Frontiers in Microbiology,
Journal Year:
2024,
Volume and Issue:
14
Published: Jan. 8, 2024
Bacteriocins
are
antimicrobial
peptides
applied
in
food
preservation
and
interesting
candidates
as
alternatives
to
conventional
antibiotics
or
microbiome
modulators.
Recently,
we
established
Corynebacterium
glutamicum
a
suitable
production
host
for
various
bacteriocins
including
garvicin
Q
(GarQ).
Here,
establish
secretion
of
GarQ
by
C.
via
the
Sec
translocon
achieving
titers
about
7
mg
L
–1
initial
fermentations.
At
neutral
pH,
cationic
peptide
is
efficiently
adsorbed
negatively
charged
envelope
producer
bacteria
limiting
availability
bacteriocin
culture
supernatants.
A
combination
CaCl
2
Tween
80
reduces
adsorption
.
Moreover,
cultivation
minimal
medium
supplemented
with
improves
15
but
resulted
reduced
activity
at
later
timepoints.
Using
reporter
strain
proteomic
analyses,
identified
HtrA,
protease
associated
stress,
another
potential
factor
production.
Transferring
HtrA-deficient
K9
close
40
Applying
conditions
low
aeration
prevented
loss
timepoints
improved
100
This
50-fold
higher
than
previously
shown
employing
native
transporter
GarCD
range
levels
observed
Lactococcus
petauri
B1726.
Additionally,
tested
several
synthetic
variants
were
able
show
that
exchange
methionine
position
5
phenylalanine
(GarQ
M5F
)
results
markedly
increased
against
lactis
Listeria
monocytogenes
In
summary,
our
findings
shed
light
on
aspects
recombinant
may
also
be
relevance
natural
producers
other
bacteriocins.
Foods,
Journal Year:
2023,
Volume and Issue:
13(1), P. 124 - 124
Published: Dec. 29, 2023
The
interest
in
microalgae
as
food
Europe
is
growing
due
to
its
remarkable
features
that
can
foster
a
sustainable
economy.
lack
of
tradition
on
their
use
among
Europeans
changing
and
demand
for
more
products
increasing.
legal
framework
from
the
stakeholders’
point
view
has
been
consistently
identified
bottleneck,
regardless
nutritional
value
potential
provide
added-value
metabolites.
Microalgae-based
have
mostly
consumed
supplements,
which
are
characterized
by
some
general
uncertainty
with
regards
security
sourced
non-European
countries.
novel
foods
regulation
landmark
Europe’s
law
defining
conditions
new
type
be
commercialized.
Currently,
simplified
centralized
version
place,
around
eleven
microalgae-based
market;
however,
than
half
represented
Schizochytrium
sp.
derived
(DHA-rich
oil).
Microalgae
immense
source;
nonetheless,
there
limited
experience
assessing
safety
these
microorganisms,
considering
undesirable
substances
present
way
they
produced
diverse
Here,
we
overview
regulatory
focus
market
introduction,
highlighting
administrative
procedures
scientific
requirements
assess
safety.
We
also
discuss
implications
Transparency
related
considerations
solid
interaction
between
academia
industry.
Frontiers in Microbiology,
Journal Year:
2024,
Volume and Issue:
15
Published: May 6, 2024
Bifidobacteria
are
a
prominent
type
of
bacteria
that
have
garnered
significant
research
attention
for
their
exceptional
probiotic
properties
and
capacity
to
produce
exopolysaccharides
(EPSs).
These
compounds
exhibit
diverse
physical,
chemical,
biological
characteristics,
prompting
numerous
investigations
into
potential
applications.
Researchers
noted
beneficial
effects
as
immune
modulators
within
the
host’s
body
across
various
industries.
Extensive
has
been
conducted
on
immunomodulatory
bifidobacteria-derived
EPSs,
with
emerging
engineering
strategies
aimed
at
enhancing
immune-modulating
capabilities.
Understanding
structure,
physicochemical
properties,
activities
these
is
crucial
effective
utilization
different
Our
review
encompassed
studies
exploring
Bifidobacterium
its
metabolites,
including
sectors,
drawing
from
databases.
The
distinctive
EPSs
spurred
applications,
revealing
bolster
system,
combat
inflammation,
treat
ailments.
Additionally,
possess
antioxidant
antimicrobial
making
them
suitable
incorporation
range
products
spanning
food,
health,
medicine.
EFSA Journal,
Journal Year:
2022,
Volume and Issue:
20(7)
Published: July 1, 2022
The
qualified
presumption
of
safety
(QPS)
approach
was
developed
to
provide
a
regularly
updated
generic
pre-evaluation
the
microorganisms,
intended
for
use
in
food
or
feed
chains,
support
work
EFSA's
Scientific
Panels.
QPS
is
based
on
an
assessment
published
data
each
agent,
with
respect
its
taxonomic
identity,
body
relevant
knowledge,
concerns
and
occurrence
antimicrobial
resistance.
Safety
identified
unit
(TU)
are,
where
possible,
confirmed
at
species/strain
product
level
reflected
by
'qualifications'.
In
period
covered
this
statement,
no
new
information
found
that
would
change
status
previously
recommended
TUs.
Of
50
microorganisms
notified
EFSA
October
2021
March
2022
(inclusive),
41
were
not
evaluated:
10
filamentous
fungi,
1
Microorganisms,
Journal Year:
2023,
Volume and Issue:
11(1), P. 140 - 140
Published: Jan. 5, 2023
Lacticaseibacillus
rhamnosus
is
a
generalist
that
can
adapt
to
different
ecological
niches,
serving
as
valuable
source
of
probiotics.
The
genome
L.
X253
contains
one
chromosome
and
no
plasmids,
with
size
2.99
Mb.
Both
single-copy
orthologous
gene-based
phylogenetic
analysis
average
nucleotide
identity
indicated
dairy-derived
was
most
closely
related
the
human-intestine-derived
strain
LOCK908,
rather
than
other
dairy
strains.
adaptation
GG
niches
explained
by
structural
variation
COG
annotation.
Hemolytic
assays,
API
ZYM
antimicrobial
susceptibility
tests
were
performed
validate
risk-related
sequences
such
virulence
factors,
toxin-encoding
genes,
antibiotic-resistance
genes
in
genomes
GG.
results
showed
able
use
L-fucose,
had
higher
tolerance
bile
salt,
adhered
better
CaCo-2
cells.
In
contrast,
capable
utilizing
D-lactose,
withstood
larger
quantities
hydrogen
peroxide,
possessed
excellent
antioxidant
properties.
This
study
confirmed
safety
probiotic
properties
via
complete
phenotype
analysis,
suggesting
its
potential
probiotic.
Fermentation,
Journal Year:
2022,
Volume and Issue:
8(8), P. 407 - 407
Published: Aug. 19, 2022
Probiotic
milk
is
a
class
of
fermented
that
possesses
health-promoting
effects,
not
only
due
to
the
lactic
acid
bacteria
(LAB)
presence
but
potentially
also
yeast
activity.
Hence,
aim
this
work
was
isolate
and
select
yeasts
from
spontaneous
fermentations
be
used
as
inoculum,
together
with
LAB,
for
manufacturing
probiotic
acidic
low-alcohol
milk.
Six
species
were
detected
fermentation.
A
screening
13
strains
14
previously
isolated
LAB
strains,
based
on
resistance
bile
salts
conditions,
carried
out.
The
best
performing
successively
tested
in
vitro
gastrointestinal
tolerance.
strain
Kluyveromyces
marxianus
Lactococcus
lactis
selected
two
different
values
main
technological
microbiological
parameters
(pH,
organic
acids,
ethanol,
microbial
concentrations)
experimental
range
those
reported
category
products.
evaluation
microorganism
survival
samples
subjected
simulated
conditions
highlighted
high
both
strains.
In
conclusion,
starter
culture
enabled
setting
up
potential
Biotechnology for Biofuels and Bioproducts,
Journal Year:
2022,
Volume and Issue:
15(1)
Published: Dec. 29, 2022
Yeast
was
the
first
microorganism
used
by
mankind
for
biotransformation
processes
that
laid
foundations
of
industrial
biotechnology.
In
last
decade,
Pichia
pastoris
has
become
leading
eukaryotic
host
organism
bioproduct
generation.
Most
P.
bioprocess
operations
been
relying
on
toxic
methanol
and
glucose
feed.
actual
bioeconomy
era,
sustainable
value-added
generation,
non-conventional
yeast
should
be
extended
to
low-cost
renewable
substrates
large
volume
bio-based
commodity
productions.
this
review,
we
evaluated
potential
establishment
circular
due
its
generate
industrially
relevant
bioproducts
from
sources
waste
streams
in
a
cost-effective
environmentally
friendly
manner.
Furthermore,
discussed
challenges
with
second
generation
platforms
propose
novel
insights
future
perspectives.
regard,
low
cost
substrate
candidates,
i.e.,
lignocellulosic
biomass
components,
cereal
by-products,
sugar
industry
by-products
molasses
sugarcane
bagasse,
high
fructose
syrup
biodiesel
by-product
crude
glycerol,
kitchen
other
agri-food
products
were
cell
growth
promoting
effects
recombinant
protein
production.
Further
metabolic
pathway
engineering
construct
utilization
pathways
discussed.
Although,
valorisation
wastes
still
infancy,
rapidly
emerging
synthetic
biology
tools
will
pave
way
more
environment
bioeconomy.
From
environmental
point
view,
development
is
also
important
recycling
otherwise
disposal
carbon-rich
effluents
creates
concerns.
tolerance
contaminants
found
hydrolysate
effluent
glycerol
provides
advantages
extend
applications
toward
strain
design
engineering,
years
come.
Current Issues in Molecular Biology,
Journal Year:
2022,
Volume and Issue:
44(5), P. 2321 - 2334
Published: May 19, 2022
In
recent
years,
alongside
the
conventional
screening
procedures
for
evaluation
of
probiotics
human
usage,
pharmaceutical
and
food
industries
have
encouraged
scientific
research
towards
selection
new
probiotic
bacterial
strains
with
particular
functional
features.
Therefore,
this
study
intended
to
explore
novel
properties
five
Lactiplantibacillus
plantarum
isolated
from
bee
bread.
Specifically,
antioxidant,
antimicrobial
β-glucosidase
activities,
exopolysaccharides
(EPS)
production
ability
synthesize
γ-aminobutyric
acid
(GABA)
were
evaluated.
The
results
demonstrated
that
investigated
L.
effective
in
inhibiting
growth
some
opportunistic
pathogens
vitro
(Pseudomonas
aeruginosa,
Escherichia
coli,
Proteus
mirabilis,
Enterococcus
faecalis
Staphylococcus
aureus).
Moreover,
antioxidant
activity
EPS
GABA
production,
revealed
a
different
behavior
among
strains,
testifying
how
these
are
strongly
strain-dependent.
This
suggests
careful
within
given
species
is
important
order
identify
appropriate
specific
biotechnological
applications.
highlighted
promising
candidates
application
as
dietary
supplements
diet
microbial
cultures
productions.
