Identification of ZNF652 as a Diagnostic and Therapeutic Target in Osteoarthritis Using Machine Learning DOI Creative Commons
Yeping Chen,

Rongyuan Liang,

Xifan Zheng

et al.

Journal of Inflammation Research, Journal Year: 2024, Volume and Issue: Volume 17, P. 10141 - 10161

Published: Dec. 1, 2024

Purpose: Osteoarthritis (OA) is the most common degenerative joint disease. However, its etiology remains largely unknown. Zinc Finger Protein 652 (ZNF652) a transcription factor implicated in various biological processes. Nevertheless, role OA has not been elucidated. Methods: The search term "osteoarthritis" was utilized to procure transcriptome data relating patients and healthy people from Gene Expression Omnibus (GEO) database. Then screening process initiated identify differentially expressed genes (DEGs). DEGs were discerned using three distinct machine learning methods. accuracy of these diagnosing evaluated Receiver Operating Characteristic (ROC) Curve. A competitive endogenous RNA (ceRNA) visualization network established delve into potential regulatory targets. ZNF652 expression confirmed cartilage rats quantitative reverse polymerase chain reaction (qRT-PCR) Western blotting (WB) analyzed an independent t -test. Results: identified as DEG exhibited highest diagnostic value for according ROC analysis. GO KEGG enrichment analyses suggest that plays vital development through processes including nitric oxide anabolism, macrophage proliferation, immune response, PI3K/Akt MAPK signaling pathways. increased validated qRT-PCR (1.193 ± 0.005 vs 1.000 0.005, p < 0.001) WB (0.981 0.055 0.856 0.026, = 0.012) Conclusion: found be related pathogenesis can potentially serve therapeutic target OA. underlying mechanism pathways, cells their functions findings need clinical trials molecular requires further study. Keywords: osteoarthritis, zinc finger protein 652, algorithms, cell

Language: Английский

Research Progress in Hydrogels for Cartilage Organoids DOI

Xiaolong Li,

Shihao Sheng, Guangfeng Li

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(22)

Published: May 21, 2024

The repair and regeneration of cartilage has always been a hot topic in medical research. Cartilage organoids (CORGs) are special tissue created using engineering techniques outside the body. These engineered tissues provide models that simulate complex biological functions cartilage, opening new possibilities for regenerative medicine treatment strategies. However, it is crucial to establish suitable matrix scaffolds cultivation CORGs. In recent years, utilizing hydrogel culture stem cells induce their differentiation into chondrocytes emerged as promising method vitro construction this review, methods establishing CORGs summarized an overview advantages limitations matrigel such provided. Furthermore, importance ECM alternative substitutes Matrigel, alginate, peptides, silk fibroin, DNA derivatives discussed, pros cons these hydrogels outlined. Finally, challenges future directions research discussed. It hoped article provides valuable references design development

Language: Английский

Citations

23
Pyroptosis in Osteoarthritis: Molecular Mechanisms and Therapeutic Implications DOI Creative Commons
Yeping Chen,

Daofu Zeng,

Guizheng Wei

et al.

Journal of Inflammation Research, Journal Year: 2024, Volume and Issue: Volume 17, P. 791 - 803

Published: Feb. 1, 2024

Osteoarthritis (OA) is a chronic disease that causes pain and functional impairment by affecting joint tissue. Its global impact noteworthy, causing significant economic losses property damage. Despite extensive research, the underlying pathogenesis of OA remain an area ongoing investigation. It has recently been discovered progression significantly influenced pyroptosis. Pyroptosis complex process involves three pathways culminating in assembly Gasdermin-D (GSDMD)-N-terminal (GSDMD-NT) into pores through aggregation on plasma membrane. The GSDMD-NT proteins stimulates release inflammatory mediators, such as Interleukin-1β (IL-1β), Interleukin-18 (IL-18), Matrix Metallopeptidase 13 (MMP13), ultimately leading to cellular lysis. pyroptosis specific cells, including synovial macrophages, fibroblast-like synoviocytes (FLS), chondrocytes, subchondral osteoblasts, contributs factor development OA. Currently, cells undergo first are not yet fully understood, it remains unknown whether one cell can trigger same other cells. Therefore, targeting could potentially offer novel treatment approach for patients. We present comprehensive analysis molecular mechanisms key features also outline current research progress various aspects, tissue, articular cartilage, extracellular matrix (ECM), bone, with focus aim provide theoretical references effective management

Language: Английский

Citations

11
Identification of a novel disulfidptosis-related gene signature in osteoarthritis using bioinformatics analysis and experimental validation DOI Creative Commons

Mingjie Wei,

Xiangwen Shi,

Wenbao Tang

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 8, 2025

Osteoarthritis (OA) is a degenerative bone disease characterized by the destruction of joint cartilage and synovial inflammation, involving intricate immune regulation processes. Disulfidptosis, novel form programmed cell death, has recently been identified; however, effects roles disulfidptosis-related genes (DR-DEGs) in OA remain unclear. We obtained six datasets from GEO database, using four as training sets two validation sets. Differential expression analysis was employed to identify DR-DEGs, unique molecular subtypes were constructed based on these DR-DEGs. Subsequently, microenvironment patients comprehensively analyzed "CIBERSORT" algorithm for infiltration. Various machine learning algorithms utilized screen characteristic nomogram models ROC curves built genes. The scRNA dataset (GSE169454) used classify chondrocytes samples into distinct types, further exploring gene distribution correlation DR-DEGs with specific subpopulations. Moreover, levels validated through rat models. In our study, we identified 10 significant differences within samples. Based recognized (cluster 1 2). ZNF484 NDUFS1 found be significantly overexpressed subtype 1, while infiltration abundance activated mast cells markedly elevated 2. observed proportions 11 types between control samples, 9 demonstrating substantial correlations levels. Further revealed that SLC3A2 NDUFC1 predominantly expressed preHTC subpopulation. All exhibited notably higher EC subpopulation across various types. proportion subgroups high increased, mainly enriching pathways related such IL-17 signaling pathway TGF-beta pathway. Using learning, which, combination models, demonstrated promising performance diagnosis OA. Additionally, vivo confirmed elevation PPM1F This study potential biomarkers classification provided preliminary understanding their role microenvironment. However, experimental clinical studies are required validate diagnostic value therapeutic potential.

Language: Английский

Citations

1
Effectiveness of phytoconstituents and potential of phyto-nanomedicines combination to treat osteoarthritis DOI Creative Commons
Laxmi Akhileshwar Jha, Mohammad Imran, Jesus Shrestha

et al.

European Polymer Journal, Journal Year: 2024, Volume and Issue: 215, P. 113243 - 113243

Published: July 1, 2024

Osteoarthritis (OA) is a well-known degenerative joint disease recognized by the deterioration of cartilage in joints, leading to pain and reduced mobility. Traditional treatments for OA include management, physical therapy, severe cases, replacement surgery. In recent years, there has been growing interest exploring potential phytoconstituents nanomedicines combined treating OA. Furthermore, with increasing amount study this field, now opportune time widespread use plant-derived medications as complementary alternative medical therapies be acknowledged used more efficient treatment human ailments like Combining nanomedicine technology (phyto-nanomedicine) can potentially enhance their effectiveness The phyto-nanomedicines many advantages, including enhanced permeability, increased bioavailability, sustained/controlled drug release at site, decreased adverse effects, possible combination treatment. It's important note that while promising preclinical some clinical evidence regarding phyto-nanomedicine treatment, further research needed establish safety efficacy conclusively. review, treat combining phytomedicines nanoparticulate delivery former therapeutic discussed detail. we have also described briefly on application organ-on-chip and/or on-chip models accelerate identification novel evaluate potency

Language: Английский

Citations

7
Bone and Joint‐on‐Chip Platforms: Construction Strategies and Applications DOI
Chengcheng Du, Jiacheng Liu, Senrui Liu

et al.

Small Methods, Journal Year: 2024, Volume and Issue: unknown

Published: May 19, 2024

Organ-on-a-chip, also known as "tissue chip," is an advanced platform based on microfluidic systems for constructing miniature organ models in vitro. They can replicate the complex physiological and pathological responses of human organs. In recent years, development bone joint-on-chip platforms aims to simulate processes occurring bones joints, including cell-cell interactions, interplay various biochemical factors, effects mechanical stimuli, intricate connections between multiple future, will integrate advantages disciplines, bringing more possibilities exploring disease mechanisms, drug screening, personalized medicine. This review explores construction application Organ-on-a-chip technology joint research, proposes a modular concept, discusses new opportunities future challenges platforms.

Language: Английский

Citations

6
ABCA6 Regulates Chondrogenesis and Inhibits Joint Degeneration via Orchestrated Cholesterol Efflux and Cellular Senescence DOI Creative Commons
Yi Wang, Qiang Wu, Yongqing You

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 17, 2025

Patellar dysplasia (PD) can cause patellar dislocation and subsequent osteoarthritis (OA) development. Herein, a novel ABCA6 mutation contributing to four-generation family with familiar (FPD) is identified. In this study, whole exome sequencing (WES) genetic linkage analysis across lineage presenting six cases of FPD are conducted. A disease-causing in identified for FPD. Further analyses reveal consistent correlation between expression downregulation PD occurrence, chondrocyte degeneration, OA onset. Moreover, ABCA6-KO mice demonstrate severe knee joint degeneration accelerated progression. Besides, synovial mesenchymal stem cells (SMSCs) extracted from WT, ABCA6-/+, ABCA6-/- create chondrogenic organoids vitro, confirming deficiency lead via modulating cell cycle activating cellular senescence. transcriptome metabolomic on chondrocytes unveils that the inhibits cholesterol efflux, leading intracellular accumulation senescence impaired chondrogenesis.A correlated occurrence serve as potential target chondrogenesis treatment by orchestrated efflux delayed

Language: Английский

Citations

0
Cartilage repair: unleashing PRP’s potential in organoid models DOI

Mehdi Golshan,

Hengameh Dortaj, Zahra Omidi

et al.

Cytotechnology, Journal Year: 2025, Volume and Issue: 77(3)

Published: April 3, 2025

Language: Английский

Citations

0
Biomimetic-smart materials for osteochondral regeneration and repair DOI Open Access

Qi-Peng Jia,

Qunfeng Li,

Hamza Boucetta

et al.

Microstructures, Journal Year: 2024, Volume and Issue: 4(3)

Published: May 14, 2024

Osteochondral injuries represent prevalent clinical conditions with profound implications for functional impairment and diminished quality of life. Despite the considerable potential tissue engineering in osteochondral repair, substantial strides implementation remain elusive. Biomimetic materials, designed to emulate natural cartilage, offer a stabilized structure microenvironment capable accommodating diverse properties inherent different cartilage regions. Smart endowed ability deliver drugs, metal ions, growth factors contingent on disease progression, exert precise control over cellular epigenetic behaviors. This review scrutinizes nuanced characteristics both physiological pathological states. Subsequently, succinct overview recent applications biomaterials bionic smart attributes regeneration repair is provided. Finally, we propose our perspectives application biomimetic-smart materials emphasizing their translation.

Language: Английский

Citations

1
CircMYO1C silencing alleviates chondrocytes inflammation and apoptosis through m6A/HMGB1 axis in osteoarthritis DOI
Haitao Sun,

Xudong Chu,

Weiqing Qian

et al.

Biotechnology and Applied Biochemistry, Journal Year: 2024, Volume and Issue: unknown

Published: July 9, 2024

Circular RNAs (circRNAs) are involved in osteoarthritis (OA) progression. This study aimed to investigate the role and molecular mechanisms of circMYO1C OA. CircMYO1C was upregulated OA- interleukin-1β (IL-1β)-exposed chondrocytes. The results indicated that knockdown repressed inflammatory factors (tumor necrosis factor alpha [TNF-α], interleukin-6 [IL-6], interleukin-8 [IL-8], etc.) apoptosis chondrocytes following IL-1β exposure. an N

Language: Английский

Citations

0
Modeling organoids in the context of rheumatoid arthritis DOI
Haiyang Wang, Shiyu Du, Xiaohu Tang

et al.

International Journal of Rheumatic Diseases, Journal Year: 2024, Volume and Issue: 27(9)

Published: Sept. 1, 2024

Language: Английский

Citations

0