SPP1 is a plasma biomarker associated with the dia gnosis and prediction of prognosis in sepsis DOI Creative Commons
Yi Shen, Wei Xiong, Ying Hu

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 8, 2024

In this study, peripheral whole blood samples from 22 hospitalized patients and 10 healthy individuals were analyzed using a combination of Data Independent Acquisition (DIA) Enzyme-Linked Immunosorbent Assay (ELISA) techniques to identify differentially expressed proteins (DEPs) in sepsis patients' plasma. The aim was provide accurate detailed biomarkers, such as SPP1, for determining the pathological stages sepsis. known osteopontin1 is pleiotropic protein with wide distribution multifunctional effects. Its expression associated inflammatory changes, including variations levels infectious diseases, allergic situations involving tissue damage. registration number ChiCTR1900021261.The full date first year 2018. affiliated hospital southwest medical university, January 2019 September 2020 normal selected DIA-based quantitative proteomics analysis. addition gene ontology analysis Kyoto Encyclopedia genes genomes analysis, enrichment data performed target network screened through joint protein-protein interaction visualization techniques. targets then validated by Elisa kit. software used analyze differences comparing control group group, well between survivals non-survivals, ROC curve drawn evaluate diagnostic value prognostic effect method corresponding proteins. A total 174 DEPs bioinformatics An go pathway revealed following: These mainly involved biological processes among them are inflammation response, metabolism extracellular matrix, secretion cell secretions, activation cells, immune response. According Kegg they complement cascade polymerization, protease glycosylase activation, synthesis process, biotin metabolism, leukocyte transmembrane migration, bacterial infection phagosome formation. SPP1 identified possible plasma biomarker therefore further Elisa. As result experiments, it has been demonstrated that level significantly compared also higher non-survivals can be see diagnose more accurately improve ability prediction. Cell experiments confirm highly There significant difference group; not only good significance sepsis, but provides reference patient prognosis; Therefore, likely become marker over time.

Language: Английский

Study on gene expression in the liver at various developmental stages of human embryos DOI Creative Commons

Hanqing Chen,

Tingting Li, Ming Cai

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 12

Published: Jan. 8, 2025

The normal development of the liver during human embryonic stages is critical for functionality adult liver. Despite this, essential genes, biological processes, and signal pathways that drive in embryos remain poorly understood. In this study, samples were collected from at progressive developmental stages, ranging 2-month-old to 7-month-old. Highly expressed genes their associated enrichment processes various identified through transcriptomic sequencing. findings indicated with humoral immune responses B-cell-mediated immunity highly early stages. Concurrently, numerous related vitamin response, brown adipocyte differentiation, T cell hormone secretion, hemostasis, peptide steroid metabolism, hematopoietic regulation exhibited increased expression aligned development. Our results suggest may possess multiple functions beyond serving roles. Moreover, study elucidated complex regulatory interactions among involved lymphocyte hemopoiesis, Consequently, necessitates synergistic genes. Notably, alongside conventionally recognized previously uncharacterized function also identified. These establish a foundation future research on diseases arising fetal abnormalities.

Language: Английский

Citations

1
Therapeutic Effects of Anti-Inflammatory and Anti-Oxidant Nutritional Supplementation in Retinal Ischemic Diseases DOI Open Access
Deokho Lee, Zhongjie Fu, Ann Hellström

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(10), P. 5503 - 5503

Published: May 18, 2024

Appropriate nutrients are essential for cellular function. Dietary components can alter the risk of systemic metabolic diseases, including cardiovascular cancer, diabetes, and obesity, also affect retinal age-related macular degeneration, diabetic retinopathy, glaucoma. have been assessed prevention or treatment ischemic diseases aging. In this article, we review clinical experimental evidence concerning potential some nutritional supplements to prevent treat provide further insights into therapeutic effects supplementation on retinopathies. We will roles in preventing protecting against diseases.

Language: Английский

Citations

5
Neuroprotective effects of resveratrol on retinal ganglion cells in glaucoma in rodents: A narrative review DOI Creative Commons
Maryam Golmohammadi,

Seyed Arash Aghaei Meibodi,

Sulieman Ibraheem Shelash Al‐Hawary

et al.

Animal Models and Experimental Medicine, Journal Year: 2024, Volume and Issue: 7(3), P. 195 - 207

Published: May 29, 2024

Abstract Glaucoma, an irreversible optic neuropathy, primarily affects retinal ganglion cells (RGC) and causes vision loss blindness. The damage to RGCs in glaucoma occurs by various mechanisms, including elevated intraocular pressure, oxidative stress, inflammation, other neurodegenerative processes. As the disease progresses, of leads loss. Therefore, protecting from promoting their survival are important goals managing glaucoma. In this regard, resveratrol (RES), a polyphenolic phytoalexin, exerts antioxidant effects slows down evolution progression present review shows that RES plays protective role cases ischemic injury hypoxia as well ErbB2 protein expression retina. Additionally, roles cell growth, reducing apoptosis, decreasing stress H 2 O ‐exposed RGCs. was also found inhibit suppress activation mitogen‐activated kinase signaling pathways. could alleviate function impairment suppressing hypoxia‐inducible factor‐1 alpha/vascular endothelial growth factor p38/p53 axes while stimulating PI3K/Akt pathway. might exert potential therapeutic for survival.

Language: Английский

Citations

4
Expression of Osteopontin in M2 and M4 Intrinsically Photosensitive Retinal Ganglion Cells in the Mouse Retina DOI Creative Commons

Leonie Kinder,

Moritz Lindner

Investigative Ophthalmology & Visual Science, Journal Year: 2025, Volume and Issue: 66(2), P. 14 - 14

Published: Feb. 5, 2025

Purpose: Melanopsin-expressing intrinsically photosensitive (ip) retinal ganglion cells (RGCs) can be divided into six different subtypes (M1 – M6). Yet, specific markers exist for only some of these that could employed to study the function individual subtypes. Osteopontin (Spp1) marks αRGC, suggesting that, across ipRGCs, it would mark M4-ipRGC subtype (synonymous ON-sustained αRGCs). Recent evidence suggests osteopontin expression spread other ipRGC Therefore, this aims characterize pattern in mice. Methods: Single-cell RNA (scRNA-seq) sequencing data from murine RGCs were analyzed identify patterns Spp1 ipRGCs. Immunohistochemistry (IHC) was performed on cryosections and flatmounts C57BL/6J mice localization Neurite tracing dendritic morphology Results: scRNA-seq analysis revealed two distinct clusters IHC confirmed colocalization with neurofilament heavy chain, an established marker αRGCs, including M4-ipRGCs. immunoreactivity moreover identified one additional group By stratification, those clearly as M2-ipRGCs. Conclusions: Our findings demonstrate is expressed both M2- M4-ipRGCs, challenging notion a exclusively αRGCs. double-labeling melanopsin provides novel method differentiate M2 ipRGCs This will support physiology -specific manner may, instance, foster research field optic nerve injury.

Language: Английский

Citations

0
Understanding Vitamin C: Comprehensive Examination of Its Biological Significance and Antioxidant Properties DOI Creative Commons
Abdulsamed Kükürt, Volkan Gelen

IntechOpen eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 5, 2024

Vitamin C, an essential water-soluble vitamin, is known for its pivotal role in various biological functions. This chapter provides overview of vitamin focusing on chemical structure and synthesis, multifaceted functions within the body, remarkable as a powerful antioxidant. The significance C maintaining immune function, contributions to collagen involvement cognitive health are explored. Moreover, dual nature both pro-oxidant antioxidant highlighted, emphasizing broad impact well-being. comprehensive examination underscores critical safeguarding against oxidative damage-related diseases supporting overall health.

Language: Английский

Citations

3
Increased Vitamin C Intake Is Associated With Decreased Pancreatic Cancer Risk DOI

Maria Pereira,

Matthew Cardeiro,

Lexi Frankel

et al.

World Journal of Oncology, Journal Year: 2024, Volume and Issue: 15(4), P. 543 - 549

Published: July 6, 2024

Patients with pancreatic cancer have an unfavorable 5-year survival rate of approximately 3% due to diagnosis occurring at advanced stages. Prior research has proposed vitamin C may a therapeutic and preventative role in cancer.

Language: Английский

Citations

1
Glaucoma and dietary links: insights from high-salt intake, the Mediterranean diet, and specific nutrients DOI Creative Commons
Yuqi Yang, Hongyan Zhou, Hong Zhang

et al.

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: Oct. 24, 2024

Glaucoma, a prevalent and potentially blinding eye disease, is linked to variety of factors, including elevated intraocular pressure, optic nerve damage, oxidative stress. In recent years, dietary habits, as controllable lifestyle factor, have received increasing attention in the prevention treatment glaucoma. The purpose this review was investigate effects factors on glaucoma, with particular emphasis two common patterns: high-salt diet Mediterranean diet. addition, we investigated association between many nutrients (including omega-3 fatty acids, vitamins, caffeine, minerals) glaucoma fully assess potential involvement variables pathogenesis, prevention, treatment. This article reveals importance components explores prospective possibilities for future research by conducting comprehensive previous scientific studies.

Language: Английский

Citations

1
Expression of Osteopontin in M2 and M4 intrinsically photosensitive retinal ganglion cells DOI Creative Commons

Leonie Kinder,

Moritz Lindner

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 31, 2024

Abstract Purpose Melanopsin-expressing intrinsically photosensitive (ip) retinal ganglion cells (RGC) can be divided into six different subtypes (M1 – M6). Yet, only for some of these specific markers exist that could employed to study the function individual subtypes. Osteopontin ( Spp1 ) marks alpha(α)-RGC, suggesting that, across ipRGC, it would mark M4-ipRGC subtype (synonymous to: ON-sustained-αRGC). Recent evidence suggests osteopontin expression spread other ipRGC Therefore, this aims characterise pattern Methods Single-cell RNA sequencing data from RGC were analysed identify patterns ipRGC. Immunohistochemistry (IHC) was performed on cryosections and flatmounts C57BL/6J mice characterize localization Neurite tracing dendritic morphology Results scRNAseq analysis revealed in two distinct clusters IHC confirmed co-localization with Smi-32, an established marker αRGC, including M4-ipRGCs. immunoreactivity moreover identified one additional group By stratification those clearly as M2-ipRGC. Conclusions Our findings demonstrate is expressed both M2- M4-ipRGC, challenging notion a exclusively αRGC. double-labelling melanopsin provides novel method differentiate M2 This will support physiology manner may instance foster research field optic nerve injury.

Language: Английский

Citations

1
Retinal degenerative diseases: role of dietary supplements in retinal health DOI Creative Commons

Dr.Gautam Kumar,

Priti Talwar

Discover Food, Journal Year: 2024, Volume and Issue: 4(1)

Published: Dec. 5, 2024

Retinal degeneration is a progressive condition that affects the retina, light-sensitive layer, and deteriorates inner retinal cells encompass pigment epithelium, photoreceptors, ganglion are essential for eyesight. Multiple factors including aging, genetic factors, oxidative stress, inflammation, environmental can contribute to degeneration. seen predominantly in Age-related Macular degeneration, Diabetic retinopathy, Glaucoma, Retinitis pigmentosa leading cause of vision loss. The search potential effective therapeutic interventions management has led increased interest dietary supplements, particularly those rich antioxidant properties. Dietary supplements have been proposed as an ideal source preventing especially containing significant levels antioxidants help reduce damage cells. Notably, with carotenoids, flavonoids, alkaloids, amino acids, fatty vitamins, minerals shown positive effects both preclinical clinical studies by preserving structure, revitalizing health, delaying disease progression, improving visual function. To explore this topic further, systematic strategy was employed recent focused on cell lines, animal models, human subjects. Ultimately, review highlights should be used adjunctive therapies alongside comprehensive medical management, aiming maximize their benefits treating

Language: Английский

Citations

1
Neuroprotective effects of resveratrol on retinal ganglion cells in glaucoma: Focusing on oxidative stress, cellular, and molecular mechanisms DOI Open Access
Mohammad Yasin Zamanian, Maryam Golmohammadi,

Seyed Arash Aghaei Meibodi

et al.

Authorea (Authorea), Journal Year: 2024, Volume and Issue: unknown

Published: March 11, 2024

Retinal ganglion cells (RGCs) are the major that damaged in glaucoma leading to vision loss and blindness. The damage RGCs is caused by various mechanisms such as elevated intraocular pressure, oxidative stress, inflammation, other neurodegenerative processes. As disease progresses, more lost, a gradual of vision. Therefore, protecting from promoting their survival an important goal treatment glaucoma. Resveratrol (RES) exerts anti-oxidant effects slows down evolution progression This review was prepared using databases Google Scholar, PubMed, Scopus, ScienceDirect. Based on findings this review, RES has protective role cases ischemic injury hypoxia well ErbB2 protein expression retina. Additionally, cell growth, reducing apoptosis, decreasing stress H2O2-exposed RGCs. also found inhibit suppress activation MAPK signaling pathways. Moreover, could alleviate retinal function impairment suppressing HIF-1a/VEGF p38/p53 axis while stimulating PI3K/Akt pathway. Hence, might exert potential therapeutic survival.

Language: Английский

Citations

0