Metabolic Profiling of Brain Tissue and Brain‐Derived Extracellular Vesicles in Alzheimer's Disease

Journal of Extracellular Vesicles, Journal Year: 2025, Volume and Issue: 14(2)

Published: Feb. 1, 2025

ABSTRACT Alzheimer´s disease (AD) is the most frequent neurodegenerative disorder in world and characterised by loss of memory other cognitive functions. Metabolic changes associated with AD are important players development disease. However, mechanism underlying these still unknown. Extracellular vesicles (EVs) nano‐sized particles that play an role regulating pathophysiological processes a non‐invasive manner to obtain information cell secreting them. The analysis brain‐derived EVs (bdEVs) will provide new insights metabolic AD. To characterize bdEVs AD, we optimised method isolate them from tissue different brain regions, obtaining highest enrichment isolations temporal cortex. We performed unbiased untargeted metabolomics on post‐mortem human cortex same region patients healthy controls. Both, univariate multivariate statistical were used determine metabolites influence separation between Interestingly, clear control groups was obtained bdEVs, which allowed select 12 relevant features validated PLS‐DA model. Furthermore, comparison identified 68 common features. pathway showed alanine, aspartate glutamate arginine, phenylalanine, tyrosine significant ones tryptophan pathway, had very high groups, albeit not significant. Notably, some for first time bdEVs. For example, N‐acetyl aspartic acid (NAA) metabolite present suitable differentiate hippocampus, midbrain, entorhinal their respective indicated profiles areas distinct correlation metabolome its Thus, our study highlights potential understand fingerprint use as diagnostic therapeutic targets.

Language: Английский

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Overview of the characterization of extracellular vesicles

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 25 - 56

Published: Jan. 1, 2025

Language: Английский

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Unveiling exosomal biomarkers in neurodegenerative diseases: LC-MS-based profiling

Extracellular Vesicle, Journal Year: 2025, Volume and Issue: 5, P. 100071 - 100071

Published: March 22, 2025

Language: Английский

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Bibliometric analysis of exosomes in the biomarker research field

Chinese Journal of Chromatography, Journal Year: 2025, Volume and Issue: 43(5), P. 498 - 507

Published: May 1, 2025

Exosomes are extracellular vesicles secreted by cells and rich in genetic material proteins. The surfaces of exosome membranes contain many blast-specific markers that provide an important basis for disease diagnosis, progression, treatment. Herein, we searched the Web Science core collection (SCI-EXPENED) database research review articles on "exosomes" "biomarkers" or "diagnostics" "liquid biopsy" as topics between 2010 2024. Bibliometric analysis revealed exosomes have received increasing levels attention biomarkers, with China contributing most to these studies. focus marker diagnoses cancer, inflammation, diabetes, well neurodegenerative cardiovascular diseases. Chromatography, mass spectrometry, Raman spectroscopy, other techniques typically used analyze exosomal nucleic acids, proteins, metabolites, commonly test samples including plasma, serum, urine, saliva, cerebrospinal fluid, bodily fluids. Research into tumor has mainly focused eight highly prevalent cancers, lung, breast, prostate cancers. This paper focuses biomarkers uses a bibliometric tool system use their contents diagnosis field 2024, analyzes development prospects, discusses future exosome-mediated efforts diagnosing treating diseases, is expected reference studying applying markers.

Language: Английский

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Metabolic profiling in tissues and urine of patients with prostatic lesions and the diagnostic value of urine extracellular vesicles metabolites in prostate cancer

Clinica Chimica Acta, Journal Year: 2024, Volume and Issue: 556, P. 117845 - 117845

Published: Feb. 23, 2024

Language: Английский

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Preparation of Dysprosium(III)-Metal Organic Framework Nanofiber for Exosome Capture and Biomarker Discovery toward Liver Disease

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(42), P. 56874 - 56883

Published: Oct. 11, 2024

As an emerging source for liquid biopsy, exosomes hold significant promise clinical diagnosis. However, commonly used exosome isolation methods (e.g., ultracentrifugation) suffer from low throughput a large number of samples. Herein, dysprosium–metal organic framework was synthesized and doped with nanofibers by electrospinning efficient capture body fluid. With the integration multichannel pipet or robot automatic workstation, high can be achieved samples reproducibility. To evaluate value developed method, urinary were enriched 34 liver disease different stages profiling metabolites mass spectrometry. The results showed that HCC, cirrhosis, healthy controls significantly differentiated Random Forest classification model. has promising applications in exosome-based biopsy large-scale

Language: Английский

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Concise review: Breastfeeding, lactation, and NAFLD. An updated view of cross-generational disease transmission and prevention

Metabolism and Target Organ Damage, Journal Year: 2023, Volume and Issue: 3(4)

Published: Sept. 21, 2023

Evidence suggests that breastfeeding protects the mother-infant dyad against development and progression of nonalcoholic fatty liver disease (NAFLD). In this context, we aim to provide insight into most notable representative epidemiological studies published in literature. Furthermore, will delve potential underlying pathomechanisms might be involved relationship. The current definitions breastfeeding, lactation, dyad, (NAFLD) are provided. Next, evidence supporting benefits for (long-term) lactating mother terms protection from NAFLD is reviewed. putative mechanisms also analyzed. Similarly, clinical evaluating offspring examined, together with a discussion mechanisms. conclusion, our understanding (for offspring) lactation mother) as protective factors fibrotic further unprecedented shared by promising interrupt vicious cycle transmission across generations.

Language: Английский

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Thermophoretic Aggregation Imaging of Tumor-Derived Exosomes by CRISPR-Cas13a-Based Nanoprobes

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(12), P. 4791 - 4799

Published: March 12, 2024

The inherent heterogeneity of tumor-derived exosomes holds great promise for enhancing the precision cancer diagnostics. MicroRNAs (miRNAs) encapsulated in tumor-associated have emerged as valuable biomarkers early detection cancers. Nevertheless, flexible structure and instability RNA limit its application biological CRISPR-Cas13a system, distinguished by target-responsive "collateral effect", represents a powerful tool advancing In this study, we harness system an innovative signal amplification to image cancer-related exosomal miRNA situ. Furthermore, capitalize on thermophoretic aggregation effect exhibited gold nanoparticles (Au NPs) consolidate fluorescent signals generated system. Subsequently, developed nanoprobe is applied detect lung from human serum, enabling aggregated visualization low-abundance individuals with compared healthy individuals. This sensitive assay provides diagnostic clinic.

Language: Английский

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Zika Virus in Extracellular Vesicles: Insights from Integrated Proteomic and Metabolomic Dependent Regulation of B Cell and PI3K/AKT/mTOR Signaling Pathway

Published: Aug. 12, 2024

Background; Cell-released extracellular vesicles (EVs) acting as 'metabolically and proteolytically active machines,' show potential in metabolomic proteomic analysis of serum EVs. Despite diverse challenges, post-isolation omics characterization EVs offers crucial insights for effective analysis; (2) Methods: The research, involved children with Congenital Zika Syndrome, utilizing mass spectrometry proteomics GC-MS metabolite identification. Vesicles were isolated using Izon qEV columns, quantified, characterized by NTA TEM. Data employed Cytoescape/String MetaboAnalyst, revealing variations metabolic profiles among groups through PCA volcano plots. Proteins Metabolite set enrichment provided biologically meaningful patterns to enriched metabolites; (3) Results: Using molecular exclusion chromatography, the characterized, size variations. Protein identified 13 significantly altered proteins, including upregulated (e.g., AOM8Q6 - IGLC7) downregulated Q8TD86 CALML6) ones. indicated involvement PI3K-AKT-mTOR pathway suggested a role Angiotensin inhibition CZS+. Upstream mTOR, Akt is central signaling molecule PI3K plays critical roles brain development well synaptic plasticity important Virus. study provides into mechanisms associated CZS; (4) Conclusions: pinpointed valuable possible biomarkers, specifically proteins metabolites, virus (ZIKV) infection. It stresses necessity broader investigations advanced techniques uncover targets, potentially advancing pharmacological strategies.

Language: Английский

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Surface-enhanced infrared spectroscopic study of extracellular vesicles using plasmonic gold nanoparticles

Colloids and Surfaces B Biointerfaces, Journal Year: 2024, Volume and Issue: 246, P. 114366 - 114366

Published: Nov. 7, 2024

Language: Английский

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