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

Nanotechnology offers innovative solutions for addressing the challenges posed by biofilm-forming bacteria, which are highly resistant to conventional antimicrobial therapies. This review explores integration of pharmaceutical nanotechnology with peptides (AMPs) enhance treatment biofilm-related infections. The use various nanoparticle systems-including inorganic/metallic, polymeric, lipid-based, and dendrimer nanostructures-provides promising avenues improving drug delivery, targeting, biofilm disruption. These nanocarriers facilitate penetration biofilms, down-regulate biofilm-associated genes, such as ALS1, ALS3, EFG1, HWP1, inhibit bacterial defense mechanisms through membrane disruption, reactive oxygen species generation, intracellular targeting. Furthermore, formulations NZ2114-NPs demonstrate enhanced efficacy reducing counts several orders magnitude. highlights potential combining AMPs create novel, targeted therapeutic approaches combatting infections overcoming limitations traditional treatments.

Language: Английский

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Nanomedicines as disruptors or inhibitors of biofilms: Opportunities in addressing antimicrobial resistance

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: 381, P. 113589 - 113589

Published: March 1, 2025

The problem of antimicrobial resistance (AMR) has caused global concern due to its great threat human health. Evidences are emerging for a critical role biofilms, one the natural protective mechanisms developed by bacteria during growth, in resisting commonly used clinical antibiotics. Advances nanomedicines with tunable physicochemical properties and unique anti-biofilm provide opportunities solving AMR risks more effectively. In this review, we summarize five "A" stages (adhesion, amplification, alienation, aging allocation) biofilm formation through which they protect internal bacteria. Aimed at characteristics emphasize design "THAT" principles (targeting, hacking, adhering transport) their interactions biofilms Furthermore, recent progresses multimodal antibacterial nanomedicines, including disruption bactericidal activity, types currently available antibiofilm contained organic inorganic outlined highlighted potential applications development preclinical research. Last but not least, offer perspective effectiveness designed address challenges associated translation.

Language: Английский

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Silver and Antimicrobial Polymer Nanocomplexes to Enhance Biocidal Effects

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(2), P. 1256 - 1256

Published: Jan. 19, 2024

Antimicrobial resistance has become a major problem over the years and threatens to remain in future, at least until solution is found. Silver nanoparticles (Ag-NPs) antimicrobial polymers (APs) are known for their properties can be considered an alternative approach fighting resistant microorganisms. Hence, main goal of this research shed some light on Ag-NPs APs (chitosan (CH), poly-L-lysine (PLL), ε-poly-L-lysine (ε-PLL), dopamine (DA)) when used alone complexed explore potential enhancement effect combination + Aps. The resultant nanocomplexes were chemically morphologically characterized by UV-visible spectra, zeta potential, transmission electron microscopy, Fourier-transform infrared spectroscopy. Moreover, Ag-NPs, APs, tested against Gram-positive Staphylococcus aureus (S. aureus) Gram-negative Escherichia coli (E. coli) bacteria, as well fungi Candida albicans (C. albicans). Overall, results showed potentiation activity with focus C. albicans. For biofilm eradication ability, DA able significantly remove S. preformed biofilm, CH destroy both performing better than alone. we have proven successful conjugation these formulations showing further investigated treatment microbial infections.

Language: Английский

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Antimicrobial activity of metal-based nanoparticles: a mini-review

BioMetals, Journal Year: 2024, Volume and Issue: 37(4), P. 773 - 801

Published: Jan. 29, 2024

Language: Английский

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Functional analysis and modification of anti-lipopolysaccharide factor (ALF) from the freshwater crab Sinopotamon henanense and preparation of a novel ShALF6-2 K-AgNPs complex

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 302, P. 139874 - 139874

Published: Jan. 22, 2025

Language: Английский

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Enhancing the antibacterial effect of iron oxide and silver nanoparticles by extremely low frequency electric fields (ELF-EF) against S. aureus

Electromagnetic Biology and Medicine, Journal Year: 2023, Volume and Issue: 42(3), P. 99 - 113

Published: May 8, 2023

Staphylococcus aureus is the cause of many infectious and inflammatory diseases a lot studies aim to discover alternative ways for infection control treatment rather than antibiotics. This work attempts reduce bacterial activity growth characteristics using nanoparticles (iron oxide silver nanoparticles) extremely low frequency electric fields (ELF-EF). Bacterial suspensions were used prepare samples, which evenly divided into groups. Control group, 10 groups exposed ELF-EF in range (0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1 Hz), iron NPs treated 0.8 Hz group last was with Hz. Antibiotic sensitivity testing, dielectric relaxation, biofilm development living microbe evaluate morphological molecular alterations. Results showed that combination at enhanced inhibition efficiency, may be due structural changes. These supported by measurement results indicated differences increment electrical conductivity samples compared samples. also confirmed formation measurements obtained. We conclude exposure bacteria affected its cellular structure. technique nondestructive, safe fast could considered as mean use

Language: Английский

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Biofilm modifiers: The disparity in paradigm of oral biofilm ecosystem

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 164, P. 114966 - 114966

Published: June 1, 2023

A biofilm is a population of sessile microorganisms that has distinct organized structure and characteristics like channels projections. Good oral hygiene reduction in the prevalence periodontal diseases arise from minimal accumulation mouth, however, studies focusing on modifying ecology biofilms have not yet been consistently effective. The self-produced matrix extracellular polymeric substances greater antibiotic resistance make it difficult to target eliminate infections, which lead serious clinical consequences are often lethal. Therefore, better understanding required modify order eradicate infection, only instances disorders but also terms nosocomial infections. review focuses several modifiers prevent as well involvement resistance, implants or in-dwelling device contamination, dental caries, other disorders. It discusses recent advances nanotechnology may novel strategies for preventing treating infections caused by outlook infection control.

Language: Английский

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Antimicrobial surface coating as a pathway to curb resistance: preparation, mode of action and future perspective

Journal of Coatings Technology and Research, Journal Year: 2024, Volume and Issue: 21(3), P. 799 - 810

Published: Feb. 12, 2024

Language: Английский

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Ulvan/Silver nanoparticle hydrogel films for burn wound dressing

Heliyon, Journal Year: 2023, Volume and Issue: 9(7), P. e18044 - e18044

Published: July 1, 2023

Ulvan is a polysaccharide from green algae that shows good hydrogel film dressing characteristics. Silver nanoparticles (AgNP) can be incorporated into the to improve antibacterial properties and provide potential burn treatment. In this study, we developed novel wound composed of ulvan silver nanoparticles. Two concentrations (0.5 mM 1 mM) nitrate were used produce ulvan-silver (UHF-AgNP0.5 UHF-AgNP1), respectively. The physicochemical characteristics films evaluated, including particle size, zeta potential, Fourier transform infrared (FTIR), X-ray diffractometry (XRD), scanning electron microscope energy-dispersive (SEM-EDX). Furthermore, in vitro antimicrobial activity, second-degree healing test evaluated. UHF-AgNP0.5 showed highest activity compared UHF-AgNP1 UHF film. Meanwhile, an vivo study using Wistar rats induced burns significantly accelerated process by regulating inflammatory process, increasing re-epithelialization, improving vascularization process. Ulvan-silver nanoparticle have ability accelerate are candidates for dressings.

Language: Английский

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