Zinc Oxide Nanoparticles as Potential Delivery Carrier: Green Synthesis by Aspergillus niger Endophytic Fungus, Characterization, and In Vitro/In Vivo Antibacterial Activity

Pharmaceuticals, Journal Year: 2022, Volume and Issue: 15(9), P. 1057 - 1057

Published: Aug. 26, 2022

We aimed to synthesize zinc oxide nanoparticles (ZnO NPs) using the endophytic fungal extract of Aspergillus niger. The prepared ZnO NPs were characterized, and their in vitro vivo antibacterial activity was investigated. Isolated fungus identification carried out 18S rRNA. A. niger employed for green synthesis NPs. elucidated against Staphylococcus aureus broth microdilution method quantitative real-time polymerase chain reaction (qRT-PCR). Additionally, a systemic infection model mice. biosynthesized showed maximum optical density at 380 nm with characteristic peaks on Fourier-transform infrared spectrum. X-ray diffraction pattern highly matched standard platform crystals. Energy-dispersive analysis confirmed that main composition oxygen atoms. Scanning transmission electron microscopies spherical geometry smooth surface. Zeta potential measurements (26.6 ± 0.56 mV) verified adequate stability Minimum inhibitory concentrations S. isolates ranged from 8 128 µg/mL. revealed antibiofilm activity, resulting downregulation tested biofilm genes 29.17% isolates. Regarding experiment, reduced congestion fibrosis liver spleen tissues. They also improved function, increased survival rate, significantly decreased inflammatory markers (p < 0.05). synthesized by promising action

Language: Английский

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Anti-Biofilm and Antibacterial Activities of Cycas media R. Br Secondary Metabolites: In Silico, In Vitro, and In Vivo Approaches

Antibiotics, Journal Year: 2022, Volume and Issue: 11(8), P. 993 - 993

Published: July 24, 2022

Enterococcus species possess many virulence factors that have an essential role in exacerbating the infections caused by them. The current study aimed to evaluate effect of secondary metabolites ginkgetin (GINK) and sotetsuflavone (SOTE), isolated from Cycas media R. Br dichloromethane fraction, on faecalis (E. faecalis) isolates for first time. antibacterial antivirulence activities compounds were investigated using docking studies vitro determination minimum inhibitory concentrations (MICs). Additionally, flow cytometry scanning electron microscope (SEM) utilized assess SOTE tested bacteria. Moreover, crystal violet assay qRT-PCR used test biofilm-forming ability E. isolates. In addition, a systemic infection model was vivo investigate activity SOTE. We found both GINK showed good affinity five proteins enrolled faecalis, with being highest, suggesting possible mechanisms ligands. exhibited higher than GINK, as values MICs lower those GINK. Thus, we performed assays However, they did not exhibit any significant variations (p > 0.05) membrane depolarization evaluated SEM, distortion deformation treated cells. Regarding its impact biofilm formation, it inhibited isolates, determined qRT-PCR. experiment revealed resulted reduction inflammation liver spleen increase survival rate. also improved liver-function tests decreased tumor necrosis factor-alpha immunostaining markers, interleukins (IL-1β IL-6), ELISA. can conclude could be promising compound should future preclinical clinical studies.

Language: Английский

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PLGA-Based Micro/Nanoparticles: An Overview of Their Applications in Respiratory Diseases

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(5), P. 4333 - 4333

Published: Feb. 22, 2023

Respiratory diseases, such as asthma and chronic obstructive pulmonary disease (COPD), are critical areas of medical research, millions people affected worldwide. In fact, more than 9 million deaths worldwide were associated with respiratory diseases in 2016, equivalent to 15% global deaths, the prevalence is increasing every year population ages. Due inadequate treatment options, treatments for many limited relieving symptoms rather curing disease. Therefore, new therapeutic strategies urgently needed. Poly (lactic-co-glycolic acid) micro/nanoparticles (PLGA M/NPs) have good biocompatibility, biodegradability unique physical chemical properties, making them one most popular effective drug delivery polymers. this review, we summarized synthesis modification methods PLGA M/NPs their applications (asthma, COPD, cystic fibrosis (CF), etc.) also discussed research progress current status diseases. It was concluded that promising vehicles due advantages low toxicity, high bioavailability, loading capacity, plasticity modifiability. And at end, presented an outlook on future directions, aiming provide some ideas directions hopefully promote widespread application clinical treatment.

Language: Английский

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Co-delivery of gentiopicroside and thymoquinone using electrospun m-PEG/PVP nanofibers: In-vitro and In vivo studies for antibacterial wound dressing in diabetic rats

International Journal of Pharmaceutics, Journal Year: 2022, Volume and Issue: 625, P. 122106 - 122106

Published: Aug. 24, 2022

Language: Английский

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The wound healing effect of botanicals and pure natural substances used in in vivo models

Inflammopharmacology, Journal Year: 2023, Volume and Issue: 31(2), P. 755 - 772

Published: Feb. 22, 2023

Repairing the wound is a multistep process that includes spatial and temporal synchronization of different range cell types to increase speed contraction, proliferation epithelial cells, collagen formation. The need for proper management acute wounds be cured not turned into chronic significant clinical challenge. traditional practice medicinal plants in many regions world has been used healing since ancient times. Recent scientific research introduced evidence efficacy plants, their phyto-components, mechanisms underlying wound-repairing activity. This review aims briefly highlight wound-curing effect plant extracts purely natural substances excision, incision, burn experimental animal models with or without infection mice, rats (diabetic nondiabetic), rabbits last 5 years. vivo studies represented reliable how powerful products are properly. They have good scavenging activity against Reactive oxygen species (ROS) anti-inflammatory antimicrobial effects help healing. It evident incorporating bioactive dressings bio- synthetic polymers nanofiber, hydrogel, film, scaffold, sponge forms showed promising results phases haemostasis, inflammation, growth, re-epithelialization, remodelling.

Language: Английский

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Preparation of stable Fe₂O₃/Ag nanocomposite particles with catalytic, antioxidant and antibacterial properties

New Journal of Chemistry, Journal Year: 2024, Volume and Issue: 48(12), P. 5278 - 5288

Published: Jan. 1, 2024

Schematic diagram showing the preparation process of stable Fe 2 O 3 /Ag nanocomposite particles.

Language: Английский

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Aqueous core epigallocatechin gallate PLGA nanocapsules: characterization, antibacterial activity against uropathogens, andin vivoreno-protective effect in cisplatin induced nephrotoxicity

Drug Delivery, Journal Year: 2022, Volume and Issue: 29(1), P. 1848 - 1862

Published: June 16, 2022

Epigallocatechin-3-gallate (EGCG) was isolated from Cycas thouarsii leaves for the first time and encapsulated in aqueous core poly(lactide-co-glycolide) (PLGA) nanocapsules (NCs). This work investigates antimicrobial activity vivo reno-protective effects of EGCG-PLGA NCs cisplatin-induced nephrotoxicity. A double emulsion solvent evaporation process adopted to prepare PLGA loaded with EGCG. Particle size, polydispersity index (PDI), zeta potential, percent entrapment efficiency (%EE), structural morphology, vitro release platform were all studied vitro. The optimum formula (F2) particle size (61.37 ± 5.90 nm), PDI (0.125 0.027), potential (-11.83 3.22 mV), %EE (85.79 5.89%w/w), initial burst (36.85 4.79), cumulative (87.79 9.84) selected further vitro/in studies. F2 exhibited an enhanced against uropathogens as it had lower minimum inhibitory concentration (MIC) values a more significant impact on bacterial growth than free Forty male adult mice randomly allocated into five groups: control vehicle, untreated methotrexate, MTX groups treated daily oral dose EGCG, placebo NCs, EGCG 10 days. Results showed that exerted promising renoprotective compared group induced decrease kidney index, serum creatinine, injury molecule-1 (KIM-1), NGAL levels, pronounced inhibition NLPR-3/caspase-1/IL/1β inflammasome pathway. It also significantly ameliorated oxidative stress decreased NFκB, Bax expression levels. Aqueous are formulation strategy provides high polymeric protection sustained pattern hydrophilic therapeutic agents.

Language: Английский

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Iridoids modulate inflammation in diabetic kidney disease: A review

Journal of Integrative Medicine, Journal Year: 2024, Volume and Issue: 22(3), P. 210 - 222

Published: March 28, 2024

Language: Английский

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Encapsulation of natural drug gentiopicroside into zinc based Zeolitic Imidazolate Frameworks (ZIF-8): In-vitro drug release and improved antibacterial activity

Journal of Drug Delivery Science and Technology, Journal Year: 2023, Volume and Issue: 84, P. 104530 - 104530

Published: May 5, 2023

Language: Английский

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Formulation and characterization of quercetin-loaded Prunus armeniaca gum nanoparticles with enhanced anti-bacterial effect

Journal of Drug Delivery Science and Technology, Journal Year: 2024, Volume and Issue: 94, P. 105485 - 105485

Published: Feb. 17, 2024

Language: Английский

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