Nitric Oxide-Releasing Mesoporous Hollow Cerium Oxide Nanozyme-Based Hydrogel Synergizes with Neural Stem Cell for Spinal Cord Injury Repair DOI
Dun Liu,

Runyan Niu,

Siliang Wang

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 19(2), P. 2591 - 2614

Published: Dec. 26, 2024

Neural stem cell (NSCs) transplantation is a promising therapeutic strategy for spinal cord injury (SCI), but its efficacy greatly limited by the local inhibitory microenvironment. In this study, based on l-arginine (l-Arg)-loaded mesoporous hollow cerium oxide (AhCeO2) nanospheres, we constructed an injectable composite hydrogel (AhCeO2-Gel) with microenvironment modulation capability. AhCeO2-Gel protected NSCs from oxidative damage eliminating excess reactive oxygen species while continuously delivering Nitric Oxide to lesion of SCI in pathological microenvironment, latter which effectively promoted neural differentiation NSCs. The process was confirmed be closely related up-regulation cAMP-PKA pathway after NO-induced calcium ion influx. addition, significantly polarization microglia toward M2 subtype as well enhanced regeneration nerves and myelinated axons. prepared bioactive system also efficiently facilitated integration transplanted host circuits, replenished damaged neurons, alleviated neuroinflammation, inhibited glial scar formation, thus accelerating recovery motor function rats. Therefore, synergized has great potential integrated treat comprehensively reversing

Language: Английский

Harnessing the potential of hydrogels for advanced therapeutic applications: current achievements and future directions DOI Creative Commons

Peilin Lu,

Dongxue Ruan,

Meiqi Huang

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2024, Volume and Issue: 9(1)

Published: July 1, 2024

The applications of hydrogels have expanded significantly due to their versatile, highly tunable properties and breakthroughs in biomaterial technologies. In this review, we cover the major achievements potential therapeutic applications, focusing primarily on two areas: emerging cell-based therapies promising non-cell modalities. Within context cell therapy, discuss capacity overcome existing translational challenges faced by mainstream therapy paradigms, provide a detailed discussion advantages principal design considerations for boosting efficacy as well list specific examples different disease scenarios. We then explore drug delivery, physical intervention therapies, other areas (e.g., bioadhesives, artificial tissues, biosensors), emphasizing utility beyond mere delivery vehicles. Additionally, complement our latest progress clinical application outline future research directions, particularly terms integration with advanced biomanufacturing This review aims present comprehensive view critical insights into selection both tailored meet requirements diverse diseases situations.

Language: Английский

Citations

83
Hydrogel loaded with cerium-manganese nanoparticles and nerve growth factor enhances spinal cord injury repair by modulating immune microenvironment and promoting neuronal regeneration DOI Creative Commons
Zhaoyang Gong, Zhenhao Chen, Dachuan Li

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 20, 2025

Spinal cord injury (SCI) treatment remains a formidable challenge, as current therapeutic approaches provide only marginal relief and fail to reverse the underlying tissue damage. This study aims develop novel composite material combining enzymatic nanoparticles nerve growth factor (NGF) modulate immune microenvironment enhance SCI repair. CeMn (NP) NP-polyethylene glycol (PEG) nanozymes were synthesized via sol–gel reaction DSPE-mPEG modification. Transmission Electron Microscopy, Selected-Area Diffraction, X-ray Diffraction Photoelectron Spectroscopy confirmed their crystalline structure, mixed-valence states, redox properties. Size uniformity, biocompatibility, catalytic activity assessed hydrodynamic diameter, zeta potential, elemental analysis. The Lightgel/NGF/CeMn NP-PEG was characterized electron microscopy, compression testing, rheological analysis, NGF release kinetics, 30-day degradation studies. Both in vitro vivo experiments conducted evaluate effects of on SCI. successfully synthesized, exhibiting favorable physical At concentration 4 µg/mL, maintained cell viability demonstrated enhanced biological activity. It also showed superior mechanical properties an effective profile. Notably, significantly upregulated expression growth-associated proteins, reduced inflammatory cytokines, scavenged reactive oxygen species (ROS), promoted M2 macrophage polarization by inhibiting cyclic GMP-AMP synthase (cGAS)-stimulator interferon genes (STING) signaling pathway. In rat model, it facilitated functional recovery attenuated inflammation. shows significant promise for SCI, effectively eliminating ROS, promoting polarization, reducing pro-inflammatory supporting neuronal regeneration. These substantially motor function rats, positioning promising candidate future clinical applications.

Language: Английский

Citations

2
Constructing Nerve Guidance Conduit using dECM‐Doped Conductive Hydrogel to Promote Peripheral Nerve Regeneration DOI
Lizhao Yan, Shuang Liu, Jianwen Wang

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(38)

Published: April 12, 2024

Abstract Peripheral nerve injury often leads to the loss of neurological functions due slow regeneration rate and inefficient functional reconstruction. Current clinical treatments using guidance conduits (NGCs) still face challenges in providing a biomimetic microenvironment promote repair. Herein, decellularized extracellular matrix (dECM) is obtained from porcine Achilles tendon crosslinked with 3‐amino‐4‐methoxybenzoic acid grafted gelatin (PAMB‐G) obtain conductive hydrogels. Then, novel conduit developed by assembling poly(vinyl alcohol) (PVA) ECM@PAMB‐G hydrogel. This bioengineered ECM@PAMB‐G/PVA demonstrated excellent cytocompatibility, electrical conductivity, mechanical properties, biodegradability. In vitro experiments confirmed that hydrogel significantly promotes proliferation migration PC12 cells primary Schwann cells, as well growth dorsal root ganglion (DRG) axons. Furthermore, vivo studies rat sciatic model exhibited improvements axonal regeneration, cell migration, myelin sheath formation, recovery mediated conduit. work demonstrates synergistic effects cues enhancing peripheral regeneration. The shows potential an alternative autografts for supporting

Language: Английский

Citations

10
Functional biomaterials for modulating the dysfunctional pathological microenvironment of spinal cord injury DOI Creative Commons

Dezun Ma,

Changlong Fu,

Fenglu Li

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 39, P. 521 - 543

Published: May 30, 2024

Spinal cord injury (SCI) often results in irreversible loss of sensory and motor functions, most SCIs are incurable with current medical practice. One the hardest challenges treating SCI is development a dysfunctional pathological microenvironment, which mainly comprises excessive inflammation, deposition inhibitory molecules, neurotrophic factor deprivation, glial scar formation, imbalance vascular function. To overcome this challenge, implantation functional biomaterials at site has been regarded as potential treatment for modulating microenvironment to support axon regeneration, remyelination site, recovery after SCI. This review summarizes characteristics recent advances well technologies used modulate inflammatory regulate reshape revascularization microenvironment. Moreover, technological limitations, challenges, future prospects promote efficient repair also discussed. will aid further understanding

Language: Английский

Citations

10
Hydrogels for Peripheral Nerve Repair: Emerging Materials and Therapeutic Applications DOI Creative Commons

Oana Taisescu,

Venera Cristina Dinescu, Alexandra Daniela Rotaru-Zavaleanu

et al.

Gels, Journal Year: 2025, Volume and Issue: 11(2), P. 126 - 126

Published: Feb. 9, 2025

Peripheral nerve injuries pose a significant clinical challenge due to the complex biological processes involved in repair and their limited regenerative capacity. Despite advances surgical techniques, conventional treatments, such as autografts, are faced with limitations like donor site morbidity inconsistent functional outcomes. As such, there is growing interest new, novel, innovative strategies enhance regeneration. Tissue engineering/regenerative medicine its use of biomaterials an emerging example strategy. Within realm tissue engineering, functionalized hydrogels have gained considerable attention ability mimic extracellular matrix, support cell growth differentiation, even deliver bioactive molecules that can promote repair. These be engineered incorporate factors, peptides, stem cells, creating conducive microenvironment for cellular axonal Recent advancements materials well biology led development sophisticated hydrogel systems, not only provide structural support, but also actively modulate inflammation, recruitment, stimulate neurogenesis. This review explores potential peripheral repair, highlighting composition, biofunctionalization, mechanisms action. A comprehensive analysis preclinical studies provides insights into efficacy these promoting growth, neuronal survival, regeneration, and, ultimately, recovery. Thus, this aims illuminate promise transformative tool field bridging gap between complexity feasibility.

Language: Английский

Citations

1
Electrically Conductive Coatings in Tissue Engineering DOI
Abolfazl Anvari Kohestani, Zhiyan Xu, Fatih Erdem Baştan

et al.

Acta Biomaterialia, Journal Year: 2024, Volume and Issue: 186, P. 30 - 62

Published: Aug. 14, 2024

Language: Английский

Citations

8
Advances in electroactive bioscaffolds for repairing spinal cord injury DOI
Zeqi Liu,

Jiahui Lai,

Dexin Kong

et al.

Biomedical Materials, Journal Year: 2024, Volume and Issue: 19(3), P. 032005 - 032005

Published: April 18, 2024

Spinal cord injury (SCI) is a devastating neurological disorder, leading to loss of motor or somatosensory function, which the most challenging worldwide medical problem. Re-establishment intact neural circuits basis spinal regeneration. Considering crucial role electrical signals in nervous system, electroactive bioscaffolds have been widely developed for SCI repair. They can produce conductive pathways and pro-regenerative microenvironment at lesion site similar that natural cord, neuronal regeneration axonal growth, functionally reactivating damaged circuits. In this review, we first demonstrate pathophysiological characteristics induced by SCI. Then, repair introduced. Based on comprehensive analysis these characteristics, recent advances are summarized, focusing both piezoelectric bioscaffolds, used independently combination with external electronic stimulation. Finally, thoughts challenges opportunities may shape future concluded.

Language: Английский

Citations

5
Innovative Strategies in 3D Bioprinting for Spinal Cord Injury Repair DOI Open Access

Daniel Youngsuk Kim,

Yanting Liu,

Gyubin Kim

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9592 - 9592

Published: Sept. 4, 2024

Spinal cord injury (SCI) is a catastrophic condition that disrupts neurons within the spinal cord, leading to severe motor and sensory deficits. While current treatments can alleviate pain, they do not promote neural regeneration or functional recovery. Three-dimensional (3D) bioprinting offers promising solutions for SCI repair by enabling creation of complex tissue constructs. This review provides comprehensive overview 3D techniques, bioinks, stem cell applications in repair. Additionally, it highlights recent advancements bioprinted scaffolds, including integration conductive materials, incorporation bioactive molecules like neurotrophic factors, drugs, exosomes, design innovative structures such as multi-channel axial scaffolds. These strategies offer approach optimizing microenvironment, advancing understanding state repair, offering insights into future directions field regenerative medicine.

Language: Английский

Citations

4
Multifunctional magneto-electric and exosome-loaded hydrogel enhances neuronal differentiation and immunoregulation through remote non-invasive electrical stimulation for neurological recovery after spinal cord injury DOI
Wubo Liu, Qiang Liu, Zeqin Li

et al.

Bioactive Materials, Journal Year: 2025, Volume and Issue: 48, P. 510 - 528

Published: Feb. 28, 2025

Language: Английский

Citations

0
DNA Hydrogels in Tissue Engineering: From Molecular Design to Next‐Generation Biomedical Applications DOI
Ziyu Zhu, Yu Yang, Yun Jiang

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

Abstract DNA hydrogels have emerged as promising materials in tissue engineering due to their biocompatibility, programmability, and responsiveness stimuli. Synthesized through physical chemical crosslinking, these can be categorized into functionalized types, such those based on aptamers, stimuli‐responsive types that react pH, temperature, light. This review highlights applications engineering, including drug delivery, cell culture, biosensing, gene editing. encapsulate therapeutic agents, support growth, respond dynamically environmental changes, making them ideal for engineering. A comprehensive bibliometric analysis is included, identifying key research trends emerging areas of interest hydrogel design, synthesis, biomedical applications. The provides a deeper understanding the field's development future directions. Challenges mechanical strength, stability, biosafety persist, but integration AI design shows promise advancing functionality clinical

Language: Английский

Citations

0