Biological Responses of Pacific Herring Embryos to Crude Oil Are Quantifiable at Exposure Levels Below Conventional Limits of Quantitation for PAHs in Water and Tissues DOI Creative Commons
John P. Incardona,

Tiffany L. Linbo,

James Cameron

et al.

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 57(48), P. 19214 - 19222

Published: Nov. 14, 2023

Pacific herring (Clupea pallasii), a cornerstone of marine food webs, generally spawn on macroalgae in shallow nearshore areas that are disproportionately at risk from oil spills. Herring embryos also highly susceptible to toxicity chemicals leaching stranded intertidal and subtidal zones. The water-soluble components crude trigger an adverse outcome pathway involves disruption the physiological functions cardiomyocytes embryonic heart. In previous studies, impaired ionoregulation (calcium potassium cycling) response specific polycyclic aromatic hydrocarbons (PAHs) corresponds lethal embryolarval heart failure or subtle chamber malformations high low ends PAH exposure range, respectively. Sublethal cardiotoxicity, which abnormal outgrowth (ballooning) cardiac ventricular soon after hatching, subsequently compromises juvenile structure function, leading pathological hypertrophy ventricle reduced individual fitness, measured as cardiorespiratory performance. Previous studies have not established threshold for these sublethal delayed-in-time effects, even with total (∑)PAH exposures 29 ng/g wet weight (tissue dose). Here, we extend earlier findings showing (1) cyp1a gene expression provides metric is more sensitive than typical quantitation PAHs via GC-MS (2) morphometrics provide similarly measure toxic response. Early life stage injury (impaired development) thus occurs below limits both water tissues conventional basis assessing oil-induced losses coastal ecosystems.

Language: Английский

Strategy, Design, and Fabrication of Electrochemical Biosensors: A Tutorial DOI
Karthika Sankar,

Uroš Kuzmanović,

Scott E. Schaus

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(5), P. 2254 - 2274

Published: April 18, 2024

Advanced healthcare requires novel technologies capable of real-time sensing to monitor acute and long-term health. The challenge relies on converting a quantitative biological chemical signal into desired measurable output. Given the success in detecting glucose commercialization glucometers, electrochemical biosensors continue be mainstay academic industrial research activities. Despite wealth literature biosensors, reports are often specific particular application (e.g., pathogens, cancer markers, glucose, etc.), most fail convey underlying strategy design, if it is transferable detection different analyte. Here we present tutorial review for those entering this area that summarizes basic techniques utilized as well discusses designs optimization strategies employed improve sensitivity maximize

Language: Английский

Citations

27
The Potential Harmful Effects of Genetically Engineered Microorganisms (GEMs) on the Intestinal Microbiome and Public Health DOI Creative Commons
Aaron Lerner, Carina Benzvi, Aristo Vojdani

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(2), P. 238 - 238

Published: Jan. 23, 2024

Gut luminal dysbiosis and pathobiosis result in compositional biodiversified alterations the microbial host co-metabolites. The primary mechanism of bacterial evolution is horizontal gene transfer (HGT), acquisition new traits can be achieved through exchange mobile genetic elements (MGEs). Introducing genetically engineered microbes (GEMs) might break harmonized balance intestinal compartment. present objectives are: 1. To reveal role played by GEMs’ transfers changing landscape enteric microbiome eubiosis 2. expand on potential detrimental effects those changes human genome health. A search articles published PubMed/MEDLINE, EMBASE, Scielo from 2000 to August 2023 using appropriate MeSH entry terms was performed. exchanges induce multiple diseases. GEMs change long-term natural pro- or eukaryotic cell inhabitants. worldwide regulatory authority’s safety control not enough protect public Viability, biocontainment, many other aspects are only partially controlled harmful consequences for health should avoided. It important remember that prevention most cost-effective strategy primum non nocere focus.

Language: Английский

Citations

9
Genetic circuits for metabolic flux optimization DOI
Xianhao Xu, Xueqin Lv,

Xinyu Bi

et al.

Trends in Microbiology, Journal Year: 2024, Volume and Issue: 32(8), P. 791 - 806

Published: March 12, 2024

Language: Английский

Citations

8
Applications and Tuning Strategies for Transcription Factor-Based Metabolite Biosensors DOI Creative Commons
Gloria J. Zhou, Fuzhong Zhang

Biosensors, Journal Year: 2023, Volume and Issue: 13(4), P. 428 - 428

Published: March 28, 2023

Transcription factor (TF)-based biosensors are widely used for the detection of metabolites and regulation cellular pathways in response to metabolites. Several challenges hinder direct application TF-based sensors new hosts or metabolic pathways, which often requires extensive tuning achieve optimal performance. These strategies can involve transcriptional translational control depending on parameter interest. In this review, we highlight recent engineering obtain desired performance discuss additional design considerations that may influence a biosensor's We also examine applications these suggest important areas further work continue advancement small-molecule biosensors.

Language: Английский

Citations

16
Advances in the dynamic control of metabolic pathways in Saccharomyces cerevisiae DOI Creative Commons

Chufan Xiao,

Yuyang Pan,

Mingtao Huang

et al.

Engineering Microbiology, Journal Year: 2023, Volume and Issue: 3(4), P. 100103 - 100103

Published: June 21, 2023

The metabolic engineering of

Language: Английский

Citations

15
The Potential Harmful Effects of Genetically Engineered Microorganisms (GEMs) on the Intestinal Microbiome and Public Health DOI Open Access
Aaron Lerner, Carina Benzvi, Aristo Vojdani

et al.

Published: Jan. 3, 2024

Gut luminal dysbiosis and pathobiosis result in compositional biodiversify alterations the microbial host co-metabolites. The main mechanism of bacterial evolution is horizontal gene transfer (HGT) acquisition new traits can be achieved through mobile genetic element (MGEs) exchange. Introducing genetically engineered microbes (GEMs) might break harmonized balance intestinal compartment. present objectives are: 1. To reveal role played by GEMs' transfers changing landscape enteric microbiome eubiosis 2. expand on potential detrimental effects those changes human genome health. A search articles published PubMed/MEDLINE, EMBASE, Scielo from 2000 to august 2023, using appropriate MeSH entry terms were performed. exchanges induce multiple diseases. GEMs change long-term natural pro- or eukaryotic cells inhabitants. worldwide regulatory authority's safety control not enough protect public Viability biocontainment many other aspects are only partially controlled harmful consequences for health should avoided. Always remember that prevention most cost- effective strategy "primum non nocere" name game.

Language: Английский

Citations

5
Tuning the performance of a TphR-based terephthalate biosensor with a design of experiments approach DOI Creative Commons
Guadalupe Alvarez Gonzalez, Micaela Chacón,

Thomas Butterfield

et al.

Metabolic Engineering Communications, Journal Year: 2024, Volume and Issue: 19, P. e00250 - e00250

Published: Nov. 6, 2024

Transcription factor-based biosensors are genetic tools that aim to predictability link the presence of a specific input stimuli tailored gene expression output. The performance characteristics biosensor fundamentally determines its potential applications. However, current methods engineer and optimise responses highly nonintuitive, struggle investigate multidimensional sequence/design space efficiently. In this study we employ design experiments (DoE) approach build framework for efficiently engineering activator-based with performances, apply development polyethylene terephthalate (PET) plastic degradation monomer (TPA). We simultaneously core promoter operator regions responsive promoter, by employing dual refactoring approach, were able explore an enhanced assign their causative effects. employed here serves as foundational transcriptional enabled dynamic range diverse signal output, sensitivity, steepness. further demonstrate applicability on primary screening PET hydrolases enzyme condition screening, demonstrating statistical modelling in optimising industrial environmental

Language: Английский

Citations

5
Fundamentals of Biosensors DOI

Fatma M. Shahat,

Donia G. Youssef,

Fatma E. Sayed

et al.

Published: Jan. 1, 2024

Citations

4
AI-Assisted Rational Design and Activity Prediction of Biological Elements for Optimizing Transcription-Factor-Based Biosensors DOI Creative Commons

Nana Ding,

Zenan Yuan,

Zheng Ma

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(15), P. 3512 - 3512

Published: July 26, 2024

The rational design, activity prediction, and adaptive application of biological elements (bio-elements) are crucial research fields in synthetic biology. Currently, a major challenge the field is efficiently designing desired bio-elements accurately predicting their using vast datasets. advancement artificial intelligence (AI) technology has enabled machine learning deep algorithms to excel uncovering patterns bio-element data performance. This review explores AI design bio-elements, regulation transcription-factor-based biosensor response performance AI-designed elements. We discuss advantages, adaptability, challenges addressed by various applications, highlighting powerful potential analyzing data. Furthermore, we propose innovative solutions faced suggest future directions. By consolidating current demonstrating practical applications biology, this provides valuable insights for advancing both academic biotechnology.

Language: Английский

Citations

4
Fundamentals and Exceptions of the LysR-type Transcriptional Regulators DOI
Wouter Demeester,

Brecht De Paepe,

Marjan De Mey

et al.

ACS Synthetic Biology, Journal Year: 2024, Volume and Issue: 13(10), P. 3069 - 3092

Published: Sept. 22, 2024

LysR-type transcriptional regulators (LTTRs) are emerging as a promising group of macromolecules for the field biosensors. As largest family bacterial transcription factors, LTTRs represent vast and mostly untapped repertoire sensor proteins. To fully harness these factor-based biosensor development, it is crucial to understand their underlying mechanisms functionalities. In first part, this Review discusses established model features LTTRs. dual-function regulators, inducible factors exude precise control over regulatory targets. second part Review, an overview given exceptions "classic" LTTR model. While general mechanism has helped elucidate intricate regulation performed by LTTRs, essential recognize variations within family. By combining knowledge, characterization new can be done more efficiently accurately, accelerating expansion sensors development. Unlocking pool would significantly expand currently limited range detectable molecules functions available implementation novel synthetic genetic circuitry.

Language: Английский

Citations

4