Mechanism and Application Prospects of NLRC3 Regulating cGAS-STING Pathway in Lung Cancer Immunotherapy DOI Creative Commons
Qichao Wang, Zhen Ren,

Jianing Zhao

et al.

International Journal of Medical Sciences, Journal Year: 2024, Volume and Issue: 21(13), P. 2613 - 2622

Published: Jan. 1, 2024

NLRC3, a negative regulator, exhibits considerable potential in the realm of lung cancer immunotherapy by virtue its profound impact on immune response intensity, primarily through regulatory effects cGAS-STING pathway. The inhibition NLRC3 has been found to augment activity aforementioned pathway, thereby enhancing anti-tumor response. This comprehensive review endeavors elucidate molecular and genetic structures role within system, interaction with particular emphasis applications immunotherapy. Existing research underscores NLRC3's capacity mitigate excessive responses via regulation thus underscoring significant development pharmaceutical interventions gene therapy strategies targeting presents promising avenue for creation novel therapeutic options individuals afflicted cancer. Nonetheless, clinical application these therapies is confronted both technical biological challenges. aims provide theoretical foundation related delineate future directions this field.

Language: Английский

Exploring the Mechanism of Ferroptosis Induction by Sappanone A in Cancer: Insights into the Mitochondrial Dysfunction Mediated by NRF2/xCT/GPX4 Axis DOI Creative Commons

Junyan Wang,

Haowen Zhuang,

Xiaocui Yang

et al.

International Journal of Biological Sciences, Journal Year: 2024, Volume and Issue: 20(13), P. 5145 - 5161

Published: Jan. 1, 2024

Non-small cell lung cancer (NSCLC), a major subtype of cancer, encompasses squamous carcinoma, adenocarcinoma, and large carcinoma. Compared to small NSCLC cells grow divide more slowly, their metastasis occurs at later stage. Currently, chemotherapy is the primary treatment for this disease. Sappanone A (SA) flavonoid compound extracted from plant Caesalpinia sappan, known its antitumor, redox-regulating, anti-inflammatory properties. Recent studies have investigated interaction SA with mitochondrial pathways in regulating death through Nrf-2/GPX-4/xCT axis. This study specifically explores mechanism by which affects morphology structure regulation mitophagy biogenesis tumor cells. The primarily utilizes second-generation transcriptomic sequencing data molecular docking techniques elucidate role programmed omics results indicate that significantly targets genes involved oxidative phosphorylation, mitophagy, dynamics, stress. Further findings confirmed Nrf-2/GPX4/xCT pathway serves as crucial target NSCLC. Knockdown Nrf-2 (si-Nrf-2) overexpression (ad-Nrf-2) were shown modulate therapeutic efficacy varying degrees. Additionally, modifications GPX4/xCT affected regulatory effects on autophagy, biogenesis, energy metabolism. These mechanisms may be mediated caspase ferroptosis-related signaling. Molecular biology experiments demonstrated intervention further inhibits phosphorylation FUNDC1 Tyr18 downregulates TOM20 expression. was found reduce expression PGC1α, Nrf-1, Tfam, resulting decrease respiration Overexpression counteract biogenesis. Confocal microscopy revealed increases fragmentation, subsequently inducing pathway-mediated death. However, genetic modification altered In conclusion, has been identified promising agent apoptosis ferroptosis represent key Targeting axis offers novel approach maintaining homeostasis within cellular microenvironment.

Language: Английский

Citations

17
Targeting regulated cell death: Apoptosis, necroptosis, pyroptosis, ferroptosis, and cuproptosis in anticancer immunity DOI
Ziyu Guo,

Yihuang Liu,

Danyao Chen

et al.

Journal of Translational Internal Medicine, Journal Year: 2025, Volume and Issue: 13(1), P. 10 - 32

Published: Feb. 1, 2025

In the evolving landscape of cancer treatment, strategic manipulation regulated cell death (RCD) pathways has emerged as a crucial component effective anti-tumor immunity. Evidence suggests that tumor cells undergoing RCD can modify immunogenicity microenvironment (TME), potentially enhancing its ability to suppress progression and metastasis. this review, we first explore mechanisms apoptosis, necroptosis, pyroptosis, ferroptosis, cuproptosis, along with crosstalk between these modalities. We then discuss how processes activate antigen-presenting cells, facilitate cross-priming CD8+ T trigger immune responses, highlighting complex effects novel forms on TME biology. Furthermore, summarize potential drugs nanoparticles induce or inhibit emerging their therapeutic roles in treatment. Finally, put forward existing challenges future prospects for targeting anti-cancer Overall, review enhances our understanding molecular biological impacts RCD-based therapies, providing new perspectives strategies

Language: Английский

Citations

1
Prognostic prediction of m6A and ferroptosis-associated lncRNAs in liver hepatocellular carcinoma DOI Creative Commons
Yuchen Gao, Jingxiao Li, Mingyue Ma

et al.

Journal of Translational Internal Medicine, Journal Year: 2024, Volume and Issue: 12(5), P. 526 - 529

Published: Nov. 1, 2024

Language: Английский

Citations

7
WWP2 protects against sepsis-induced cardiac injury through inhibiting cardiomyocyte ferroptosis DOI Creative Commons
Zhi Li, Boquan Wu, Jie Chen

et al.

Journal of Translational Internal Medicine, Journal Year: 2024, Volume and Issue: 12(1), P. 35 - 50

Published: Feb. 1, 2024

Abstract Background and Objectives Cardiac injury plays a critical role in contributing to the mortality associated with sepsis, condition marked by various forms of programmed cell deaths. Previous studies hinted at WW domain-containing E3 ubiquitin protein ligase 2 (WWP2) involving heart failure endothelial injury. However, precise implications WWP2 sepsis-induced cardiac injury, along underlying mechanisms, remain enigmatic. Methods Sepsis induced were constructed intraperitoneal injection lipopolysaccharide. To discover function during this process, we designed performed loss/gain-of-function cardiac-specific vectors knockout mice. Combination experiments investigate relationship between downstream signaling septic myocardium Results The level was downregulated cardiomyocytes sepsis. Cardiac-specific overexpression protected from sepsis mitochondrial oxidative stress, death while knockdown or exacerbated process. protective potency predominantly linked its ability suppress cardiomyocyte ferroptosis rather than apoptosis. Mechanistically, our study revealed direct interaction acyl-CoA synthetase long-chain family member 4 (FACL4), through which facilitated ubiquitin-dependent degradation FACL4. Notably, observed notable reduction within mice after FACL4 Conclusions assumes safeguarding against via regulating inhibit LPS-induced ferroptosis.

Language: Английский

Citations

6
Mitochondrial Stress as a Central Player in the Pathogenesis of Hypoxia-Related Myocardial Dysfunction: New Insights DOI Creative Commons

Zhijiang Guo,

Yingjie Tian, Nanyang Liu

et al.

International Journal of Medical Sciences, Journal Year: 2024, Volume and Issue: 21(13), P. 2502 - 2509

Published: Jan. 1, 2024

Hypoxic injury is a critical pathological factor in the development of various cardiovascular diseases, such as congenital heart disease, myocardial infarction, and failure. Mitochondrial quality control essential for protecting cardiomyocytes from hypoxic damage. Under conditions, disruptions mitochondrial homeostasis result excessive reactive oxygen species (ROS) production, imbalances dynamics, initiate processes including oxidative stress, inflammatory responses, apoptosis. Targeted interventions to enhance control, coenzyme Q10 statins, have shown promise mitigating hypoxia-induced dysfunction. These treatments offer potential therapeutic strategies hypoxia-related diseases by regulating fission fusion, restoring biogenesis, reducing ROS promoting mitophagy.

Language: Английский

Citations

6
PANoptosis-related genes function as efficient prognostic biomarkers in colon adenocarcinoma DOI Creative Commons
Yang Liu,

Yizhao Wang,

Huijin Feng

et al.

Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15

Published: March 4, 2024

Background PANoptosis is a newly discovered cell death type, and tightly associated with immune system activities. To date, the mechanism, regulation application of in tumor largely unknown. Our aim to explore prognostic value PANoptosis-related genes colon adenocarcinoma (COAD). Methods Analyzing data from The Cancer Genome Atlas-COAD (TCGA-COAD) involving 458 COAD cases, we concentrated on five pathways Molecular Signatures Database (MSigDB) comprehensive set immune-related genes. approach involved identifying distinct genetic subtype clusters developing model based these parameters. Results research successfully identified two COAD, marked by profiles gene expression. A model, incorporating findings, demonstrated significant predictive power for survival outcomes, underscoring interplay between responses COAD. Conclusion This study enhances our understanding COAD’s framework, emphasizing synergy system. development insights offers promising tool personalized treatment strategies. Future should focus validating refining this clinical settings optimize therapeutic interventions

Language: Английский

Citations

4
TOM40 as a prognostic oncogene for oral squamous cell carcinoma prognosis DOI Creative Commons
Lifei Deng, Ran Hong, Dunhui Yang

et al.

BMC Cancer, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 15, 2025

To investigate the role of translocase outer mitochondrial membrane 40 (TOM40) in oral squamous cell carcinoma (OSCC) with aim identifying new biomarkers or potential therapeutic targets. TOM40 expression level OSCC was evaluated using datasets downloaded from The Cancer Genome Atlas (TCGA), as well clinical data. correlation between and clinicopathological parameters survival were analyzed TCGA. signaling pathways associated identified through gene set enrichment analysis. A network genes co-expressed constructed functionally annotated by ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway analyses. immune infiltration pattern TCGA-OSCC cohort CIBERSORT algorithm. Clinically significant factors screened levels a clinically relevant nomogram constructed. divided into TOM40high TOM40low groups sensitivity to frequently used chemotherapeutic drugs evaluated. CCK-8 colony formation assays applied determine growth. highly expressed tissues correlated negatively overall (P < 0.05). Patients high showed worse prognosis. Furthermore, GO KEGG analyses differentially related that these are mainly immunity tumorigenesis. Immunological analysis has found proportions several cells. Moreover, we knockdown inhibited growth lines. Our results uncovered is reliable prognostic marker target OSCC.

Language: Английский

Citations

0
EBF1-induced CSRP2 boosts the progression of B-cell acute lymphocytic leukemia by inhibiting ferroptosis DOI Creative Commons
Chengcheng Liu, Gexiu Liu,

Fenling Zhou

et al.

Cancer Letters, Journal Year: 2025, Volume and Issue: 614, P. 217556 - 217556

Published: Feb. 12, 2025

Language: Английский

Citations

0
Identification of potential diagnostic targets and therapeutic strategies for anoikis-related biomarkers in lung squamous cell carcinoma using machine learning and computational virtual screening DOI Creative Commons
Xin Zhang, Jing Zou,

Jinghua Ning

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 14, 2025

Objective Lung squamous cell carcinoma (LUSC) is a common subtype of non-small lung cancer (NSCLC) characterized by high invasiveness, metastatic potential, and drug resistance, resulting in poor patient prognosis. Anoikis, specific form apoptosis triggered detachment from the extracellular matrix (ECM), plays crucial role tumor metastasis. Resistance to anoikis key mechanism which cells acquire potential. Although several studies have identified biomarkers related LUSC, anoikis-related genes (ARGs) remains largely unexplored. Methods Anoikis-related were obtained Harmonizome GeneCards databases, 222 differentially expressed (DEGs) LUSC via differential expression analysis. Univariate Cox regression analysis 74 ARGs significantly associated with survival, prognostic model comprising 8 was developed using LASSO multivariate analyses. The internally validated receiver operating characteristic (ROC) curves Kaplan-Meier (K-M) survival curves. Differences immune infiltration gene between high- low-risk groups analyzed. Virtual screening molecular dynamics simulations performed evaluate therapeutic potential CSNK2A1, model. Finally, vitro experiments conducted validate effects on LUSC. Results 8-gene demonstrated excellent predictive performance stability. Significant differences microenvironment characteristics observed groups, suggesting critical shaping landscape Dihydroergotamine as having highest binding affinity for CSNK2A1. Molecular confirmed that CSNK2A1-Dihydroergotamine complex exhibited strong Further inhibited viability, migration, invasion, downregulated CSNK2A1 expression. Conclusion This study first construct an highlighting its providing insights into personalized therapy. significant anti-LUSC activity holds promise agent. emerged robust candidate early diagnosis target

Language: Английский

Citations

0
Decoding tumor angiogenesis: pathways, mechanisms, and future directions in anti-cancer strategies DOI Creative Commons
Xiaohong Liu, Juan Zhang, Ting Yi

et al.

Biomarker Research, Journal Year: 2025, Volume and Issue: 13(1)

Published: April 18, 2025

Abstract Angiogenesis, a crucial process in tumor growth and metastasis, necessitates targeted therapeutic intervention. This review reviews the latest knowledge of anti-angiogenesis targets tumors, with emphasis on molecular mechanisms signaling pathways that regulate this process. We emphasize microenvironment's role angiogenesis, examine endothelial cell metabolic changes, evaluated potential strategies targeting vascular system. At same time, we analyzed pathway mechanism angiogenesis detail. In addition, paper also looks at development trend drugs, including their future direction challenges, aiming to provide prospective insight into field. Despite potential, anti-angiogenic therapies encounter challenges like drug resistance side effects, necessitating ongoing research enhance cancer treatment efficacy these therapies.

Language: Английский

Citations

0