Direct Scaffold-Coupled Electrical Stimulation of Chondrogenic Progenitor Cells through Graphene Foam Bioscaffolds to Control Mechanical Properties of Graphene Foam – Cell Composites DOI Creative Commons

Mone’t Sawyer,

Amevi Semodji,

Olivia Nielson

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 24, 2024

Abstract Osteoarthritis, a major global cause of pain and disability, is driven by the irreversible degradation hyaline cartilage in joints. Cartilage tissue engineering presents promising therapeutic avenue, but success hinges on replicating native physiological environment to guide cellular behavior generate constructs that mimic natural cartilage. Although electrical stimulation has been shown enhance chondrogenesis extracellular matrix production 2D cultures, mechanisms underlying these effects remain poorly understood, particularly 3D models. Here, we report direct scaffold-coupled applied graphene foam bioscaffolds significantly enhances mechanical properties resulting – cell constructs. Using custom 3D-printed stimulus chambers, biphasic square impulses (20, 40, 60 mVpp at 1 kHz) for 5 minutes daily over 7 days. Stimulation increased steady-state energy dissipation equilibrium modulus approximately 65% 25%, respectively, compared unstimulated controls, while also yielding highest density among stimulated samples. In addition, our chambers facilitated full submersion hydrophobic media, leading enhanced attachment integration across scaffold surface within its hollow branches. To assess this integration, employed co-localized confocal fluorescence microscopy X-ray microCT imaging enabled colloidal gold nanoparticle fluorophore staining, which allowed visualization distribution opaque scaffold’s internal structure. These findings highlight potential modulate engineered tissues offer new insights into emergent cells conductive bioscaffolds.

Language: Английский

The Role of Polysaccharide-based Biodegradable Soft Polymers in the Healthcare Sector DOI Creative Commons
Zia Ullah Arif

Advanced Industrial and Engineering Polymer Research, Journal Year: 2024, Volume and Issue: unknown

Published: May 1, 2024

Bio-based polymers have garnered significant interest across the manufacturing industry, global economy, and various engineering disciplines such as packaging, tissue engineering, controlled drug delivery, wound dressing, textiles. In current era, bio-based polymers, notably polysaccharides, offer a promising platform for constructing intricate versatile structures in biomedical sector. These encompass applications regenerative medicine (TERM), delivery devices, coatings wearable sensors, thanks to their distinctive features inherent biocompatibility, flexibility, stretchability, mechanical strength, renewability, physiological activity, favorable biological environment. this review, we concise overview of diverse types polysaccharide-based composites, properties, interactions with specific cells tissues. The review encompasses recent progress scaffolds designed cartilage, skin, neural, vascular, cardiac, bone regeneration, employing both conventional modern techniques. Additionally, it delves into development other biodegradable including systems (DDSs), antibacterial on medical electronic devices healthcare Furthermore, also elucidates research directions future perspectives while emphasizing importance regulatory approvals commitment environmental sustainability. This well-organized critical is expected assist practitioners researchers gaining deeper understanding trends, challenges, potential solutions, thereby harnessing immense biomaterials system. utilization polysaccharides sector aligns principles nature, contributing reduction carbon dioxide emissions supporting Sustainable Development Goals United Nations.

Language: Английский

Citations

17
Therapeutic applications of biological macromolecules and scaffolds for skeletal muscle regeneration: A review DOI
Syed Sayeed Ahmad, Khurshid Ahmad, Jeong Ho Lim

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 267, P. 131411 - 131411

Published: April 6, 2024

Language: Английский

Citations

5
PoreVision: A Program for Enhancing Efficiency and Accuracy in SEM Pore Analyses of Gels and Other Porous Materials DOI Creative Commons
Levi M. Olevsky, M Jacques, Katherine R. Hixon

et al.

Gels, Journal Year: 2025, Volume and Issue: 11(2), P. 132 - 132

Published: Feb. 13, 2025

Porous gels are frequently utilized as cell scaffolds in tissue engineering. Previous studies have highlighted the significance of scaffold pore size and orientation influencing migration differentiation. Moreover, there exists a considerable body research focused on optimizing characteristics to enhance performance. However, current methods for numerical characterization typically involve expensive machines or manual measurements using image manipulation software. In this project, our objective is develop user-friendly, versatile, freely accessible software tool Python scripting. This aims streamline objectify characterization, thereby accelerating efforts providing standardized framework researchers working with porous gels. Our group found that first-time users PoreVision ImageJ take similar amounts time use both programs; however, capable handling larger datasets reduced variability. Further, exhibited lower variability area compared ImageJ, while perimeter was between two. showed higher average measurements, likely due its sample broader range sizes, which may be missed ImageJ's scanning approach. By facilitating quantitative analysis size, shape, orientation, will contribute more comprehensive understanding properties their impact cellular behavior. Ultimately, we aim aid field engineering user-friendly enhances reproducibility reliability analyses.

Language: Английский

Citations

0
Exploring Carbon Nanomaterials in Tissue Engineering: Biomaterials for Regeneration and Stem Cell Integration DOI

Surekha Kulwade,

Gajanan K Arbade, P.S. Patil

et al.

Polymer-Plastics Technology and Materials, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 25

Published: Feb. 23, 2025

Language: Английский

Citations

0
Prevascularized Hydrogel Enhancing Innervation and Repair of Full‐Thickness Volumetric Muscle Loss in Abdominal Wall Defects DOI Open Access

Chia‐Chang Hsieh,

Jixiang Dai, Chi‐Kung Ni

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 9, 2025

Current materials for repairing abdominal peritoneal defects face rapid degradation, infection risk, insufficient vascular ingrowth, slow muscle regeneration, and suboptimal postoperative integration, often causing fibrotic healing hindering volumetric loss (VML) repair exceeding 30%. To address these issues, photo-cross-linkable gelatin hydrogels are combined with blood vessel-forming cells to reconstruct networks, providing temporary nutrient gas channels that support cell repair. By developing a polymer-chain propagation time technique, hydrogel properties optimized, avoiding limitations of conventional light exposure. These gels guide blood-vessel formation in vitro promote robust microvessel neural development vivo. Precise control exposure times balances cross-linking fostering vessel growth host motor neuron ingrowth. In 55% VML, enable full-thickness restoring up 70% lost while mimicking healthy tissue's strength structure. Achieving higher degradation rates density 50 vessels/mm-2 is essential functional strategies effectively bridge current clinical gaps, advancing regenerative medicine. The ability fine-tune stiffness underscores hydrogels' potential as carriers, allowing the reconstruction at injury sites significantly enhancing tissue regeneration.

Language: Английский

Citations

0
Hyaluronic Acid-Coated Melt Electrowritten Scaffolds Promote Myoblast Attachment, Alignment, and Differentiation DOI Creative Commons
Alycia N. Galindo,

K. Alyssa,

Ievgenii Liashenko

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

Abstract Purpose In muscle tissues, anisotropic cell alignment is essential for optimal fiber development and function. Biomaterials tissue engineering must guide cellular while supporting proliferation myogenic differentiation. Methods Here, we describe the fabrication of a tissue-engineered construct consisting scaffold aligned poly(ε-caprolactone) (PCL) microfibers coated in dynamic covalent hydrazone crosslinked hyaluronic acid (HA) hydrogel to support myoblast attachment, alignment, Norbornene modification HA further enabled functionalization with fibronectin-derived arginine-glycine-aspartic (RGD) peptide. Scaffolds were fabricated using melt electrowriting (MEW), three-dimensional (3D)-printing technique that uses stabilization fluid columns produce precisely polymeric microfibers. We evaluated scaffolds diameters 10 µm, 20 30 µm non-coated, HA-coated, HA-RGD-coated MEW through immunocytochemistry creatine kinase activity assays. Results HA-coated showed increased attachment C2C12 mouse skeletal myoblasts on all compared non-coated scaffolds, demonstrating highest attachment. All supported along fibers. Cells differentiated myotube formation as seen by myosin heavy chain (MHC) staining. Highest (CK) day 5 signified successful differentiation cells into mature myotubes. Conclusion This unique combination tunable biophysical biochemical cues enables creation biomimetic engineered scaffold, providing platform new therapeutic approaches regeneration.

Language: Английский

Citations

0
Soft Biological Actuators for Meter-Scale Homeostatic Biohybrid Robots DOI
Ronald H. Heisser,

Maheera Bawa,

Jessica Shah

et al.

Chemical Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Skeletal muscle's elegant protein-based architecture powers motion throughout the animal kingdom, with its constituent actomyosin complexes driving intra- and extra-cellular motion. Classical motors recently developed soft actuators cannot match packing density contractility of individual muscle fibers that scale to power ants elephants alike. Accordingly, interdisciplinary fields robotics tissue engineering have combined efforts build living can a new class robots be more energy-efficient, dexterous, safe than existing motor-powered hydraulic paradigms. Doing so ethically at scale─creating meter-scale constructs from sustainable progenitor cell lines─has inspired innovations in biomaterials culture methodology. We weave discussions biology, materials chemistry, engineering, biohybrid design review state art actuator biofabrication. Looking forward, we outline vision for robotic systems tie recent progress long-term research goals.

Language: Английский

Citations

0
Advancing Prosthetic Hand Capabilities Through Biomimicry and Neural Interfaces DOI
Mohammad Haghani Dogahe, Mark A. Mahan, Miqin Zhang

et al.

Neurorehabilitation and neural repair, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Background and Objectives Prosthetic hand development is undergoing a transformative phase, blending biomimicry neural interface technologies to redefine functionality sensory feedback. This article explores the symbiotic relationship between biomimetic design principles technology (NIT) in advancing prosthetic capabilities. Methods Drawing inspiration from biological systems, researchers aim replicate intricate movements capabilities of human through innovative designs. Central this endeavor NIT, facilitating seamless communication artificial devices nervous system. Recent advances fabrication methods have propelled brain–computer interfaces, enabling precise control hands by decoding activity. Results Anatomical complexities underscore importance understanding biomechanics, neuroanatomy, mechanisms for crafting effective solutions. Furthermore, achieving goal fully functional cyborg necessitates multidisciplinary approach body’s inherent By incorporating expertise clinicians, tissue engineers, bioengineers, electronic data scientists, next generation implantable not only anatomically biomechanically accurate but also offer intuitive control, feedback, proprioception, thereby pushing boundaries current technology. Conclusion integrating machine learning algorithms, biomechatronic principles, advanced surgical techniques, can achieve real-time while restoring tactile sensation proprioception. manuscript contributes novel approaches development, with potential implications enhancing functionality, durability, safety limb.

Language: Английский

Citations

0
Simultaneous measurement of tensile and shear elasticity and anisotropy in human skeletal muscle tissue using steered ultrasound shear waves DOI Creative Commons
Ricardo J. Andrade,

Apolline Racapé,

Mar Hernández-Secorún

et al.

Acta Biomaterialia, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0
Preparation of PDA-GO/CS composite scaffold and its effects on the biological properties of human dental pulp stem cells DOI Creative Commons
Yaoyao Li, Xinhui Huang,

Weihao Fu

et al.

BMC Oral Health, Journal Year: 2024, Volume and Issue: 24(1)

Published: Jan. 31, 2024

Abstract Reduced graphene oxide (rGO) is an (GO) derivative of graphene, which has a large specific surface area and exhibited satisfactory physicochemical characteristics. In this experiment, GO was reduced by PDA to generate PDA-GO complex, then combined with Chitosan (CS) synthesize PDA-GO/CS composite scaffold. added CS improve the degradation rate CS, it hoped that scaffolds could be used in bone tissue engineering. Physicochemical antimicrobial properties different were examined find optimal mass fraction. Besides, we scaffold’s biocompatibility Phalloidin staining Live Dead fluorescent staining. Finally, applied ALP staining, RT-qPCR, Alizarin red S detect effect on osteogenic differentiation human dental pulp stem cells (hDPSCs). The results showed successfully prepared scaffold synthesized combining CS. Among them, 0.3%PDA-GO/CS improves antibacterial activity hydrophilicity while reducing rate. vitro, superior enhances early proliferation, migration hDPSCs. conclusion, new materialsuitable for cell culture promising application prospect as

Language: Английский

Citations

3