Commonalities and Disparities between Endometriosis and Chronic Endometritis: Therapeutic Potential of Novel Antibiotic Treatment Strategy against Ectopic Endometrium

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(3), P. 2059 - 2059

Published: Jan. 20, 2023

Chronic endometritis (CE) is a local mucosal inflammatory disorder of the uterine lining, which histopathologically recognized as unusual infiltration CD138(+) plasmacytes into endometrial stromal compartment. Accumulating body research documented that CE associated with female infertility and several obstetric/neonatal complications. The major cause thought to be intrauterine infection represented by common bacteria (Escherichia coli, Enterococcus faecalis, Streptococcus, Staphylococcus), Mycoplasma/Ureaplasma, Mycobacterium. Additionally, dysbiosis in reproductive tract may involved onset development CE. Antibiotic treatments against these microorganisms are effective elimination affected patients. Meanwhile, endometriosis disease characterized endometriotic tissues (ectopic endometrium) growing outside uterus potentially causes chronic pelvic symptoms (dysmenorrhea, dyspareunia, dyschezia, dysuria), infertility, ovarian cancers. Endometriosis involves endocrinological, genetic, epigenetic factors its etiology pathogenesis. Recent studies focus on immunological, inflammatory, infectious aspects demonstrate characteristics between This review aimed better understand immunological microbial backgrounds underlying look therapeutic potential novel antibiotic treatment strategy light disease.

Language: Английский

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Predictive biomarkers for anti-TNF alpha therapy in IBD patients

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: March 16, 2024

Inflammatory bowel disease (IBD) is a chronic gastrointestinal condition characterized by severe gut inflammation, commonly presenting as Crohn's disease, ulcerative colitis or categorized IBD- unclassified. While various treatments have demonstrated efficacy in adult IBD patients, the advent of anti-TNF therapies has significantly revolutionized treatment outcomes and clinical management. These played pivotal role achieving endoscopic remission, promoting mucosal healing, averting progression, diminishing necessity for surgery. Nevertheless, not all patients exhibit positive responses to these therapies, some may experience loss responsiveness over time. This review aims present comprehensive examination predictive biomarkers monitoring therapeutic response therapy patients. It will explore their limitations utilities, paving way more personalized effective approach.

Language: Английский

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Alterations of Vaginal Microbiota and Chlamydia trachomatis as Crucial Co-Causative Factors in Cervical Cancer Genesis Procured by HPV

Microorganisms, Journal Year: 2023, Volume and Issue: 11(3), P. 662 - 662

Published: March 6, 2023

Chlamydia trachomatis and human papillomavirus (HPV) are the most common pathogens found in sexually transmitted infections (STIs), both known to increase risk of cervical cancer (CC) infertility. HPV is extremely worldwide, scientists use it distinguish between low-risk high-risk genotypes. In addition, transmission can occur via simple contact genital area. From 50 80% active individuals become infected with C. viruses during their lifetime, up 50% an oncogenic genotype. The natural history this coinfection strongly conditioned by balance host microbiome immune condition infecting agent. Though infection often regresses, tends persist throughout adult life asymptomatically silently. partnership basically due similarities: routes, reciprocal advantages, same factors. a Gram-negative bacteria, similar HPV, intracellular bacterium, which shows unique biphasic development that helps latter continue its steady progression into entire life. Indeed, depending on individual’s condition, migrate toward upper tract spread uterus, fallopian tubes open pathway invasion. related female facilitated decay first line defense vaginal environment, constituted healthy characterized net equilibrium all components. Thus, aim paper was highlight complexity fragility microenvironment accentuate fundamental role elements systems involved, including Lactobacillus strains (Lactobacillus gasseri, jensenii, crispatus) immune–endocrine system, preserving from mutation. Therefore, age, diet, genetic predisposition together unspecific, persistent low-grade inflammatory state were be implicated high frequency severity grade disease, potentially resulting pre-cancerous cancerous lesions.

Language: Английский

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Deciphering the role of female reproductive tract microbiome in reproductive health: a review

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: March 18, 2024

Relevant studies increasingly indicate that female reproductive health is confronted with substantial challenges. Emerging research has revealed the microbiome interacts anatomy, histology, and immunity of tract, which are cornerstone maintaining preventing adverse pregnancy outcomes. Currently, precise mechanisms underlying their interaction impact on physiological functions tract remain elusive, constituting a prominent area investigation within field microecology. From this new perspective, we explore interactions between factors affect composition in as well personalized medicine approaches managing based microbiome. This study highlights pivotal role influencing occurrence diseases. These findings support exploration innovative for prevention, monitoring treatment diseases

Language: Английский

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Crosstalk between the Resident Microbiota and the Immune Cells Regulates Female Genital Tract Health

Life, Journal Year: 2023, Volume and Issue: 13(7), P. 1531 - 1531

Published: July 9, 2023

The female genital tract (FGT) performs several functions related to reproduction, but due its direct exposure the external environment, it may suffer microbial infections. Both upper (uterus and cervix) lower (vagina) FGT are covered by an epithelium, contain immune cells (macrophages, dendritic cells, T B lymphocytes) that afford a robust protection host. Its part differ in terms of Lactobacillus spp., which dominant vagina. An alteration physiological equilibrium between local microbiota leads condition dysbiosis which, turn, account for outcome infection. Aerobic vaginitis, bacterial vaginosis, Chlamydia trachomatis most frequent infections, can lead severe complications reproduction pregnancy. use natural products, such as probiotics, polyphenols, lactoferrin course infections is issue current investigation. In spite positive results, more research needed define appropriate administration, according type patient.

Language: Английский

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All Properties of Infertility Microbiome in a Review Article

Journal of Clinical Laboratory Analysis, Journal Year: 2025, Volume and Issue: unknown

Published: March 9, 2025

The microbiome is crucial for many physiological processes, including immunity, metabolism, and reproduction. This review aims to contribute a detailed understanding of the genital tract, which can lead better management dysbiosis reproductive disorders. Data from four international information databases Medline, Scopus, Embase, Google Scholar. search strategy was based on combination following terms: "microbiota," "microbiome," "microfilm," "microflora," "fertility," or "infertility." advent next-generation sequencing-based technologies during last decade has revealed presence microbial communities in nearly every part human body, system. Several studies have shown significant differences between microbiota vagina endometrium, as well other parts upper tract. plays critical role determining person's health state, tract may impact fertility potential before after assisted treatments (ARTs). To completely understand microbiome, future research should focus not only description but also interaction bacteria, production biofilms, microorganisms with cells.

Language: Английский

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Antimicrobial Resistance in Maternal Infections During Pregnancy

Biomedicines, Journal Year: 2025, Volume and Issue: 13(4), P. 777 - 777

Published: March 22, 2025

Background: An imbalance in the vaginal microbiota, often characterized by reduced lactobacilli, paves way forth for opportunistic bacteria from gastrointestinal tract. The presence of aerobic genital tract during pregnancy can have negative outcomes on pregnancy. Peripartum infections, when not adequately managed, significantly impact maternal and neonatal health. Antimicrobial resistance poses an escalating global health threat, with newborns particularly vulnerable. Methods: This study constitutes a retrospective observational analysis, encompassing all microbial strains isolated pregnant women admitted to “Pius Brînzeu” Clinical County Emergency Hospital Timișoara, Romania various infectious diseases over one year. We analyzed 274 samples 246 women, which 242 were cervical samples, 23 urine cultures, 3 wound secretions, amniotic fluids, 1 peritoneal cavity fluid, sputum, hemoculture. Results: In Group B Streptococcus (GBS) was most prevalent, representing 42.46% isolates. E. coli second frequent at 30.16%, followed K. pneumoniae 11.9%, S. aureus 8.73%, C. albicans 2.78%, other species 3.97%. A total 9.63% GBS isolates exhibited penicillin, while 23.36% identified as multi-drug resistant (MDR). Methicillin-resistant (MRSA) MDR 50% 54.54% aureus-positive respectively. Conclusions: Recognizing implications infection or colonization, especially antimicrobial bacteria, aids assessing risks

Language: Английский

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Pato: prediction of probiotic bacteria using metabolic features

Brazilian Journal of Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 23, 2025

Language: Английский

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Changes in microbial composition and interaction patterns of female urogenital tract and rectum in response to HPV infection

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: Feb. 1, 2024

Abstract Background Previous studies have shown that changes in the microbial community of female urogenital tract are associated with Human papillomavirus (HPV) infection. However, research on this association was mostly focused a single site, and there currently few joint HPV infection multiple sites tract. Methods We selected 102 healthy women from Yunnan Province as object, collected cervical exfoliation fluid, vaginal, urethral, rectal swabs for analysis, measured bacterial load, related cytokine content. The link between HPV, microbiota, inflammation comprehensively evaluated using bioinformatics methods. Findings impact composition different parts varies. identified several signature genera respond to detection sites, such Corynebacterium , Lactobacillus Campylobacter Cutibacterium been detected reflecting their potential significance cross body responses. There solid interaction network cervix, vagina, urethra. interrelationships inflammatory factors might also affect immune system's response Interpretation It be an effective strategy prevent treat by simultaneously understanding correlation rectum infection, controlling parts.

Language: Английский

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Optimal sampling and analysis methods for clinical diagnostics of vaginal microbiome

European Journal of Clinical Microbiology & Infectious Diseases, Journal Year: 2023, Volume and Issue: 42(2), P. 201 - 208

Published: Jan. 10, 2023

Next-generation sequencing-based microbiological analysis is a complex way to profile vaginal microbiome samples since each step affects the results gained. Methodologies for sample collection lack golden standards. We compared Puritan DNA/RNA swab (PS) and Copan FLOQ (CS) provided consistent reliable profiles analyzed by 16S rRNA gene sequencing. collected two consecutive utilizing PS with room temperature storing CS instant freezing from 26 women. Variable region 4 of bacterial was amplified single PCR custom-designed dual-indexed primers sequenced Illumina MiSeq system. Read quality control, operational taxonomic unit tables, alpha beta diversities were performed, community richness, diversity, evenness evaluated between samplings tests. Nineteen pairs produced detectable, intact DNA during extraction protocol and/or further microbial profiles. Alpha diversity indices independent on protocol. No significant statistical differences found in measured metrics methods. Of women, 43% had Lactobacillus-dominated despite method. Previously reported important phyla Actinobacteria, Bacteroidetes, Firmicutes, Fusobacteria, Proteobacteria present set although their relative abundances varied among individuals. enable constant microbiota sampling. The method no need suitable on-site collections at clinics. Furthermore, it seems be possible take instead one results.

Language: Английский

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