Biotechnological potential of salt tolerant and xerophilic species of Aspergillus DOI Creative Commons
István Pócsi, Jan Dijksterhuis, Jos Houbraken

et al.

Applied Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 108(1)

Published: Nov. 19, 2024

Abstract Xerophilic fungi occupy versatile environments owing to their rich arsenal helping them successfully adapt water constraints as a result of low relative humidity, high-osmolarity, and high-salinity conditions. The general term xerophilic relates organisms that tolerate and/or require reduced activity, while halophilic osmophilic are applied specialized groups high salt concentrations or increased osmotic pressure, respectively. Species belonging the family Aspergillaceae , especially those classified in Aspergillus subgenus (sections Restricti ) Polypaecilum particularly enriched group salt-tolerant filamentous fungi. They produce an unprecedently wide spectrum tolerant enzymes including proteases, peptidases, glutaminases, γ-glutamyl transpeptidases, various glycosidases such cellulose-decomposing starch-degrading hydrolases, lipases, tannases, oxidareductases. These extremophilic also represent huge untapped treasure chest yet-to-be-discovered, highly valuable, biologically active secondary metabolites. Furthermore, these indispensable agents decolorizing textile dyes, degrading xenobiotics removing excess ions high-salt environments. could play role fermentation processes at activity leading preparation daqu, meju, tea. Considering current future agricultural applications, Aspergilli may contribute biosolubilization phosphate soil amelioration stress crops. Transgenes from halophile find promising applications engineering drought-tolerant spoil feed food raise mycotoxin above permissible doses and, therefore, development novel preservation technologies against spp. is urgently needed. On other hand, some have been shown be biological control mites. Key points • Salt can found resources valuable metabolites Biotechnological expanding

Language: Английский

What are the 100 most cited fungal genera? DOI

C.S. Bhunjun,

Yijun Chen, Chayanard Phukhamsakda

et al.

Studies in Mycology, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

Citations

23
Phylogenomics reveals extensive misidentification of fungal strains from the genus Aspergillus DOI Creative Commons
Jacob L. Steenwyk,

Charu Balamurugan,

Huzefa A. Raja

et al.

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: 12(4)

Published: March 6, 2024

Modern taxonomic classification is often based on phylogenetic analyses of a few molecular markers, although single-gene studies are still common. Here, we leverage genome-scale phylogenetics (phylogenomics) species and populations to reconstruct evolutionary relationships in dense data set 710 fungal genomes from the biomedically technologically important genus Aspergillus. To do so, generated novel 1,362 high-quality markers specific for Aspergillus provided profile Hidden Markov Models each, facilitating their use by others. Examining resulting phylogeny helped resolve ongoing controversies, identified new ones, revealed extensive strain misidentification (7.59% strains were previously misidentified), underscoring importance population-level sampling classification. These findings corroborated using current standard, taxonomically informative loci. suggest that phylogenomics can facilitate accurate classifications reconstructions Tree Life.IMPORTANCEIdentification relies markers. Advances genomic technologies have made it possible sequence genome any strain, making assignment (and discovery ones). We examined usefulness limitations large publicly available multiple biomedically, agriculturally, industrially Our nearly 8% misidentified. work highlights systematic biology suggests sequencing strains, including reference (e.g., type strains), will be required reduce errors public databases.

Language: Английский

Citations

14
Penicillium psychrofluorescens sp. nov., a naturally autofluorescent Antarctic fungus DOI Creative Commons
Alinta Furnell, Nicole Benaud, Xabier Vázquez-Campos

et al.

Mycology&#58 An International Journal on Fungal Biology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 24

Published: Jan. 2, 2025

A new fungal species, Penicillium psychrofluorescens sp. nov. is described as a member of section Torulomyces. The species sister to P. catalonicum, and was isolated from soil collected Robinson Ridge, East Antarctica, following enrichment cultivation under oligotrophic conditions supplemented with excess hydrogen gas. named for its intense autofluorescence derived combination compounds that may include NADPH, porphyrins, secondary metabolites, such polyketides. Comparative genomics both Antarctic mesophilic spp. shows has wide repertoire glycoside hydrolases, but almost no polysaccharide lyases, comparably large effector proteins, lacks the machinery use nitrate an N-source, genes assimilation phosphorus phosphonates via oxidative pathway. strain found have 30 biosynthetic gene clusters (BGCs), majority which were unrelated known compound BGCs. Given remarkable diversity natural products already characterised presence >30 BGCs low similarity genes, there biotechnological potential within this novel yet be explored.

Language: Английский

Citations

0
Inside out: New root endophytic Penicillium and Talaromyces species isolated from Cattleya orchids (Orchidaceae) in Brazil DOI
Thiago Oliveira Condé, Danilo Oliveira Ramos,

Pedro Tiago Santos Nogueira

et al.

Fungal Systematics and Evolution, Journal Year: 2025, Volume and Issue: 15(1), P. 179 - 200

Published: Jan. 30, 2025

The Orchidaceae family comprises a highly diverse group of flowering plants. genus Cattleya is restricted to the Neotropics, with approximately 80 % species present in Brazil occurring epiphytic, rupicolous, and terrestrial habitats. During surveys that aimed investigate root fungal endophytes two native orchids, C. locatellii violacea, total 10 endophytic isolates were identified as belonging Eurotiales. A polyphasic approach was applied for identification characterization cultured species, combining morphological molecular data. Phylogenetic analyses performed using internal transcribed spacers (ITS) rDNA, beta-tubulin (BenA), calmodulin (CaM), RNA polymerase second-largest subunit (RPB2) sequences. Two new described from roots locatellii, namely Penicillium endophyticum sp. nov. (section Aspergilloides), Talaromyces cattleyae Purpurei). In addition, P. yuyongnianii Lanata-Divaricata), T. amestolkiae, atkinsoniae Talaromyces) reported Cattleya. Citation: Condé TO, Ramos DO, Nogueira PTS, Pereira OL (2025). Inside out: New isolated orchids (Orchidaceae) Brazil. Fungal Systematics Evolution 15: 179-200. doi: 10.3114/fuse.2025.15.08.

Language: Английский

Citations

0
Cyclodepsipeptides and Fatty Acid Lactones from the Freshwater-Derived Fungus, Talaromyces gwangjuensis, and Their Potential as Autophagy Activators DOI
Hieu Van, Seri Choi, Jorge-Eduardo Ponce-Zea

et al.

Journal of Natural Products, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

Autophagy is a primary cellular mechanism that entails the degradation and recycling of impaired or redundant constituents. It plays an essential role in maintaining health homeostasis. Dysfunction autophagy has been implicated wide range diseases, including cancer, cardiovascular neurodegenerative diseases. A total 200 fungal extracts were screened for their ability to modulate HEK293A cells, human kidney cell line stably expressing GFP-tagged LC3, marker autophagy. potential regulator extract was identified from freshwater-derived fungus, Talaromyces gwangjuensis. Through implementation Feature-Based Molecular Networking (FBMN), seven cyclodepsipeptides (1-7) four lactone derivatives (8-11) isolated bioactive fractions. The chemical structure newly compounds, arthrichitins E-H (1-4) gwangjupones A-D (8-11), elucidated using 1D 2D NMR spectroscopy, Marfey's analysis, J-based configuration ECD, DP4+ probability calculations. Compounds 1, 4, 6 found stimulate autophagic flux IMR90 cells infected with adeno-associated virus carrying mCherry-GFP-LC3 construct, highlighting as activators.

Language: Английский

Citations

0
Isolation and Characterization of Four Unreported Penicillium Species Isolated from the Freshwater Environments in Korea DOI Creative Commons
Mingyu Kim,

Seong-Keun Lim,

Chang-Gi Back

et al.

Mycobiology, Journal Year: 2025, Volume and Issue: 53(3), P. 269 - 279

Published: March 14, 2025

The fungal species of the genus Penicillium can be found across a diverse array environments. infrageneric classification has been studied with comparison morphological and phylogenetical features, derived into two subgenus, 32 sections, 89 series. In this study, 11 strains were isolated from freshwater environments, plant litter, nearby substrates in Korea identified as previously unreported species. internal transcribed spacer (ITS) regions, β-tubulin (BenA), calmodulin (CaM), RNA polymerase II subunit (RPB2) genes analyzed for phylogenetic analyses. A neighbor-joining tree was then constructed using concatenated DNA sequences, compared closely related Penicillium. strain clustered distinct lineages, confirming their P. contaminatum, jinfoshanicum, xuanhanense, soppii. NNIBRFG40229 exhibits monoverticillate conidiophores flask-shaped phialides, characteristic contaminatum; NNIBRFG1595 presents divaricate conidiophores, consistent jinfoshanicum; NNIBRFG5602 shows velutinous texture orange pigmentation, resembling xuanhanense; NNIBRFG4602 biverticillate cylindrical metulae, corresponding to This study provides first report these Korea, enhancing taxonomic understanding.

Language: Английский

Citations

0
Three New Species of Penicillium from East and Northeast China DOI Creative Commons
H. Q. Song,

Yi-Jing Ding,

Wen-Ying Zhuang

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(5), P. 342 - 342

Published: May 8, 2024

Penicillium species are ubiquitous in the environment and of substantial importance, especially industrial medical aspects. During our investigation biodiversity Penicillium, three new were discovered soil samples collected from East Northeast China. They determined as to science based on morphological comparisons phylogenetic analyses, found belong subgenus section Robsamsonia Aspergilloides sections Citrina. Descriptions illustrations these provided, their geographic distributions also discussed.

Language: Английский

Citations

3
Five new species of endophytic Penicillium from rubber trees in the Brazilian Amazon DOI
Kaliane Sírio Araújo, Janaina Lana Alves, Olinto Liparini Pereira

et al.

Brazilian Journal of Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 10, 2024

Language: Английский

Citations

3
Deciphering Aspergillus section Terrei in Galleria mellonella model: a clade-specific pathogenicity characterization DOI Creative Commons
Roya Vahedi-Shahandashti, Jos Houbraken, Vít Hubka

et al.

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

ABSTRACT The Aspergillus genus encompasses a diverse array of species, some which are opportunistic pathogens. Traditionally, human aspergillosis has primarily been linked to few predominantly A. fumigatus . Changes in epidemiology and advancements molecular techniques have brought attention less common previously unrecognized pathogenic cryptic species. Despite the taxonomic recognition many species section Terrei , their virulence potential clinical implications, compared terreus sensu stricto remain poorly understood. Hence, current study utilized alternative vivo model Galleria mellonella evaluate 19 accepted classified into three series (major phylogenetic clades): Nivei Ambigui Analyzing median survival rates infected larvae all each revealed that significantly lower Taking closer look at trend within clade, dividing two groups: highly virulent (up 72 h survival) 144 survival). Histological observation, considering fungal distribution filamentation, further supported this assessment, revealing increased hyphal formation Additionally, susceptibility profile conventional antifungals was determined, an azole minimum inhibitory concentration for tested such as niveus iranicus Our results highlight importance identification, they can exhibit different levels show reduced antifungal susceptibility. IMPORTANCE With changing increasingly vulnerable population, emerging Their diversity relevance underexplored, with showing higher virulence, highlighting need better preparedness practice. Using model, we assessed including non-cryptic across significant variation among series, displaying high virulence. analysis confirmed spread more elevated concentrations were also observed certain This presents novel insights pathogenicity emphasizing critical accurately identifying due resistance, may substantial implications.

Language: Английский

Citations

0
Two new species of Penicillium (Eurotiales, Aspergillaceae) from China based on morphological and molecular analyses DOI Creative Commons

Ruina Liang,

Xiang-Hao Lin,

Miaomiao An

et al.

MycoKeys, Journal Year: 2025, Volume and Issue: 116, P. 255 - 274

Published: April 25, 2025

Penicillium is a large and significant genus of fungi, exhibiting widespread distribution across diverse substrates. Ongoing taxonomic nomenclatural revisions have led to an annual increase in the number newly described species. This study two new species, i.e., P.lentum P.tibetense, discovered China. They been identified characterized through morphological examination both single gene multigene phylogenetic analyses. Based on these analyses, was classified within section Brevicompacta, while P.tibetense placed Lanata-Divaricata. Both species exhibited features typical their respective sections. Penicilliumlentum by restricted growth with dense colonies agar media predominantly generates terverticillate conidiophores. Penicilliumtibetense demonstrates rapid has vigorous CYA at 30 °C, producing biverticillate Comprehensive descriptions detailed illustrations were presented. A comparison between closely related taxa provided.

Language: Английский

Citations

0