A bioengineered model for reinnervating the decellularized extracellular matrix of corneal scaffolds

Medical Hypotheses, Journal Year: 2024, Volume and Issue: 185, P. 111315 - 111315

Published: Feb. 29, 2024

Corneal diseases and injuries, with their substantial global prevalence adverse effects on quality of life, demographics, occupations, lifestyles, pose a pressing healthcare challenge worldwide. Limited treatment options are unable to halt the progression end-stage conditions, where transplantation is ideal solution. Unfortunately, high demand low supply corneal tissues, donor-recipient mismatches, host rejections leading graft failure limit this option. As result, there critical need for alternative interventions. This article aims establish bioengineered model generated from cadaveric decellularized extracellular matrix (dECM) that can repurpose discarded human tissues potentially increase transplantable tissues. Comparable studies have primarily focused reendothelialization re-epithelialization. Therefore, we hypothesize devising method support scaffold reinnervation emanating prominent nerve plexi spanning stroma epithelium in patient-centered manner, using peripheral blood mononuclear cells.

Language: Английский

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A sustainable approach to derive sheep corneal scaffolds from stored slaughterhouse waste

Regenerative Medicine, Journal Year: 2024, Volume and Issue: 19(6), P. 303 - 315

Published: June 2, 2024

The escalating demand for corneal transplants significantly surpasses the available supply. To bridge this gap, we concentrated on ethical and sustainable grafting sources. Our objective was to create viable scaffolds from preserved slaughterhouse waste.

Language: Английский

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A proposed model of xeno-keratoplasty using 3D printing and decellularization

Frontiers in Pharmacology, Journal Year: 2023, Volume and Issue: 14

Published: Sept. 20, 2023

Corneal opacity is a leading cause of vision impairment and suffering worldwide. Transplantation can effectively restore reduce chronic discomfort. However, there considerable shortage viable corneal graft tissues. Tissue engineering may address this issue by advancing xeno-keratoplasty as alternative to conventional keratoplasty. In particular, livestock decellularization strategies offer the potential generate bioartificial ocular prosthetics in sufficient supply match existing projected needs. To end, we have examined best practices characterizations that supported current state-of-the-art driving preclinical clinical applications. Identifying challenges delimit activities supplement donor pool derived from acellular scaffolds allowed us hypothesize model for keratoprosthesis applications combining 3D printing decellularization.

Language: Английский

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Transforming meat waste into sustainable corneal keratoplasty models

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: April 14, 2025

With a rapidly global population, there is critical need to enhance food production and waste management. This necessity driving opportunities for sustainable integrated chains committed biovalorization circular bioeconomic practices. One approach that aligns with this vision relies on tissue engineering, which offers leverage systems in the search natural biomaterials from agricultural waste. In perspective, we propose utilizing common meat sources, often associated high environmental footprint, develop graft models. These models reduce waste, decrease reliance animal testing, support both medical innovation. Specifically, explore unique generate corneal transplantation completely discarded components of chain, using eyes bladders. strategy involves creating keratoplasty by reseeding decellularized extracellular matrix (dECM), encompassing three major regions: epithelium, stroma, endothelium. Interestingly, these scaffolds can be recellularized cellular lineages derived stem niches harvested urine. integrates management regenerative medicine, fostering advancements engineering.

Language: Английский

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Integrated environmental and health economic assessments of novel xeno-keratografts addressing a growing public health crisis

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Oct. 27, 2024

Tissue scarcity poses global challenges for corneal transplantation and public health. Xeno-keratoplasty using animal-derived tissues offers a potential solution, but its environmental economic implications remain unclear. This study evaluated two xeno-keratoplasty procedures at single institution: (1) native corneas (Option 1) (2) tissue-engineered scaffolds derived from slaughterhouse waste 2). Life cycle assessment (LCA) quantified impacts across 18 midpoint indicators, while cost-effectiveness analysis (CEA) incorporated cost impact approaches. Option 1 exhibited significantly lower than 2 most primarily due to the energy equipment demands of cell culture in 2. Both CEA approaches (carbon offset pricing utility decrement) demonstrated dominance 1. appears more environmentally economically favorable 2) current analysis. Future studies could explore diverse techniques optimizing sustainability.

Language: Английский

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Investigation of Fatty Acid Profile of Eyes Recovered from Slaughterhouse Waste

Heliyon, Journal Year: 2024, Volume and Issue: 10(18), P. e38148 - e38148

Published: Sept. 1, 2024

Polyunsaturated fatty acids (PUFAs), principally Docosahexaenoic acid (DHA, 22:6n-3), the foremost omega-3 PUFAs in brain and eyes, have been implicated maintaining structural functional properties of retina cornea. Another PUFA, Arachidonic Acid (AA, 20:4n-6), primary omega-6 PUFA cell membrane phospholipids, is a central inflammatory mediator involved many molecular cellular functions under physiological pathological conditions, including dry eye disease (DED) age-related macular degeneration (AMD). This study investigated (FA) composition vitreous humor, retina, cornea, whole two mammals, Arabian sheep (

Language: Английский

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