Transient expression of fluorescent proteins and Cas nucleases in Phytophthora agathidicida via PEG-mediated protoplast transformation

Microbiology, Journal Year: 2025, Volume and Issue: 171(3)

Published: March 28, 2025

Phytophthora species are eukaryotic plant pathogens that cause root rot and dieback diseases in thousands of worldwide. Despite their significant economic ecological impacts, fundamental molecular tools such as DNA transformation methods not yet established for many species. In this study, we have a PEG/calcium chloride (CaCl 2 )-mediated protoplast method agathidicida , the causal agent kauri disease. Adapting protocol from sojae systematically optimized digesting enzymes, recovery media composition pH. Our findings reveal chitinases essential P. formation, optimum pH medium is 5. The type did significantly impact regeneration. Using protocol, generated transformants using three plasmids (i.e. pTdTomatoN, pYF2-PsNLS-Cas9-GFP pYF2-PsNLS-Cas12a-GFP), which expressed fluorescent proteins and/or Cas nucleases. were unstable unless maintained under antibiotic selective pressure; however, selection, fluorescence was across multiple generations life cycle stages, including production zoospores transformed mycelia. Notably, observed expression GFP-tagged nucleases, promising future CRISPR-Cas genome editing applications. This study demonstrates amenable to PEG/CaCl -mediated transformation. Although resulting require pressure remain stable, transient system can be valuable applications cell tracking, chemotaxis studies editing. also provides foundation further optimization methods. It serves tool exploring biology potentially other closely related

Language: Английский

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Rediscovery of Araiospora Thaxter: new insights into a neglected chapter of oomycete evolution

Mycological Progress, Journal Year: 2025, Volume and Issue: 24(1)

Published: March 27, 2025

Language: Английский

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Phytophthora inundata: A New Root Pathogen of Citrus in Europe and the Mediterranean Region

Plants, Journal Year: 2025, Volume and Issue: 14(9), P. 1333 - 1333

Published: April 28, 2025

Citruses are one of the major fruit crops globally. Among Mediterranean citrus producers, Sicily (southern Italy) is renowned for its high-quality fresh production. Phytophthora diseases a serious issue production worldwide and nicotianae prevalent causal agent root rot in most growing areas globally particularly region. This study reports occurrence inundata as pathogen declining mature trees eastern association with P. nicotianae. first record on Europe The species was identified basis morphology multi-gene phylogenetic analysis, which included internal transcribed spacer, β-tubulin cytochrome c oxidase subunit 1. Pathogenicity tests saplings showed less aggressive than However, co-inoculation both produced more severe symptoms inoculation single species, indicating an additive effect these two pathogens suggesting that opportunistic secondary like may have crucial role complex diseases.

Language: Английский

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Microbiome-Mediated Disease Suppression: A Promising Avenue for Phytophthora Management in Commercial Crops Using Phytochemicals Obtained from Medicinal Plants

Industrial Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: April 30, 2025

Language: Английский

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First report of the occurrence of Phytophthora honggalleglyana in an urban green space in Poland

Urban forestry & urban greening, Journal Year: 2025, Volume and Issue: unknown, P. 128834 - 128834

Published: May 1, 2025

Language: Английский

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Oomycete communities are influenced by land use and disease status in Christmas tree production in Southern Québec, Canada

Environmental DNA, Journal Year: 2024, Volume and Issue: 6(2)

Published: March 1, 2024

Abstract Forests are threatened by many natural stressors intensified climate change and anthropogenic activities, which tend to increase their susceptibility pests pathogens. Consequently, oomycete‐related forest decline or dieback cases increasing in natural, urban, agricultural landscapes. It is this context that Christmas tree growers from Southern Québec, Canada, experiencing root rot problems, with reported incidences up 25%. In a previous study, seven Phytophthora spp. were associated problem, but the overall diversity of oomycetes has not yet been investigated. Hence, we use metabarcoding approach provide an overview diversity, richness, composition oomycete community fir plantations compared surrounding forests. We showed P. cryptogea cluster, europaea sansomeana , chlamydospora cluster significantly more abundant soils collected under diseased trees confirmed (including abietivora ) was most frequently showing rot‐like symptoms. Finally, report land (anthropogenic activities) shapes while can act as gateway for invading fact, results presented here suggested might already have crossed boundary other species follow, advocating importance improved surveillance various environments.

Language: Английский

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Response of Alnus glutinosa to Phytophthora bark infections at ambient and elevated CO2 levels

Frontiers in Forests and Global Change, Journal Year: 2024, Volume and Issue: 7

Published: April 17, 2024

Introduction Mortality of the riparian alder population caused by Phytophthora pathogens has been studied for over 20 years throughout Europe, recently gaining more importance in context evident climate change. The main objective this study was to examine pathogenicity species from “ alni complex ” present Czech Republic ( P . × and P. uniformis ) plurivora Alnus glutinosa seedlings grown at ambient elevated CO 2 concentration. Methods An underbark inoculation test performed with seeds collected two populations, one suffering severe decline other disease-free. Results results showed significant differences lesion development seedling mortality. After a 13-week experimental period, both levels high aggressiveness A. causing lesions variable sizes mortality 33.3%, 45.8% plants, respectively. In contrast, did not cause any plant, differ significantly those control plants. Physiological measurements reveal between except plants inoculated showing increased values specific physiological parameters 4 weeks post-inoculation. Net photosynthesis decreased measurement period all treatments found conducted after inoculation. Transpiration decreasing trend no levels. Chemical analyses root samples variability sugars phenolic compounds related plant's health status. Discussion This is first response different findings demonstrate weaker regardless level.

Language: Английский

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Impacts of phosphite treatment on Phytophthora community assemblages and inoculum abundances in Phytophthora-infected forest soil

Trees Forests and People, Journal Year: 2024, Volume and Issue: unknown, P. 100687 - 100687

Published: Sept. 1, 2024

Language: Английский

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A Systematic Review of Alternative Host Species of Phytophthora erythroseptica, the Major Causal Agent of Pink Rot of Potato

Plant Pathology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

ABSTRACT Most published studies of Phytophthora erythroseptica , the major causal agent pink rot potato, have focused on fungicide disease management, particularly in light detection fungicide‐sensitive isolates cropping systems. In addition to use, crop rotation practices are also identified as important minimise risk disease; however, due broad spectrum plants putatively infected by P . including both solanaceous and non‐solanaceous species, choice effective crops can be problematic. To make informed decisions about systems, an unambiguous understanding alternative host status importance these hosts inoculum maintenance is required. The aim this systematic review analyse current knowledge natural field environments. Searches records from 1913 2023 obtained 44 articles relevant pathogen covering infection artificial inoculation experiments. Complexity morphological descriptions has raised questions over evidence for certain records. Additional molecular provided greater certainty record authenticity provides appropriate standard which confirm status. Given increasingly limited cultural chemical management alternatives, control future will rely heavily rotation, implementing careful selection pasture species targeted weed remove hosts, preventing proliferation soil build‐up. Thus, it critical that data accurate.

Language: Английский

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A pangenome analysis reveals the center of origin and evolutionary history of Phytophthora infestans and 1c clade species

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(1), P. e0314509 - e0314509

Published: Jan. 24, 2025

We examined the evolutionary history of Phytophthora infestans and its close relatives in 1c clade. used whole genome sequence data from 69 isolates species clade conducted a range genomic analyses including nucleotide diversity evaluation, maximum likelihood trees, network assessment, time to most recent common ancestor migration analysis. consistently identified distinct later divergence two Mexican species, P . mirabilis ipomoeae , other species. exhibited more South America, andina betacei Speciation evolution occurred Andes. andina–P betacei–P formed complex with indistinct boundaries, hybridizations between short times ancestry. Furthermore, distinction modern American proved less discrete, suggesting gene flow populations over time. Admixture analysis indicated relationship among these populations, hinting at potential across regions. Historic collected 1845–1889, were first diverge all populations. Modern diverged next followed by which showed Both derived historic Based on closest relatives, Andean region, we consider Andes be center origin globalization contributing admixture today Mexico, Europe.

Language: Английский

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