LncRNA GAS5 restrains ISO-induced cardiac fibrosis by targeting miR-217 through regulating SIRT1 DOI Creative Commons
Yanhong Zhang,

Tingting Sun,

Zhenhuan Liu

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: May 18, 2023

Abstract Aims : Considering the SIRT1 has effect of improving myocardial fibrosis and GAS5 can inhibit occurrence development at cellular level, aim present study was to investigate whether LncRNA could attenuate cardiac through regulating miR-217/SIRT1, NLRP3 inflammasome activation involved in this process. Methods Results: Isoprenaline (ISO) given subcutaneously male C57BL/6 mice induce AAV9 vectors were randomly injected into left ventricle each mouse overexpress long-chain non-coding RNA GAS5. Primary fibroblasts (MCFs) derived from neonatal TGF-β1 used fibrosis. And overexpressed MCFs treated with miR-217 mimics inhibitor respectively. Then assays expression levels NLRP3, Caspase-1, IL-1βand conducted. The Results showed that overexpression reduced collagen, Capase-1, IL-1β ISO MCFs. However, significantly weakened after overexpression, but further enhanced knockdown miR-217. Conclusion: down-regulats SIRT1, then aggravates activation-mediated pyroptosis. alleviates induced by pyroptosis via miR-217/SIRT1 pathway. This provides significant experimental evidence suggesting be a potential therapeutic target for other fibrotic diseases.

Language: Английский

Atrazine exposure promotes cardiomyocyte pyroptosis to exacerbate cardiotoxicity by activating NF-κB pathway DOI

Xuyan Zhao,

Huan Luo,

Sai Yao

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 915, P. 170028 - 170028

Published: Jan. 14, 2024

Language: Английский

Citations

13
Programmed death of cardiomyocytes in cardiovascular disease and new therapeutic approaches DOI Creative Commons
Kexin Cai,

Haoyue Jiang,

Yuanming Zou

et al.

Pharmacological Research, Journal Year: 2024, Volume and Issue: 206, P. 107281 - 107281

Published: June 26, 2024

Cardiovascular diseases (CVDs) have a complex pathogenesis and pose major threat to human health. Cardiomyocytes low regenerative capacity, their death is key factor in the morbidity mortality of many CVDs. Cardiomyocyte can be regulated by specific signaling pathways known as programmed cell (PCD), including apoptosis, necroptosis, autophagy, pyroptosis, ferroptosis, etc. Abnormalities PCD lead development variety cardiovascular diseases, there are also molecular-level interconnections between different under same disease model. Currently, link cardiomyocytes not fully understood. This review describes molecular mechanisms impact cardiomyocyte on development. Emphasis placed summary drugs potential therapeutic approaches that used treat targeting blocking cardiomyocytes.

Language: Английский

Citations

9
Integrative transcriptomics and proteomics analysis reveal the protection of Astragaloside IV against myocardial fibrosis by regulating senescence DOI Creative Commons
Lipeng Shi, Jingwei Deng, Jun He

et al.

European Journal of Pharmacology, Journal Year: 2024, Volume and Issue: 975, P. 176632 - 176632

Published: May 6, 2024

Myocardial fibrosis (MF) is a pivotal pathological process implicated in various cardiovascular diseases, particularly heart failure. Astragaloside IV (AS-IV), natural compound derived from Astragalus membranaceus, possesses potent cardioprotective properties. However, the precise molecular mechanisms underlying its anti-MF effects, relation to senescence, remain elusive. Thus, this study aimed investigate therapeutic potential and of AS-IV treating ISO-induced MF mice, employing transcriptomics, proteomics, vitro, vivo experiments. We assessed positive effects on using HE staining, Masson ELISA, immunohistochemical transthoracic echocardiography, transmission electron microscopy, DHE fluorescence staining. Additionally, we elucidated regulatory role through comprehensive transcriptomics proteomics analyses, complemented by Western blotting RT-qPCR validation pertinent pathways. Our findings demonstrated that treatment markedly attenuated myocardial injury oxidative stress, concomitantly inhibiting release SASPs. Furthermore, integrated analyses revealed mechanism was associated with regulating cellular senescence p53 signaling pathway. These results highlight exerts not only stress but also modulating

Language: Английский

Citations

6
Ferroptosis: A novel therapeutic target of natural products against doxorubicin-induced cardiotoxicity DOI Open Access
Xiaojiao Yi, Qi Wang,

Mengjie Zhang

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 178, P. 117217 - 117217

Published: July 29, 2024

Doxorubicin (DOX), a commonly used chemotherapy drug, is hindered due to its tendency induce cardiotoxicity (DIC). Ferroptosis, novel mode of programmed cell death, has received substantial attention for involvement in DIC. Recently, natural product-derived ferroptosis regulator emerged as potential strategy treating In this review, comprehensive search was conducted across PubMed, Web Science, Google Scholar, and ScienceDirect databases gather relevant articles on the use products DIC relation ferroptosis. The available papers were carefully reviewed summarize therapeutic effects underlying mechanisms modulating treatment. It found that plays an important role pathogenesis, with dysregulated expression ferroptosis-related proteins strongly implicated condition. Natural products, such flavonoids, polyphenols, terpenoids, quinones can act GPX4 activators, Nrf2 agonists, lipid peroxidation inhibitors, thereby enhancing viability, attenuating myocardial fibrosis, improving cardiac function, suppressing both vitro vivo models This review demonstrates strong correlation between DOX-induced key proteins, GPX4, Keap1, Nrf2, AMPK, HMOX1. are likely exert against by activity these proteins.

Language: Английский

Citations

6
LncRNA GAS5 restrains ISO-induced cardiac fibrosis by modulating mir-217 regulation of SIRT1 DOI Creative Commons
Yanhong Zhang,

Tingting Sun,

Zhenhua Liu

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: April 1, 2024

Abstract Considering the effect of SIRT1 on improving myocardial fibrosis and GAS5 inhibiting occurrence development at cellular level, aim present study was to investigate whether LncRNA could attenuate cardiac through regulating mir-217/SIRT1, NLRP3 inflammasome activation involved in this process. Isoprenaline (ISO) given subcutaneously male C57BL/6 mice induce AAV9 vectors were randomly injected into left ventricle each mouse overexpress GAS5. Primary fibroblasts (MCFs) derived from neonatal TGF-β1 used fibrosis. And overexpressed MCFs treated with mir-217 mimics inhibitor respectively. Then assays expression levels NLRP3, Caspase-1, IL-1β conducted. The findings indicated that overexpression reduced collagen, Capase-1, ISO MCFs. However, significantly weakened after overexpression, but further enhanced knockdown mir-217. down-regulates SIRT1, leading increased subsequent pyroptosis. alleviates induced via mir-217/SIRT1 pathway.

Language: Английский

Citations

4
Potential of Natural Phenolic Compounds against Doxorubicin-Induced Chemobrain: Biological and Molecular Mechanisms Involved DOI Creative Commons
Simona Serini, Gabriella Calviello

Antioxidants, Journal Year: 2024, Volume and Issue: 13(4), P. 486 - 486

Published: April 18, 2024

Chemotherapy-induced cognitive impairment or "chemobrain" is a prevalent long-term complication of chemotherapy and one the more devastating. Most studies performed so far to identify dysfunctions induced by antineoplastic chemotherapies have been focused on treatment with anthracyclines, frequently administered breast cancer patients, population that, after treatment, shows high possibility long survival and, consequently, chemobrain development. In last few years, different possible strategies explored prevent reduce anthracycline doxorubicin (DOX), known promote oxidative stress inflammation, which strongly implicated in development this brain dysfunction. Here, we critically analyzed results preclinical from years that evaluated potential phenolic compounds (PheCs), large class natural products able exert powerful antioxidant anti-inflammatory activities, inhibiting DOX-induced chemobrain. Several PheCs belonging classes shown be revert morphological damages deficits associated learning, memory, exploratory behavior. We biological molecular mechanisms suggested future perspectives research area.

Language: Английский

Citations

4
Doxorubicin-Induced Cardiac Remodeling: Mechanisms and Mitigation Strategies DOI
Yingpu Sun, Lili Xiao, Linlin Chen

et al.

Cardiovascular Drugs and Therapy, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 26, 2025

Language: Английский

Citations

0
Gegen Qinlian Decoction improves Alzheimer’s disease through TLR4/NF-κB/NLRP3 pathway DOI
Bin Zhang,

Liudan Chen,

Mengru Kang

et al.

Tissue and Cell, Journal Year: 2025, Volume and Issue: 95, P. 102818 - 102818

Published: March 1, 2025

Language: Английский

Citations

0
Quality Study of HuangqiGuizhiWuwu Decoction Based on Ultra Performance Liquid Chromatography Fingerprinting, Chemical Pattern Recognition and Multi‐Component Quantitative Analysis DOI Open Access
Xin‐Yue Hu,

Qi‐Song Chen,

Shun‐Qing He

et al.

Separation Science Plus, Journal Year: 2025, Volume and Issue: 8(3)

Published: Feb. 28, 2025

ABSTRACT HuangqiGuizhiWuwu decoction (HGWD) possesses a complex and diverse chemical composition, rendering the identification of its components challenging. In this study, UPLC fingerprinting profile HGWD was elucidated method determining multiple in proposed. Common peaks were selected from 12 batches HGWD, Chinese herbal chromatographic fingerprint similarity evaluation system (2012) employed for analyses. The corresponding to common identified using UPLC‐Q‐TOF‐MS. Chemical analyses conducted evaluate quality different identify primary components. similarities profiles all > 0.98, with 15 confirmed divided into three categories, OPLS‐DA used cinnamic acid cinnamaldehyde as characteristic that caused differences. five which included albiflorin, paeoniflorin, calycosin‐7‐O‐β‐D‐glucoside, acid, cinnamaldehyde, exhibited good linear relationships average recovery rates relative standard deviations. This study established proposed simple, feasible, sensitive, reproducible content determination method. Moreover, our findings provide robust foundation standardization HGWD.

Language: Английский

Citations

0
From Traditional Efficacy to Drug Design: A Review of Astragali Radix DOI Creative Commons
Xiaojie Jin, Huijuan Zhang,

Xiaorong Xie

et al.

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(3), P. 413 - 413

Published: March 14, 2025

Astragali Radix (AR), a traditional Chinese herbal medicine, is derived from the dried roots of Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao (A. mongholicus, AMM) or Bge membranaceus, AM). According to medicine (TCM) theory, AR believed tonify qi, elevate yang, consolidate body’s surface reduce sweating, promote diuresis and swelling, generate body fluids, nourish blood. It has been widely used treat general weakness chronic illnesses improve overall vitality. Extensive research identified various medicinal properties AR, including anti-tumor, antioxidant, cardiovascular-protective, immunomodulatory, anti-inflammatory, anti-diabetic, neuroprotective effects. With advancements in technology, methods such as computer-aided drug design (CADD) artificial intelligence (AI) are increasingly being applied development TCM. This review summarizes progress on over past decades, providing comprehensive overview its efficacy, botanical characteristics, distribution, chemical constituents, phytochemistry. aims enhance researchers’ understanding pharmaceutical potential, thereby facilitating further utilization.

Language: Английский

Citations

0