Reciprocal Effect of Environmental Stimuli to Regulate the Adipogenesis and Osteogenesis Fate Decision in Bone Marrow-Derived Mesenchymal Stem Cells (BM-MSCs)

Cells, Journal Year: 2023, Volume and Issue: 12(10), P. 1400 - 1400

Published: May 16, 2023

Mesenchymal stem cells derived from bone marrow (BM-MSCs) can differentiate into adipocytes and osteoblasts. Various external stimuli, including environmental contaminants, heavy metals, dietary, physical factors, are shown to influence the fate decision of BM-MSCs toward adipogenesis or osteogenesis. The balance osteogenesis is critical for maintenance homeostasis, interruption lineage commitment associated with human health issues, such as fracture, osteoporosis, osteopenia, osteonecrosis. This review focuses on how stimuli shift towards Future studies needed understand impact these elucidate underlying mechanisms differentiation. knowledge will inform efforts prevent bone-related diseases develop therapeutic approaches treat disorders various pathological conditions.

Language: Английский

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Bone marrow mesenchymal stem cells’ osteogenic potential: superiority or non-superiority to other sources of mesenchymal stem cells?

Cell and Tissue Banking, Journal Year: 2023, Volume and Issue: 24(3), P. 663 - 681

Published: Jan. 9, 2023

Language: Английский

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Single-Cell RNA-Seq Reveals LRRC75A-Expressing Cell Population Involved in VEGF Secretion of Multipotent Mesenchymal Stromal/Stem Cells Under Ischemia

Stem Cells Translational Medicine, Journal Year: 2023, Volume and Issue: 12(6), P. 379 - 390

Published: June 1, 2023

Abstract Human multipotent mesenchymal stromal/stem cells (MSCs) have been utilized in cell therapy for various diseases and their clinical applications are expected to increase the future. However, variation MSC-based product quality due MSC heterogeneity has resulted significant constraints utility of MSCs. Therefore, we hypothesized that it might be important identify ensure/enrich suitable subpopulations therapies using products. In this study, aimed functional predict efficacy angiogenic bone marrow-derived MSCs (BM-MSCs). To assess its potency, observed levels vascular endothelial growth factor (VEGF) secretion among 11 donor-derived BM-MSC lines under vitro ischemic culture conditions. Next, by clarifying BM-MSCs single-cell RNA-sequencing analysis, identified a subpopulation contributed overall VEGF production We also found leucine-rich repeat-containing 75A (LRRC75A) was more highly expressed than others. Importantly, knockdown LRRC75A small interfering RNA inhibition BM-MSCs, indicating regulates may useful biomarker contribute effects BM-MSCs. Our work provides evidence strategy based on transcriptome profiles is effective identifying heterogeneous

Language: Английский

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Biodegradable Tyramine Functional Gelatin/6 Arms-PLA Inks Compatible with 3D Two Photon-Polymerization Printing and Meniscus Tissue Regeneration

Biomacromolecules, Journal Year: 2024, Volume and Issue: 25(8), P. 5098 - 5109

Published: July 23, 2024

The meniscus regeneration can present major challenges such as mimicking tissue microstructuration or triggering cell regeneration. In the case of lesions that require a personalized approach, photoprinting offers possibility designing resolutive biomaterial structures. photo-cross-linkable ink composition determines process ease and final network properties. this study, we designed range hybrid inks composed gelatin(G) 6-PLA arms(P) were photo-cross-linked using tyramine groups. photo-cross-linking efficiency, mechanical properties, degradation, biological interactions with different G/P mass ratios studied. G50P50 properties suitable for regeneration, Young's modulus 6.5 MPa, degradation in 2 months, good proliferation. We then confirmed potential these to produce high-resolution microstructures by printing well-defined two-photon polymerization. These offer new perspectives biocompatible, degradable, microstructured engineering scaffold creation.

Language: Английский

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Exosomes derived from bone marrow mesenchymal stem cell preconditioned by low-intensity pulsed ultrasound stimulation promote bone–tendon interface fibrocartilage regeneration and ameliorate rotator cuff fatty infiltration

Journal of Orthopaedic Translation, Journal Year: 2024, Volume and Issue: 48, P. 89 - 106

Published: Aug. 2, 2024

Fibrovascular scar healing of bone-tendon interface (BTI) instead functional fibrocartilage regeneration is the main concern associated with unsatisfactory prognosis in rotator cuff repair. Mesenchymal stem cells (MSCs) exosomes have been reported to be a new promising cell-free approach for healing. Whereas, controversies abound whether native MSCs alone can effectively induce chondrogenesis. To explore effect derived from low-intensity pulsed ultrasound stimulation (LIPUS)-preconditioned bone marrow mesenchymal (LIPUS-BMSC-Exos) or un-preconditioned BMSCs (BMSC-Exos) on and underlying mechanism. C57BL/6 mice underwent unilateral supraspinatus tendon detachment repair were randomly assigned saline, BMSCs-Exos LIPUS-BMSC-Exos injection therapy. Histological, immunofluorescent biomechanical tests detected investigate BTI muscle fatty infiltration repaired cuff. In vitro, incubated BMSC-Exos then chondrogenic/adipogenic differentiation observed. Further, quantitative real-time polymerase chain reaction (qRT-PCR) was performed detect chondrogenesis/adipogenesis-related miRNA profiles BMSC-Exos. The potential key verified through function recover test its mimic inhibitor. results indicated that properties tendon-humeral junction significantly improved group than group. also exhibited higher histological score more newly regenerated at site postoperative 2 4 weeks less co-culture could promote chondrogenic inhibit adipogenic differentiation. Subsequently, qRT-PCR revealed enrichment miRNAs compared Moreover, we demonstrated this chondrogenesis-inducing primarily attributed miR-140, one most abundant LIPUS-BMSC-Exos. LIPUS-preconditioned ameliorate by positive regulation pro-chondrogenesis anti-adipogenesis, which delivering miR-140. These findings propose an innovative "LIPUS combined Exosomes strategy" combines both physiotherapeutic biotherapeutic advantages. This strategy possesses good translational as local LIPUS preconditioned BMSC-derived Exos during operation not only efficient promoting ameliorating infiltration, but time-saving, simple convenient patients.

Language: Английский

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