The FASEB Journal,
Journal Year:
2024,
Volume and Issue:
38(24)
Published: Dec. 13, 2024
Abstract
Infectious
diseases
are
a
serious
threat
to
the
existence
of
animals
and
humans'
life.
In
21st
century,
emergence
re‐emergence
several
zoonotic
non‐zoonotic
global
pandemic
socio‐economic
importance
has
affected
billions
humans
animals.
The
need
for
expensive
equipment
laboratories,
non‐availability
on‐site
testing
abilities,
with
time‐consuming
low
sensitivity
specificity
issues
currently
available
diagnostic
techniques
identify
these
pathogenic
micro‐organisms
on
large
scale
highlighted
developing
cheap,
portable
environment
friendly
methods.
recent
years,
have
been
addressed
by
clustered
regularly
interspaced
palindromic
repeats
(CRISPR)‐based
platforms
that
transformed
molecular
field
due
their
outstanding
ultra‐sensitive
nucleic
acid
detecting
capabilities.
this
study,
we
highlight
types,
potential
different
Cas
proteins,
amplification
systems.
We
also
illuminate
application
CRISPR
integrated
setups
diagnosis
infectious
diseases,
majorly
in
food‐producing
(pigs,
ruminants,
poultry,
aquaculture),
domestic
pets
(dogs
cats),
importance.
conclude
challenges
future
perspectives
using
systems
rapidly
diagnose
treat
other
develop
control
strategies
prevent
spread
organisms.
MicrobiologyOpen,
Journal Year:
2024,
Volume and Issue:
13(2)
Published: March 31, 2024
Abstract
Microbial
products
are
essential
for
developing
various
therapeutic
agents,
including
antibiotics,
anticancer
drugs,
vaccines,
and
enzymes.
Genetic
engineering
techniques,
functional
genomics,
synthetic
biology
unlock
previously
uncharacterized
natural
products.
This
review
highlights
major
advances
in
microbial
biotechnology,
focusing
on
gene‐based
technologies
medical
applications.
Current Issues in Molecular Biology,
Journal Year:
2024,
Volume and Issue:
46(5), P. 4147 - 4185
Published: April 30, 2024
Recent
years
have
witnessed
unprecedented
progress
in
therapeutic
gene
editing,
revolutionizing
the
approach
to
treating
genetic
disorders.
In
this
comprehensive
review,
we
discuss
progression
of
milestones
leading
emergence
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)-based
technology
as
a
powerful
tool
for
precise
and
targeted
modifications
human
genome.
CRISPR-Cas9
nuclease,
base
prime
editing
taken
center
stage,
demonstrating
remarkable
precision
efficacy
ex
vivo
genomic
modifications.
Enhanced
delivery
systems,
including
viral
vectors
nanoparticles,
further
improved
efficiency
safety
advancing
their
clinical
translatability.
The
exploration
CRISPR-Cas
systems
beyond
commonly
used
Cas9,
such
development
Cas12
Cas13
variants,
has
expanded
repertoire
tools,
enabling
more
intricate
interventions.
Outstandingly,
represents
significant
leap
forward,
given
its
unparalleled
versatility
minimization
off-target
effects.
These
innovations
paved
way
multitude
previously
incurable
disorders,
ranging
from
monogenic
diseases
complex
polygenic
conditions.
This
review
highlights
latest
innovative
studies
field,
emphasizing
breakthrough
technologies
preclinical
trials,
applications
realm
medicine.
However,
challenges
effects
ethical
considerations
remain,
necessitating
continued
research
refine
profiles
frameworks.
Clinical and Translational Medicine,
Journal Year:
2025,
Volume and Issue:
15(2)
Published: Feb. 1, 2025
Abstract
Background
Myotonic
dystrophy
type
1
(DM1)
is
a
genetic
multisystemic
disease,
characterised
by
pleiotropic
symptoms
that
exhibit
notable
variability
in
severity,
nature
and
age
of
onset.
The
cause
DM1
the
expansion
unstable
CTG‐repeats
3′
untranslated
region
(UTR)
DMPK
gene,
resulting
accumulation
toxic
CUG‐transcripts
sequester
RNA‐binding
proteins
form
nuclear
foci
affected
tissues
and,
consequently,
alter
various
cellular
processes.
Therapeutic
gene
editing
for
treatment
monogenic
diseases
powerful
technology
could
principle
remove
definitively
disease‐causing
defect.
precision
efficiency
molecular
mechanisms
are
still
under
investigation
view
possible
use
clinical
practice.
Methods
Here,
we
describe
application
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)/CRISPR‐associated
protein
9
(Cas9)
strategy
to
CTG‐expansion
mouse
model
carrying
human
transgene
from
patient.
To
optimise
vivo,
identified
new
tools
allowed
improve
expression
levels
activity
CRISPR/Cas9
machinery.
Newly
designed
guide
RNA
pairs
were
tested
DM1‐patient
derived
cells
before
vivo
application.
Edited
analysed
assess
occurrence
off‐target
accuracy
on‐target
genomic
events.
Gene
editing‐dependent
‐independent
leading
decreased
mutated
transcripts
also
evaluated.
Results
Conclusion
Systemic
delivery
components
mice,
through
myotropic
adeno‐associated
viral
vectors,
led
significant
improvement
alterations
heart
skeletal
muscle.
Importantly,
persistent
increase
body
weight,
muscle
strength
composition
parameters
observed
treated
animals.
Accurate
evaluation
CRISPR/Cas9‐mediated‐phenotypic
recovery
crucial
preclinical
step
development
therapy
patients.
Key
points
In
therapeutic
permanent
deletion
pathogenetic
CTG‐repeat
amplification
causes
myotonic
1.
Following
treatment,
diseased
mice
show
both
phenotypic
defects.
Microorganisms,
Journal Year:
2025,
Volume and Issue:
13(3), P. 599 - 599
Published: March 5, 2025
Microbial
engineering
has
made
a
significant
breakthrough
in
pharmaceutical
biotechnology,
greatly
expanding
the
production
of
biologically
active
compounds,
therapeutic
proteins,
and
novel
drug
candidates.
Recent
advancements
genetic
engineering,
synthetic
biology,
adaptive
evolution
have
contributed
to
optimization
microbial
strains
for
applications,
playing
crucial
role
enhancing
their
productivity
stability.
The
CRISPR-Cas
system
is
widely
utilized
as
precise
genome
modification
tool,
enabling
enhancement
metabolite
biosynthesis
activation
biological
pathways.
Additionally,
biology
approaches
allow
targeted
design
microorganisms
with
improved
metabolic
efficiency
potential,
thereby
accelerating
development
new
products.
integration
artificial
intelligence
(AI)
machine
learning
(ML)
plays
vital
further
advancing
by
predicting
network
interactions,
optimizing
bioprocesses,
discovery
process.
However,
challenges
such
efficient
pathways,
ensuring
sustainable
industrial-scale
production,
meeting
international
regulatory
requirements
remain
critical
barriers
field.
Furthermore,
mitigate
potential
risks,
it
essential
develop
stringent
biocontainment
strategies
implement
appropriate
oversight.
This
review
comprehensively
examines
recent
innovations
analyzing
key
technological
advancements,
challenges,
future
perspectives.
Frontiers in Genome Editing,
Journal Year:
2025,
Volume and Issue:
7
Published: March 10, 2025
Climate
change
threatens
global
crop
yield
and
food
security
due
to
rising
temperatures,
erratic
rainfall,
increased
abiotic
stresses
like
drought,
heat,
salinity.
Gene
editing
technologies,
including
CRISPR/Cas9,
base
editors,
prime
offer
precise
tools
for
enhancing
resilience.
This
review
explores
the
mechanisms
of
these
technologies
their
applications
in
developing
climate-resilient
crops
address
future
challenges.
While
CRISPR/enables
targeted
modifications
plant
DNA,
editors
allow
direct
conversion
without
inducing
double-stranded
breaks,
enable
insertions,
deletions,
substitutions.
By
understanding
manipulating
key
regulator
genes
involved
stress
responses,
such
as
DREB,
HSP,
SOS,
ERECTA,
HsfA1,
NHX;
tolerance
can
be
enhanced
against
salt
stress.
improve
traits
related
root
development,
water
use
efficiency,
response
pathways,
heat
shock
response,
photosynthesis,
membrane
stability,
ion
homeostasis,
osmotic
adjustment,
oxidative
response.
Advancements
gene
integration
with
genomics,
phenomics,
artificial
intelligence
(AI)/machine
learning
(ML)
hold
great
promise.
However,
challenges
off-target
effects,
delivery
methods,
regulatory
barriers
must
addressed.
highlights
potential
develop
crops,
contributing
sustainable
agriculture.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(23), P. 12911 - 12911
Published: Nov. 30, 2024
Cartilage
degeneration
is
a
key
feature
of
aging
and
osteoarthritis,
characterized
by
the
progressive
deterioration
joint
function,
pain,
limited
mobility.
Current
treatments
focus
on
symptom
relief,
not
cartilage
regeneration.
Mesenchymal
stromal
cells
(MSCs)
offer
promising
therapeutic
option
due
to
their
capability
differentiate
into
chondrocytes,
modulate
inflammation,
promote
tissue
This
review
explores
potential
MSCs
for
regeneration,
examining
biological
properties,
action
mechanisms,
applications
in
preclinical
clinical
settings.
derived
from
bone
marrow,
adipose
tissue,
other
sources
can
self-renew
multiple
cell
types.
In
cartilage,
they
aid
regeneration
secreting
growth
factors
cytokines
that
enhance
repair
immune
responses.
Recent
studies
show
restore
integrity,
reduce
improve
although
translation
remains
challenging
limitations
such
as
viability,
scalability,
regulatory
concerns.
Advancements
MSC
delivery,
including
scaffold-based
approaches
engineered
exosomes,
may
effectiveness.
Potential
risks,
tumorigenicity
rejection,
are
also
discussed,
emphasizing
need
optimized
treatment
protocols
large-scale
trials
develop
effective,
minimally
invasive
therapies
World Journal of Gastroenterology,
Journal Year:
2024,
Volume and Issue:
30(40), P. 4339 - 4353
Published: Oct. 16, 2024
Diabetes
mellitus,
characterized
by
chronic
hyperglycemia
due
to
insulin
deficiency
or
resistance,
poses
a
significant
global
health
burden.
Central
its
pathogenesis
is
the
dysfunction
loss
of
pancreatic
beta
cells,
which
are
res-ponsible
for
production.
Recent
advances
in
beta-cell
regeneration
research
offer
promising
strategies
diabetes
treatment,
aiming
restore
endogenous
production
and
achieve
glycemic
control.
This
review
explores
physiological
basis
function,
recent
scientific
advan-cements,
challenges
translating
these
findings
into
clinical
applications.
It
highlights
key
developments
stem
cell
therapy,
gene
editing
technologies,
identification
novel
regenerative
molecules.
Despite
potential,
field
faces
hurdles
such
as
ensuring
safety
long-term
efficacy
regen-erative
therapies,
ethical
concerns
around
use,
complexity
differentiation
integration.
The
importance
interdisciplinary
collaboration,
increased
funding,
need
patient-centered
approaches
integration
new
treatments
comprehensive
care
overcome
challenges.
Through
continued
holds
potential
revolutionize
care,
turning
condition
manageable
even
curable
disease.
Microorganisms,
Journal Year:
2025,
Volume and Issue:
13(4), P. 927 - 927
Published: April 17, 2025
Metabolic
engineering
has
a
wide
range
of
applications,
spanning
key
sectors
such
as
energy,
pharmaceuticals,
agriculture,
chemicals,
and
environmental
sustainability.
Its
core
focus
is
on
precisely
modulating
metabolic
pathways
to
achieve
efficient,
sustainable,
environmentally
friendly
biomanufacturing
processes,
offering
new
possibilities
for
societal
sustainable
development.
Gene
attenuation
critical
technique
within
engineering,
pivotal
in
optimizing
fluxes
improving
target
metabolite
yields.
This
review
article
discusses
gene
mechanisms,
the
applications
across
various
biological
systems,
implementation
strategies.
Additionally,
we
address
potential
future
challenges
explore
its
drive
further
advancements
field.
