Shell-core-structured electrospinning film with sequential anti-inflammatory and pro-neurogenic effects for peripheral nerve repairment

Nanotechnology Reviews, Journal Year: 2024, Volume and Issue: 13(1)

Published: Jan. 1, 2024

Abstract Inducing an anti-inflammatory response before neurogenesis is crucial in effectively addressing peripheral nerve damage. Herein, we developed shell-core-structured nanofilms (Cur/PLCL@BDNF/CNT) using poly-( l -Lactide)-Caprolactone (PLCL) and curcumin (Cur, agent) as the shell layer, carbon nanotubes (CNT) brain-derived neurotrophic factor (BDNF, a neurogenic factor) core via coaxial electrospinning technology. The resulting Cur/PLCL@BDNF/CNT film exhibited characteristic fibrous structure with remarkable shell-core architecture, demonstrating unweakened mechanical properties. Notably, it displayed sustained release kinetics distinct stages: prioritized Cur within initial 12 days delayed BDNF between 10 to 30 days. Additionally, demonstrated high biocompatibility Schwann cells. Subsequent vitro analysis revealed potent capabilities of released from while layer induced differentiation was rolled into conduit then utilized for regeneration mm rat sciatic defect model. staged facilitated by established microenvironment initiating regeneration, better restoration. This study emphasizes significance temporally regulating anti-inflammation neurogenesis.

Language: Английский

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Response to Article “Development of a Magnetically-Assisted SERS Biosensor for Rapid Bacterial Detection” [Letter]

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 5951 - 5952

Published: June 1, 2024

Infections by bacteria cause significant health issues, affecting approximately 258 million people globally and leading to 280,000 deaths every year. 1 In particular, Staphylococcus aureus Pseudomonas aeruginosa are the dangerous, often causing pneumonia, bone heart infections. 2 At present, polymerase chain reaction (PCR) has been widely used as a reliable excellent diagnostic technique at genetic level confirm identify infected pathogen. 3However, PCR is expensive, time-consuming some specific primers different microorganisms, physicians need list potential microorganisms before performing selective PCR.Therefore, providing accurate rapid identification for become mandatory wide applications.In recent research, Cheng his group developed sandwich SARS immunoassay using magnetic nanoparticles (MGNP), wheat germ agglutinin (WGA), gold nanostars (AuNS) identifying bacterial pathogens. 4As indicated in this nanomaterials commonly enhance Raman signal diagnosing pathogenic bacteria. 5heng et al antibodies S. P. aeruginosa, conjugated with MGNP, capture probe.AuNS was modified WGA 5.5'-dithiobis-(2-nitrobenzoic acid) utilized signalling complex (SERS tag).Target were captured antibody-conjugated MGNPs combined SERS tags form complex.Since performance of sensor highly relies on influences tag, displayed highest binding affinity aeruginosa.This newly introduced AuNS, proved have strong capabilities, evidenced tag attachments surface intact bacteria, increasing signal.Additionally, intensity enhanced proportionally concentrations, limits detection improved 5 7 CFU/mL, respectively.With spiked samples urine, researchers higher concentrations (50, 500, 5000 CFU/mL), clear observed.However, it would be better use lower match levels urine samples.Overall, current research shows pathogen nanomaterial platform that can mimicked other novel nanomaterials.The novelty work promising, biosensor model could well biomarkers diagnose diseases earlier stages.Furthermore, similar sensing might created open up new possibilities high-performance point-of-care testing.

Language: Английский

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Evaluating Hepatic Toxicity of Chitosan and Chitosan- Functionalized Iron Oxide Nanoparticles in a Rat Model of Peripheral Nerve Injury — Relevance for Rehabilitation Medicine

Balneo and PRM Research Journal, Journal Year: 2024, Volume and Issue: 15(Vol.15, no. 2), P. 680 - 680

Published: June 21, 2024

Background: Peripheral nerve injuries (PNI) can generate important medium- and long-term disability, as patients mostly complain about associated pain, sensibility and, or motor deficit, even psychological manifestations. Chitosan different nanoparticles types were previously used in several studies treatment of peripheral injuries. The present study aimed to assess the hepatic responses at oral administration simple chitosan solution magnetic iron oxide functionalized with (CMNPs) an experimentally induced injury. (2) Methods: CMNPs administrated, for 21 days, animals treated groups compared a control group (peripheric injury without any treatment). toxicity administered solutions was analyzed histologically, through transmission electron microscopy (TEM) oxidative stress parameters, comparison group. (3) Results: Liver tissue histological evaluation showed non-significant degeneration hepatocytes group, slight periportal inflammation. TEM investigation revealed nuclear mitochondrial polymorphism lipid accumulation irregular nucleus profiles increased glycogen storage cytosol. Oxidative analysis antioxidant effect both treatments. (4) Conclusions: Compared treatments produced significant increases protection, probably by properties. Histological modifications liver minimal groups. unspecific alterations structure. Keywords: chitosan; nanoparticles; injury;

Language: Английский

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Revolutionizing Nerve Repair

Advances in medical diagnosis, treatment, and care (AMDTC) book series, Journal Year: 2024, Volume and Issue: unknown, P. 275 - 300

Published: Oct. 11, 2024

An extensive variety of etiologies, including trauma and chronic health issues, can result in peripheral nerve injuries. These injuries provide significant hurdles regarding clinical care the eventual restoration usual ability. In response to these obstacles, nanoparticles have become a viable therapeutic approach that has potential accelerate regeneration. This chapter includes thorough examination categories synthetic processes related nanoparticles. Here, will illustrate critical role play advancing field regeneration through this comprehensive investigation.

Language: Английский

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