Immunogenicity and Safety of an Intradermal BNT162b2 mRNA Vaccine Booster after Two Doses of Inactivated SARS-CoV-2 Vaccine in Healthy Population

Vaccines, Journal Year: 2021, Volume and Issue: 9(12), P. 1375 - 1375

Published: Nov. 23, 2021

Effective vaccine coverage is urgently needed to tackle the COVID-19 pandemic. Inactivated vaccines have been introduced in many countries for emergency usage, but only provided limited protection. Heterologous vaccination a promising strategy maximise immunogenicity. Here, we conducted phase I, randomised control trial observe safety and immunogenicity after an intradermal boost, using fractional dosage (1:5) of BNT162b2 mRNA healthy participants Songkhla, Thailand. In total, 91 volunteers who had administered with two doses inactivated SARS-CoV-2 (CoronaVac) were recruited into study, then (1:1:1) received different regimens third dose. An intramuscular booster full dose was included as conventional control, half group reciprocal comparator. Both, immediate delayed adverse events following immunisation (AEFI) monitored. Humoral cellular immune responses examined effects. The significantly fewer systemic side effects, from 70% down 19.4% (p < 0.001); however, they comparable local reactions booster. receiving one fifth dosage, serum Anti-RBD IgG halved compared injection. However, neutralising function against Delta strain remained intact. T cell also less effective Together, booster, BNT162b2, can reduce provides good level antibody This favourable boosting suitable alternative management increase during shortage.

Language: Английский

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Design and Evaluation of Synthetic Delivery Formulations for Peptide-Based Cancer Vaccines

BME Frontiers, Journal Year: 2024, Volume and Issue: 5

Published: Jan. 1, 2024

With the recent advances in neoantigen identification, peptide-based cancer vaccines offer substantial potential field of immunotherapy. However, rapid clearance, low immunogenicity, and insufficient antigen-presenting cell (APC) uptake limit efficacy vaccines. This review explores barriers hindering vaccine efficiency, highlights advancements synthetic delivery systems, features strategies for key steps lymph node (LN) drainage, APC delivery, cross-presentation strategies, adjuvant incorporation. paper also discusses design preclinical studies evaluating including administration routes murine tumor models.

Language: Английский

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An Update on mRNA-Based Viral Vaccines

Vaccines, Journal Year: 2021, Volume and Issue: 9(9), P. 965 - 965

Published: Aug. 29, 2021

With the success of COVID-19 vaccines, newly created mRNA vaccines against other infectious diseases are beginning to emerge. Here, we review structural elements required for designing vaccine constructs effective in vitro synthetic transcription reactions. The unprecedently speedy development severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was enabled with previous innovations nucleoside modifications during and lipid nanoparticle delivery materials mRNA. Recent updates briefly described status SARS-CoV-2, influenza virus, viral pathogens. Unique features platforms future perspectives discussed.

Language: Английский

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Detachable-dissolvable-microneedle as a potent subunit vaccine delivery device that requires no cold-chain

Vaccine X, Journal Year: 2023, Volume and Issue: 15, P. 100398 - 100398

Published: Oct. 17, 2023

Although vaccine administration by microneedles has been demonstrated, delivery reliability issues have prevented their implementation. Through an ex vivo porcine skin experiment, we show visual evidence indicating that detachable dissolvable (DDMN) can deposit cargo into the dermis with insignificant loss of to stratum corneum. Using ovalbumin (OVA), a model antigen vaccine, as cargo, experiments yielded efficiency 86.08 ± 4.16 %. At room temperature, OVA could be stabilized for up 35 days in DDMN made from hyaluronic acid and trehalose. The matrix improve denaturation temperature around 70-120 °C over 150 °C, demonstrated differential scanning calorimetric analysis. In mice's via elicited 10 times higher specific antibody responses compared conventional intramuscular injection. We envision effective, precise dosing, intradermal system may require no cold-chain, offers dose-sparing effect, administered easily.

Language: Английский

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Study of the effectiveness of combined rabies-tetanus vaccine as compared to individual vaccines

Clinical and Experimental Vaccine Research, Journal Year: 2025, Volume and Issue: 14

Published: Jan. 1, 2025

Effective treatment for animal bites is essential, encompassing immediate measures and protocols rabies tetanus vaccination. In this study, we evaluated the effectiveness of administration individual combined rabies-tetanus (RT) vaccines in mice as model animals. Animal groups were injected with either undiluted Toxovac® Rabies vaccine® (RT/Group 1)/Speeda® (ST/Group 3), 2-fold diluted (RT1/2/Group 2), or purified toxoid Speeda® (Spurf/Group 4). Mice immunized 2 intraperitoneal (IP) doses at one-week interval one subcutaneous (SC) dose immunogenicity, SC immunogenicity. The potency was determined through challenge test, while their immunogenicity examined by measuring anti-rabies anti-tetanus immunoglobulin G response. All tested potent except Spurf; not potent. Rabies' all combinations both routes showed comparable antibody response & non-significant difference (p≥0.05) days 14 28 compared to single IP doses. Tetanus' Toxovac®. RT depicted higher on 28. Whereas RT1/2 a Therefore, has synergistic effect combination. immune combination vaccine effective vaccine. Our results highlight potential via cost-effective means provide protective immunity.

Language: Английский

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A Promising Needle-Free Pyro-Drive Jet Injector for Augmentation of Immunity by Intradermal Injection as a Physical Adjuvant

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(10), P. 9094 - 9094

Published: May 22, 2023

Current worldwide mRNA vaccination against SARS-CoV-2 by intramuscular injection using a needled syringe has greatly protected numerous people from COVID-19. An is generally well tolerated, safer and easier to perform on large scale, whereas the skin benefit of presence immune cells, such as professional antigen-presenting dendritic cells. Therefore, intradermal considered superior for induction protective immunity, but more proficiency required injection. To improve these issues, several different types versatile jet injectors have been developed deliver DNAs, proteins or drugs high velocity through without needle. Among them, new needle-free pyro-drive injector unique characteristic that utilizes gunpower mechanical driving force, in particular, bi-phasic pyrotechnics provoke consequently wide dispersion injected DNA solution skin. A significant amount evidence revealed it highly effective vaccinating tool induce potent cellular humoral immunity cancers infectious diseases. This presumably explained fact shear stress generated facilitates uptake cells and, consequently, its protein expression. The also possibly elicits danger signals which, together with plasmid DNA, subsequently induces activation innate including cell maturation, leading establishment adaptive immunity. review summarizes recent advances augment possible mechanism action.

Language: Английский

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Immunogenicity and safety of an intradermal ChAdOx1 nCoV-19 boost in a healthy population

npj Vaccines, Journal Year: 2022, Volume and Issue: 7(1)

Published: May 13, 2022

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global pandemic. Two doses of an inactivated SARS-CoV-2 vaccine (CoronaVac) have been shown to be insufficient protect against variants concern (VOCs), while viral vector vaccines remain protective the infection. Herein, we conducted preliminary study evaluate safety and immunity in adult population who received conventional dosage-regimen vaccine; with additional intradermal ChAdOx1 nCoV-19 reciprocal dosage (1:5). An Intramuscular booster was also included as control. Immediate delayed local reactions were frequently observed fractional boost, but systemic side effects significantly decreased compared intramuscular boost. The anti-RBD-IgG levels, neutralising function delta variants, T cell responses increased after boosting via both routes. Interestingly, shorter interval elicited higher immunogenicity extended interval. Taken together, reduces adverse enhances non inferiority humoral cellular immune full dose boosting. These findings provide for effective management during shortages supply.

Language: Английский

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The immunological effects of intradermal particle-based vaccine delivery using a novel microinjection needle studied in a human skin explant model

Vaccine, Journal Year: 2023, Volume and Issue: 41(13), P. 2270 - 2279

Published: March 1, 2023

For intradermal (ID) immunisation, novel needle-based delivery systems have been proposed as a better alternative to the Mantoux method. However, penetration depth of needles in human skin and its effect on immune cells residing different layers has not analyzed. A user-friendly silicon microinjection needle (Bella-muTM) developed, which allows for perpendicular injection due short length (1.4-1.8 mm) ultrashort bevel. We aimed characterize performance this context particle-based outer membrane vesicle (OMV) vaccine using an ex vivo explant model. compared 1.4 1.8 mm with conventional method investigate capacity antigen-presenting cell (APC) phagocytose OMVs. The deposited antigen closer epidermis than or Consequently, activation epidermal Langerhans was significantly higher determined by dendrite shortening. found that five subsets dermal APCs are able OMV vaccine, irrespective device ID OMV-based allowed APC targeting, superior cells. This study indicates use improves vaccines skin.

Language: Английский

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A Call to Action by the Italian Mesotherapy Society on Scientific Research

Drug Design Development and Therapy, Journal Year: 2021, Volume and Issue: Volume 15, P. 3041 - 3047

Published: July 1, 2021

Abstract: Mesotherapy (local intradermal therapy, LIT) is a technique used to slowly spread drugs in tissues underlying the site of injection prolong pharmacological effect with respect intramuscular injection. Recommendations for proper medical use this have been made pain medicine and rehabilitation, chronic venous disease, sport medicine, musculoskeletal disorders, several dermatological conditions, skin ageing, immune-prophylaxis. Although mesotherapy considered valid technique, unresolved questions remain, which should be answered standardize methodology dosing regimen as well define right indications clinical practice. New randomized controlled trials are needed test single products (dose, frequency administration, efficacy safety). Even infiltration substances dermo-cosmetic purposes must guided by safety tests before being proposed mesotherapy. In article, we put forth preclinical research plan health technology assessment call action doctors, researchers scientific societies aid national authorities considering prevention, treatment rehabilitation paths. Keywords: mesotherapy,

Language: Английский

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Immunogenicity and reactogenicity after booster dose with AZD1222 via intradermal route among adult who had received CoronaVac

Vaccine, Journal Year: 2022, Volume and Issue: 40(24), P. 3320 - 3329

Published: May 1, 2022

Language: Английский

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