Extraction recovery and speciation of selenium in Se-enriched yeast DOI

Ange Angaïts,

Katarzyna Bierła, Joanna Szpunar

et al.

Analytical and Bioanalytical Chemistry, Journal Year: 2024, Volume and Issue: 416(23), P. 5111 - 5120

Published: July 30, 2024

Language: Английский

Development of CRISPR/Cas9-Mediated Saccharomyces cerevisiae Strains for the Cell-Surface Display of a Novel Fusion Acid-Alkaline Phytase DOI

Luc Mai Thanh,

Vo Thi Hoang Lan,

Chau Quoc Cuong

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Phytases enhance phosphorus bioavailability in animal feed, but their limited reusability hinders application. To overcome this, Saccharomyces cerevisiae was engineered to display a fusion phytase combining acid and alkaline phytases on its cell surface by using CRISPR/Cas9. The enzyme anchored via the α-agglutinin-GPI system two marker-free strains, BY4743::GAP-mGEB BY4743::GAP-aGEB, employing MFα Aga2p signal peptides, respectively. Both strains exhibited robust activity across broad pH range, retaining >50% relative between 1.0 7.0, with dual optima at 2.0 5.0–6.0. Kinetic analysis revealed Km of 0.377–0.989 mM kcat 0.014–0.019 μmol/min/mg wet-cell weight, strain showing highest efficiency. ∼ 3.5–4 times higher than single phytase. These effectively degraded phytate soybean, corn flour, rice bran, demonstrating sustainable approach for improving utilization feed.

Language: Английский

Citations

1
Safety and efficacy of a feed additive consisting of inactivated selenised yeast (Saccharomyces cerevisiae CCTCC M 2022402) for all animal species (Phytobiotics Futterzusatzstoffe GmbH) DOI Creative Commons
Vasileios Bampidis,

Giovanna Azimonti,

Maria de Lourdes Bastos

et al.

EFSA Journal, Journal Year: 2024, Volume and Issue: 22(4)

Published: April 1, 2024

Following a request from the European Commission, EFSA was asked to deliver scientific opinion on safety and efficacy of selenised yeast (inactivated)

Language: Английский

Citations

5
Consumer safety of feed additives containing selenium DOI Creative Commons
Vasileios Bampidis,

Giovanna Azimonti,

Maria de Lourdes Bastos

et al.

EFSA Journal, Journal Year: 2024, Volume and Issue: 22(6)

Published: June 1, 2024

Abstract Following a request from the European Commission, EFSA was asked to deliver scientific opinion on safety for consumer of products animals fed diets with feed additives containing selenium as an active substance. Based limited data set available and several uncertainties, FEEDAP Panel concluded that use organic at currently maximum authorised level 0.2 mg supplemented sources/kg complete (within 0.5 total selenium/kg feed) leads exceedance UL all population categories (except elderly very elderly), suggesting concern safety. It not possible conclude categories. Additional studies specifically designed measure deposition in tissues animal origin resulting different sources would be required perform proper risk assessment.

Language: Английский

Citations

4
Use of pigmented fungi as additives in aquaculture DOI Creative Commons

Christina Zantioti,

Arkadios Dimitroglou,

Konstantinos C. Mountzouris

et al.

Aquaculture International, Journal Year: 2025, Volume and Issue: 33(3)

Published: Jan. 28, 2025

Language: Английский

Citations

0
Extraction recovery and speciation of selenium in Se-enriched yeast DOI

Ange Angaïts,

Katarzyna Bierła, Joanna Szpunar

et al.

Analytical and Bioanalytical Chemistry, Journal Year: 2024, Volume and Issue: 416(23), P. 5111 - 5120

Published: July 30, 2024

Language: Английский

Citations

1