Reactions Weekly, Journal Year: 2023, Volume and Issue: 1945(1), P. 355 - 355
Published: Feb. 25, 2023
Language: Английский
The Lancet Microbe, Journal Year: 2023, Volume and Issue: 5(1), P. e52 - e61
Published: Dec. 1, 2023
Metagenomic next-generation sequencing (mNGS) allows untargeted identification of a broad range pathogens, including rare or novel microorganisms. Despite the recognition mNGS as valuable diagnostic tool for infections, most relevant indications this innovative strategy remain poorly defined. We aimed to assess determinants positivity and clinical utility mNGS.
Language: Английский
Citations
25
Archives of Virology, Journal Year: 2024, Volume and Issue: 169(9)
Published: Aug. 14, 2024
Language: Английский
Citations
13
Xenotransplantation, Journal Year: 2024, Volume and Issue: 31(2)
Published: March 1, 2024
Abstract Background As sequencing is becoming more broadly available, virus discovery continues. Small DNA viruses contribute to up 60% of the overall load in pigs. Porcine circoviruses (PCVs) are small with a single‐stranded circular genome. They common pig breeds and have not been properly addressed for their potential risk xenotransplantation. Whereas PCV1 non‐pathogenic pigs, PCV2 has associated various disease manifestations. Recently two new described, PCV3 PCV4. While PCV4 currently present mainly Asia, widely distributed, identified commercial wild boars, pigs generated In one case was transmitted by baboons via heart transplantation. pathogenicity controversial initially, however, found be porcine dermatitis nephropathy syndrome (PDNS), reproductive failure, multisystemic inflammation. Inoculation studies infectious clones confirmed that pathogenic. Most importantly, recently discovered human (CV) closely related PCV3. Methods Literature evaluated summarized. A dendrogram existing humans, other animal species created assessed at level. Results We can divided into three species, CV1, CV2, CV3. Human CV2 CV3 closest Conclusions Circoviruses ubiquitous. This communication should create awareness newly circoviruses, which may problem blood transfusions xenotransplantation immune suppressed individuals.
Language: Английский
Citations
4
Emerging infectious diseases, Journal Year: 2024, Volume and Issue: 30(10)
Published: Sept. 25, 2024
We identified a novel human circovirus in an immunocompromised 66-year-old woman with sudden onset of self-limiting hepatitis. detected 1 (HCirV-1) transcripts hepatocytes and the HCirV-1 genome long-term patient's blood, stool, urine. is emerging pathogen that persists susceptible patients.
Language: Английский
Citations
4
Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14
Published: Nov. 15, 2024
Metagenomic next-generation sequencing (mNGS) is a transformative approach in the diagnosis of infectious diseases, utilizing unbiased high-throughput to directly detect and characterize microbial genomes from clinical samples. This review comprehensively outlines fundamental principles, workflow, platforms utilized mNGS technology. The methodological backbone involves shotgun total nucleic acids extracted diverse sample types, enabling simultaneous detection bacteria, viruses, fungi, parasites without prior knowledge agent. Key advantages include its capability identify rare, novel, or unculturable pathogens, providing more comprehensive view communities compared traditional culture-based methods. Despite these strengths, challenges such as data analysis complexity, high cost, need for optimized preparation protocols remain significant hurdles. application across various systemic infections highlights utility. Case studies discussed this illustrate efficacy diagnosing respiratory tract infections, bloodstream central nervous system gastrointestinal others. By rapidly identifying pathogens their genomic characteristics, facilitates timely targeted therapeutic interventions, thereby improving patient outcomes infection control measures. Looking ahead, future disease diagnostics appears promising. Advances bioinformatics tools technologies are anticipated streamline analysis, enhance sensitivity specificity, reduce turnaround times. Integration with decision support systems promises further optimize utilization routine practice. In conclusion, represents paradigm shift field diagnostics, offering unparalleled insights into diversity pathogenesis. While persist, ongoing technological advancements hold immense potential consolidate pivotal tool armamentarium modern medicine, empowering clinicians precise, rapid, pathogen capabilities.
Language: Английский
Citations
4
Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15
Published: Jan. 22, 2025
The family Circoviridae includes the genera Circovirus and Cyclovirus. Cycloviruses have been found in serum samples from chronic HBV, HCV, or HIV-infected individuals as well asymptomatic blood donors. However, research on cycloviruses is relatively limited. We used viral metagenomics to mine, analyze, visualize human virome, successfully identifying three new genomes, each encoding Rep Capsid proteins. These proteins are crucial for replication host-cell interaction: protein involved initiating genome replication, while plays a key role assembly of virions virus's ability interact with host immune systems. Distance matrix phylogenetic analyses show that these share high sequence similarity viruses both humans animals across different regions Africa. This finding not only confirms presence previously uncharacterized blood, but also provides insight into their potential transmission ecological significance. Further needed explore functional roles pathogenesis, particularly how they may influence immunity contribute infections. Additionally, studies investigating range mechanisms cross-species will be essential understanding broader implications animal health.
Language: Английский
Citations
0
Clinical Microbiology Reviews, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 31, 2025
SUMMARY Xenotransplantation, the transplantation of living organs, tissues, or cells between species, carries potential to address global shortage human organs for patients with end-stage organ failure. Recent advances in genetic engineering have improved prospects clinical xenotransplantation by reducing immune and inflammatory responses grafts, controlling coagulation on endothelial surfaces, modifying viral risks, including porcine endogenous retrovirus (PERV). Management infectious risks posed requires meticulous attention biosecure breeding microbiological surveillance source animals recipients consideration novel infection control requirements. Infectious stem from both known pathogens immunosuppressed transplant organisms which manifestations, microbial assays, therapies are generally limited. Both unknown zoonoses may be transmitted pigs humans. Some pig-specific do not infect but systemic manifestations when active within xenograft, cytomegalovirus/porcine roseolovirus (PCMV/PRV), contributes graft rejection consumptive coagulopathy. The role (PERV) humans remains uncertain despite absence documented transmissions availability swine inactivated genomic PERV. New technologies, such as metagenomic sequencing multi-omics approaches, will essential detection infections understanding interactions host’s system, pathogens. These approaches allow development protocols, pathogen requirements, tailored antimicrobial enhance safety success xenotransplantation.
Language: Английский
Citations
0
Neuromethods, Journal Year: 2025, Volume and Issue: unknown, P. 145 - 157
Published: Jan. 1, 2025
Language: Английский
Citations
0
Molecular Phylogenetics and Evolution, Journal Year: 2025, Volume and Issue: 209, P. 108369 - 108369
Published: May 5, 2025
Language: Английский
Citations
0
Immunological Reviews, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 28, 2024
Summary Enteric viruses are the main cause of acute gastroenteritis worldwide with a significant morbidity and mortality, especially among children aged adults. Some enteric also disseminated infections severe neurological manifestations such as poliomyelitis. Protective immunity against these is not well understood in humans, most knowledge coming from animal models, although development poliovirus rotavirus vaccines has extended our knowledge. In classical view, innate involves recognition foreign DNA or RNA by pathogen receptors leading to production interferons other inflammatory cytokines. Antigen uptake presentation T cells B then activate adaptive and, case mucosal immunity, induce secretion dimeric IgA, more potent immunoglobulins viral neutralization. The study Inborn errors (IEIs) offers natural opportunity nonredundant toward pathogens. viruses, patients defective antibodies at risk developing complications. Moreover, recent description low absent antibody protracted associated hepatitis reinforces prominent role control virus.
Language: Английский
Citations
2