Circadian Rhythm, Microglia-Mediated Neuroinflammation, and Alzheimer’s Disease

Neuroscience & Biobehavioral Reviews, Journal Year: 2025, Volume and Issue: 170, P. 106044 - 106044

Published: Feb. 4, 2025

Language: Английский

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Involvement of circadian clock protein PER2 in controlling sleep deprivation induced HMGB1 up-regulation by targeting p300 in the cortex

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 10, 2025

Language: Английский

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Integrative Human Genetic and Cellular Analysis of the Pathophysiological Roles of AnxA2 in Alzheimer’s Disease

Antioxidants, Journal Year: 2024, Volume and Issue: 13(10), P. 1274 - 1274

Published: Oct. 21, 2024

Alzheimer's disease (AD) is an intractable and progressive neurodegenerative disease. Amyloid beta (Aβ) aggregation the hallmark of AD. Aβ induces neurotoxicity through a variety mechanisms, including interacting with membrane receptors to alter downstream signaling, damaging cellular or organelle membranes, interfering protein degradation synthesis, inducing excessive immune-inflammatory response, all which lead neuronal death other pathological changes associated In this study, we extracted gene expression profiles from GSE5281 GSE97760 microarray datasets in GEO (Gene Expression Omnibus) database, as well Human Gene Database. We identified differentially expressed genes brain tissues AD patients healthy persons. Through GO, KEGG, ROC analyses, annexin A2 (AnxA2) was putative target gene. Notably, accumulating evidence suggests that intracellular AnxA2 key regulator various biological processes, endocytosis, transmembrane transport, neuroinflammation, apoptosis. Thus, conducted series cell biology experiments explore function The results indicate knockdown primarily affects oxidative phosphorylation, cycle, AD, processing endoplasmic reticulum, SNARE interactions vesicular autophagy. SH-SY5Y cells secreting Aβ42, exacerbated Aβ42-induced cytotoxicity, death, ROS levels, senescence, altered reduced ATP suggesting its critical role mitochondrial maintenance. also inhibitory effect Aβ42 on migration. overexpression inflammatory response induced by while absence increased pro-inflammatory decreased anti-inflammatory responses. Furthermore, facilitated apoptosis These indicated potential pathophysiological roles

Language: Английский

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Transcriptomic Profiles of AKAP12 Deficiency in Mouse Corpus Callosum

Bioinformatics and Biology Insights, Journal Year: 2024, Volume and Issue: 18

Published: Jan. 1, 2024

A-kinase anchor protein 12 (AKAP12), a scaffold protein, has been implicated in the central nervous system, including blood-brain barrier (BBB) function. Although its expression level corpus callosum is higher than other brain regions, such as cerebral cortex, role of AKAP12 remains unclear. In this study, we investigate impact deficiency by transcriptome analysis using RNA-sequencing (RNA-seq) on knockout (KO) mice. We observed minimal changes, with only 13 genes showing differential expression, Akap12 itself. Notably, Klf2 and Sgk1, potentially involved BBB function, were downregulated KO mice expressed vascular cells similar to Akap12. These changes gene may affect important biological pathways that be associated neurological disorders. Our findings provide an additional data set for future research system.

Language: Английский

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Transcriptomic changes in oligodendrocyte lineage cells during the juvenile to adult transition in the mouse corpus callosum

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Sept. 27, 2024

The corpus callosum, a major white matter tract in the brain, undergoes age-related functional changes. To extend our investigation of gene expression dynamics mouse we compared RNA-seq data from 2 week-old and 12 wild-type C57BL/6 J mice identified differentially expressed genes (e.g., Marcksl1, Chst3, C4b, Neat1, Ndrg1, Emid1, etc.) between these ages. Interestingly, found that highly myelinating oligodendrocytes were upregulated to mice, while oligodendrocyte precursor cells (OPCs) newly formed downregulated. Furthermore, by comparing with datasets 20 96 novel sets age-dependent variations callosum. These changes potentially affect key biological pathways may be closely linked neurological disorders, including dementia stroke. Therefore, results provide an additional dataset explore lineage

Language: Английский

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Sex differences in the role of AKAP12 in behavioral function of middle-aged mice

Biology of Sex Differences, Journal Year: 2024, Volume and Issue: 15(1)

Published: Nov. 21, 2024

Abstract A-kinase anchoring protein 12 (AKAP12) is a key scaffolding that regulates cellular signaling by kinase A (PKA) and other molecules. While recent studies suggest an important role for AKAP12 in the brain, including cognitive functions, its middle-aged mice potential sex differences are not fully understood. Therefore, this study investigated effects of on exploratory behavior mice, focusing differences. Cognitive function was assessed using spontaneous Y-maze test novel object recognition (NORT). No significant were found C57BL/6J mice; however, female showed greater during NORT. In addition, both male Akap12 knockout (KO) performed similarly to wild-type (WT) test, but had lower discrimination indices NORT, suggesting short-term memory. Notably, suppressed KO compared WT whereas did show effect. There no movement distance velocity NORT between either sex. These results indicate affects these differ sexes.

Language: Английский

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Effects of Aging on Diurnal Transcriptome Change in the Mouse Corpus Callosum

iScience, Journal Year: 2024, Volume and Issue: 28(1), P. 111556 - 111556

Published: Dec. 9, 2024

Language: Английский

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