Cylindracin, a Cys‐rich protein expressed in the fruiting body of Cyclocybe cylindracea, inhibits growth of filamentous fungi but not yeasts or bacteria DOI Creative Commons
Yamato Kuratani,

Chika Abematsu,

Keisuke Ekino

et al.

FEBS Open Bio, Journal Year: 2024, Volume and Issue: 14(11), P. 1805 - 1824

Published: Oct. 8, 2024

Mushrooms are the fruiting bodies of fungi and important reproductive structures that produce disseminate spores. The Pri3 gene was originally reported to be specifically expressed in primordia (a precursor mature body) edible mushroom Cyclocybe aegerita . Here, we cloned a ‐related cDNA from cylindracea , another species same genus, showed is at pileus surface immature body but not primordia. Immunohistochemistry translated protein secreted into polysaccharide layer surface. recombinant C ‐terminal Cys‐rich domain antifungal activity against three filamentous inhibited hyphal growth conidiogenesis. These results suggest PRI3‐related C. named cylindracin, plays an role defense pathogens.

Language: Английский

NUA4 histone acetylase gene LeEaf6 regulates precocity of shiitake mushroom, Lentinula edodes DOI
Lu Wang, Wenbing Gong,

Haoyu Xie

et al.

Scientia Horticulturae, Journal Year: 2024, Volume and Issue: 328, P. 112912 - 112912

Published: Jan. 25, 2024

Language: Английский

Citations

1
Unraveling Morphogenesis, Starvation, and Light Responses in a Mushroom-Forming Fungus,Coprinopsis cinerea, Using Long Read Sequencing and Extensive Expression Profiling DOI Creative Commons
Botond Hegedüs, Neha Sahu, Balázs Bálint

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 10, 2024

Abstract Mushroom-forming fungi (Agaricomycetes) are emerging as pivotal players in several fields, drivers of nutrient cycling, sources novel applications, and the group includes some most morphologically complex multicellular fungi. Genomic data for Agaricomycetes accumulating at a steady pace, however, this is not paralleled by improvements quality genome sequence associated functional gene annotations, which leaves function notoriously poorly understood comparison with other model eukaryotes. We set out to improve our understanding mushroom Coprinopsis cinerea integrating new, chromosome-level assembly high-quality predictions information derived from gene-expression profiling across 67 developmental, stress, light conditions. The new annotation has considerably improved metrics 5’- 3’-untranslated regions (UTRs), polyadenylation sites (PAS), upstream ORFs (uORFs), splicing isoforms, conserved motifs (e.g., TATA Kozak boxes) microexons. found that alternative widespread C. , but it specifically regulated various conditions used here. Transcriptome allowed us delineate core sets corresponding carbon starvation, light-response, hyphal differentiation, uncover aspects light-regulated phases life cycle. As result, now become comprehensively annotated among mushroom-forming fungi, will contribute multiple rapidly expanding including research on their history, stress responses, well development.

Language: Английский

Citations

1
Navigating the labyrinth – Searching for mechanistic clues to explain the evolution of sequestrate Agaricales with labyrinthine internal chambers DOI Creative Commons
Andy R. Nilsen, Jonathan M. Plett, Tom W. May

et al.

Fungal Biology Reviews, Journal Year: 2024, Volume and Issue: 49, P. 100380 - 100380

Published: July 1, 2024

Fungi exhibit a wide range of sporophore morphologies. Amongst the Agaricomycetes, sporophores include mushroom, coralloid, bracket and sequestrate forms. A striking observation is repeated independent evolution forms, which have arisen more than 100 times from lineages where exposed spore-bearing tissues are ancestral condition. Here we review particular morphology in Agaricales, labyrinthine syndrome. We draw on knowledge genetic mechanisms involved development agarics (mushrooms) suggest potential changes relation to alterations pileus, lamellae stipe during development. discuss that could give rise

Language: Английский

Citations

1
A new regulator of sporulation sheds light on spore morphogenesis and ballistospory in mushroom-forming fungi DOI Creative Commons
Zhihao Hou, Zsolt Merényi, Yashu Yang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 26, 2024

Abstract Sporulation is the most widespread means of reproduction and dispersal in fungi. In Basidiomycota, sexual spores are produced on specialised cells known as basidia, from which they discharged forcibly by a powered process called ballistospory, highest acceleration nature. However, genetics sporulation, particular postmeiotic events related to spore morphogenesis remain poorly known. Here, we explore these processes, based new, highly conserved transcription factor, Sporulation-Related Regulator 1 (SRR1), its putative downstream regulatory network. Reverse Srr1 model mushroom Coprinopsis cinerea commercially oyster indicated role sporulation across Agaricomycetes. RNA-Seq analysis motif-based inference hypothetical SRR1 gene network allowed delimiting targets regulated direct indirect manner. Using this comparative genomics, identified genes associated with including SRR1-target chitinase, was found be required for normal production morphology. Overall, our study offers new insights into genetic mechanisms governing ballistospory

Language: Английский

Citations

1
Cylindracin, a Cys‐rich protein expressed in the fruiting body of Cyclocybe cylindracea, inhibits growth of filamentous fungi but not yeasts or bacteria DOI Creative Commons
Yamato Kuratani,

Chika Abematsu,

Keisuke Ekino

et al.

FEBS Open Bio, Journal Year: 2024, Volume and Issue: 14(11), P. 1805 - 1824

Published: Oct. 8, 2024

Mushrooms are the fruiting bodies of fungi and important reproductive structures that produce disseminate spores. The Pri3 gene was originally reported to be specifically expressed in primordia (a precursor mature body) edible mushroom Cyclocybe aegerita . Here, we cloned a ‐related cDNA from cylindracea , another species same genus, showed is at pileus surface immature body but not primordia. Immunohistochemistry translated protein secreted into polysaccharide layer surface. recombinant C ‐terminal Cys‐rich domain antifungal activity against three filamentous inhibited hyphal growth conidiogenesis. These results suggest PRI3‐related C. named cylindracin, plays an role defense pathogens.

Language: Английский

Citations

1