Trends in biotechnology: <em>Vibrio natriegens</em> as potential micro-factory for valorization of crustacean waste DOI Open Access
Thanh Hung Nguyen, Trần Thị Vân Anh,

Thuy-Hang Dam

et al.

ACADEMIA JOURNAL OF BIOLOGY, Journal Year: 2024, Volume and Issue: 46(4), P. 71 - 89

Published: Dec. 27, 2024

Vibrio natriegens has recently been identified as a promising host for the biotechnology industry thanks to its inherent qualities, which include fast growth rate, non-pathogenicity humans, and versatility in using substrates. These advantages have led potential use of V. biosynthesis several products. Basically, industrial scale requires fermentation or cultivation processes be conducted at high substrate biomass concentrations maximize final retrieved product. However, studies on cell density are limited. Besides, convert recalcitrant substrates such chitin derivatives into biological products not yet understood. This review summarizes up-to-date information physiological characteristics, metabolism, genome, genetic modification tools natriegens. Subsequentially, statistics analysis research trends related was presented. Finally, discussion role converting waste from seafood processing culturing feedstock achieve circular economy net zero emissions provided.

Language: Английский

De novo production of eumelanin in a fermentative process with Corynebacterium glutamicum DOI
Eldin Kurpejović, Ceren Karcioglu, Emre Abdullahoglu

et al.

Biocatalysis and Biotransformation, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 14

Published: April 7, 2025

Language: Английский

Citations

0
Metabolic Engineering for the Biosynthesis of Pentalenene in the Rapidly Growing Bacterium Vibrio natriegens DOI Creative Commons
Lujun Hu, Rui Lin, Hui Jiang

et al.

Fermentation, Journal Year: 2025, Volume and Issue: 11(5), P. 249 - 249

Published: May 1, 2025

Vibrio natriegens (V. natriegens) is an emerging synthetic biology chassis characterized by rapid growth, and its potential for the synthesis of sesquiterpenes (such as pentalenene) has not been developed. In this study, heterologous pentalenene biosynthesis was successfully established in V. via metabolic engineering. The optimization gene dosage culture conditions led to increase yield from 0.75 mg/L 39.4 mg/L, representing highest titer reported date, though still markedly lower than yields achieved conventional microbial hosts. Transcriptome analysis demonstrated that exogenous mevalonate (MVA) pathway effectively activated terpenoid precursor synthesis, evidenced up-regulation key genes. However, endogenous methylerythritol 4-phosphate (MEP) remained inactive, genes involved oxidative phosphorylation, pentose phosphate pathway, thiamine were down-regulated, leading limited availability ATP, NADPH, acetyl-CoA. Competition cofactors, particularly further constrained supply efficiency. This study confirmed a production platform revealed bottleneck, providing theoretical basis subsequent engineering optimization.

Language: Английский

Citations

0
Purity of Biosynthesized Eumelanin via Solid-State Nuclear Magnetic Resonance DOI Creative Commons

Nishani K. Jayakody,

Christopher A. Klug,

Mark O. Bovee

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: May 6, 2025

Language: Английский

Citations

0
The new chassis in the flask: Advances in Vibrio natriegens biotechnology research DOI Creative Commons

Matthew Lima,

Charandatta Muddana,

Zhengyang Xiao

et al.

Biotechnology Advances, Journal Year: 2024, Volume and Issue: 77, P. 108464 - 108464

Published: Oct. 9, 2024

Biotechnology has been built on the foundation of a small handful well characterized and well-engineered organisms. Recent years have seen breakout performer gain attention as new entrant into bioengineering toolbox: Vibrio natriegens. This review covers recent research efforts making V. natriegens biotechnology platform, using large language model (LLM) knowledge graph to expedite literature survey process. Scientists made advancements in pertaining fundamental metabolic characteristics natriegens, development characterization synthetic biology tools, systems analysis modeling, bioproduction engineering, microbial ecology. Each these subcategories relevance future for applications. In this review, we cover offer context impact they may field, highlighting benefits drawbacks organism. From examining research, it appears that is precipice becoming platform bacterium biotechnology.

Language: Английский

Citations

3
Engineering xylose induction in Vibrio natriegens for biomanufacturing applications DOI Creative Commons
Eric VanArsdale, Erin E. Kelly, Cameron V. Sayer

et al.

Biotechnology and Bioengineering, Journal Year: 2024, Volume and Issue: 121(11), P. 3572 - 3581

Published: July 19, 2024

Xylose is an abundant, inexpensive and readily available carbohydrate common in minimally processed feedstocks such as seaweed algae. While a wide variety of marine microbes have evolved to utilize algae, only few currently the requisite characteristics genetic engineering tools necessary entertain use these underutilized feedstocks. The rapidly growing Gram-negative halophilic bacterium Vibrio natriegens one chassis. In this study, we engineered tested xylose induction V. tool for scalable bioproduction applications. First, created sensing construct based on operon from Escherichia coli MG1665 measured its activity using fluorescent reporter identified that cellular import plays key role strength expression required XylR transcription factor. Next, select deletions promoter region enhance gene expression, limiting effect repression when used inducer presence industrially relevant carbon sources. Lastly, optimized constructs produce biopolymer melanin seawater mimetic media. One formulations utilized nori-based extract demonstrated yields comparable previously methods more traditional costly inducer. Together, results demonstrate can provide effective lower cost option timed biosynthesis biomanufacturing applications renewable

Language: Английский

Citations

2
Vibrio natriegens: Application of a Fast-Growing Halophilic Bacterium DOI
Maurice Hädrich, Clarissa Schulze, Josef Hoff

et al.

Advances in biochemical engineering, biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

Citations

1
Natural transformation of Vibrio natriegens with large genetic cluster enables alginate assimilation for isopentenol production DOI

Yungyu Lee,

Keonwoo Kim, Mincheol Choi

et al.

Bioresource Technology, Journal Year: 2024, Volume and Issue: 406, P. 130988 - 130988

Published: June 15, 2024

Language: Английский

Citations

0
Trends in biotechnology: <em>Vibrio natriegens</em> as potential micro-factory for valorization of crustacean waste DOI Open Access
Thanh Hung Nguyen, Trần Thị Vân Anh,

Thuy-Hang Dam

et al.

ACADEMIA JOURNAL OF BIOLOGY, Journal Year: 2024, Volume and Issue: 46(4), P. 71 - 89

Published: Dec. 27, 2024

Vibrio natriegens has recently been identified as a promising host for the biotechnology industry thanks to its inherent qualities, which include fast growth rate, non-pathogenicity humans, and versatility in using substrates. These advantages have led potential use of V. biosynthesis several products. Basically, industrial scale requires fermentation or cultivation processes be conducted at high substrate biomass concentrations maximize final retrieved product. However, studies on cell density are limited. Besides, convert recalcitrant substrates such chitin derivatives into biological products not yet understood. This review summarizes up-to-date information physiological characteristics, metabolism, genome, genetic modification tools natriegens. Subsequentially, statistics analysis research trends related was presented. Finally, discussion role converting waste from seafood processing culturing feedstock achieve circular economy net zero emissions provided.

Language: Английский

Citations

0